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The map label for this gene is shiA [H]

Identifier: 116696003

GI number: 116696003

Start: 2323872

End: 2325182

Strand: Direct

Name: shiA [H]

Synonym: H16_B2067

Alternate gene names: 116696003

Gene position: 2323872-2325182 (Clockwise)

Preceding gene: 116696001

Following gene: 116696004

Centisome position: 79.79

GC content: 68.73

Gene sequence:

>1311_bases
ATGCAAGTGTCCCCTTCCGGCACGCGCCAGCCCGTGCGCGCCGCCACCGCCGCCTTTGTCGGCACCATGATCGAGTGGTA
CGACTTCTACATCTACGCCACCGCCGCCGCGCTGGTGTTCGGCCCGTTGTTCTTTCCCGGCGGCGATCCCTTCCTGAGCA
CGCTGGCGTCATTCGGCACCTTCGCGGTCGGCTTCTTTGCCCGTCCGCTCGGCGGCATCGTGTTCGGCCACTGGGGCGAC
CGCATCGGCCGCAAGCAGGCGCTGATGATCACGCTCATGATGATGGGCGTGGCCACCATCGGCATCGGCCTGCTGCCGAC
CTACGCCACTGCGGGCGCGATCGCGCCGGTGCTGCTGGTCCTGCTGCGCGTGGTGCAGGGCATCGCCGTGGGCGGCGAAT
GGGGCGGCGCGGTGCTGATGGCGGGCGAGCATGCCCCCAAGGGCCGCCGCACCTTCTTCGCCTCGTTCGCGCAGCTCGGC
AGCCCGGCCGGCCTGATCCTGTCGCTGCTGGCTTTCCGCGCGGTCACGTCGATGGAGCACGAGGTGTTCATGGACTGGGG
CTGGCGCCTGCCATTCCTGGCCAGCGCGGCGCTGCTGCTGGTGGGCGTGGCGATCCGGCTGGGCGTGAATGAATCGCCCG
AGTTCGCGCAGGTGCAGGCGCAGCGCAAGACCGCCAGGCTGCCGCTTGCCGAGGTCTGGCGCACGGCCCGCGTGCCGGTG
CTGCTGTGCATCGGCGCCAATACCATCGGCATTGCGGGGGTGTACTTCACCAACACCTTCATGATTGCCTACACCACGCA
ATACCTGTCGCTGCCCAGGACGCTGATCCTCGACTGCCTGTTCGCCGTCGCCATCATCCAGTTCCTGGCGCAGCCGCTGG
CCGCCTGGCTGGCAGAGAAGCTTGGCGGCGCGCGCTTCCTCAAGCTGGCCGCGCTGCTGGCGATGGCTTCACCCTATCCG
ATGTTCCTGCTGGTGCAGACGCGCCAGGCGCCGGCCATCGTGCTCGGCATCGCGCTGGCGGTGGTGTGCATGGCGAGCTT
CTATTCGGTGATCGCGGGCTTTGTCAGCGGTGTCTTCGAAACGCGCGTGCGCTACTCGGCGATCTCGTTGTCCTACCAGG
TCTGCGGCGCGGTGGCGGGCGGGCTGACACCGCTGGTGGGGACCGTCCTCGCGCAGCGCTTCCAGGGCCAATGGTGGCCG
CTGGCGGTGTTCTACACCTTGCTGGCCGGCGTGTCGCTGCTGTGCATCGCCAGCATCGATGCACGCCAGCGCCAGCAACC
ACAACCGGCCGCGCAAGCCGCGCAGGCCTGA

Upstream 100 bases:

>100_bases
AAATAAATATTAGAAATTTGACTGATATTTGTCAACGCGCGATAACTCCTCCCACAGCTGCCCCTGATGCAGCGGCACAA
ACATAGTGAGGAGCCAGTCC

Downstream 100 bases:

>100_bases
CGCCTTTCCACGGACATTGCAAACCCCATGCTGACGTTTTTCCATCCCGAGCAATTGCTGCACCATCCGCAGAGCTACTA
CTCGCGCGGCAGGATGCGCT

Product: major facilitator superfamily transporter MHS family protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MQVSPSGTRQPVRAATAAFVGTMIEWYDFYIYATAAALVFGPLFFPGGDPFLSTLASFGTFAVGFFARPLGGIVFGHWGD
RIGRKQALMITLMMMGVATIGIGLLPTYATAGAIAPVLLVLLRVVQGIAVGGEWGGAVLMAGEHAPKGRRTFFASFAQLG
SPAGLILSLLAFRAVTSMEHEVFMDWGWRLPFLASAALLLVGVAIRLGVNESPEFAQVQAQRKTARLPLAEVWRTARVPV
LLCIGANTIGIAGVYFTNTFMIAYTTQYLSLPRTLILDCLFAVAIIQFLAQPLAAWLAEKLGGARFLKLAALLAMASPYP
MFLLVQTRQAPAIVLGIALAVVCMASFYSVIAGFVSGVFETRVRYSAISLSYQVCGAVAGGLTPLVGTVLAQRFQGQWWP
LAVFYTLLAGVSLLCIASIDARQRQQPQPAAQAAQA

