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The map label for this gene is cspA [H]

Identifier: 116696515

GI number: 116696515

Start: 2911913

End: 2912209

Strand: Direct

Name: cspA [H]

Synonym: H16_B2579

Alternate gene names: 116696515

Gene position: 2911913-2912209 (Clockwise)

Preceding gene: 116696513

Following gene: NA

Centisome position: 99.98

GC content: 58.25

Gene sequence:

>297_bases
ATGACGTTTTCGTCATGCGATGTTTTTTGTCCTTTTTCTGGAAAGCAAATCATGCAAACCGGTATCGTCAAGTGGTTCAA
CGACGCCAAGGGCTTCGGCTTCATCAAGCCGGACGCGGGTGGTGACGACCTCTTCGCCCATTTCTCGGAAATCCGCGCCG
ACGGCTTCAAGTCGCTGCAGGAAAACCAGCGCGTGCAGTTCGAAGTCAAGAATGGCCCGAAGGGCCTGCAGGCCGCGAAC
ATCACCCCGCTGTAAGCATTGACTGCCGGCGCGCCCCGCGCGCGCCGAACAGCGTAG

Upstream 100 bases:

>100_bases
AGCCGCCTAGATTCAAGCGACGAGGCAACAGTTCATTCGTTAGCCACCGTCTGGTACTTCGGTTGTCCATGGTGTCCTTT
CCTTGAGTTCCCCGTGTCGG

Downstream 100 bases:

>100_bases
AACCCCGCCCATGGCGGGGTTTTTTGTTTTCAGGCGCGGATAGCGCGATTGGCGGTGCTGTCTGCGCCACCTTCATCACA
TCCCGGCCCCCGCCGCTCCC

Product: cold shock protein, DNA-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 97

Protein sequence:

>98_residues
MTFSSCDVFCPFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRADGFKSLQENQRVQFEVKNGPKGLQAAN
ITPLXALTAGAPRARRTA

Sequences:

>Translated_98_residues
MTFSSCDVFCPFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRADGFKSLQENQRVQFEVKNGPKGLQAAN
ITPL*ALTAGAPRARRTA
>Mature_97_residues
TFSSCDVFCPFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRADGFKSLQENQRVQFEVKNGPKGLQAANI
TPL*ALTAGAPRARRTA

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=71, Percent_Identity=54.9295774647887, Blast_Score=92, Evalue=9e-21,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=59.375, Blast_Score=86, Evalue=6e-19,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=60, Blast_Score=85, Evalue=8e-19,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=55.3846153846154, Blast_Score=82, Evalue=9e-18,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=52.3076923076923, Blast_Score=80, Evalue=2e-17,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=54.6875, Blast_Score=79, Evalue=6e-17,
Organism=Escherichia coli, GI1787839, Length=63, Percent_Identity=52.3809523809524, Blast_Score=73, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24658520, Length=66, Percent_Identity=42.4242424242424, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 10552; Mature: 10421

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSSCDVFCPFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRADGFKSLQ
CCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
ENQRVQFEVKNGPKGLQAANITPLALTAGAPRARRTA
CCCEEEEEECCCCCCCEECCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
TFSSCDVFCPFSGKQIMQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEIRADGFKSLQ
CCCCCCEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
ENQRVQFEVKNGPKGLQAANITPLALTAGAPRARRTA
CCCEEEEEECCCCCCCEECCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]