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The map label for this gene is mdlB [H]

Identifier: 116695768

GI number: 116695768

Start: 2071163

End: 2072320

Strand: Direct

Name: mdlB [H]

Synonym: H16_B1832

Alternate gene names: 116695768

Gene position: 2071163-2072320 (Clockwise)

Preceding gene: 116695767

Following gene: 116695769

Centisome position: 71.11

GC content: 71.16

Gene sequence:

>1158_bases
ATGCTGCCGGCCATCAAGGACTATCGGGACCTGGCGCGGCGCCGGCTGTCGCGCTTCGCCTTCGACTATCTGGAGGGAGG
CGCGGAGGACGGGCGCACGCTGGCACGCAACCTGGCCGCATACCAGGCACTGCTGTTCCGGCCGCGCGTGCTGCGCGACG
TCACGGAGACCGATCCCGGCATGGAGATCTTCGGCCGCAAGTACCGCCTGCCGTTGCTGGTGGGCCCCACGGGGCTGAAC
GGACTGTACTGGCCGAAGGCCGAAGAGGCGCTGGCACGGGCCGCACACGCTGAAGGACTGCCCTTCGTGATGTCGACTGC
ATCGACCTCGCTGATCGAAGACGTGCGCGCAGCCAGCGACGGCGACCTGTGGCTGCAGCTCTACGTGCAGCGCGACCGCG
CCATCGCCGAGAGCATGATGGCGCGCGCACGCGCCGCCGGCTTCAGCACGCTGATGCTGACCGTCGACACCATGGTGCAC
GGCAAGCGCGACCATGACATCCGCAACGGCTTTCGCATGCCGGTGCCGTGGACGCCGCGGCTGCTGGCCGACCTCGCGGC
ACATCCGCGGTGGTGCCTGCGCATGCTGCGCCAGGGCGGCTCGCCGCAGCTGGTCAACCTGGCCCGCAGCAGCGGGATGG
CCAACGACCTGAAGGCGCAGGCGGCGGGATTGAGCCGGCAGATGGACATGTCGCTGTGCTGGGACGACATCGCCTGGCTG
CGCCGGCACTGGCACGGGCCCGTCATCATCAAGGGTATCCTTACGCCGGCCGACGCGGAAATCGCCGCCCGCCAGGGCCT
GGATGGCATCGTGGTCAGCAACCACGGCGGCCGCCAGCTGGAAGGCGCGCCCAGCGCGGTCGAGATGCTGCCCGCCATCG
TCGCGGCGGCGGGCGGGATGCACGTATTCGTCGATGGCGGCGTGCGCCGCGGCGCCGACATCGCCAAGGCGCTGGCAATG
GGCGCCCGCGGCGTGCTGGTGGGGCGCGCACCGCTGTACGGGCTGGCGGCGCGCGGTCCCCGCGGCGTGGCGGAAGTCCT
GGCCATCCTGCGTGGAGAATTCGAGACCACGCTGCGGCTGCTCGGCGTTCCGCAGGCAGCCAGGCTGGACGCTGCCGCGC
TCTCGGACGACCATGCCGCGCGGCTGGCCGCACTGTAA

Upstream 100 bases:

>100_bases
CCGCTGGTAGCGCCGGCACCCGGGCTGCAGGCACAGCTGCGTGCCGAACTGACGCAGCGGCTGCAAGCCGAGGCCGCCCT
CGCCTGAGGCGCCGACGATC

Downstream 100 bases:

>100_bases
GCCATGAAAACAATGACAAGGACGAGCAGGAGGTTGTGTGGATTCAGGTAATGAAGGCAACGGGCTGCGCACAAGGCGCG
AGCGTTCGTCCCTGTTCGTC

Product: L-mandelate dehydrogenase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLPAIKDYRDLARRRLSRFAFDYLEGGAEDGRTLARNLAAYQALLFRPRVLRDVTETDPGMEIFGRKYRLPLLVGPTGLN
GLYWPKAEEALARAAHAEGLPFVMSTASTSLIEDVRAASDGDLWLQLYVQRDRAIAESMMARARAAGFSTLMLTVDTMVH
GKRDHDIRNGFRMPVPWTPRLLADLAAHPRWCLRMLRQGGSPQLVNLARSSGMANDLKAQAAGLSRQMDMSLCWDDIAWL
RRHWHGPVIIKGILTPADAEIAARQGLDGIVVSNHGGRQLEGAPSAVEMLPAIVAAAGGMHVFVDGGVRRGADIAKALAM
GARGVLVGRAPLYGLAARGPRGVAEVLAILRGEFETTLRLLGVPQAARLDAAALSDDHAARLAAL

Sequences:

>Translated_385_residues
MLPAIKDYRDLARRRLSRFAFDYLEGGAEDGRTLARNLAAYQALLFRPRVLRDVTETDPGMEIFGRKYRLPLLVGPTGLN
GLYWPKAEEALARAAHAEGLPFVMSTASTSLIEDVRAASDGDLWLQLYVQRDRAIAESMMARARAAGFSTLMLTVDTMVH
GKRDHDIRNGFRMPVPWTPRLLADLAAHPRWCLRMLRQGGSPQLVNLARSSGMANDLKAQAAGLSRQMDMSLCWDDIAWL
RRHWHGPVIIKGILTPADAEIAARQGLDGIVVSNHGGRQLEGAPSAVEMLPAIVAAAGGMHVFVDGGVRRGADIAKALAM
GARGVLVGRAPLYGLAARGPRGVAEVLAILRGEFETTLRLLGVPQAARLDAAALSDDHAARLAAL
>Mature_385_residues
MLPAIKDYRDLARRRLSRFAFDYLEGGAEDGRTLARNLAAYQALLFRPRVLRDVTETDPGMEIFGRKYRLPLLVGPTGLN
GLYWPKAEEALARAAHAEGLPFVMSTASTSLIEDVRAASDGDLWLQLYVQRDRAIAESMMARARAAGFSTLMLTVDTMVH
GKRDHDIRNGFRMPVPWTPRLLADLAAHPRWCLRMLRQGGSPQLVNLARSSGMANDLKAQAAGLSRQMDMSLCWDDIAWL
RRHWHGPVIIKGILTPADAEIAARQGLDGIVVSNHGGRQLEGAPSAVEMLPAIVAAAGGMHVFVDGGVRRGADIAKALAM
GARGVLVGRAPLYGLAARGPRGVAEVLAILRGEFETTLRLLGVPQAARLDAAALSDDHAARLAAL

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI54234014, Length=371, Percent_Identity=39.622641509434, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI7705393, Length=371, Percent_Identity=39.622641509434, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI11068137, Length=378, Percent_Identity=38.0952380952381, Blast_Score=248, Evalue=6e-66,
Organism=Escherichia coli, GI1790033, Length=375, Percent_Identity=38.6666666666667, Blast_Score=253, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI193208036, Length=387, Percent_Identity=36.9509043927649, Blast_Score=233, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI32566217, Length=290, Percent_Identity=35.1724137931034, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323587, Length=375, Percent_Identity=32.8, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI78707188, Length=390, Percent_Identity=38.2051282051282, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI281363140, Length=390, Percent_Identity=38.2051282051282, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI78707190, Length=390, Percent_Identity=38.2051282051282, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 41713; Mature: 41713

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAIKDYRDLARRRLSRFAFDYLEGGAEDGRTLARNLAAYQALLFRPRVLRDVTETDPG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCC
MEIFGRKYRLPLLVGPTGLNGLYWPKAEEALARAAHAEGLPFVMSTASTSLIEDVRAASD
HHHHCCCEECEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCC
GDLWLQLYVQRDRAIAESMMARARAAGFSTLMLTVDTMVHGKRDHDIRNGFRMPVPWTPR
CCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHH
LLADLAAHPRWCLRMLRQGGSPQLVNLARSSGMANDLKAQAAGLSRQMDMSLCWDDIAWL
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RRHWHGPVIIKGILTPADAEIAARQGLDGIVVSNHGGRQLEGAPSAVEMLPAIVAAAGGM
HHHCCCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCE
HVFVDGGVRRGADIAKALAMGARGVLVGRAPLYGLAARGPRGVAEVLAILRGEFETTLRL
EEEECCCCCCCHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCHHHHHHHHHCCHHHHHHH
LGVPQAARLDAAALSDDHAARLAAL
HCCCCHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure
MLPAIKDYRDLARRRLSRFAFDYLEGGAEDGRTLARNLAAYQALLFRPRVLRDVTETDPG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHCCCCC
MEIFGRKYRLPLLVGPTGLNGLYWPKAEEALARAAHAEGLPFVMSTASTSLIEDVRAASD
HHHHCCCEECEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCC
GDLWLQLYVQRDRAIAESMMARARAAGFSTLMLTVDTMVHGKRDHDIRNGFRMPVPWTPR
CCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHH
LLADLAAHPRWCLRMLRQGGSPQLVNLARSSGMANDLKAQAAGLSRQMDMSLCWDDIAWL
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
RRHWHGPVIIKGILTPADAEIAARQGLDGIVVSNHGGRQLEGAPSAVEMLPAIVAAAGGM
HHHCCCCEEEEEECCCCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCE
HVFVDGGVRRGADIAKALAMGARGVLVGRAPLYGLAARGPRGVAEVLAILRGEFETTLRL
EEEECCCCCCCHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCHHHHHHHHHCCHHHHHHH
LGVPQAARLDAAALSDDHAARLAAL
HCCCCHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]