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The map label for this gene is potH [H]

Identifier: 116695766

GI number: 116695766

Start: 2068454

End: 2070223

Strand: Direct

Name: potH [H]

Synonym: H16_B1830

Alternate gene names: 116695766

Gene position: 2068454-2070223 (Clockwise)

Preceding gene: 116695765

Following gene: 116695767

Centisome position: 71.02

GC content: 67.12

Gene sequence:

>1770_bases
ATGACCGCCGTCGCCCTGCAACGCCGACCCCTACCCTGGCTGCGGCCCGCCTTGCTGGCCGCGCCGCTGGTGCTCCTGTT
CGTGGTGCTGCTGGTCTACCCGGTCGGCCAGCTGCTGCTGCTGAGCGTCTACAGCGACGGTGCCTTCACGCTGGCCAAGT
ACCGCCAGCTGTTTGCGTCATCGGTCTACGTCGACGTGATGCTGATCACGCTGAAGATCTCGCTCTGGACCACGCTGCTC
TCCGTGGTCGCCGGTTATCCGGTGGCCTACCTGATCTCCTCGCTGTCAAAGGACAGGAAGGCAAAGTGGCTGTTCTGGGT
ACTGCTGTCGTTCTGGACCAGCTTCCTGGTGCGCGCCTTTGCCTGGATCGTGATGCTGGGCCGCAACGGCGTGGTCAACC
AGTTGCTGAGCGCCGCCGGCATCCAGGATGCGCCTTCCAACCTGCTGTACAGCCTCGGCGCCGTGCTGGTGGCGATGGTG
CACGCGCTGATGCCGCTGGCGGTGCTGACCATGCTGTCGGTGATGGAGAACATCGACCGCAACCTCCCGCGCGCGGCGCT
TACGCTTGGGGCACGGCCTGGCACGGCGTTCTGGCGCGTCTACTTCCCGCTCTCGATGCCGGGCGTGGCGGCCGGCGCCA
TCATGGTGTTCGTCACCGCCGTCGGCTTCTTTATCGTGCCCGCGCTGCTGGGCGGACGCAAAGAGATCATGGTCGCCCAG
CTGATCATCGACCAGGTGCAGCAGACCATGAACTGGGGTTTCGCCGGCTCGATCTCGGTGTTGCTGCTGCTGGTCGTGCT
GGCGGTGTTCGCCCTGTATGACAAGGTGCTCGGGCTCTCGTCGATGGCAGGCGGAGCGGAGCAACGCACCCGCTCCGCGG
CCGCGCACGATGGCGTGCTGCATCGGCTCGGTGACAGCGTGCTGGGCGCACTGGGAGCGGTCAGCGACAAGCTGCTGGCA
CTGGTGCCGCGCGGCCGGCGCGAGCCGCAGCCGGGCGACTCGCTGCCGCTGCGCACGATCGTGCTGATGGTGCTGCTGTT
CCTGAGCGTGCCGGCGCTGCTGATGATCCCGCTGTCGTTCGCCGACAAGTCGGGGCTGAACTGGCCGCCGCGCGGCTTCA
CGCTGCAGCACTACCAGGACATGCTCGCCTCGCCGCTGTGGATGCAGGCCGGGCTGCGCTCGCTGCTGGTCGGCCTTGGC
GCCGCGGTGCTGGCGCTGCTGGTCGGCACGCCGGTGGCGTTCCTGCTGGCGCGCTCGCAGATGCGGGGCAAGTCGCTGCT
GCTGGCGTTCGTGCTGTCGCCGATCATCATCCCGCGCATGATCATCGCGGTGGGGATGTTCTACTTCTTCGCCAGGGTCG
GGCTGGTCGGCACCTCGCTCGGTCTGATGATCGGCCACACCGTCATTGCCGTGCCCTACGTCGTCATGACGATGATGGCG
GTGCTGCGCAACTACGACACGCGGCTCGACCTGGCGGCGCAAAGCCTGGGCGCCAGGCCCTGGCAGACGCTGCGCTACGT
GACGCTGCCGATCCTCGCCGCCGGCCTGTTCTCGTCCTTCCTGTTCGCCTTCGCCACCTCGTTCGATGAGCTGACGATCG
CCCTGTTTGCCTCTGGCGGACTGAGCGCCACCCTGCCCAAGCAGTTCTGGGACGAGGTGACGCTGCAGCTTTCACCGGTG
ATTGCCGCGGTGTCTACCTGCCTGTTCGTCTTCGTTACCCTCCTGATCACCTGCGCCGAGCGCTTGCGCCGCAAGAGCGC
CGGGCGCTGA

Upstream 100 bases:

>100_bases
TGTCCTACCCCACCTCGATGGGCGCTTCCCGGCATGCGGTCGGGCAGCAAGTCACGCTTACCTGGCAGCGCAGCGACGCT
GTCGCCGTCCCGGATTCGCT

Downstream 100 bases:

>100_bases
GAACCTTATCCATGAACAACAAGCTTACCGCCGCCGACCTGCGCGGCATCTTCCCCGCCATCCCGACGCCCGTGACGGCC
GACGACAGGATCGACCAGGA

Product: ABC transporter permease

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTAVALQRRPLPWLRPALLAAPLVLLFVVLLVYPVGQLLLLSVYSDGAFTLAKYRQLFASSVYVDVMLITLKISLWTTLL
SVVAGYPVAYLISSLSKDRKAKWLFWVLLSFWTSFLVRAFAWIVMLGRNGVVNQLLSAAGIQDAPSNLLYSLGAVLVAMV
HALMPLAVLTMLSVMENIDRNLPRAALTLGARPGTAFWRVYFPLSMPGVAAGAIMVFVTAVGFFIVPALLGGRKEIMVAQ
LIIDQVQQTMNWGFAGSISVLLLLVVLAVFALYDKVLGLSSMAGGAEQRTRSAAAHDGVLHRLGDSVLGALGAVSDKLLA
LVPRGRREPQPGDSLPLRTIVLMVLLFLSVPALLMIPLSFADKSGLNWPPRGFTLQHYQDMLASPLWMQAGLRSLLVGLG
AAVLALLVGTPVAFLLARSQMRGKSLLLAFVLSPIIIPRMIIAVGMFYFFARVGLVGTSLGLMIGHTVIAVPYVVMTMMA
VLRNYDTRLDLAAQSLGARPWQTLRYVTLPILAAGLFSSFLFAFATSFDELTIALFASGGLSATLPKQFWDEVTLQLSPV
IAAVSTCLFVFVTLLITCAERLRRKSAGR

Sequences:

>Translated_589_residues
MTAVALQRRPLPWLRPALLAAPLVLLFVVLLVYPVGQLLLLSVYSDGAFTLAKYRQLFASSVYVDVMLITLKISLWTTLL
SVVAGYPVAYLISSLSKDRKAKWLFWVLLSFWTSFLVRAFAWIVMLGRNGVVNQLLSAAGIQDAPSNLLYSLGAVLVAMV
HALMPLAVLTMLSVMENIDRNLPRAALTLGARPGTAFWRVYFPLSMPGVAAGAIMVFVTAVGFFIVPALLGGRKEIMVAQ
LIIDQVQQTMNWGFAGSISVLLLLVVLAVFALYDKVLGLSSMAGGAEQRTRSAAAHDGVLHRLGDSVLGALGAVSDKLLA
LVPRGRREPQPGDSLPLRTIVLMVLLFLSVPALLMIPLSFADKSGLNWPPRGFTLQHYQDMLASPLWMQAGLRSLLVGLG
AAVLALLVGTPVAFLLARSQMRGKSLLLAFVLSPIIIPRMIIAVGMFYFFARVGLVGTSLGLMIGHTVIAVPYVVMTMMA
VLRNYDTRLDLAAQSLGARPWQTLRYVTLPILAAGLFSSFLFAFATSFDELTIALFASGGLSATLPKQFWDEVTLQLSPV
IAAVSTCLFVFVTLLITCAERLRRKSAGR
>Mature_588_residues
TAVALQRRPLPWLRPALLAAPLVLLFVVLLVYPVGQLLLLSVYSDGAFTLAKYRQLFASSVYVDVMLITLKISLWTTLLS
VVAGYPVAYLISSLSKDRKAKWLFWVLLSFWTSFLVRAFAWIVMLGRNGVVNQLLSAAGIQDAPSNLLYSLGAVLVAMVH
ALMPLAVLTMLSVMENIDRNLPRAALTLGARPGTAFWRVYFPLSMPGVAAGAIMVFVTAVGFFIVPALLGGRKEIMVAQL
IIDQVQQTMNWGFAGSISVLLLLVVLAVFALYDKVLGLSSMAGGAEQRTRSAAAHDGVLHRLGDSVLGALGAVSDKLLAL
VPRGRREPQPGDSLPLRTIVLMVLLFLSVPALLMIPLSFADKSGLNWPPRGFTLQHYQDMLASPLWMQAGLRSLLVGLGA
AVLALLVGTPVAFLLARSQMRGKSLLLAFVLSPIIIPRMIIAVGMFYFFARVGLVGTSLGLMIGHTVIAVPYVVMTMMAV
LRNYDTRLDLAAQSLGARPWQTLRYVTLPILAAGLFSSFLFAFATSFDELTIALFASGGLSATLPKQFWDEVTLQLSPVI
AAVSTCLFVFVTLLITCAERLRRKSAGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=33.0508474576271, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787369, Length=219, Percent_Identity=32.8767123287671, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787368, Length=230, Percent_Identity=26.0869565217391, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787713, Length=242, Percent_Identity=29.3388429752066, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1787714, Length=203, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787081, Length=241, Percent_Identity=28.6307053941909, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 63809; Mature: 63677

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAVALQRRPLPWLRPALLAAPLVLLFVVLLVYPVGQLLLLSVYSDGAFTLAKYRQLFAS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH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CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]