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The map label for this gene is cspA [H]

Identifier: 116695961

GI number: 116695961

Start: 2278445

End: 2278648

Strand: Direct

Name: cspA [H]

Synonym: H16_B2025

Alternate gene names: 116695961

Gene position: 2278445-2278648 (Clockwise)

Preceding gene: 116695960

Following gene: 116695962

Centisome position: 78.23

GC content: 53.92

Gene sequence:

>204_bases
ATGGCGACTGGCATCGTGAAGTGGTTTAACAGTGAAAAGGGCTATGGGTTCATCACGCCGGATGATGGTGGCAAGGACTT
GTTTGCCCACCACAGCGAAATCCAAGGCACCGGGTTCAAATCGCTGGAGGAAGGTGCGAGAGTGGAATTCGAAGTCACGC
AAGGTCAGAAGGGCCCACAAGCGTCAAAGATTCGCCCGCTATAG

Upstream 100 bases:

>100_bases
CGGCGCGCCGTAACGCATCCAACCATATTTTGGCGACGGGGCGATTCTGGGGCGGGACTTCTCAGCACGCAACCTGTGTG
CACCAAATGTCGAGGACGAG

Downstream 100 bases:

>100_bases
TCGACCTGCCCACCTGACGGCCTGAATTGACAAAGCCCTCCACGAATTGCGAAGGGCTTTTCTGTTTTGCGGCTGGTGCC
AGCCGCATCCGGGGGGTCGC

Product: cold shock protein, DNA-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 67; Mature: 66

Protein sequence:

>67_residues
MATGIVKWFNSEKGYGFITPDDGGKDLFAHHSEIQGTGFKSLEEGARVEFEVTQGQKGPQASKIRPL

Sequences:

>Translated_67_residues
MATGIVKWFNSEKGYGFITPDDGGKDLFAHHSEIQGTGFKSLEEGARVEFEVTQGQKGPQASKIRPL
>Mature_66_residues
ATGIVKWFNSEKGYGFITPDDGGKDLFAHHSEIQGTGFKSLEEGARVEFEVTQGQKGPQASKIRPL

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=60, Blast_Score=89, Evalue=4e-20,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=62.5, Blast_Score=89, Evalue=5e-20,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=55.3846153846154, Blast_Score=88, Evalue=9e-20,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=60.9375, Blast_Score=86, Evalue=5e-19,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=56.9230769230769, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=53.7313432835821, Blast_Score=80, Evalue=4e-17,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=51.6129032258064, Blast_Score=78, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=49.1803278688525, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7325; Mature: 7194

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGIVKWFNSEKGYGFITPDDGGKDLFAHHSEIQGTGFKSLEEGARVEFEVTQGQKGPQ
CCCCEEEECCCCCCCEEECCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
ASKIRPL
CCCCCCC
>Mature Secondary Structure 
ATGIVKWFNSEKGYGFITPDDGGKDLFAHHSEIQGTGFKSLEEGARVEFEVTQGQKGPQ
CCCEEEECCCCCCCEEECCCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCC
ASKIRPL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12910271 [H]