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The map label for this gene is paaF [C]

Identifier: 116696470

GI number: 116696470

Start: 2862976

End: 2863752

Strand: Direct

Name: paaF [C]

Synonym: H16_B2534

Alternate gene names: 116696470

Gene position: 2862976-2863752 (Clockwise)

Preceding gene: 116696469

Following gene: 116696472

Centisome position: 98.3

GC content: 69.88

Gene sequence:

>777_bases
ATGAGCGCACTTCAGCAAGAACCGCACGCCGGCGTCGAGATCGACGCGCGCGGCATCGCCACCGTCACCATCCGCGAGGC
GGGCTCGCTCAATATCCTGAGCACGCCGGTGATCGCATCGCTCACGCGCGCCATCGAAGCGCTGGCCGGCAATGACGCGG
TACGCGTGCTGGTGCTGCGCGGCGCCGGCGAGAAAGGCTTTGTCGGCGGTGCCGACATCAAGGAAATGGCCGCACTGGAC
CGCGCCAGCGCCGAGGCCTTCATCAGCGGCCTGCGCCGCCTGTGCGACGCCGTGCGCCACCTGCCGGTGCCGGTGATCGC
GCGCATGCCGGGCTGGTGCCTGGGCGGCGGGCTGGAACTGGCGCTGGCGTGCGATATCCGCATCGCCGCCGACAACGCGC
AGCTGGGCATGCCGGAAGTGAAGGTCGGCATCCCGTCGGTGATCCACGCCGCGCTGATGCCGCGCCTGATCGGCAATGCG
CGCACGGCGTGGATGCTGCTGACGGGCGAGATCACCGATGCCGCCGAGGCGCTGCAATGGGGGCTGGTGAGCCGCGTGGT
GCCGCTGGCGGAGCTGGACCTGGAAATCGAACGCGTGGCCGCGCTGCTGGCCGGCTTCGGCCCGGTCGCGGTGCGCCAGC
AGAAGCGCCTGCTGCGCGAGTGGGAAGATGCGCCGCTGGAAGTCTCGATCAACAACAGCGTGACCGAGTTCGGCAGCGCC
TTCGACACCGGCGAGCCACAGCAGCACATGGCCACCTTCCTGAACCGCAAGCGCTGA

Upstream 100 bases:

>100_bases
CAAGGCCTTCGGCGACCTGATCCGGGCGCAGAATGCGCACTGGAAGCAAGTCGTCGAGGACATCCATTTCACCAGGCTCC
AGTAAGCCACAAGGAACGAC

Downstream 100 bases:

>100_bases
AGCGCGGGGAAAGTGTTTACGGCGCCGTGGCTGCCACGGCGCCGCCTTCGCGCGTGGCCACCACGCGCGGATCGTTCAGC
AGGAACTGCGCAAAGGCCTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSALQQEPHAGVEIDARGIATVTIREAGSLNILSTPVIASLTRAIEALAGNDAVRVLVLRGAGEKGFVGGADIKEMAALD
RASAEAFISGLRRLCDAVRHLPVPVIARMPGWCLGGGLELALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNA
RTAWMLLTGEITDAAEALQWGLVSRVVPLAELDLEIERVAALLAGFGPVAVRQQKRLLREWEDAPLEVSINNSVTEFGSA
FDTGEPQQHMATFLNRKR

Sequences:

>Translated_258_residues
MSALQQEPHAGVEIDARGIATVTIREAGSLNILSTPVIASLTRAIEALAGNDAVRVLVLRGAGEKGFVGGADIKEMAALD
RASAEAFISGLRRLCDAVRHLPVPVIARMPGWCLGGGLELALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNA
RTAWMLLTGEITDAAEALQWGLVSRVVPLAELDLEIERVAALLAGFGPVAVRQQKRLLREWEDAPLEVSINNSVTEFGSA
FDTGEPQQHMATFLNRKR
>Mature_257_residues
SALQQEPHAGVEIDARGIATVTIREAGSLNILSTPVIASLTRAIEALAGNDAVRVLVLRGAGEKGFVGGADIKEMAALDR
ASAEAFISGLRRLCDAVRHLPVPVIARMPGWCLGGGLELALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNAR
TAWMLLTGEITDAAEALQWGLVSRVVPLAELDLEIERVAALLAGFGPVAVRQQKRLLREWEDAPLEVSINNSVTEFGSAF
DTGEPQQHMATFLNRKR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=31.0204081632653, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI4502327, Length=209, Percent_Identity=36.8421052631579, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI31542718, Length=206, Percent_Identity=32.5242718446602, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI68989263, Length=159, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI70995211, Length=191, Percent_Identity=34.0314136125654, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI20127408, Length=198, Percent_Identity=31.8181818181818, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787659, Length=239, Percent_Identity=30.9623430962343, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI221142681, Length=175, Percent_Identity=37.7142857142857, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788682, Length=175, Percent_Identity=38.8571428571429, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1788597, Length=206, Percent_Identity=33.495145631068, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790281, Length=179, Percent_Identity=35.7541899441341, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082183, Length=249, Percent_Identity=25.3012048192771, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787660, Length=209, Percent_Identity=33.4928229665072, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=199, Percent_Identity=33.1658291457286, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=232, Percent_Identity=28.448275862069, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=243, Percent_Identity=28.8065843621399, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=167, Percent_Identity=31.7365269461078, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=232, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=193, Percent_Identity=30.5699481865285, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=193, Percent_Identity=30.5699481865285, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=193, Percent_Identity=30.5699481865285, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=204, Percent_Identity=23.0392156862745, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=183, Percent_Identity=28.4153005464481, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=247, Percent_Identity=23.8866396761134, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=27.8688524590164, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=27.8688524590164, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24653139, Length=247, Percent_Identity=29.9595141700405, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922422, Length=243, Percent_Identity=23.4567901234568, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=31.3186813186813, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=29.2452830188679, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19921000, Length=163, Percent_Identity=35.5828220858896, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650670, Length=244, Percent_Identity=28.2786885245902, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583077, Length=163, Percent_Identity=35.5828220858896, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24583079, Length=163, Percent_Identity=35.5828220858896, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27462; Mature: 27331

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALQQEPHAGVEIDARGIATVTIREAGSLNILSTPVIASLTRAIEALAGNDAVRVLVLR
CCCCCCCCCCCEEEECCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEEE
GAGEKGFVGGADIKEMAALDRASAEAFISGLRRLCDAVRHLPVPVIARMPGWCLGGGLEL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECCCCEE
ALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNARTAWMLLTGEITDAAEALQW
EEEEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
GLVSRVVPLAELDLEIERVAALLAGFGPVAVRQQKRLLREWEDAPLEVSINNSVTEFGSA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCC
FDTGEPQQHMATFLNRKR
CCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SALQQEPHAGVEIDARGIATVTIREAGSLNILSTPVIASLTRAIEALAGNDAVRVLVLR
CCCCCCCCCCEEEECCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEEE
GAGEKGFVGGADIKEMAALDRASAEAFISGLRRLCDAVRHLPVPVIARMPGWCLGGGLEL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECCCCEE
ALACDIRIAADNAQLGMPEVKVGIPSVIHAALMPRLIGNARTAWMLLTGEITDAAEALQW
EEEEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
GLVSRVVPLAELDLEIERVAALLAGFGPVAVRQQKRLLREWEDAPLEVSINNSVTEFGSA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCC
FDTGEPQQHMATFLNRKR
CCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA