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The map label for this gene is bcr [H]

Identifier: 116695176

GI number: 116695176

Start: 1391882

End: 1393087

Strand: Direct

Name: bcr [H]

Synonym: H16_B1232

Alternate gene names: 116695176

Gene position: 1391882-1393087 (Clockwise)

Preceding gene: 116695174

Following gene: 116695179

Centisome position: 47.79

GC content: 69.24

Gene sequence:

>1206_bases
ATGCTCCGTTCCACCAGCGCTGCCTCGGCCGCGCGCTTTCCTGCCTGGCTGCTGATCTGCGGTTCGCTGATGGCGATCGC
GCCGTTGTCGATCGACATGTACCTGCCGAGTTTTCCGTCGCTGGCCGGCGATCTCGGCGTGGACATCGGCCAGGTGCAGC
TGACCCTCGGCACATTCCTGGTGGGGCTGGCGCTGGGGCAGGCGTTCTATGGGCCGTTCAGCGACCGCTTCGGGCGCAAG
CCACCGCTCTATGTCGGCCTGTGCCTCTACGTGGCCGCCGCGGTGGGCTGCGCGCTGGCACGCAGCGTGGAGTCGCTGAT
GCTGTGGCGCTTCCTGCAGGCGCTGGGCGGCTGCGCCGGCATGGTGATGGCACGCGCGGTGATCCGCGACCGGCTTGAGG
CGCATGAGTCGGCGCGCGCCTTCTCGTCGCTGATGCTGGTGATGGGGGTTGCGCCGATTCTGGCACCGATCGTCGGCGGC
GCCATTCTCTCGGTGTCGGGCTGGCGCGCGATCTTCTGGGTGCTGGCGGTGGCAGGGATGCTGATCCTGCTGCTGGTACA
CCTGCGCATGGAGGAATCGCTGGACCGGCGCCATGCGGCGCCGCTGAGGCTCGGCACGGTGGCGAAAAGCTACGCCGAGC
TGCTGCGGGATCGCGAATTCCTCGGCTATTCGCTGTCTGCGGGTTTCGGCTCGGCGGGCATGTTCGCGTATATCTCCGGC
TCGCCGTTCGTGCTGATCGAGCTGCACGGCATTGCGCCGTCGCACTACGGTTTTGTGTTCGGTGCCAATGCGCTGGGCCT
GATCACGATGTCGCAGCTCAACGCCAGGCTGGTGCGCGGCCGCTCGCTCGATCAGGTGCTCGGGGGCGCGCTGCTGGCTG
CCTGTGCGGCGGGCCTGGCGCTGGCTGTTGCCGCGCTTGCAGGCGTGGCGGTGCTGCCGGTGCTGCTGGCGGGCTTTTTC
GTCTTTATCGGCGCGCTCGGCTGCGTATCGCCGAATGCGTCGGCACTGGCGCTCGCCCATCAGGGCCATCGCGCCGGCAC
GGCTTCGGCGCTGATGGGCACGCTGCAGTTTTCACTCGGGACGCTGGGCGGCGCGGCAGTCAGCCTGTGGCGCGACGGCA
CCGCGCTGCCGCTGGGCGTGGTGATGGCAGCCTGCGGCGCGGGCGCGGTGCTGATGCGATACTACGGGCGCTCAGGCCCC
CGCTGA

Upstream 100 bases:

>100_bases
TGCTGTGCAACAATTGCGTCTTTTCACGGGTTGCAGGCGCGGGCTGCGGCGCAAGCAGGCTGGCCGTGCATGACAACGCC
GTTCCCCCCGGATACTTCCC

Downstream 100 bases:

>100_bases
TACCGTCTGCGAGCCGTCGTCAGGCAGCCTGTTGCGCGGCGGCCTGCTGCGCCAAAGCCTGCTGCACCACCACCATCTGC
GCCGGCATTGGTGCGGCATA

Product: major facilitator superfamily transporter DHA1 family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLRSTSAASAARFPAWLLICGSLMAIAPLSIDMYLPSFPSLAGDLGVDIGQVQLTLGTFLVGLALGQAFYGPFSDRFGRK
PPLYVGLCLYVAAAVGCALARSVESLMLWRFLQALGGCAGMVMARAVIRDRLEAHESARAFSSLMLVMGVAPILAPIVGG
AILSVSGWRAIFWVLAVAGMLILLLVHLRMEESLDRRHAAPLRLGTVAKSYAELLRDREFLGYSLSAGFGSAGMFAYISG
SPFVLIELHGIAPSHYGFVFGANALGLITMSQLNARLVRGRSLDQVLGGALLAACAAGLALAVAALAGVAVLPVLLAGFF
VFIGALGCVSPNASALALAHQGHRAGTASALMGTLQFSLGTLGGAAVSLWRDGTALPLGVVMAACGAGAVLMRYYGRSGP
R

