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The map label for this gene is narH2 [H]

Identifier: 116696202

GI number: 116696202

Start: 2566723

End: 2568273

Strand: Direct

Name: narH2 [H]

Synonym: H16_B2266

Alternate gene names: 116696202

Gene position: 2566723-2568273 (Clockwise)

Preceding gene: 116696201

Following gene: 116696203

Centisome position: 88.13

GC content: 65.89

Gene sequence:

>1551_bases
ATGAAGATCCGTGCCCAGGTGGCCATGGTGCTGAACCTCGACAAGTGCATCGGCTGCCATACCTGCTCCGTGACCTGCAA
GAACGTCTGGACCAGCCGCGACGGTGTCGAGTACGCGTGGTTCAACAACGTCGAGACCAAGCCCGGCATCGGCTACCCCA
AGGAATGGGAGAACCAGGACAAGTGGCAGGGTGGCTGGAAGCGCAACGCCGACGGCACGCTGGAGCCGCGCCAGGGCGGC
AAGCTGAAGATCCTGGCGAACCTGTTCGCCAACCCCAACCTGCCGCAGATCGACGAGTACTACGAGCCCTTCACCTACGA
CTACGAGCACCTGCAGAAGGCGCCGCTGGTGCAGACCCCGCCCACCGCGCGCCCGGTCTCGGTGCTGACGGGCCGCAAGA
TGGAGAAGATCGAGTGGGGCCCGAACTGGGAAGACGACCTCGGCGGCGAGTTCAGCTCGCGCGGTCGCGACAAGCTCTTC
GACGACGTGCAGAAGGAGATGTACTCGACCTTCGAGAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAGCACTGCCT
GAACCCGGCCTGCGTGGCCTCGTGCCCGTCGGGCTCGATCTACAAGCGCGAGGACGACGGCATCGTGCTGGTCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCATCTCGGGCTGCCCGTACAAGAAGATCTACTTCAACTGGCAGAGCGGCAAGGCC
GAGAAGTGCCTGTTCTGCTATCCGCGCATCGAGGCCGGCCAGCCCACGGTCTGCTCCGAGACCTGCGTCGGCCGCATCCG
CTACCTCGGCGTGATGCTGTACGACGCCGACCGCATCGAGCAGGCGGCGTCGGTGGCCGATGAGCGCGACCTGTACCAGT
CGCAGCTCGACGTCTTCCTCGATCCGCACGACCCGGCGGTGCAGGCCGAGGCGCTGCGCCAGGGCATCCCGCAAACCTGG
CTCGATGCCGCGCGCAAGTCGCCGGTCTACAAGATGGCATGCGAGTGGAAGATCGCCTTCCCGCTGCACCCGGAGTACCG
CACCCTGCCGATGGTCTGGTATGTCCCGCCGCTGTCGCCGATCCAGTCCGCGGCCGAGGCCGGCCACATGGGCATGAACG
GCGTGATCCCGGACGTGAAGAGCCTGCGCATCCCGGTGAAGTACCTGGCCAACCTGCTGACCGCGGGCGACGTGATGCCG
GTACTGTCCGCGCTGGACCGCATGCTGGCGATGCGCGCCTACAAGCGCTCGCAGGTGGTGGACGGCGTGCAGGACCTGGG
CGTGCTGGAGCAGGTGGGCCTGACGCCCGCGCAGGTCGAGGACATGTACCAGACCATGGCCATCGCCAACTACGAGGACC
GCTTCGTGATCCCGTCCTCGCACAAGGAGATGGTCGAGGACAGCTTCAACGACAAGGCCAGCTGCGGCTTCACCTTCGGC
AACGGCTGCTCGGGCGGCGCCTCGGACGGCTCGCTGTTCGGCAAGAAGCCACAGGGCAGCGTCCACTTCGCCGACATGCC
CAAGTCGCGCAAGAAGGCAGTGATGAGCTGA

Upstream 100 bases:

>100_bases
TGGGCAGCAACCGCGACGAGTTCGTGATCCTGCGCAAGATGAAGAGGGTCGACTGGCTGGAAGGGCCGCTCGCGGAAAAG
ACTAAGGAAGGAGCCTCGCA

Downstream 100 bases:

>100_bases
TGCCTGCCACGAACCGCACGGAGAACAACATGCCCCTCTATCCCATCCTCAGCGCCCTGCTCGGCTATCCCGAGCAGGAA
CTGCTCGACGCCCTGCCCGA

Product: respiratory nitrate reductase beta chain (NarH)

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNADGTLEPRQGG
KLKILANLFANPNLPQIDEYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DDVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPAVQAEALRQGIPQTW
LDAARKSPVYKMACEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
NGCSGGASDGSLFGKKPQGSVHFADMPKSRKKAVMS

Sequences:

>Translated_516_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNADGTLEPRQGG
KLKILANLFANPNLPQIDEYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DDVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPAVQAEALRQGIPQTW
LDAARKSPVYKMACEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
NGCSGGASDGSLFGKKPQGSVHFADMPKSRKKAVMS
>Mature_516_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQDKWQGGWKRNADGTLEPRQGG
KLKILANLFANPNLPQIDEYYEPFTYDYEHLQKAPLVQTPPTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLF
DDVQKEMYSTFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFLDPHDPAVQAEALRQGIPQTW
LDAARKSPVYKMACEWKIAFPLHPEYRTLPMVWYVPPLSPIQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMP
VLSALDRMLAMRAYKRSQVVDGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
NGCSGGASDGSLFGKKPQGSVHFADMPKSRKKAVMS

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=497, Percent_Identity=71.2273641851107, Blast_Score=781, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=507, Percent_Identity=69.0335305719921, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=33.9506172839506, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787122, Length=99, Percent_Identity=43.4343434343434, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787749, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=31.6831683168317, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=35.5555555555556, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=35.0515463917526, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58064; Mature: 58064

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNADGTLEPRQGGKLKILANLFANPNLPQIDEYYEPFTYDYEHLQKAPLVQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLFDDVQKEMYSTFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPAVQAEALRQGIPQTWLDAARKSPVYKMACEWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMPVLSALDRMLAMRAYKRSQVV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCEEEC
NGCSGGASDGSLFGKKPQGSVHFADMPKSRKKAVMS
CCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCC
>Mature Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGIGYPKEWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNADGTLEPRQGGKLKILANLFANPNLPQIDEYYEPFTYDYEHLQKAPLVQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCHHHHHCCCCCCHHHHCCCCCCCCC
PTARPVSVLTGRKMEKIEWGPNWEDDLGGEFSSRGRDKLFDDVQKEMYSTFENTFMMYLP
CCCCCHHHHCCCCHHCCCCCCCCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQSGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCLFCYPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADERDLYQSQLDVFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHEEE
DPHDPAVQAEALRQGIPQTWLDAARKSPVYKMACEWKIAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEEECCCCHH
IQSAAEAGHMGMNGVIPDVKSLRIPVKYLANLLTAGDVMPVLSALDRMLAMRAYKRSQVV
HHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DGVQDLGVLEQVGLTPAQVEDMYQTMAIANYEDRFVIPSSHKEMVEDSFNDKASCGFTFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCEEEC
NGCSGGASDGSLFGKKPQGSVHFADMPKSRKKAVMS
CCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]