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The map label for this gene is ynfL [H]

Identifier: 116695917

GI number: 116695917

Start: 2241391

End: 2242326

Strand: Direct

Name: ynfL [H]

Synonym: H16_B1981

Alternate gene names: 116695917

Gene position: 2241391-2242326 (Clockwise)

Preceding gene: 116695915

Following gene: 116695919

Centisome position: 76.96

GC content: 66.67

Gene sequence:

>936_bases
ATGTTCAAGGCGCGCGAAGGCTCGGAAAGACTGGCCAAGGAAGTCACGCTGAGGCAGTTCCGCTACTTCGTCGCGGCCGC
GGAGACCGGGCAATTCTCGATGGCCGCGACCGCCGAGCATGTCTCGCAGTCGGCCATCACCAATGCCGTGCTGGCGCTTG
AGCAGCGCCTGGCGGTACGCCTGTTCGAGCGCCGGCCCCATGGGGTGACGTTGACGGCAGAAGGCCACCTCTTCTTCCAG
CACGCCCGCCAGATCCTGGATTCGGTCGAGGATGCGCTGCGCGAGCCGCGCTTCCAGGTGCACGGGTTGCAGGGCAGCGT
GCGCCTTGCCGCCTCCTATACGGTGCTGGGCTACTTCCTGCCGGGGCTGCTGGCGCGCTTTCGCACCAACTATCCGGACA
TCGAACTGGATCTGCTCGACATGGACCGGCCCGATATCGAGCGGGCCGTGCTGGCGGGGGAGATCGAGCTGGGCATCGCG
CTGCTGTCGAACCTGGAGCGGCCCCAGCGCTTCCAGCGCCATACGCTGATGCGATCCCGGCGCCAGTTGTGGACTTCGTC
CAGCCATCCGCTGCTGGCCGTCGAGCGCCCTTCGCTGCGCGATATTGCGGCCTATCCGTATATCCTCATCACCGTGGACG
AGGCCGAGGAATCCACGCTGCGTTACTGGCGCAGCCACCGGCTCGCACCCAAGGTCGCATTCCGGACCGGCTCGATGGAG
GCCCTGCGCGGGCTGGTGGCGCATGGCTTCGGCGTCACCGTGCTGTCCGACATGGTGTATCGCCCGTGGTCACTGGAGGG
AAAGCAGATCGAGGCCCGCCCGATTGCCAATGCGGTGCCGGACATGGAGGTTGGCATGCTGTGGCAGCCCGGGCGCAAGC
TGGGCAAGCCGGCGGATGCGCTGAGGGAGTTCCTGATTCACGCGTGCGGGAGCTGA

Upstream 100 bases:

>100_bases
GTTCTCTTGTATCCGGAGCAGTTTAGAAATCCGTGCCGTCATCGTAAAATCATGAATTCAAACACCTGATATCTGAAAAA
CGGATAACCCGGAGATTCCC

Downstream 100 bases:

>100_bases
GGCGGGACATTCGATTGCCCGCGCCCTTACAACAACGCTATCTGAAGGACCGGTAGCCTGTCGTCGAGTAGTCCGTGTAG
AAGTCCGCCAGGCAATGCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MFKAREGSERLAKEVTLRQFRYFVAAAETGQFSMAATAEHVSQSAITNAVLALEQRLAVRLFERRPHGVTLTAEGHLFFQ
HARQILDSVEDALREPRFQVHGLQGSVRLAASYTVLGYFLPGLLARFRTNYPDIELDLLDMDRPDIERAVLAGEIELGIA
LLSNLERPQRFQRHTLMRSRRQLWTSSSHPLLAVERPSLRDIAAYPYILITVDEAEESTLRYWRSHRLAPKVAFRTGSME
ALRGLVAHGFGVTVLSDMVYRPWSLEGKQIEARPIANAVPDMEVGMLWQPGRKLGKPADALREFLIHACGS

Sequences:

>Translated_311_residues
MFKAREGSERLAKEVTLRQFRYFVAAAETGQFSMAATAEHVSQSAITNAVLALEQRLAVRLFERRPHGVTLTAEGHLFFQ
HARQILDSVEDALREPRFQVHGLQGSVRLAASYTVLGYFLPGLLARFRTNYPDIELDLLDMDRPDIERAVLAGEIELGIA
LLSNLERPQRFQRHTLMRSRRQLWTSSSHPLLAVERPSLRDIAAYPYILITVDEAEESTLRYWRSHRLAPKVAFRTGSME
ALRGLVAHGFGVTVLSDMVYRPWSLEGKQIEARPIANAVPDMEVGMLWQPGRKLGKPADALREFLIHACGS
>Mature_311_residues
MFKAREGSERLAKEVTLRQFRYFVAAAETGQFSMAATAEHVSQSAITNAVLALEQRLAVRLFERRPHGVTLTAEGHLFFQ
HARQILDSVEDALREPRFQVHGLQGSVRLAASYTVLGYFLPGLLARFRTNYPDIELDLLDMDRPDIERAVLAGEIELGIA
LLSNLERPQRFQRHTLMRSRRQLWTSSSHPLLAVERPSLRDIAAYPYILITVDEAEESTLRYWRSHRLAPKVAFRTGSME
ALRGLVAHGFGVTVLSDMVYRPWSLEGKQIEARPIANAVPDMEVGMLWQPGRKLGKPADALREFLIHACGS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=304, Percent_Identity=27.3026315789474, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI157672245, Length=230, Percent_Identity=30.4347826086957, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=27.1812080536913, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081904, Length=205, Percent_Identity=29.7560975609756, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=303, Percent_Identity=27.3927392739274, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787601, Length=272, Percent_Identity=23.8970588235294, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693105, Length=145, Percent_Identity=31.7241379310345, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790399, Length=281, Percent_Identity=23.8434163701068, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788748, Length=146, Percent_Identity=31.5068493150685, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790208, Length=252, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082132, Length=253, Percent_Identity=28.0632411067194, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787949, Length=266, Percent_Identity=25.187969924812, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786401, Length=265, Percent_Identity=25.6603773584906, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790262, Length=220, Percent_Identity=26.8181818181818, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=24.8, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789204, Length=294, Percent_Identity=25.1700680272109, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788626, Length=289, Percent_Identity=26.2975778546713, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788297, Length=260, Percent_Identity=25.7692307692308, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=131, Percent_Identity=29.0076335877863, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787128, Length=128, Percent_Identity=31.25, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35144; Mature: 35144

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKAREGSERLAKEVTLRQFRYFVAAAETGQFSMAATAEHVSQSAITNAVLALEQRLAVR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFERRPHGVTLTAEGHLFFQHARQILDSVEDALREPRFQVHGLQGSVRLAASYTVLGYFL
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHH
PGLLARFRTNYPDIELDLLDMDRPDIERAVLAGEIELGIALLSNLERPQRFQRHTLMRSR
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RQLWTSSSHPLLAVERPSLRDIAAYPYILITVDEAEESTLRYWRSHRLAPKVAFRTGSME
HHHHCCCCCCEEEECCCCCHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCHHHEECCCHH
ALRGLVAHGFGVTVLSDMVYRPWSLEGKQIEARPIANAVPDMEVGMLWQPGRKLGKPADA
HHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCHHCCCCCCCCCEEECCCHHHCCHHHH
LREFLIHACGS
HHHHHHHHCCC
>Mature Secondary Structure
MFKAREGSERLAKEVTLRQFRYFVAAAETGQFSMAATAEHVSQSAITNAVLALEQRLAVR
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFERRPHGVTLTAEGHLFFQHARQILDSVEDALREPRFQVHGLQGSVRLAASYTVLGYFL
HHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEHHHHHHHHHHH
PGLLARFRTNYPDIELDLLDMDRPDIERAVLAGEIELGIALLSNLERPQRFQRHTLMRSR
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RQLWTSSSHPLLAVERPSLRDIAAYPYILITVDEAEESTLRYWRSHRLAPKVAFRTGSME
HHHHCCCCCCEEEECCCCCHHHHHCCEEEEEECCCHHHHHHHHHHCCCCCHHHEECCCHH
ALRGLVAHGFGVTVLSDMVYRPWSLEGKQIEARPIANAVPDMEVGMLWQPGRKLGKPADA
HHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCCHHCCCCCCCCCEEECCCHHHCCHHHH
LREFLIHACGS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]