Click here to switch to the map view.

The map label for this gene is azoR2 [H]

Identifier: 113968485

GI number: 113968485

Start: 158518

End: 159207

Strand: Direct

Name: azoR2 [H]

Synonym: Shewmr4_0140

Alternate gene names: 113968485

Gene position: 158518-159207 (Clockwise)

Preceding gene: 113968482

Following gene: 113968486

Centisome position: 3.37

GC content: 46.23

Gene sequence:

>690_bases
ATGACACATATTCTTCATATAGATTCAAGCGTTAGAGCTGTGTTAAACGATAATCCAGCTCATAACTCTATTTCTAAACA
ATTGGCAGAACGGTTTATTGCTAGTTTTAAAACTCACTGTGCTACAGCTGAGTATACTTACCGTGATGTCGGGATGAATC
CACCAGATTTTATCAACCAACAGTGGATTGGTGCGGTGTTCACGCCGGAGGCTAAACGCTCTGCGGCGCAGCGGCAGCAA
TTGGCATTATCGGATCAATTGATTGCGGAGGTGTCAGCGGCCGATCTGATTGTGATTTCTGCACCTATGTACAACTACGG
TATGCCTGCTCAGCTAAAAGCTTGGTTTGATCAGATTGTAAGGATTAACAAGACCTTCGATTTTGACTTGAACCGTGGCG
ATACCCCTCTAGCGCCATTACTGTCAGGCAAAACCTTAGTGATTCTGACGTCCTGCGGTGAGTTCGGCTTTGAGGAGGGT
GGCGTAAACGAACGGCATGGGCATTTGGTGCCGCATCTACGTACCCTAAGTAAGTATTTGGGTGTTGAGCAGATTTTTGA
AGTCGCCTCTGAATATCAAGAGTTTGCCGATAATCGACATCAGCAATCGTTACAACAGGCTCTGGCAAAGTCCGCTGCCA
TGGGGTTACAACTAGCACAACACTACGCAGGGAAAACGACTGATGTGTAA

Upstream 100 bases:

>100_bases
AGATGAGTTTGGCTCATCTGTAAATGCGTTTTAGTCGTTTGTCTGTCACTCGCTGAAAAACCACAATAGCTGGGTATTTT
CAGCTAAAGAGAGATTCAAG

Downstream 100 bases:

>100_bases
TCACTTTTTACCTGCGGTGAACCACACCTTGGCGTTGGCGCAAAAGGAAGTCGAAGGTACAGGCCTGCCGTTTTCGGCTG
TGATTGTGGATGAGTCTGGG

Product: (Acyl-carrier-protein) phosphodiesterase

Products: 4'- Phosphopantetheine; Apoprotein. [C]

Alternate protein names: Azo-dye reductase 2; FMN-dependent NADH-azo compound oxidoreductase 2 [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTHILHIDSSVRAVLNDNPAHNSISKQLAERFIASFKTHCATAEYTYRDVGMNPPDFINQQWIGAVFTPEAKRSAAQRQQ
LALSDQLIAEVSAADLIVISAPMYNYGMPAQLKAWFDQIVRINKTFDFDLNRGDTPLAPLLSGKTLVILTSCGEFGFEEG
GVNERHGHLVPHLRTLSKYLGVEQIFEVASEYQEFADNRHQQSLQQALAKSAAMGLQLAQHYAGKTTDV

Sequences:

>Translated_229_residues
MTHILHIDSSVRAVLNDNPAHNSISKQLAERFIASFKTHCATAEYTYRDVGMNPPDFINQQWIGAVFTPEAKRSAAQRQQ
LALSDQLIAEVSAADLIVISAPMYNYGMPAQLKAWFDQIVRINKTFDFDLNRGDTPLAPLLSGKTLVILTSCGEFGFEEG
GVNERHGHLVPHLRTLSKYLGVEQIFEVASEYQEFADNRHQQSLQQALAKSAAMGLQLAQHYAGKTTDV
>Mature_228_residues
THILHIDSSVRAVLNDNPAHNSISKQLAERFIASFKTHCATAEYTYRDVGMNPPDFINQQWIGAVFTPEAKRSAAQRQQL
ALSDQLIAEVSAADLIVISAPMYNYGMPAQLKAWFDQIVRINKTFDFDLNRGDTPLAPLLSGKTLVILTSCGEFGFEEGG
VNERHGHLVPHLRTLSKYLGVEQIFEVASEYQEFADNRHQQSLQQALAKSAAMGLQLAQHYAGKTTDV

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=191, Percent_Identity=32.4607329842932, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14 [C]

Molecular weight: Translated: 25446; Mature: 25315

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHILHIDSSVRAVLNDNPAHNSISKQLAERFIASFKTHCATAEYTYRDVGMNPPDFINQ
CCEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCH
QWIGAVFTPEAKRSAAQRQQLALSDQLIAEVSAADLIVISAPMYNYGMPAQLKAWFDQIV
HHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHH
RINKTFDFDLNRGDTPLAPLLSGKTLVILTSCGEFGFEEGGVNERHGHLVPHLRTLSKYL
HHCCCEEECCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GVEQIFEVASEYQEFADNRHQQSLQQALAKSAAMGLQLAQHYAGKTTDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
THILHIDSSVRAVLNDNPAHNSISKQLAERFIASFKTHCATAEYTYRDVGMNPPDFINQ
CEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCH
QWIGAVFTPEAKRSAAQRQQLALSDQLIAEVSAADLIVISAPMYNYGMPAQLKAWFDQIV
HHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHH
RINKTFDFDLNRGDTPLAPLLSGKTLVILTSCGEFGFEEGGVNERHGHLVPHLRTLSKYL
HHCCCEEECCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
GVEQIFEVASEYQEFADNRHQQSLQQALAKSAAMGLQLAQHYAGKTTDV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [Acyl-Carrier Protein]; H2O [C]

Specific reaction: [Acyl-Carrier Protein] + H2O = 4'- Phosphopantetheine + Apoprotein. [C]

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA