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The map label for this gene is gyrB [H]

Identifier: 113968350

GI number: 113968350

Start: 3843

End: 6260

Strand: Direct

Name: gyrB [H]

Synonym: Shewmr4_0004

Alternate gene names: 113968350

Gene position: 3843-6260 (Clockwise)

Preceding gene: 113968349

Following gene: 113968352

Centisome position: 0.08

GC content: 48.59

Gene sequence:

>2418_bases
ATGTCAGAGAATAGTTACGATTCTTCGAGTATTAAGGTCCTAAAGGGCCTTGATGCAGTACGTAAGAGACCTGGGATGTA
TATTGGTGACACCGACGACGGTACGGGTCTACATCATATGGTGTTCGAAGTAGTCGATAACTCTATCGATGAAGCCTTAG
CCGGCCATTGTACTGATATCACTATTACTATCCATGTGGATGGTTCGGTCTCCGTTCGCGACGACGGCCGTGGTATTCCT
GTGGCGATTCACCCGGAAGAAGGCGTGTCTGCCGCCGAGGTGATCATGACGGTACTGCACGCGGGCGGTAAGTTCGACGA
TAACTCTTATAAAGTGTCAGGTGGTCTGCACGGCGTGGGTGTATCGGTAGTTAACGCGCTGTCTAAAAAGCTGCAGTTAA
CCATTCGCCGCGAAGGAAAAGTCCACGAGCAGTTTTATACCCACGGTGTACCTGACGAGCCAATCAAAGTGATCGGCGAT
GCGACTAAAACCGGTACCGAAATCCGTTTCTGGCCAAGTGAAGAAACCTTCAGCGATGTTGAGTTCCACTTCGAGATTCT
GGCAAAGCGCGTGCGCGAACTGTCATTCCTCAACTCAGGCGTGGGTATCCGCTTAGTCGACGAGCGTGACAATAAGAATG
AATTCTTTAAATACGAAGGCGGTATCAGCGCATTCGTTGATTACCTAAACCGTAATAAAACGCCAGTAAACAAAGACGTA
TTCCACTTTATGCAAGAGCGTGAAGATGGCATTACCGTTGAAGTGGCGATGCAGTGGAACGATGGTTACCAAGAAAGCAT
TTTCTGTTTTACCAACAACATTCCACAGCGTGATGGTGGTACTCACCTAGCGGGTTTCCGTAGTGCGTTGACACGTAACC
TCAACAACTACATGGAAAACGAAGGTTATAACAAGAAAGGCAAAACTAACGCGACAGGCGATGACGCCCGCGAAGGTTTA
ACGGCCGTGATTTCAGTTAAAGTGCCTGATCCTAAGTTCAGCTCGCAAACTAAAGACAAGTTAGTCTCTAGCGAAGTGAA
AGCCGCGGTTGAACAAACCATGGGTGAAAAGCTAAACGACTATCTGCTGGAAAACCCTGCCGATGCTAAGTTAATCGTCG
GTAAGATTGTCGATGCGGCCCGTGCCCGTGAAGCGGCGCGTAAAGCCCGTGAAATGACCCGTCGTAAAGGCGCACTCGAT
TTAGGTGGTTTGCCAGGTAAGCTGGCGGATTGCCAAGAGAAAGACCCTGGTCTTTCAGAAATCTACATAGTGGAAGGGGA
CTCTGCTGGCGGTAGCGCTAAGCAGGGACGTAACCGTAAGAACCAAGCGATTCTGCCACTGAAAGGTAAAATCTTAAACG
TTGAGAAAGCGCGTTTCGATAAAATGCTTTCTTCTCAAGAGGTTGCAACGCTAATTACCGCACTTGGCTGTGGTATTGGT
CGCGACGAATACAACCCGGATAAGACGCGTTACCACAACATCATCATCATGACGGATGCCGACGTCGACGGCTCGCACAT
TCGTACCTTGCTGTTGACCTTCTTCTTCCGCCAAATGCCTGAACTGATTGAACGTGGTTATGTGTATATTGCTCAGCCGC
CTTTATTCAAAGTGAAGAAAGGTAAGCAAGAACAATATCTCAAAGACGAAGCAGCACTGACTCAGTATCTGACCACTCAA
GCGTTAGATGGTACTAGCATTTACCCATCTCAAGGTGCGCCAGGTATGTCGGGTGAACCATTAGAGCGTTTAGTGACTCA
GTATCGTGAAGTGGAAGCGATTGTTGCTCGTTTAGAGCAACGTTACCCAACGCAGATCACCAACCGCATGCTTTATCATC
CAATGATCAACAACGAGATGTTGGCGGATGAAGGCAAGATGAAGGAATGGATCGACGCCTTTATCCATGAGCTGGTGGAA
TTAGAAAACAGCGGTGTGTTGTATTCTGGTGAGCCTGTACTCGATCCTGAGCGTAAAGTGTATCTGCCGAAGATCACTAT
CCGTAAACACGGTATCGATACACACTACCTGTTCAGCTACGATTTCTTCCAATCAACTGATTACCAACGCATTGGTAAGT
TAGGTGCGGCGCTCGAAGGCCTGATTGAAGTCGGTGGTTATGTACAGCGTGGCGACCGTGTGAAGGAAGTGGGTAGCTTC
ATTGAAGCGTTAGATTGGACCATCGGCGAAGCCAAACGTGGCCTCTATATCCAACGCTATAAAGGATTGGGTGAGATGAA
CCCTGAGCAATTGTGGGAAACCACGATGGATCCTGAATCTCGCCGCATGCTGCAAGTGACGATTGATGATGCCGTTGGTG
CGGATCAGCTGTTTACTTGTTTGATGGGCGACCAAGTGGAACCACGCCGTGACTTTATCGAAGCCAACGCGCTGAACGTG
GCTAACTTAGACGTTTAA

