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The map label for this gene is citH [H]

Identifier: 116696048

GI number: 116696048

Start: 2387415

End: 2387987

Strand: Direct

Name: citH [H]

Synonym: H16_B2112

Alternate gene names: 116696048

Gene position: 2387415-2387987 (Clockwise)

Preceding gene: 116696046

Following gene: 116696051

Centisome position: 81.97

GC content: 68.59

Gene sequence:

>573_bases
GTGGTGGTGGCCGGCGTGCTGGTGACGATCGGCGGCACCTCCGCCACCTATATCGTGCTGCACTACATGACCAGCTACGC
GGTGACGGTACTGAAGATCCCGCTCGGGCTCAGCATGGCCGCCGGCTGCGTCGCCGCACTGGTGCAGGTGGCCCTGTCTG
CGTATGCCGGCAGGCTGTCCGACCGCATCGGCCGCAAGCCCGCCATCCTGTGGTCGCGCGTGGCGATGCTGGTGCTGGTC
TACCCCGCCTTCCTGCTGCTCAACGCGCGGCCGTCGCTCGGCACGCTGCTGGCCGTGGTCGCGATGCTGGCCCTGCCGCT
GGTGCTGAACGTAGTGCCGTCCGTGGTGCTGATCACCGAGCTGTTCCCGCGCCGCATTCGCGCGAGCGGGCTGTCGGTGG
TCTATTGCATCGGCGTGCTGGTGTTCGGCGGCTTCGCGCAGCTGATCGCCACCTGGCTGATCGAACTGACCGGCAATGCC
AGCGCCCCTGCGCTATATGTGATCGGCTGCGGCCTGATTTCGCTGGTCGGGCTGGCGATGGCGCCGGAGACGGCGGGCAA
GCGGCTGGACTGA

Upstream 100 bases:

>100_bases
TATCCGCCGCCACCTGGAAGAAACCGCCGAAGCGCCGGCCGCCGGCGCGCCGGGCCATGAACGCGCAGGCCTGACTGAAA
TCTTCCGTGGGCACGGCCGC

Downstream 100 bases:

>100_bases
CTGTGGCAGACTACCCACCGGACAAGATCCGGCGCGCGCGCTCCAGCACCGGGCGGTCCACCATCTTGCCGTCCACCGCC
ACCGCGGCGCCATCGGCAGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MVVAGVLVTIGGTSATYIVLHYMTSYAVTVLKIPLGLSMAAGCVAALVQVALSAYAGRLSDRIGRKPAILWSRVAMLVLV
YPAFLLLNARPSLGTLLAVVAMLALPLVLNVVPSVVLITELFPRRIRASGLSVVYCIGVLVFGGFAQLIATWLIELTGNA
SAPALYVIGCGLISLVGLAMAPETAGKRLD

Sequences:

>Translated_190_residues
MVVAGVLVTIGGTSATYIVLHYMTSYAVTVLKIPLGLSMAAGCVAALVQVALSAYAGRLSDRIGRKPAILWSRVAMLVLV
YPAFLLLNARPSLGTLLAVVAMLALPLVLNVVPSVVLITELFPRRIRASGLSVVYCIGVLVFGGFAQLIATWLIELTGNA
SAPALYVIGCGLISLVGLAMAPETAGKRLD
>Mature_190_residues
MVVAGVLVTIGGTSATYIVLHYMTSYAVTVLKIPLGLSMAAGCVAALVQVALSAYAGRLSDRIGRKPAILWSRVAMLVLV
YPAFLLLNARPSLGTLLAVVAMLALPLVLNVVPSVVLITELFPRRIRASGLSVVYCIGVLVFGGFAQLIATWLIELTGNA
SAPALYVIGCGLISLVGLAMAPETAGKRLD

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=179, Percent_Identity=34.6368715083799, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 19779; Mature: 19779

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVAGVLVTIGGTSATYIVLHYMTSYAVTVLKIPLGLSMAAGCVAALVQVALSAYAGRLS
CEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DRIGRKPAILWSRVAMLVLVYPAFLLLNARPSLGTLLAVVAMLALPLVLNVVPSVVLITE
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPRRIRASGLSVVYCIGVLVFGGFAQLIATWLIELTGNASAPALYVIGCGLISLVGLAM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
APETAGKRLD
CCCCCCCCCC
>Mature Secondary Structure
MVVAGVLVTIGGTSATYIVLHYMTSYAVTVLKIPLGLSMAAGCVAALVQVALSAYAGRLS
CEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DRIGRKPAILWSRVAMLVLVYPAFLLLNARPSLGTLLAVVAMLALPLVLNVVPSVVLITE
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPRRIRASGLSVVYCIGVLVFGGFAQLIATWLIELTGNASAPALYVIGCGLISLVGLAM
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
APETAGKRLD
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]