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The map label for this gene is bkdB [H]

Identifier: 116696171

GI number: 116696171

Start: 2534260

End: 2535378

Strand: Direct

Name: bkdB [H]

Synonym: H16_B2235

Alternate gene names: 116696171

Gene position: 2534260-2535378 (Clockwise)

Preceding gene: 116696170

Following gene: 116696172

Centisome position: 87.01

GC content: 72.03

Gene sequence:

>1119_bases
ATGAGAATCTTCAAGCTGCCCGACCTGGGCGAAGGCCTGCAGGAGGCCGAGATCGTGACCTGGCACGTCAAGACCGGCGA
CACCGTGGCCGCTGACCAGCCGCTGCTGTCGGTGGAGACGGCCAAGGCCATCGTGGAAATCCCGTCGCCCTATGCAGGCA
CCATCGCCAAGCTGTTTGCGCAGCCCGGCGATATCGTCCACCTGGGCGCGCCGCTGGTCGGCGTCGAGGGTGCGGGCGAG
GATGCCGACGCCGGCACCGTGGTGGGCTCGGTCCAGGTCGGCACGCACGTGGTCAATGAAGCCGCGCCCGCGGGCTCCGC
GGCACCCGCCGCGGCCATGGCCGCCCGCGTCAAGGCCACGCCGGCGGTGCGCGCGCTGGCGCGCCGGCTCGGGGTGGACC
TGGCAATGGCCACGGCATCGGGCCCCGAGGGCGTCGTCACCGCCGCCGACGTGGAGCGGGTAGCCAGCACGCTGGCCGAA
CTGGGCACGCCGGAACAGCTGCGCGGCGTGCGCCGGGCGATGGCGCAGAACATGGCGCGTGCACAAGCCGAAGTGGCCGC
CGCCACCGTGATGGACGACGCCGACATCCACGCCTGGCAGCCCGGCGCCGATGTCACCATCCGGCTGGTGCGCGCCCTGG
TGGCCGGCTGCCGCGCCGAACCCGGCCTCAATGCCTGGTACGAAGGCCAGACCGCCCGCCGCCACGTACTGAAGAAGATC
GACGTCGGCATCGCGGCCGACCTGCCCGAAGGCCTGTTCGTGCCGGTGCTGCGCGACGTCGGCAACCGCGATGCCGCAGA
CCTGCGCCACGGCCTGGACCGCATGCGCGCCGACATCCGCGCGCGCACCATCGCGCCGGAGGAGATGCGCGGCAACACCA
TCACGCTGTCCAACTTCGGCATGATCGCGGGGCGCTATGCCGCGCCAATCGTGGTGCCGCCGACCGTGGCAATCCTGGGT
GCGGGGCGCGTGCGCGAGGAAGTGGTAGCAGCCGGCGGCGTGCCGGCGGTGCACCGGGTGATGCCGCTGAGCCTGACCTT
TGACCATCGCGTGGTGACGGGTGGGGAGGCGGCGCGGTTTCTGGCGGCGGTGATTGCGGATCTGGAGATGGCGGTGTAG

Upstream 100 bases:

>100_bases
GAATACACCTACCTGCCTGGCGTCGCCCGCATCGTCGACGCGGCGCGCAAGGCGATGGCAGCGTAATGCGAGAGTTGCCG
ACGATAGGCAAGGAGTGCCG

Downstream 100 bases:

>100_bases
CGGTCGAAGACAACAGGGAGCCGACGAGCAAATAGCCTCGGAGAATACTGCCGCCCCTCGTGTACGCTCCCCTCTCCCGC
AAGCGGAAGAGGGGAGCAAA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRIFKLPDLGEGLQEAEIVTWHVKTGDTVAADQPLLSVETAKAIVEIPSPYAGTIAKLFAQPGDIVHLGAPLVGVEGAGE
DADAGTVVGSVQVGTHVVNEAAPAGSAAPAAAMAARVKATPAVRALARRLGVDLAMATASGPEGVVTAADVERVASTLAE
LGTPEQLRGVRRAMAQNMARAQAEVAAATVMDDADIHAWQPGADVTIRLVRALVAGCRAEPGLNAWYEGQTARRHVLKKI
DVGIAADLPEGLFVPVLRDVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPTVAILG
AGRVREEVVAAGGVPAVHRVMPLSLTFDHRVVTGGEAARFLAAVIADLEMAV

Sequences:

>Translated_372_residues
MRIFKLPDLGEGLQEAEIVTWHVKTGDTVAADQPLLSVETAKAIVEIPSPYAGTIAKLFAQPGDIVHLGAPLVGVEGAGE
DADAGTVVGSVQVGTHVVNEAAPAGSAAPAAAMAARVKATPAVRALARRLGVDLAMATASGPEGVVTAADVERVASTLAE
LGTPEQLRGVRRAMAQNMARAQAEVAAATVMDDADIHAWQPGADVTIRLVRALVAGCRAEPGLNAWYEGQTARRHVLKKI
DVGIAADLPEGLFVPVLRDVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPTVAILG
AGRVREEVVAAGGVPAVHRVMPLSLTFDHRVVTGGEAARFLAAVIADLEMAV
>Mature_372_residues
MRIFKLPDLGEGLQEAEIVTWHVKTGDTVAADQPLLSVETAKAIVEIPSPYAGTIAKLFAQPGDIVHLGAPLVGVEGAGE
DADAGTVVGSVQVGTHVVNEAAPAGSAAPAAAMAARVKATPAVRALARRLGVDLAMATASGPEGVVTAADVERVASTLAE
LGTPEQLRGVRRAMAQNMARAQAEVAAATVMDDADIHAWQPGADVTIRLVRALVAGCRAEPGLNAWYEGQTARRHVLKKI
DVGIAADLPEGLFVPVLRDVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPTVAILG
AGRVREEVVAAGGVPAVHRVMPLSLTFDHRVVTGGEAARFLAAVIADLEMAV

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=410, Percent_Identity=27.0731707317073, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI19923748, Length=220, Percent_Identity=29.5454545454545, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=21.1453744493392, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=21.3656387665198, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI31711992, Length=424, Percent_Identity=23.1132075471698, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786305, Length=410, Percent_Identity=28.5365853658537, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1786946, Length=413, Percent_Identity=25.181598062954, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17537937, Length=410, Percent_Identity=27.3170731707317, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI25146366, Length=400, Percent_Identity=29, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=391, Percent_Identity=26.5984654731458, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324258, Length=172, Percent_Identity=30.8139534883721, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18859875, Length=413, Percent_Identity=25.90799031477, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24645909, Length=130, Percent_Identity=36.1538461538462, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24582497, Length=168, Percent_Identity=26.7857142857143, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129315, Length=168, Percent_Identity=26.7857142857143, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 38613; Mature: 38613

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIFKLPDLGEGLQEAEIVTWHVKTGDTVAADQPLLSVETAKAIVEIPSPYAGTIAKLFA
CCEECCCCCCCCCCCCEEEEEEEECCCEECCCCCCHHHHHHHHHEECCCCHHHHHHHHHC
QPGDIVHLGAPLVGVEGAGEDADAGTVVGSVQVGTHVVNEAAPAGSAAPAAAMAARVKAT
CCCCEEEECCCEEECCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
PAVRALARRLGVDLAMATASGPEGVVTAADVERVASTLAELGTPEQLRGVRRAMAQNMAR
HHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AQAEVAAATVMDDADIHAWQPGADVTIRLVRALVAGCRAEPGLNAWYEGQTARRHVLKKI
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
DVGIAADLPEGLFVPVLRDVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFG
CCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCC
MIAGRYAAPIVVPPTVAILGAGRVREEVVAAGGVPAVHRVMPLSLTFDHRVVTGGEAARF
CCHHCCCCCEEECCHHEEECCCHHHHHHHHCCCCCHHHHHEEEEEEECCEEEECCHHHHH
LAAVIADLEMAV
HHHHHHHHHHCC
>Mature Secondary Structure
MRIFKLPDLGEGLQEAEIVTWHVKTGDTVAADQPLLSVETAKAIVEIPSPYAGTIAKLFA
CCEECCCCCCCCCCCCEEEEEEEECCCEECCCCCCHHHHHHHHHEECCCCHHHHHHHHHC
QPGDIVHLGAPLVGVEGAGEDADAGTVVGSVQVGTHVVNEAAPAGSAAPAAAMAARVKAT
CCCCEEEECCCEEECCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
PAVRALARRLGVDLAMATASGPEGVVTAADVERVASTLAELGTPEQLRGVRRAMAQNMAR
HHHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AQAEVAAATVMDDADIHAWQPGADVTIRLVRALVAGCRAEPGLNAWYEGQTARRHVLKKI
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
DVGIAADLPEGLFVPVLRDVGNRDAADLRHGLDRMRADIRARTIAPEEMRGNTITLSNFG
CCCEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEECCC
MIAGRYAAPIVVPPTVAILGAGRVREEVVAAGGVPAVHRVMPLSLTFDHRVVTGGEAARF
CCHHCCCCCEEECCHHEEECCCHHHHHHHHCCCCCHHHHHEEEEEEECCEEEECCHHHHH
LAAVIADLEMAV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]