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The map label for this gene is cpg2 [H]

Identifier: 116695050

GI number: 116695050

Start: 1251430

End: 1252689

Strand: Direct

Name: cpg2 [H]

Synonym: H16_B1105

Alternate gene names: 116695050

Gene position: 1251430-1252689 (Clockwise)

Preceding gene: 116695048

Following gene: 116695051

Centisome position: 42.97

GC content: 70.16

Gene sequence:

>1260_bases
ATGCCGTTTTCCGTTCGCCACGTTGCCGTTGCCGCCGCCCTGACGCTTGCCGGGGCCGGTACCCTGGCCAGTGCCGCCCA
GCCCGATGCCGCGCTGCTGTCCGCCGCGCAGTCGGCCCAGCCCGCGCTGGTGCAGTCGCTCAAGGAGATGGTGTCGATCG
AATCCGGCAGCGCCAACGCGAAGGGGCTGGCGCAGATGGCCGGCTATACCGAGAAGCGCCTGCAGGCGCTGGGCGCGAAG
GTTGAACGCATGCCCGTCACCAAGGGTCCGGGCACCATGGTCAAGGCCACCTTCACCGGTACCGGCAAGCGCCGCATCAT
GCTGATTGCCCACATGGACACGGTGTATCCGGACAACACCCTCGCCACGCAGCCCATCCGCGAGGAAGGCAACCGGCTGT
ACGGGCCGGGCATTGCCGACGACAAGGGCGGCATCGCCGTGATCCTGCACTCGCTGGAGATCCTGAAGAACCAGGGCTGG
CGCGACTATGCGCAGATCACGGTGCTGTTCAACCCGGACGAGGAGGTCGGCTCGGTCGGCTCCGGCGAAGCCATCGCCGC
GCTGGCCGCGCAGCACGACGTGGTGCTGTCGTGCGAGCCGACCGCGGCCAAGGACGTGGTCAAGGCCGAGTCGCTGCTGC
TGGGCGCTTCCGGCACCGCCACCGCCACGATGCAGGTCAAGGGCCGAGCATCCCACGCCGGCGCCGCGCCGGAGCGCGGC
CGCAATGCGCTGCTCGAACTCGCCCACCAGCTGCAGCAGACGCGCGACACCGCCAGCCTGGTGCCGGGCTCGCAGCTGAA
CTGGACCCAGGCGCAGGCCGGCACGGTGCGCAACCAGATCCCGGAAAGCGCTGTCGCCTACGGCGATGTGCGCACCACCG
CGGCCGGCGCCGCCGAGAAGCTGAAGGTGGCGCTGCAGGACAAGGTCAAGTCCGGCCAGCTGGTGCCGGAGACGCAGACC
ACGGTGACGATGGAAGAGGGCCGCCCGCCGTTCGTGGCCGACGCGCGCGGCCGCGCGCTGGCCAAGCGCGCGCAGGAGAT
CTACGCCGAGCTGGACGGCCGCACGCTGGCCCTGGCCGAAGGCACCGGCGGCGCCACTGACGCCAGCTATGCCGCGCGCT
CGGGCAAGCCCGCGGTGGTGGAAAGCTTCGGGCTGGCCGGCTTCGGCTATCACGCCCGTGACGAGTACATCGAACTGGAC
TCGATCGTGCCGCGCCTGTACCTGATGACCCGCATCCTGCAGGATATCGGGCGCAACTGA

Upstream 100 bases:

>100_bases
TCCCCTAAGGTTCTGGCAAGGGCTAGCATCGTGCCGTTGTGCCGCGGCGCCGCCCGTGCGCCGGGGCATGGCACCAACAA
GACCAATCCGAGGAGATTCC

Downstream 100 bases:

>100_bases
GCCGGCGCCGCCAGGCCTGCATTGCCGGCGCCCGGTTGCCCCTCCCGGCAAGCCTGCTACAGTGTCATGGCTGCCCGCAT
GCGGCGGCCATGACAAAAGA

Product: glutamate carboxypeptidase

Products: NA

Alternate protein names: CPDG2; Folate hydrolase G2; Glutamate carboxypeptidase; Pteroylmonoglutamic acid hydrolase G2; Glucarpidase [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPFSVRHVAVAAALTLAGAGTLASAAQPDAALLSAAQSAQPALVQSLKEMVSIESGSANAKGLAQMAGYTEKRLQALGAK
VERMPVTKGPGTMVKATFTGTGKRRIMLIAHMDTVYPDNTLATQPIREEGNRLYGPGIADDKGGIAVILHSLEILKNQGW
RDYAQITVLFNPDEEVGSVGSGEAIAALAAQHDVVLSCEPTAAKDVVKAESLLLGASGTATATMQVKGRASHAGAAPERG
RNALLELAHQLQQTRDTASLVPGSQLNWTQAQAGTVRNQIPESAVAYGDVRTTAAGAAEKLKVALQDKVKSGQLVPETQT
TVTMEEGRPPFVADARGRALAKRAQEIYAELDGRTLALAEGTGGATDASYAARSGKPAVVESFGLAGFGYHARDEYIELD
SIVPRLYLMTRILQDIGRN

