Click here to switch to the map view.

The map label for this gene is livK3 [H]

Identifier: 116695944

GI number: 116695944

Start: 2265758

End: 2266933

Strand: Direct

Name: livK3 [H]

Synonym: H16_B2008

Alternate gene names: 116695944

Gene position: 2265758-2266933 (Clockwise)

Preceding gene: 116695941

Following gene: 116695945

Centisome position: 77.79

GC content: 62.24

Gene sequence:

>1176_bases
GTGCGCAAGAAACACACTGCAGTAGCCGCCGCCATCGCAGCCGCAGCGACCGGAATGGTCCTGACGGCAGCACCGGCGCA
GGCCAAGGATGTCGTCAAGATTGCCTTTGTCGGTCCGCTGACCGGTGGCGTGTCGTCGATCGGCCTGGGCGGACGCAATT
CCGCGGACCTGGCCGTGCGCTTGCGCAATGCGGACCCGAAGGCCAGGTACACCTATGAACTGGTGGTGCAGGACGACGAG
TGCCGGCCGAACGTCGGCGTGCAGGTGGCGACCAAGATCGCGGCGGACAAGTCCATCGTTGCCGGCGTGACGCACTTCTG
CTCTGCCGTGGCCATGGGCACAGTGGGCGTCTACAGCCGCTTCGGCATGCCCGCCGTGGTGTGGGGCGCCGTGCTGCCCG
AGGTCACCTATGGCAACAAGTTCAAGGAGATCCATCGCGTCAACGGCACGATGATCAACCAGAGCGAAGTGGCGGCCAAA
TTCATGACCGGGCTGGGCTACAAGAAGTGGGCGATCATCCATGACACCACCGACTACGGCAAGGGCCACAACAAGTATTT
CAGCGAGTTCCTGAAGAAGGACGGCGGCACCATCGTCGGCACGTTCGGCGTGACAGCCGATCAGCAAGATTTCACCACGG
AGCTGACCAAGATCCGCGAACTGAAGCCCGACGTGGTGTATTTTGGCGGCCTCACGCCGCTGGGCGTGCGCATCCGCACG
CAGATGGACAAGCTCGGCATCAAGGCTCAGTTCGAGGGCACGTCGGGCATCAAGTCCGATGCCTATATCGAAGGCGTGGG
CAAATCGCTGTCGGAGGGTTCGCTGGCGTTCATCGAAGGCGCGCCGGTAGAGAAGCTGGCGGGCGGCCAGTTCTTCATGG
AACGCTACACGCAGCAGAAATACGGTGAACCGCCGGAAGCGTACGGCCCGTTTGCGTTTGCCGCGGCCAACCTGATCATC
GACGCCGTGGAAAAGGTGGGCCCGGACCGCAAGAAGGTGCGTGATGTGCTGAACGGCACCAGGGACGTGAACACCATCAT
CGGCAAGGTCACCTTCGACGACCACGGCCAGAACGTGGTGCCGCTGATCACCAAGTATGTGGTGGAAGACGGCAAGTGGG
TGATCTGGGAAGACAGCACCTACGGCAAGGGCAAGAAGAAGCTGACCGGCCTGTAA

Upstream 100 bases:

>100_bases
AAGAGCTTTCCCGGGCGTCCACACGCGCCTTGCATCGGTTCGGGTCGGTGACGCCGCGCAGCGGTCCGTCCAATTGTCCA
TGCAACCCAGGGGGAAGAGA

Downstream 100 bases:

>100_bases
GCATGGCGGCCGGGTTCGGGGCAATCCTGCCCCCGCCCGGCCCCGCGCCCGCCTGGGCATCCGGAATTCGGGGGATACCG
ATGAGTGTATTAGGGCAGTA

Product: ABC transporter periplasmic protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRKKHTAVAAAIAAAATGMVLTAAPAQAKDVVKIAFVGPLTGGVSSIGLGGRNSADLAVRLRNADPKARYTYELVVQDDE
CRPNVGVQVATKIAADKSIVAGVTHFCSAVAMGTVGVYSRFGMPAVVWGAVLPEVTYGNKFKEIHRVNGTMINQSEVAAK
FMTGLGYKKWAIIHDTTDYGKGHNKYFSEFLKKDGGTIVGTFGVTADQQDFTTELTKIRELKPDVVYFGGLTPLGVRIRT
QMDKLGIKAQFEGTSGIKSDAYIEGVGKSLSEGSLAFIEGAPVEKLAGGQFFMERYTQQKYGEPPEAYGPFAFAAANLII
DAVEKVGPDRKKVRDVLNGTRDVNTIIGKVTFDDHGQNVVPLITKYVVEDGKWVIWEDSTYGKGKKKLTGL

Sequences:

>Translated_391_residues
MRKKHTAVAAAIAAAATGMVLTAAPAQAKDVVKIAFVGPLTGGVSSIGLGGRNSADLAVRLRNADPKARYTYELVVQDDE
CRPNVGVQVATKIAADKSIVAGVTHFCSAVAMGTVGVYSRFGMPAVVWGAVLPEVTYGNKFKEIHRVNGTMINQSEVAAK
FMTGLGYKKWAIIHDTTDYGKGHNKYFSEFLKKDGGTIVGTFGVTADQQDFTTELTKIRELKPDVVYFGGLTPLGVRIRT
QMDKLGIKAQFEGTSGIKSDAYIEGVGKSLSEGSLAFIEGAPVEKLAGGQFFMERYTQQKYGEPPEAYGPFAFAAANLII
DAVEKVGPDRKKVRDVLNGTRDVNTIIGKVTFDDHGQNVVPLITKYVVEDGKWVIWEDSTYGKGKKKLTGL
>Mature_391_residues
MRKKHTAVAAAIAAAATGMVLTAAPAQAKDVVKIAFVGPLTGGVSSIGLGGRNSADLAVRLRNADPKARYTYELVVQDDE
CRPNVGVQVATKIAADKSIVAGVTHFCSAVAMGTVGVYSRFGMPAVVWGAVLPEVTYGNKFKEIHRVNGTMINQSEVAAK
FMTGLGYKKWAIIHDTTDYGKGHNKYFSEFLKKDGGTIVGTFGVTADQQDFTTELTKIRELKPDVVYFGGLTPLGVRIRT
QMDKLGIKAQFEGTSGIKSDAYIEGVGKSLSEGSLAFIEGAPVEKLAGGQFFMERYTQQKYGEPPEAYGPFAFAAANLII
DAVEKVGPDRKKVRDVLNGTRDVNTIIGKVTFDDHGQNVVPLITKYVVEDGKWVIWEDSTYGKGKKKLTGL

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=360, Percent_Identity=27.7777777777778, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI48994941, Length=270, Percent_Identity=30, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42072; Mature: 42072

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKHTAVAAAIAAAATGMVLTAAPAQAKDVVKIAFVGPLTGGVSSIGLGGRNSADLAVR
CCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEEE
LRNADPKARYTYELVVQDDECRPNVGVQVATKIAADKSIVAGVTHFCSAVAMGTVGVYSR
EECCCCCCEEEEEEEEECCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FGMPAVVWGAVLPEVTYGNKFKEIHRVNGTMINQSEVAAKFMTGLGYKKWAIIHDTTDYG
CCCHHHHHHHHHCCCCCCCHHHHHHHCCCCEECHHHHHHHHHHCCCCCEEEEEECCCCCC
KGHNKYFSEFLKKDGGTIVGTFGVTADQQDFTTELTKIRELKPDVVYFGGLTPLGVRIRT
CCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEE
QMDKLGIKAQFEGTSGIKSDAYIEGVGKSLSEGSLAFIEGAPVEKLAGGQFFMERYTQQK
HHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCEEEECCCCHHHHCCHHHHHHHHHHHH
YGEPPEAYGPFAFAAANLIIDAVEKVGPDRKKVRDVLNGTRDVNTIIGKVTFDDHGQNVV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHEEEEEECCCCCCHH
PLITKYVVEDGKWVIWEDSTYGKGKKKLTGL
HHHHHHHHCCCCEEEECCCCCCCCCHHCCCC
>Mature Secondary Structure
MRKKHTAVAAAIAAAATGMVLTAAPAQAKDVVKIAFVGPLTGGVSSIGLGGRNSADLAVR
CCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCCCHHHCCCCCCCCCEEEEE
LRNADPKARYTYELVVQDDECRPNVGVQVATKIAADKSIVAGVTHFCSAVAMGTVGVYSR
EECCCCCCEEEEEEEEECCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FGMPAVVWGAVLPEVTYGNKFKEIHRVNGTMINQSEVAAKFMTGLGYKKWAIIHDTTDYG
CCCHHHHHHHHHCCCCCCCHHHHHHHCCCCEECHHHHHHHHHHCCCCCEEEEEECCCCCC
KGHNKYFSEFLKKDGGTIVGTFGVTADQQDFTTELTKIRELKPDVVYFGGLTPLGVRIRT
CCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEE
QMDKLGIKAQFEGTSGIKSDAYIEGVGKSLSEGSLAFIEGAPVEKLAGGQFFMERYTQQK
HHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCEEEECCCCHHHHCCHHHHHHHHHHHH
YGEPPEAYGPFAFAAANLIIDAVEKVGPDRKKVRDVLNGTRDVNTIIGKVTFDDHGQNVV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHEEEEEECCCCCCHH
PLITKYVVEDGKWVIWEDSTYGKGKKKLTGL
HHHHHHHHCCCCEEEECCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]