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The map label for this gene is fdnG [H]

Identifier: 113968447

GI number: 113968447

Start: 113739

End: 116123

Strand: Direct

Name: fdnG [H]

Synonym: Shewmr4_0102

Alternate gene names: 113968447

Gene position: 113739-116123 (Clockwise)

Preceding gene: 113968446

Following gene: 113968448

Centisome position: 2.42

GC content: 50.19

Gene sequence:

>2385_bases
ATGACCAACCACTGGATCGATATTAAAAATTCTAACGTAGTGATTATTATGGGCGGTAATGCCGCAGAGGCTCACCCTGT
CGGATTCGGCTGGGTTACAGAAGCTATGCAGCATAACAATGCTAAGTTGATTGTGGTTGATCCACGTTTCAACCGCAGTG
CGTCATTGGCAGATCACTATGCCCCAATCCGCTCAGGAACTGACATAGCATTCCTTCTGGGTGTGATTCGCTATTTGATT
TCGACCAATCAAGTTAACTTCGATTATGTGAAAGCCTATACCAACGCCAGCTATCTGGTGCGGGATGATTTTGACTTCCA
TGACGGCCTATTCTCAGGCTTTGATGAAGCAAAAGGCGAGTACAACAAAGAATCCTGGTTCTACCAACTCGACGAAGACG
GCTATGCCAAAGTGGATGAAACACTTGAGCACCCGCGTTGTGTGTGGAACTTACTCAAACAACACGTAGAACGTTATGAT
TTTGCAACGGTAAGTAATATCACTGGTACGCCAACAGCGGACTACCAAGTGGTATGTGACGCCATTGCGAGCACTCATAC
TAAAGATCGTGTGGCAACCTTTATGTATGCGCTGGGCTGGACCCACCATAGTAAAGGTGCGCAGAACATCCGTTCTATGG
CGATGATCCAGTTATTACTGGGCAACATTGGTCAGTTAGGCGGTGGTGTTAACGCATTGCGTGGTCACGCCAACGTTCAA
GGTTCAACCGACATGGGGCTACTGGCTCAAAGTTTACCCGGTTACCTTAAACTGCCAAACGATAAAGAACCAACCTTAGC
GGCTCACTTGGCGGCGAATACGCCTAAGCCATTGCGCCCAGGCCAAACCAACTACTGGCAAAACTATCCGAAGTTTTATG
TGTCCCTGCTTAAAGCCTTCTGGGGTGAAAACGCGACGCCTGAAAACGAGTTCGGTTATCAGTGGTTACCTAAGTGGGAT
CAGATGTATGACTTCGGTAAGCATCTGGACATGATGTACCGTGGCAAAGTGAACGGTTGCATCGTGCAAGGGGTTAACGC
CATTAACTCCATGCCTAACCGTAACAAGAACATCAAGGCGCTGAGTAACCTTAAGTACTTAGTGGTGCTTGATAACTTGT
CATCGGAAACCGCCACCTTCTGGCAAAACGAACCCGGTTTTAACGAGGTCGATACCGCCAGTATTCAAACCGAGGTCTTC
CGCCTACCTGCGACCGTGTTCGCAGAGGAAGAAGGTTCGATCGTTAACTCTGGCCGCTGGATGCAATGGCACTATAAAGG
CGCTAATCCTCCAGGCGAAGCAATGTCGGACTCAGAGATTGTCTCTGGTCTGCTGCAGGAGCTAAAACACCTCTATCGCG
AAGAGGGTGGCAAACTGCCTGAACCCATTGAAGCGATTAACTGGAACTATATCGATCCGCACAACCCAAGCTCTATCGAA
CTGACGAAAGAGTTAAATGGTTACGATGTCGCGACTAAGCGTCAGATCAGCAGCTTTGCTGAACTGAAGGCCGATGGTAG
TACCGCGAGTGCCTGTTGGATTTACGCGGGGTCTTGGACCGAAGCCGGTAACCAAATGGCTCGCCGCGATAACCATGACC
CATCGGGTAAAGGTATTACGCCGGGCTGGGCCTTCGCATGGCCGCTCAACCGCCGCGTCCTCTACAACCGTGCCTCTTGT
GATGTGAACGGTAAACCTTGGGATGAGCACCGTAAGATTGTTGAGTGGAAAGACGGCAAGTGGGAAGGGATAGACGTGCC
CGACTTCAATGCCAAGTTGAATCCACAGGAAAGTGCGCATCCTTTCATTATGCAGGCTGAAGGCGTAGGTCGTTTCTTCG
CGCTCAAACTGCTTAAAGAGGGACCCTTCCCAGAGCATTACGAGCCTGTCGAATCGCCCATCGGCACCAACCCACTGCAT
CCCAATGTGGTGCACAGCCCTGTGCTGCGTTGGTTCGAAGGGGTGAAAGACACCATAGGTACTAAAGAGGAATATCCCTA
CGCCTGTACCACTTACTCACTTACAGAACACTTTAACTTCTGGACGACACACTGCCGTTTAGCGGCGATTGCCATGCCAG
AGACCTTTGTGGAAATGAATGAGCAATTGGCGGCAGAAAAAGGCATTAAGAATGGCGATTGGGTGAAAGTGAGCTCGAAA
CGTGGCCACATTCTCACCAAAGCCTTAGTGACTAAGCGTATGCGCCCATTGCAGGTCAATGGTCAAACGGTACACACACT
CGGTATCCCACGTCACGGCAGCCATAACGCGCTAACACGTAAGGCCTATAGCTGTAACGTCCTTACCACTGAGATGGGGG
ATGCCAACACGGGTGTGCCTGAGTACAAAGCGTTCCTCGTGAATGTTGAAAAGGCGGAGGTCTGA