Sequences:

>Translated_419_residues
MPFSVRHVAVAAALTLAGAGTLASAAQPDAALLSAAQSAQPALVQSLKEMVSIESGSANAKGLAQMAGYTEKRLQALGAK
VERMPVTKGPGTMVKATFTGTGKRRIMLIAHMDTVYPDNTLATQPIREEGNRLYGPGIADDKGGIAVILHSLEILKNQGW
RDYAQITVLFNPDEEVGSVGSGEAIAALAAQHDVVLSCEPTAAKDVVKAESLLLGASGTATATMQVKGRASHAGAAPERG
RNALLELAHQLQQTRDTASLVPGSQLNWTQAQAGTVRNQIPESAVAYGDVRTTAAGAAEKLKVALQDKVKSGQLVPETQT
TVTMEEGRPPFVADARGRALAKRAQEIYAELDGRTLALAEGTGGATDASYAARSGKPAVVESFGLAGFGYHARDEYIELD
SIVPRLYLMTRILQDIGRN
>Mature_418_residues
PFSVRHVAVAAALTLAGAGTLASAAQPDAALLSAAQSAQPALVQSLKEMVSIESGSANAKGLAQMAGYTEKRLQALGAKV
ERMPVTKGPGTMVKATFTGTGKRRIMLIAHMDTVYPDNTLATQPIREEGNRLYGPGIADDKGGIAVILHSLEILKNQGWR
DYAQITVLFNPDEEVGSVGSGEAIAALAAQHDVVLSCEPTAAKDVVKAESLLLGASGTATATMQVKGRASHAGAAPERGR
NALLELAHQLQQTRDTASLVPGSQLNWTQAQAGTVRNQIPESAVAYGDVRTTAAGAAEKLKVALQDKVKSGQLVPETQTT
VTMEEGRPPFVADARGRALAKRAQEIYAELDGRTLALAEGTGGATDASYAARSGKPAVVESFGLAGFGYHARDEYIELDS
IVPRLYLMTRILQDIGRN

Specific function: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=401, Percent_Identity=25.6857855361596, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR017150
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.17.11 [H]

Molecular weight: Translated: 43891; Mature: 43760

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSVRHVAVAAALTLAGAGTLASAAQPDAALLSAAQSAQPALVQSLKEMVSIESGSANA
CCCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH
KGLAQMAGYTEKRLQALGAKVERMPVTKGPGTMVKATFTGTGKRRIMLIAHMDTVYPDNT
HHHHHHHCCHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCC
LATQPIREEGNRLYGPGIADDKGGIAVILHSLEILKNQGWRDYAQITVLFNPDEEVGSVG
CCCCCHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHCCCC
SGEAIAALAAQHDVVLSCEPTAAKDVVKAESLLLGASGTATATMQVKGRASHAGAAPERG
CCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCHHH
RNALLELAHQLQQTRDTASLVPGSQLNWTQAQAGTVRNQIPESAVAYGDVRTTAAGAAEK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHCCHHHHHHCCCHHHHHCHHHH
LKVALQDKVKSGQLVPETQTTVTMEEGRPPFVADARGRALAKRAQEIYAELDGRTLALAE
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEC
GTGGATDASYAARSGKPAVVESFGLAGFGYHARDEYIELDSIVPRLYLMTRILQDIGRN
CCCCCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PFSVRHVAVAAALTLAGAGTLASAAQPDAALLSAAQSAQPALVQSLKEMVSIESGSANA
CCCHHHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH
KGLAQMAGYTEKRLQALGAKVERMPVTKGPGTMVKATFTGTGKRRIMLIAHMDTVYPDNT
HHHHHHHCCHHHHHHHHCCHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCC
LATQPIREEGNRLYGPGIADDKGGIAVILHSLEILKNQGWRDYAQITVLFNPDEEVGSVG
CCCCCHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHCCCC
SGEAIAALAAQHDVVLSCEPTAAKDVVKAESLLLGASGTATATMQVKGRASHAGAAPERG
CCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHEEECCCCEEEEEEECCCCCCCCCCCHHH
RNALLELAHQLQQTRDTASLVPGSQLNWTQAQAGTVRNQIPESAVAYGDVRTTAAGAAEK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHCCHHHHHHCCCHHHHHCHHHH
LKVALQDKVKSGQLVPETQTTVTMEEGRPPFVADARGRALAKRAQEIYAELDGRTLALAE
HHHHHHHHCCCCCCCCCCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEC
GTGGATDASYAARSGKPAVVESFGLAGFGYHARDEYIELDSIVPRLYLMTRILQDIGRN
CCCCCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6396165; 3838935; 9083113 [H]