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The map label for this gene is yafC [C]

Identifier: 116695242

GI number: 116695242

Start: 1453283

End: 1454215

Strand: Direct

Name: yafC [C]

Synonym: H16_B1298

Alternate gene names: 116695242

Gene position: 1453283-1454215 (Clockwise)

Preceding gene: 116695235

Following gene: 116695243

Centisome position: 49.9

GC content: 69.67

Gene sequence:

>933_bases
ATGTTCAAGCTAGAAGGTGTCGCCAGCTTTGTCGCCGTGGTCGAGGCAGGCTCCATCAGCGAGGCCGCGAGGCGGCTCAA
CTTGTCCAAGTCCGTTGTCAGCGAACGCCTGGCCGAGCTTGAAAAGGCGATGGGTGCCAACCTGCTGCGCCGGACCACGC
GGCGGCTGTCGCTGACCGAGGATGGCGCCGCCTTCCTGGAGCGGGCCACGCGCATCCTGCATGAAGTGGAGGCGGCGGCA
ACCGATGTTGCCGAGCGGCGCGGCACCCTGGCCGGGTCGTTGCGGATTTCCGCGCCGGTCACATTTGGCCGGATGCACCT
GGGGCCTGCCATCTATCCGTTCCTGGCCCGGCATCCGGAACTGGAGCTGAGCCTGGACCTGGACGACCGCCGCGTGGACG
CGGCCACCGACAGTTATGACGCCGTGGTGCGGCACGGCCCGCTGGAGAGCTCGCGCCTGGTGGCATGGCAGCTTGCCCCC
AGCCACCGCCTCCTGGTCGCATCCCCGGATTACCTGGCGCAGATGGGCGCGCCGGCAACGCTGGCGGAGCTGCAGCAGCA
CCGTGGCATCTTCTACGCCAACCGCGGCGCGGCTGACTGGCGCCTGCACGGCCCCGACGGCACCGAAATCGCCCGTGCCG
CCGTTGCGCTGCGCGTCAACAACGGCGACATGATGCGAGATGCCGCCATCGCGGGAGTCGGCATCGCGTTGTTGCCGACC
TTTATTGCCGGCGATGCCATCCGGCAGGGGGCGCTGGCCGTGATCGAGGTCGGCCTGCGGGCCGAGCCGGAATCCATTTA
TATCGCCCACCCGGAAGGCCGGCGGGTGTCGGCGCGGCTGCGCGCGTTTGCGGAGTGCGTGCGCACGGCCATCGGCAGTC
CGCCGTACTGGGACGCATGGGCGCAGGCACGCGGGGAGGGCGCGGGAACCTGA

Upstream 100 bases:

>100_bases
AGCAGGAACCGTCCTGCTGCCTCGTGAAACCAGTATGGGCAACGCGCGTGGTTCGAACTAGACTGAAGATCGCGGAACAA
CTTTCCGGATATGCCGAACA

Downstream 100 bases:

>100_bases
TGCCGGCTGCTGGCCGGGTTCCACGATCGCCATGTGGCGGCCCTGCAAGCCTGGAGGTTTGCCGCCCGCGCCGCGCGGCC
TGTATTCTAGTGCTCTGCCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MFKLEGVASFVAVVEAGSISEAARRLNLSKSVVSERLAELEKAMGANLLRRTTRRLSLTEDGAAFLERATRILHEVEAAA
TDVAERRGTLAGSLRISAPVTFGRMHLGPAIYPFLARHPELELSLDLDDRRVDAATDSYDAVVRHGPLESSRLVAWQLAP
SHRLLVASPDYLAQMGAPATLAELQQHRGIFYANRGAADWRLHGPDGTEIARAAVALRVNNGDMMRDAAIAGVGIALLPT
FIAGDAIRQGALAVIEVGLRAEPESIYIAHPEGRRVSARLRAFAECVRTAIGSPPYWDAWAQARGEGAGT

