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The map label for this gene is nac [H]

Identifier: 116696051

GI number: 116696051

Start: 2390129

End: 2391106

Strand: Direct

Name: nac [H]

Synonym: H16_B2115

Alternate gene names: 116696051

Gene position: 2390129-2391106 (Clockwise)

Preceding gene: 116696048

Following gene: 116696052

Centisome position: 82.06

GC content: 69.63

Gene sequence:

>978_bases
ATGGACCTGCGCGCCATGCGCTATTTCATCGCCATCGTCGACCAGGGCAGCCTGACGCGGGCAGCCGAAGTGGTCTGCGT
GGCGCAGCCCGCACTCAGCCAGCAGCTGGCGGCGCTGGAAAAAGAGCTGGGCGTGCCGCTGGTCCATCGCGGCGCCAAGG
GCGTCAAACCCACCGAAGCCGGCCGCACACTCTATCGCCACGCCCGCAATATCCTGCGGCAGGTGGAGATCGCCGGCGCC
GAGGCCCGGGTGGCCGGCACGGATGTTACCGGCATGGTTGCCATCGGCCTGCCCACCACGGCGGCAGCGGCATTCGGCAT
GCCGCTGATCCGCGCGGTGCGCGCCCGCTATCCGCAAATCCGGCTGCAGCTGTTCGAGAGCATGAGCGGTTATATCTCGG
AGCTGCTGGACCACAACCGGCTGGACTTCGCCATCCTGTTCCGCGATGTGCCGTCGCGCGCGGTGGAACTGGAGCGGCTG
GCAAGCGAATCGCTATACCTAGTCGGGACGCTTCCCGTGACGCGCGGGCGCGCAAAGGCGCAAGCGAAGCCTCGGGCGCT
GGCCGGTGCGGAAGGCGCTACGGTCGCGATCAAGGCACTGGGCGAGCTGCCACTCGTGCTGCCAAGCGGCTCGCAGGGCC
TGCGTGAAGTGGTCGAACTTGCCTTCGCGCAGGCCGGCGTGTCGCTGAACGTGGTGGCCGACCTGGATTCGCTGCCCTTC
CTGCTGGCGACCGCGCGCGAGGGCCTGGCCTGCACCATCCTGCCCGCGTCGTCGCTGTCCGACGCGGACAGCGAAGTGCC
GCGGCACCTGATCGTGCCGGAACTGCGCCGCACGCTGTCGCTGTGCTGGCCACGCGCGCTGCCGCGCACCAGCGCGGCCG
AGGCCGTGGCGGAGATGCTGCGTGAGCTGGTGGCCGAGCGCATTGCGGCGGGCGGGTCGCTCAAGCCGCTCATTTCGCCG
CCTGCGGCCGATCAATAA

Upstream 100 bases:

>100_bases
CCTGACGGCAATCTACCGGAGCGGGCCGCACCTGGGAAATACCGATTGCCGAGGGGATCATAAGTAAAATGAATACTTTG
GAATTCTCCCGGAGCCTGCC

Downstream 100 bases:

>100_bases
AACTTATGCCCGTAGTCGGCGATTGAATTTCACGCCGGCGCGGCAGTCGGCTAGAGTTGCCACCAGCCTGGGCCGGCATT
CACGGCCCCGCAATTCCAGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MDLRAMRYFIAIVDQGSLTRAAEVVCVAQPALSQQLAALEKELGVPLVHRGAKGVKPTEAGRTLYRHARNILRQVEIAGA
EARVAGTDVTGMVAIGLPTTAAAAFGMPLIRAVRARYPQIRLQLFESMSGYISELLDHNRLDFAILFRDVPSRAVELERL
ASESLYLVGTLPVTRGRAKAQAKPRALAGAEGATVAIKALGELPLVLPSGSQGLREVVELAFAQAGVSLNVVADLDSLPF
LLATAREGLACTILPASSLSDADSEVPRHLIVPELRRTLSLCWPRALPRTSAAEAVAEMLRELVAERIAAGGSLKPLISP
PAADQ

Sequences:

>Translated_325_residues
MDLRAMRYFIAIVDQGSLTRAAEVVCVAQPALSQQLAALEKELGVPLVHRGAKGVKPTEAGRTLYRHARNILRQVEIAGA
EARVAGTDVTGMVAIGLPTTAAAAFGMPLIRAVRARYPQIRLQLFESMSGYISELLDHNRLDFAILFRDVPSRAVELERL
ASESLYLVGTLPVTRGRAKAQAKPRALAGAEGATVAIKALGELPLVLPSGSQGLREVVELAFAQAGVSLNVVADLDSLPF
LLATAREGLACTILPASSLSDADSEVPRHLIVPELRRTLSLCWPRALPRTSAAEAVAEMLRELVAERIAAGGSLKPLISP
PAADQ
>Mature_325_residues
MDLRAMRYFIAIVDQGSLTRAAEVVCVAQPALSQQLAALEKELGVPLVHRGAKGVKPTEAGRTLYRHARNILRQVEIAGA
EARVAGTDVTGMVAIGLPTTAAAAFGMPLIRAVRARYPQIRLQLFESMSGYISELLDHNRLDFAILFRDVPSRAVELERL
ASESLYLVGTLPVTRGRAKAQAKPRALAGAEGATVAIKALGELPLVLPSGSQGLREVVELAFAQAGVSLNVVADLDSLPF
LLATAREGLACTILPASSLSDADSEVPRHLIVPELRRTLSLCWPRALPRTSAAEAVAEMLRELVAERIAAGGSLKPLISP
PAADQ

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=309, Percent_Identity=37.2168284789644, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI157672245, Length=297, Percent_Identity=34.3434343434343, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1790399, Length=304, Percent_Identity=29.2763157894737, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788748, Length=301, Percent_Identity=24.9169435215947, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693105, Length=283, Percent_Identity=25.4416961130742, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786401, Length=139, Percent_Identity=35.9712230215827, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788887, Length=256, Percent_Identity=28.515625, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=28.2758620689655, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=133, Percent_Identity=35.3383458646617, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787530, Length=264, Percent_Identity=26.5151515151515, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789506, Length=302, Percent_Identity=22.8476821192053, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788626, Length=261, Percent_Identity=24.5210727969349, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=125, Percent_Identity=29.6, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=181, Percent_Identity=32.5966850828729, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367136, Length=144, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34569; Mature: 34569

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRAMRYFIAIVDQGSLTRAAEVVCVAQPALSQQLAALEKELGVPLVHRGAKGVKPTEA
CCHHHHEHHHHEECCCCCHHHHHEEEECCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
GRTLYRHARNILRQVEIAGAEARVAGTDVTGMVAIGLPTTAAAAFGMPLIRAVRARYPQI
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHH
RLQLFESMSGYISELLDHNRLDFAILFRDVPSRAVELERLASESLYLVGTLPVTRGRAKA
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCHHH
QAKPRALAGAEGATVAIKALGELPLVLPSGSQGLREVVELAFAQAGVSLNVVADLDSLPF
CCCCCHHCCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHH
LLATAREGLACTILPASSLSDADSEVPRHLIVPELRRTLSLCWPRALPRTSAAEAVAEML
HHHHHCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RELVAERIAAGGSLKPLISPPAADQ
HHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDLRAMRYFIAIVDQGSLTRAAEVVCVAQPALSQQLAALEKELGVPLVHRGAKGVKPTEA
CCHHHHEHHHHEECCCCCHHHHHEEEECCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
GRTLYRHARNILRQVEIAGAEARVAGTDVTGMVAIGLPTTAAAAFGMPLIRAVRARYPQI
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCHHH
RLQLFESMSGYISELLDHNRLDFAILFRDVPSRAVELERLASESLYLVGTLPVTRGRAKA
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCHHH
QAKPRALAGAEGATVAIKALGELPLVLPSGSQGLREVVELAFAQAGVSLNVVADLDSLPF
CCCCCHHCCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHH
LLATAREGLACTILPASSLSDADSEVPRHLIVPELRRTLSLCWPRALPRTSAAEAVAEML
HHHHHCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RELVAERIAAGGSLKPLISPPAADQ
HHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]