Upstream 100 bases:

>100_bases
ATTTGCGCGCGCTTTAGGCCTAGTGGCTATCGATACTATTGCACGTAACTGACACTCCCCAACGGTAGCAAGTCTTGCTC
CAACATTTGGGCGCGGTGCC

Downstream 100 bases:

>100_bases
TAATGGCGACTCAAGATATCATTCAACGTTCCGGCACTTCGCAAGCGACTCCGCCTGCACAGGCGAGAGTCGGTGGCGTG
AAGCAAATTGCGAAGCTGTT

Product: formate dehydrogenase subunit alpha

Products: NA

Alternate protein names: Anaerobic formate dehydrogenase major subunit; Formate dehydrogenase-N subunit alpha; FDH-N subunit alpha [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MTNHWIDIKNSNVVIIMGGNAAEAHPVGFGWVTEAMQHNNAKLIVVDPRFNRSASLADHYAPIRSGTDIAFLLGVIRYLI
STNQVNFDYVKAYTNASYLVRDDFDFHDGLFSGFDEAKGEYNKESWFYQLDEDGYAKVDETLEHPRCVWNLLKQHVERYD
FATVSNITGTPTADYQVVCDAIASTHTKDRVATFMYALGWTHHSKGAQNIRSMAMIQLLLGNIGQLGGGVNALRGHANVQ
GSTDMGLLAQSLPGYLKLPNDKEPTLAAHLAANTPKPLRPGQTNYWQNYPKFYVSLLKAFWGENATPENEFGYQWLPKWD
QMYDFGKHLDMMYRGKVNGCIVQGVNAINSMPNRNKNIKALSNLKYLVVLDNLSSETATFWQNEPGFNEVDTASIQTEVF
RLPATVFAEEEGSIVNSGRWMQWHYKGANPPGEAMSDSEIVSGLLQELKHLYREEGGKLPEPIEAINWNYIDPHNPSSIE
LTKELNGYDVATKRQISSFAELKADGSTASACWIYAGSWTEAGNQMARRDNHDPSGKGITPGWAFAWPLNRRVLYNRASC
DVNGKPWDEHRKIVEWKDGKWEGIDVPDFNAKLNPQESAHPFIMQAEGVGRFFALKLLKEGPFPEHYEPVESPIGTNPLH
PNVVHSPVLRWFEGVKDTIGTKEEYPYACTTYSLTEHFNFWTTHCRLAAIAMPETFVEMNEQLAAEKGIKNGDWVKVSSK
RGHILTKALVTKRMRPLQVNGQTVHTLGIPRHGSHNALTRKAYSCNVLTTEMGDANTGVPEYKAFLVNVEKAEV

Sequences:

>Translated_794_residues
MTNHWIDIKNSNVVIIMGGNAAEAHPVGFGWVTEAMQHNNAKLIVVDPRFNRSASLADHYAPIRSGTDIAFLLGVIRYLI
STNQVNFDYVKAYTNASYLVRDDFDFHDGLFSGFDEAKGEYNKESWFYQLDEDGYAKVDETLEHPRCVWNLLKQHVERYD
FATVSNITGTPTADYQVVCDAIASTHTKDRVATFMYALGWTHHSKGAQNIRSMAMIQLLLGNIGQLGGGVNALRGHANVQ
GSTDMGLLAQSLPGYLKLPNDKEPTLAAHLAANTPKPLRPGQTNYWQNYPKFYVSLLKAFWGENATPENEFGYQWLPKWD
QMYDFGKHLDMMYRGKVNGCIVQGVNAINSMPNRNKNIKALSNLKYLVVLDNLSSETATFWQNEPGFNEVDTASIQTEVF
RLPATVFAEEEGSIVNSGRWMQWHYKGANPPGEAMSDSEIVSGLLQELKHLYREEGGKLPEPIEAINWNYIDPHNPSSIE
LTKELNGYDVATKRQISSFAELKADGSTASACWIYAGSWTEAGNQMARRDNHDPSGKGITPGWAFAWPLNRRVLYNRASC
DVNGKPWDEHRKIVEWKDGKWEGIDVPDFNAKLNPQESAHPFIMQAEGVGRFFALKLLKEGPFPEHYEPVESPIGTNPLH
PNVVHSPVLRWFEGVKDTIGTKEEYPYACTTYSLTEHFNFWTTHCRLAAIAMPETFVEMNEQLAAEKGIKNGDWVKVSSK
RGHILTKALVTKRMRPLQVNGQTVHTLGIPRHGSHNALTRKAYSCNVLTTEMGDANTGVPEYKAFLVNVEKAEV
>Mature_793_residues
TNHWIDIKNSNVVIIMGGNAAEAHPVGFGWVTEAMQHNNAKLIVVDPRFNRSASLADHYAPIRSGTDIAFLLGVIRYLIS
TNQVNFDYVKAYTNASYLVRDDFDFHDGLFSGFDEAKGEYNKESWFYQLDEDGYAKVDETLEHPRCVWNLLKQHVERYDF
ATVSNITGTPTADYQVVCDAIASTHTKDRVATFMYALGWTHHSKGAQNIRSMAMIQLLLGNIGQLGGGVNALRGHANVQG
STDMGLLAQSLPGYLKLPNDKEPTLAAHLAANTPKPLRPGQTNYWQNYPKFYVSLLKAFWGENATPENEFGYQWLPKWDQ
MYDFGKHLDMMYRGKVNGCIVQGVNAINSMPNRNKNIKALSNLKYLVVLDNLSSETATFWQNEPGFNEVDTASIQTEVFR
LPATVFAEEEGSIVNSGRWMQWHYKGANPPGEAMSDSEIVSGLLQELKHLYREEGGKLPEPIEAINWNYIDPHNPSSIEL
TKELNGYDVATKRQISSFAELKADGSTASACWIYAGSWTEAGNQMARRDNHDPSGKGITPGWAFAWPLNRRVLYNRASCD
VNGKPWDEHRKIVEWKDGKWEGIDVPDFNAKLNPQESAHPFIMQAEGVGRFFALKLLKEGPFPEHYEPVESPIGTNPLHP
NVVHSPVLRWFEGVKDTIGTKEEYPYACTTYSLTEHFNFWTTHCRLAAIAMPETFVEMNEQLAAEKGIKNGDWVKVSSKR
GHILTKALVTKRMRPLQVNGQTVHTLGIPRHGSHNALTRKAYSCNVLTTEMGDANTGVPEYKAFLVNVEKAEV

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868719, Length=804, Percent_Identity=62.3134328358209, Blast_Score=1048, Evalue=0.0,
Organism=Escherichia coli, GI3868720, Length=804, Percent_Identity=58.7064676616915, Blast_Score=1010, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=450, Percent_Identity=23.3333333333333, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI171474008, Length=251, Percent_Identity=29.8804780876494, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081797, Length=239, Percent_Identity=29.2887029288703, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1787870, Length=238, Percent_Identity=28.9915966386555, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006443
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 89188; Mature: 89057