Upstream 100 bases:

>100_bases
CCTGCAGCAATAGTCGATTCGTTACACACGCCTCCCAGTAGGATGTTCCATGTGGAACAGGGACGTGTAACGGTAATTGA
ATAACCATTGAGAGAATAAT

Downstream 100 bases:

>100_bases
CATCCAGACGTTTAACATCTATATCGTTAGTGATTGATAAAAAGGGAAAGCTTAGCTTTCCCTTTTTGTTTTATTGCAAC
CTATCTATCAACACCTGCCT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDITITIHVDGSVSVRDDGRGIP
VAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGD
ATKTGTEIRFWPSEETFSDVEFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
FHFMQEREDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEAALTQYLTTQ
ALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTQITNRMLYHPMINNEMLADEGKMKEWIDAFIHELVE
LENSGVLYSGEPVLDPERKVYLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRRDFIEANALNV
ANLDV

Sequences:

>Translated_805_residues
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDITITIHVDGSVSVRDDGRGIP
VAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGD
ATKTGTEIRFWPSEETFSDVEFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
FHFMQEREDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGL
TAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALD
LGGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEAALTQYLTTQ
ALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTQITNRMLYHPMINNEMLADEGKMKEWIDAFIHELVE
LENSGVLYSGEPVLDPERKVYLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRRDFIEANALNV
ANLDV
>Mature_804_residues
SENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDITITIHVDGSVSVRDDGRGIPV
AIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGDA
TKTGTEIRFWPSEETFSDVEFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDVF
HFMQEREDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMENEGYNKKGKTNATGDDAREGLT
AVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLNDYLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDL
GGLPGKLADCQEKDPGLSEIYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKKGKQEQYLKDEAALTQYLTTQA
LDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQRYPTQITNRMLYHPMINNEMLADEGKMKEWIDAFIHELVEL
ENSGVLYSGEPVLDPERKVYLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSFI
EALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTCLMGDQVEPRRDFIEANALNVA
NLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=550, Percent_Identity=25.0909090909091, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI19913406, Length=550, Percent_Identity=24, Blast_Score=114, Evalue=4e-25,
Organism=Escherichia coli, GI48994957, Length=803, Percent_Identity=70.9838107098381, Blast_Score=1189, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=548, Percent_Identity=40.1459854014599, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=538, Percent_Identity=26.0223048327138, Blast_Score=132, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=507, Percent_Identity=23.6686390532544, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.9869281045752, Blast_Score=70, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=582, Percent_Identity=25.4295532646048, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI17136538, Length=535, Percent_Identity=26.7289719626168, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89913; Mature: 89782

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDI
CCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
TITIHVDGSVSVRDDGRGIPVAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGDATKTGTEIRFWPSEETFSDV
HHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHH
EFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHH
FHFMQEREDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMEN
HHHHHHCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EGYNKKGKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKK
CCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
GKQEQYLKDEAALTQYLTTQALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQ
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPTQITNRMLYHPMINNEMLADEGKMKEWIDAFIHELVELENSGVLYSGEPVLDPERKV
HCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEE
YLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
EECEEEEEECCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTC
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDQVEPRRDFIEANALNVANLDV
HHCCCCCCHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
SENSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCTDI
CCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
TITIHVDGSVSVRDDGRGIPVAIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
EEEEEECCEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSKKLQLTIRREGKVHEQFYTHGVPDEPIKVIGDATKTGTEIRFWPSEETFSDV
HHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCHHHCCCCCCCCCEEEECCCCCCHHHH
EFHFEILAKRVRELSFLNSGVGIRLVDERDNKNEFFKYEGGISAFVDYLNRNKTPVNKDV
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCHHHHHHHHHCCCCCCCHHH
FHFMQEREDGITVEVAMQWNDGYQESIFCFTNNIPQRDGGTHLAGFRSALTRNLNNYMEN
HHHHHHCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHC
EGYNKKGKTNATGDDAREGLTAVISVKVPDPKFSSQTKDKLVSSEVKAAVEQTMGEKLND
CCCCCCCCCCCCCCHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YLLENPADAKLIVGKIVDAARAREAARKAREMTRRKGALDLGGLPGKLADCQEKDPGLSE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
IYIVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIG
EEEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCC
RDEYNPDKTRYHNIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYVYIAQPPLFKVKK
CCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECCC
GKQEQYLKDEAALTQYLTTQALDGTSIYPSQGAPGMSGEPLERLVTQYREVEAIVARLEQ
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RYPTQITNRMLYHPMINNEMLADEGKMKEWIDAFIHELVELENSGVLYSGEPVLDPERKV
HCCHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEE
YLPKITIRKHGIDTHYLFSYDFFQSTDYQRIGKLGAALEGLIEVGGYVQRGDRVKEVGSF
EECEEEEEECCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
IEALDWTIGEAKRGLYIQRYKGLGEMNPEQLWETTMDPESRRMLQVTIDDAVGADQLFTC
HHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHH
LMGDQVEPRRDFIEANALNVANLDV
HHCCCCCCHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]