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The map label for this gene is citA [H]

Identifier: 116695447

GI number: 116695447

Start: 1691753

End: 1693078

Strand: Direct

Name: citA [H]

Synonym: H16_B1506

Alternate gene names: 116695447

Gene position: 1691753-1693078 (Clockwise)

Preceding gene: 116695446

Following gene: 116695448

Centisome position: 58.09

GC content: 66.97

Gene sequence:

>1326_bases
ATGCAAAACAGCGCTGCGCCAACGAGCGCGCCCTACACACCGGCTGCGCCAGCGGCGCCCGCCATCCAGCCCAGCCAGCG
CCGCCGCGCCATCATCGCGACGGTGATCGGCAATGGCCTGGAATGGTTCGATTTCACCGTCTACAGCTTCTTCGCGGTCA
TCATCGCGAAGCTGTTCTTTCCCACCGGCGACGACCTCAGCTCGCTGCTGCTGGCCGTGGCCACCTTCGGCGTGGGCTTC
TTCATGCGCCCGGTGGGCGGCATCGTGCTGGGCATCTACGCCGACCGCGTGGGCCGCAAGGCGGCACTGTCGCTGACCAT
CCTGCTGATGGCGCTCGGCACCACGCTGATCGGCATTGCGCCCACATACGACCAGATCGGCCTCTTCGCCCCGCTGCTGA
TCGTGGTGGCGCGCCTGATGCAGGGCTTCTCCGCCGGCGGCGAGATGGGCGGCGCCACCGCCTTCCTGACCGAGTACGCC
CCGGCGCGCCAGCGTGCCTACTACTCCAGCTGGATCCAGGCCAGCATCGGCGTGGCCGTGCTGCTGGGCGCGGCGGTGGG
CACCTTCGTCACCAGTTCGCTGAGCACCGAGGCACTCAACAGCTGGGGCTGGCGCCTGCCGTTCCTGCTGGGCATCGTGA
TCGGCCCGGTGGGCTACTACATCCGCCACCACCTCGATGAAACCCCGACCTTCCGCGACAACGCCGAGCGCGCCGATTCA
CCGCTCAAGGAAATCGTGCAGGCCTACCCGCGCGAGACCCTGGCCAGCTTCTCGATGGTGATCCTGTGGACGGTGTGCAC
CTATGTGCTGCTGTTCTACATGCCGACCTACGCGGTCAAGGTGCTGAAGGTGCCGCAGGCCGATGGCTTTATCGCCGGCA
TGGCGGGCGGCAGTGCCATCATGGTGTTCGCGCCGCTGGTGGGCCTGCTGGCCGACCGCATCGGCCGGCGCGTGCTGCTG
AGCGGCTCGGCGCTGCTGATCCTGGTGCTGGCGTGGCCGATGTTCGCGTATATCAACCATGCGCCCGGCCTGGCTTCGCT
GCTGGTATTCCAGCTGGTGTTCGGCGTGCTGATCGCCACCTATACCGGCCCGATCCTGGCGGCGTTCTCCGAACTGTTCC
CGGCGCGCGTGCTGTCGACCGGCCTGTCGGTGGCCTACAACTTCGCGGTGACGATCTTCGGTGGCTTTGCCTCGTTCATC
ATCACCTGGCTGATCGCCACCACCGGCAGCAGCATGGCCCCGGCCATCTACGTGATGATCGCCGCCGCCATCAGCCTGGT
CGGCACCCGCTTCGTGCGCGAACCTTCCTACCTGCAACAACGCTGA

Upstream 100 bases:

>100_bases
CGCCCCGCCTTCTCCTAACATCAGCTGCACCATCTGCCCGGCCGGCCTGAGACGACACCGGCCCTTGCCCCCGGCAGCTG
TCACAGAGGAGGAAACACCC

Downstream 100 bases:

>100_bases
CATGTCCCAGAGTCCCCAGATCCTTTTCCACGGCGGCGATGTGCTCGAACCCGCCACGGGCGAGCTCCTGCGCGGCCACG
ATGTCCTGGTTGAAGGCGAG

Product: major facilitator superfamily transporter MHS family protein

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQNSAAPTSAPYTPAAPAAPAIQPSQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGDDLSSLLLAVATFGVGF
FMRPVGGIVLGIYADRVGRKAALSLTILLMALGTTLIGIAPTYDQIGLFAPLLIVVARLMQGFSAGGEMGGATAFLTEYA
PARQRAYYSSWIQASIGVAVLLGAAVGTFVTSSLSTEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRDNAERADS
PLKEIVQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLKVPQADGFIAGMAGGSAIMVFAPLVGLLADRIGRRVLL
SGSALLILVLAWPMFAYINHAPGLASLLVFQLVFGVLIATYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFGGFASFI
ITWLIATTGSSMAPAIYVMIAAAISLVGTRFVREPSYLQQR

Sequences:

>Translated_441_residues
MQNSAAPTSAPYTPAAPAAPAIQPSQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGDDLSSLLLAVATFGVGF
FMRPVGGIVLGIYADRVGRKAALSLTILLMALGTTLIGIAPTYDQIGLFAPLLIVVARLMQGFSAGGEMGGATAFLTEYA
PARQRAYYSSWIQASIGVAVLLGAAVGTFVTSSLSTEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRDNAERADS
PLKEIVQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLKVPQADGFIAGMAGGSAIMVFAPLVGLLADRIGRRVLL
SGSALLILVLAWPMFAYINHAPGLASLLVFQLVFGVLIATYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFGGFASFI
ITWLIATTGSSMAPAIYVMIAAAISLVGTRFVREPSYLQQR
>Mature_441_residues
MQNSAAPTSAPYTPAAPAAPAIQPSQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGDDLSSLLLAVATFGVGF
FMRPVGGIVLGIYADRVGRKAALSLTILLMALGTTLIGIAPTYDQIGLFAPLLIVVARLMQGFSAGGEMGGATAFLTEYA
PARQRAYYSSWIQASIGVAVLLGAAVGTFVTSSLSTEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRDNAERADS
PLKEIVQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLKVPQADGFIAGMAGGSAIMVFAPLVGLLADRIGRRVLL
SGSALLILVLAWPMFAYINHAPGLASLLVFQLVFGVLIATYTGPILAAFSELFPARVLSTGLSVAYNFAVTIFGGFASFI
ITWLIATTGSSMAPAIYVMIAAAISLVGTRFVREPSYLQQR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=34.1176470588235, Blast_Score=271, Evalue=9e-74,
Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=31.1163895486936, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789941, Length=433, Percent_Identity=35.1039260969977, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788942, Length=388, Percent_Identity=34.7938144329897, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI87082231, Length=223, Percent_Identity=26.457399103139, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=23.0569948186528, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47148; Mature: 47148

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MQNSAAPTSAPYTPAAPAAPAIQPSQRRRAIIATVIGNGLEWFDFTVYSFFAVIIAKLFF
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
PTGDDLSSLLLAVATFGVGFFMRPVGGIVLGIYADRVGRKAALSLTILLMALGTTLIGIA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
PTYDQIGLFAPLLIVVARLMQGFSAGGEMGGATAFLTEYAPARQRAYYSSWIQASIGVAV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
LLGAAVGTFVTSSLSTEALNSWGWRLPFLLGIVIGPVGYYIRHHLDETPTFRDNAERADS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
PLKEIVQAYPRETLASFSMVILWTVCTYVLLFYMPTYAVKVLKVPQADGFIAGMAGGSAI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHH
MVFAPLVGLLADRIGRRVLLSGSALLILVLAWPMFAYINHAPGLASLLVFQLVFGVLIAT
HHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YTGPILAAFSELFPARVLSTGLSVAYNFAVTIFGGFASFIITWLIATTGSSMAPAIYVMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
AAAISLVGTRFVREPSYLQQR
HHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]