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNHWIDIKNSNVVIIMGGNAAEAHPVGFGWVTEAMQHNNAKLIVVDPRFNRSASLADHY
CCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
APIRSGTDIAFLLGVIRYLISTNQVNFDYVKAYTNASYLVRDDFDFHDGLFSGFDEAKGE
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHEEECCCEEEEECCCCHHHHHHHCHHHHCCC
YNKESWFYQLDEDGYAKVDETLEHPRCVWNLLKQHVERYDFATVSNITGTPTADYQVVCD
CCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHH
AIASTHTKDRVATFMYALGWTHHSKGAQNIRSMAMIQLLLGNIGQLGGGVNALRGHANVQ
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
GSTDMGLLAQSLPGYLKLPNDKEPTLAAHLAANTPKPLRPGQTNYWQNYPKFYVSLLKAF
CCCCHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHH
WGENATPENEFGYQWLPKWDQMYDFGKHLDMMYRGKVNGCIVQGVNAINSMPNRNKNIKA
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCCHH
LSNLKYLVVLDNLSSETATFWQNEPGFNEVDTASIQTEVFRLPATVFAEEEGSIVNSGRW
HHCCEEEEEEECCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCEECCCCE
MQWHYKGANPPGEAMSDSEIVSGLLQELKHLYREEGGKLPEPIEAINWNYIDPHNPSSIE
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCEE
LTKELNGYDVATKRQISSFAELKADGSTASACWIYAGSWTEAGNQMARRDNHDPSGKGIT
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCC
PGWAFAWPLNRRVLYNRASCDVNGKPWDEHRKIVEWKDGKWEGIDVPDFNAKLNPQESAH
CCCEEECCCCCEEEEECCCCCCCCCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PFIMQAEGVGRFFALKLLKEGPFPEHYEPVESPIGTNPLHPNVVHSPVLRWFEGVKDTIG
CEEEEECCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TKEEYPYACTTYSLTEHFNFWTTHCRLAAIAMPETFVEMNEQLAAEKGIKNGDWVKVSSK
CCCCCCEEEEEEEHHHCCCHHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECC
RGHILTKALVTKRMRPLQVNGQTVHTLGIPRHGSHNALTRKAYSCNVLTTEMGDANTGVP
CCCHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCHHHHCEEECEEEEECCCCCCCCCC
EYKAFLVNVEKAEV
CEEEEEEEEHHCCC
>Mature Secondary Structure 
TNHWIDIKNSNVVIIMGGNAAEAHPVGFGWVTEAMQHNNAKLIVVDPRFNRSASLADHY
CCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
APIRSGTDIAFLLGVIRYLISTNQVNFDYVKAYTNASYLVRDDFDFHDGLFSGFDEAKGE
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHEEECCCEEEEECCCCHHHHHHHCHHHHCCC
YNKESWFYQLDEDGYAKVDETLEHPRCVWNLLKQHVERYDFATVSNITGTPTADYQVVCD
CCCCCEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHH
AIASTHTKDRVATFMYALGWTHHSKGAQNIRSMAMIQLLLGNIGQLGGGVNALRGHANVQ
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
GSTDMGLLAQSLPGYLKLPNDKEPTLAAHLAANTPKPLRPGQTNYWQNYPKFYVSLLKAF
CCCCHHHHHHHCCCCEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHH
WGENATPENEFGYQWLPKWDQMYDFGKHLDMMYRGKVNGCIVQGVNAINSMPNRNKNIKA
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCCHH
LSNLKYLVVLDNLSSETATFWQNEPGFNEVDTASIQTEVFRLPATVFAEEEGSIVNSGRW
HHCCEEEEEEECCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCEECCCCE
MQWHYKGANPPGEAMSDSEIVSGLLQELKHLYREEGGKLPEPIEAINWNYIDPHNPSSIE
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCEE
LTKELNGYDVATKRQISSFAELKADGSTASACWIYAGSWTEAGNQMARRDNHDPSGKGIT
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCC
PGWAFAWPLNRRVLYNRASCDVNGKPWDEHRKIVEWKDGKWEGIDVPDFNAKLNPQESAH
CCCEEECCCCCEEEEECCCCCCCCCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
PFIMQAEGVGRFFALKLLKEGPFPEHYEPVESPIGTNPLHPNVVHSPVLRWFEGVKDTIG
CEEEEECCCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
TKEEYPYACTTYSLTEHFNFWTTHCRLAAIAMPETFVEMNEQLAAEKGIKNGDWVKVSSK
CCCCCCEEEEEEEHHHCCCHHHHHEEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEECC
RGHILTKALVTKRMRPLQVNGQTVHTLGIPRHGSHNALTRKAYSCNVLTTEMGDANTGVP
CCCHHHHHHHHHCCCCEEECCEEEEEECCCCCCCCCHHHHCEEECEEEEECCCCCCCCCC
EYKAFLVNVEKAEV
CEEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]