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The map label for this gene is bkdA2 [H]

Identifier: 116696170

GI number: 116696170

Start: 2533248

End: 2534225

Strand: Direct

Name: bkdA2 [H]

Synonym: H16_B2234

Alternate gene names: 116696170

Gene position: 2533248-2534225 (Clockwise)

Preceding gene: 116696169

Following gene: 116696171

Centisome position: 86.98

GC content: 69.84

Gene sequence:

>978_bases
ATGGCGGAAATCAATCTGGTCGAAGCAGTCAACCTGGCGCTGGCCCATGCGCTCGAGCACGATCCCGATGTGCTGCTGCT
GGGCGAGGATATCGGCGTCAATGGCGGCGTGTTCCGCGCCACCGCGGGCCTGCAGGCGCGCTTTGGCGCGGCGCGCGTCA
TGGATACGCCGCTGGCCGAAGGCGGCATCGTCGGCGCGGCGATCGGCATGGCGGCAATGGGGCTCAAGCCGGTGGCCGAG
ATCCAGTTCACCGGCTTTATCTACCCCGCGGTCGACCACATCATCAACCACGCCGCACGCATGCGGCATCGCACGCGCGG
GCGCTTGTCATGTCCGATGGTGGTGCGCTCGCCGTGCGGTGCCGGCATCCATGCACCCGAGCATCATTCCGAAAGCCCGG
AGGCGATGTTCGCGCATATGCCCGGCATCCGCGTGGTGGTGCCTTCATCGCCCGCGCGCGCGTACGGGCTGCTGCTGGCG
GCCATCGCCGATCCGGACCCGGTCATCTTCCTCGAACCCACGCGGCTGTACCGCCTGTTCCGCCAGGAAGTGGCCGACGA
CGGCGCCGCGCTGCCGCTGGACACCTGCTTCACGCTGCGCGAAGGCAGCGACATCACGCTGGTGAGCTGGGGCGCGATGG
TGCAGGAGACGCTCGCCGCCGCGGACGCACTGGCAGGCGAAGGCGTGACGGCCACGGTCATCGACGTGGCCACGCTCAAG
CCGCTGGACATGCAGACCATCCTGGAATCGGTGACGCGCACGGGCCGCTGCGTGATCGTGCACGAGGCCCCGCGCACCGC
CGGCTTCGGCGCCGAGATCGCGGCGCAGCTGGCCGACGCGGGCCTCTACTCACTGGCCGCGCCGGTGCAGCGCGTGACCG
GCTTCGACACCGTGGTGCCGCTGGCGCGGCTGGAATACACCTACCTGCCTGGCGTCGCCCGCATCGTCGACGCGGCGCGC
AAGGCGATGGCAGCGTAA

Upstream 100 bases:

>100_bases
CGAATTGCAGGCGCAGCTGGAGATTGCCCGGCGCTTCCTGGCGCCGCACGGATAACCAGCAAACCCTGCGCCTTCACCTG
GCGACCACAGCGAGCGAACC

Downstream 100 bases:

>100_bases
TGCGAGAGTTGCCGACGATAGGCAAGGAGTGCCGATGAGAATCTTCAAGCTGCCCGACCTGGGCGAAGGCCTGCAGGAGG
CCGAGATCGTGACCTGGCAC

Product: 2-oxoisovalerate dehydrogenase E1 component subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MAEINLVEAVNLALAHALEHDPDVLLLGEDIGVNGGVFRATAGLQARFGAARVMDTPLAEGGIVGAAIGMAAMGLKPVAE
IQFTGFIYPAVDHIINHAARMRHRTRGRLSCPMVVRSPCGAGIHAPEHHSESPEAMFAHMPGIRVVVPSSPARAYGLLLA
AIADPDPVIFLEPTRLYRLFRQEVADDGAALPLDTCFTLREGSDITLVSWGAMVQETLAAADALAGEGVTATVIDVATLK
PLDMQTILESVTRTGRCVIVHEAPRTAGFGAEIAAQLADAGLYSLAAPVQRVTGFDTVVPLARLEYTYLPGVARIVDAAR
KAMAA

Sequences:

>Translated_325_residues
MAEINLVEAVNLALAHALEHDPDVLLLGEDIGVNGGVFRATAGLQARFGAARVMDTPLAEGGIVGAAIGMAAMGLKPVAE
IQFTGFIYPAVDHIINHAARMRHRTRGRLSCPMVVRSPCGAGIHAPEHHSESPEAMFAHMPGIRVVVPSSPARAYGLLLA
AIADPDPVIFLEPTRLYRLFRQEVADDGAALPLDTCFTLREGSDITLVSWGAMVQETLAAADALAGEGVTATVIDVATLK
PLDMQTILESVTRTGRCVIVHEAPRTAGFGAEIAAQLADAGLYSLAAPVQRVTGFDTVVPLARLEYTYLPGVARIVDAAR
KAMAA
>Mature_324_residues
AEINLVEAVNLALAHALEHDPDVLLLGEDIGVNGGVFRATAGLQARFGAARVMDTPLAEGGIVGAAIGMAAMGLKPVAEI
QFTGFIYPAVDHIINHAARMRHRTRGRLSCPMVVRSPCGAGIHAPEHHSESPEAMFAHMPGIRVVVPSSPARAYGLLLAA
IADPDPVIFLEPTRLYRLFRQEVADDGAALPLDTCFTLREGSDITLVSWGAMVQETLAAADALAGEGVTATVIDVATLKP
LDMQTILESVTRTGRCVIVHEAPRTAGFGAEIAAQLADAGLYSLAAPVQRVTGFDTVVPLARLEYTYLPGVARIVDAARK
AMAA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=324, Percent_Identity=44.7530864197531, Blast_Score=277, Evalue=9e-75,
Organism=Homo sapiens, GI34101272, Length=324, Percent_Identity=44.7530864197531, Blast_Score=277, Evalue=9e-75,
Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=35.2760736196319, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI291084858, Length=326, Percent_Identity=33.7423312883436, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI225637461, Length=251, Percent_Identity=26.6932270916335, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI225637463, Length=251, Percent_Identity=26.6932270916335, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI225637459, Length=251, Percent_Identity=26.6932270916335, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17506935, Length=324, Percent_Identity=45.3703703703704, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17538422, Length=321, Percent_Identity=38.006230529595, Blast_Score=207, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=38.719512195122, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI160714828, Length=317, Percent_Identity=43.8485804416404, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI160714832, Length=317, Percent_Identity=43.8485804416404, Blast_Score=265, Evalue=4e-71,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=38.3435582822086, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=38.3435582822086, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=37.3626373626374, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=37.3626373626374, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 34210; Mature: 34079

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEINLVEAVNLALAHALEHDPDVLLLGEDIGVNGGVFRATAGLQARFGAARVMDTPLAE
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCHHHCCCHHHHCCCCCC
GGIVGAAIGMAAMGLKPVAEIQFTGFIYPAVDHIINHAARMRHRTRGRLSCPMVVRSPCG
CCHHHHHHHHHHHCCCCHHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
AGIHAPEHHSESPEAMFAHMPGIRVVVPSSPARAYGLLLAAIADPDPVIFLEPTRLYRLF
CCCCCCCCCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHEECCCCCEEEECHHHHHHHH
RQEVADDGAALPLDTCFTLREGSDITLVSWGAMVQETLAAADALAGEGVTATVIDVATLK
HHHHCCCCCCCCHHHEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCC
PLDMQTILESVTRTGRCVIVHEAPRTAGFGAEIAAQLADAGLYSLAAPVQRVTGFDTVVP
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LARLEYTYLPGVARIVDAARKAMAA
HHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEINLVEAVNLALAHALEHDPDVLLLGEDIGVNGGVFRATAGLQARFGAARVMDTPLAE
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCHHHCCCHHHHCCCCCC
GGIVGAAIGMAAMGLKPVAEIQFTGFIYPAVDHIINHAARMRHRTRGRLSCPMVVRSPCG
CCHHHHHHHHHHHCCCCHHEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
AGIHAPEHHSESPEAMFAHMPGIRVVVPSSPARAYGLLLAAIADPDPVIFLEPTRLYRLF
CCCCCCCCCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHEECCCCCEEEECHHHHHHHH
RQEVADDGAALPLDTCFTLREGSDITLVSWGAMVQETLAAADALAGEGVTATVIDVATLK
HHHHCCCCCCCCHHHEEEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEHHHCC
PLDMQTILESVTRTGRCVIVHEAPRTAGFGAEIAAQLADAGLYSLAAPVQRVTGFDTVVP
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LARLEYTYLPGVARIVDAARKAMAA
HHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629 [H]