Sequences:

>Translated_436_residues
MQVSPSGTRQPVRAATAAFVGTMIEWYDFYIYATAAALVFGPLFFPGGDPFLSTLASFGTFAVGFFARPLGGIVFGHWGD
RIGRKQALMITLMMMGVATIGIGLLPTYATAGAIAPVLLVLLRVVQGIAVGGEWGGAVLMAGEHAPKGRRTFFASFAQLG
SPAGLILSLLAFRAVTSMEHEVFMDWGWRLPFLASAALLLVGVAIRLGVNESPEFAQVQAQRKTARLPLAEVWRTARVPV
LLCIGANTIGIAGVYFTNTFMIAYTTQYLSLPRTLILDCLFAVAIIQFLAQPLAAWLAEKLGGARFLKLAALLAMASPYP
MFLLVQTRQAPAIVLGIALAVVCMASFYSVIAGFVSGVFETRVRYSAISLSYQVCGAVAGGLTPLVGTVLAQRFQGQWWP
LAVFYTLLAGVSLLCIASIDARQRQQPQPAAQAAQA
>Mature_436_residues
MQVSPSGTRQPVRAATAAFVGTMIEWYDFYIYATAAALVFGPLFFPGGDPFLSTLASFGTFAVGFFARPLGGIVFGHWGD
RIGRKQALMITLMMMGVATIGIGLLPTYATAGAIAPVLLVLLRVVQGIAVGGEWGGAVLMAGEHAPKGRRTFFASFAQLG
SPAGLILSLLAFRAVTSMEHEVFMDWGWRLPFLASAALLLVGVAIRLGVNESPEFAQVQAQRKTARLPLAEVWRTARVPV
LLCIGANTIGIAGVYFTNTFMIAYTTQYLSLPRTLILDCLFAVAIIQFLAQPLAAWLAEKLGGARFLKLAALLAMASPYP
MFLLVQTRQAPAIVLGIALAVVCMASFYSVIAGFVSGVFETRVRYSAISLSYQVCGAVAGGLTPLVGTVLAQRFQGQWWP
LAVFYTLLAGVSLLCIASIDARQRQQPQPAAQAAQA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=39.4736842105263, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1790550, Length=440, Percent_Identity=31.5909090909091, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789941, Length=432, Percent_Identity=35.4166666666667, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1788942, Length=382, Percent_Identity=31.413612565445, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87082231, Length=227, Percent_Identity=23.3480176211454, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46497; Mature: 46497

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSPSGTRQPVRAATAAFVGTMIEWYDFYIYATAAALVFGPLFFPGGDPFLSTLASFGT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFFARPLGGIVFGHWGDRIGRKQALMITLMMMGVATIGIGLLPTYATAGAIAPVLLV
HHHHHHHHHHCCEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRVVQGIAVGGEWGGAVLMAGEHAPKGRRTFFASFAQLGSPAGLILSLLAFRAVTSMEH
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EVFMDWGWRLPFLASAALLLVGVAIRLGVNESPEFAQVQAQRKTARLPLAEVWRTARVPV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCE
LLCIGANTIGIAGVYFTNTFMIAYTTQYLSLPRTLILDCLFAVAIIQFLAQPLAAWLAEK
EEEECCCCHHHEEHHHHHEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGARFLKLAALLAMASPYPMFLLVQTRQAPAIVLGIALAVVCMASFYSVIAGFVSGVFE
HCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRVRYSAISLSYQVCGAVAGGLTPLVGTVLAQRFQGQWWPLAVFYTLLAGVSLLCIASID
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
ARQRQQPQPAAQAAQA
HHHCCCCCCHHHHCCC
>Mature Secondary Structure
MQVSPSGTRQPVRAATAAFVGTMIEWYDFYIYATAAALVFGPLFFPGGDPFLSTLASFGT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FAVGFFARPLGGIVFGHWGDRIGRKQALMITLMMMGVATIGIGLLPTYATAGAIAPVLLV
HHHHHHHHHHCCEEECCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLRVVQGIAVGGEWGGAVLMAGEHAPKGRRTFFASFAQLGSPAGLILSLLAFRAVTSMEH
HHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EVFMDWGWRLPFLASAALLLVGVAIRLGVNESPEFAQVQAQRKTARLPLAEVWRTARVPV
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCE
LLCIGANTIGIAGVYFTNTFMIAYTTQYLSLPRTLILDCLFAVAIIQFLAQPLAAWLAEK
EEEECCCCHHHEEHHHHHEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGGARFLKLAALLAMASPYPMFLLVQTRQAPAIVLGIALAVVCMASFYSVIAGFVSGVFE
HCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRVRYSAISLSYQVCGAVAGGLTPLVGTVLAQRFQGQWWPLAVFYTLLAGVSLLCIASID
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
ARQRQQPQPAAQAAQA
HHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]