Sequences:

>Translated_310_residues
MFKLEGVASFVAVVEAGSISEAARRLNLSKSVVSERLAELEKAMGANLLRRTTRRLSLTEDGAAFLERATRILHEVEAAA
TDVAERRGTLAGSLRISAPVTFGRMHLGPAIYPFLARHPELELSLDLDDRRVDAATDSYDAVVRHGPLESSRLVAWQLAP
SHRLLVASPDYLAQMGAPATLAELQQHRGIFYANRGAADWRLHGPDGTEIARAAVALRVNNGDMMRDAAIAGVGIALLPT
FIAGDAIRQGALAVIEVGLRAEPESIYIAHPEGRRVSARLRAFAECVRTAIGSPPYWDAWAQARGEGAGT
>Mature_310_residues
MFKLEGVASFVAVVEAGSISEAARRLNLSKSVVSERLAELEKAMGANLLRRTTRRLSLTEDGAAFLERATRILHEVEAAA
TDVAERRGTLAGSLRISAPVTFGRMHLGPAIYPFLARHPELELSLDLDDRRVDAATDSYDAVVRHGPLESSRLVAWQLAP
SHRLLVASPDYLAQMGAPATLAELQQHRGIFYANRGAADWRLHGPDGTEIARAAVALRVNNGDMMRDAAIAGVGIALLPT
FIAGDAIRQGALAVIEVGLRAEPESIYIAHPEGRRVSARLRAFAECVRTAIGSPPYWDAWAQARGEGAGT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=35.7142857142857, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=31.25, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=30.3135888501742, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=27.9461279461279, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789639, Length=283, Percent_Identity=28.6219081272085, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1787589, Length=292, Percent_Identity=28.4246575342466, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=27.0833333333333, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787601, Length=123, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI157672245, Length=155, Percent_Identity=30.3225806451613, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=23.6111111111111, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=24.5733788395904, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082132, Length=285, Percent_Identity=24.2105263157895, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787806, Length=246, Percent_Identity=29.6747967479675, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789204, Length=123, Percent_Identity=32.520325203252, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33371; Mature: 33371

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLEGVASFVAVVEAGSISEAARRLNLSKSVVSERLAELEKAMGANLLRRTTRRLSLTE
CCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGAAFLERATRILHEVEAAATDVAERRGTLAGSLRISAPVTFGRMHLGPAIYPFLARHPE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEECHHHHCHHHHHHHHCCCC
LELSLDLDDRRVDAATDSYDAVVRHGPLESSRLVAWQLAPSHRLLVASPDYLAQMGAPAT
EEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCHH
LAELQQHRGIFYANRGAADWRLHGPDGTEIARAAVALRVNNGDMMRDAAIAGVGIALLPT
HHHHHHHCCEEEECCCCCCCEEECCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHH
FIAGDAIRQGALAVIEVGLRAEPESIYIAHPEGRRVSARLRAFAECVRTAIGSPPYWDAW
HHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
AQARGEGAGT
HHHCCCCCCC
>Mature Secondary Structure
MFKLEGVASFVAVVEAGSISEAARRLNLSKSVVSERLAELEKAMGANLLRRTTRRLSLTE
CCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGAAFLERATRILHEVEAAATDVAERRGTLAGSLRISAPVTFGRMHLGPAIYPFLARHPE
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEECHHHHCHHHHHHHHCCCC
LELSLDLDDRRVDAATDSYDAVVRHGPLESSRLVAWQLAPSHRLLVASPDYLAQMGAPAT
EEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCHH
LAELQQHRGIFYANRGAADWRLHGPDGTEIARAAVALRVNNGDMMRDAAIAGVGIALLPT
HHHHHHHCCEEEECCCCCCCEEECCCCHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHH
FIAGDAIRQGALAVIEVGLRAEPESIYIAHPEGRRVSARLRAFAECVRTAIGSPPYWDAW
HHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
AQARGEGAGT
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]