Sequences:

>Translated_401_residues
MLRSTSAASAARFPAWLLICGSLMAIAPLSIDMYLPSFPSLAGDLGVDIGQVQLTLGTFLVGLALGQAFYGPFSDRFGRK
PPLYVGLCLYVAAAVGCALARSVESLMLWRFLQALGGCAGMVMARAVIRDRLEAHESARAFSSLMLVMGVAPILAPIVGG
AILSVSGWRAIFWVLAVAGMLILLLVHLRMEESLDRRHAAPLRLGTVAKSYAELLRDREFLGYSLSAGFGSAGMFAYISG
SPFVLIELHGIAPSHYGFVFGANALGLITMSQLNARLVRGRSLDQVLGGALLAACAAGLALAVAALAGVAVLPVLLAGFF
VFIGALGCVSPNASALALAHQGHRAGTASALMGTLQFSLGTLGGAAVSLWRDGTALPLGVVMAACGAGAVLMRYYGRSGP
R
>Mature_401_residues
MLRSTSAASAARFPAWLLICGSLMAIAPLSIDMYLPSFPSLAGDLGVDIGQVQLTLGTFLVGLALGQAFYGPFSDRFGRK
PPLYVGLCLYVAAAVGCALARSVESLMLWRFLQALGGCAGMVMARAVIRDRLEAHESARAFSSLMLVMGVAPILAPIVGG
AILSVSGWRAIFWVLAVAGMLILLLVHLRMEESLDRRHAAPLRLGTVAKSYAELLRDREFLGYSLSAGFGSAGMFAYISG
SPFVLIELHGIAPSHYGFVFGANALGLITMSQLNARLVRGRSLDQVLGGALLAACAAGLALAVAALAGVAVLPVLLAGFF
VFIGALGCVSPNASALALAHQGHRAGTASALMGTLQFSLGTLGGAAVSLWRDGTALPLGVVMAACGAGAVLMRYYGRSGP
R

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=385, Percent_Identity=37.6623376623377, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI48994889, Length=377, Percent_Identity=31.8302387267904, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1790146, Length=349, Percent_Identity=30.945558739255, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1787065, Length=343, Percent_Identity=27.4052478134111, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082312, Length=330, Percent_Identity=29.6969696969697, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790794, Length=260, Percent_Identity=23.0769230769231, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325414, Length=179, Percent_Identity=29.608938547486, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321577, Length=180, Percent_Identity=29.4444444444444, Blast_Score=63, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=161, Percent_Identity=24.8447204968944, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41514; Mature: 41514

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSTSAASAARFPAWLLICGSLMAIAPLSIDMYLPSFPSLAGDLGVDIGQVQLTLGTFL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCHHHHHHHHHHHH
VGLALGQAFYGPFSDRFGRKPPLYVGLCLYVAAAVGCALARSVESLMLWRFLQALGGCAG
HHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
MVMARAVIRDRLEAHESARAFSSLMLVMGVAPILAPIVGGAILSVSGWRAIFWVLAVAGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
LILLLVHLRMEESLDRRHAAPLRLGTVAKSYAELLRDREFLGYSLSAGFGSAGMFAYISG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCEEEEECC
SPFVLIELHGIAPSHYGFVFGANALGLITMSQLNARLVRGRSLDQVLGGALLAACAAGLA
CCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAVAALAGVAVLPVLLAGFFVFIGALGCVSPNASALALAHQGHRAGTASALMGTLQFSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHHH
TLGGAAVSLWRDGTALPLGVVMAACGAGAVLMRYYGRSGPR
HCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MLRSTSAASAARFPAWLLICGSLMAIAPLSIDMYLPSFPSLAGDLGVDIGQVQLTLGTFL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCHHHHHHHHHHHH
VGLALGQAFYGPFSDRFGRKPPLYVGLCLYVAAAVGCALARSVESLMLWRFLQALGGCAG
HHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
MVMARAVIRDRLEAHESARAFSSLMLVMGVAPILAPIVGGAILSVSGWRAIFWVLAVAGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
LILLLVHLRMEESLDRRHAAPLRLGTVAKSYAELLRDREFLGYSLSAGFGSAGMFAYISG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCEEEEECC
SPFVLIELHGIAPSHYGFVFGANALGLITMSQLNARLVRGRSLDQVLGGALLAACAAGLA
CCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LAVAALAGVAVLPVLLAGFFVFIGALGCVSPNASALALAHQGHRAGTASALMGTLQFSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHHH
TLGGAAVSLWRDGTALPLGVVMAACGAGAVLMRYYGRSGPR
HCCHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]