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The map label for this gene is pyk3 [H]

Identifier: 116694906

GI number: 116694906

Start: 1082768

End: 1084186

Strand: Direct

Name: pyk3 [H]

Synonym: H16_B0961

Alternate gene names: 116694906

Gene position: 1082768-1084186 (Clockwise)

Preceding gene: 116694905

Following gene: 116694907

Centisome position: 37.18

GC content: 68.5

Gene sequence:

>1419_bases
ATGAGACGCCTTCGCAACGCCAAGATCGTGGCCACGCTGGGCCCCGCGAGTAGCACCCCCGAGCTCATCGACGCGCTGTT
CGAGGCCGGTGCCGATGTCTTCCGGCTCAACTTCAGCCACGGCACCCACGAAGACCACCGGCAACGGTTCGAAGCGATCC
GCGCCGTGGAGCGCAAGCGAGGCCGCCCCATCGGTGTGCTGCTCGACCTGCAGGGTCCGAAGCTGCGCATCGGCACCTTC
GCCGACGGGCCAGTGCAACTGGCCGCGGGAGCCGGCTTCCGGCTGGACCTGGACAGCGCGGCGCCCGGCTCGGCCAGCAG
GGTCTGCCTGCCGCACCCGGAGATCTTCGCCGCGCTGAGCGAAGGCGTCGAGCTGCTGCTGGACGACGGCAAGATCCGGT
TGCGCGTCGAGCAGTGCGGCGCCGGCTTCGCCGAGACCACGGTCGTCAACGGCGGTACGCTCTCGGACCGAAAAGGGGTG
AACGTGCCTGATGCGGTGCTGCCGCTGTCGGCCATGACCGAAAAAGACCGGCGCGACCTCGACTTCGGTCTGGCCCTGGG
GGCGGACTGGATCGCGCTGTCCTTCGTGCAGCGGCCCGAAGACATCCGGGAGATCAAGGCAATTGTCAACGGCCGCGCCG
GCATCGTGGCCAAGCTGGAGAAGCCCGCCGCCATCCAGAGCCTGGATCTGATCGTCGCCGAGGCCGATGCCATCATGGTG
GCGCGCGGCGACCTGGGCGTGGAGATGCCGGCCGAGCAGGTGCCCTCGCTGCAGAAGCAGATCGTCCGCGCATGCCGCAA
GGCCGGCAAGCCGGTCATCGTTGCCACGCAAATGCTCGAATCGATGATTGCCGCCCCGGTGCCCACCCGCGCGGAAGCGT
CAGACGTGGCGACGGCGATCTACGACGGTGCCGACGCGGTCATGCTGTCGGCCGAGTCGGCTTCGGGCCGGTATCCGGTG
GAGGCGGTGCGAATGATGAACAGCATTATCTCCCGCACGGAATCCGACCCGCTGTACCGCGACGCCATCGAGGCATCCCA
CACGCCGCCGCGCGCGGACGCGGCTGATGCCATCGGTTATGCGGTGCGGCATATCGTGGGCTTGCTCAGGGTGCCGGCGA
CGGTCGCCTATACCAGCTCGGGCTATTCGGCGCTGCGTATGGCGCGCGAGCGGCCGCAGGTGCCCATTCTCGGCATGACA
CCGCGCATGGCGACGGCGAGGCGGCTGACGCTCACCTGGGGCGTGCATGCGGTGCTCTGCCACGAGGTGCTGGATGTGCC
GGAGATGACCGATCTGGCCAGCCGCACTGTGCTGGCGGAGGGCTTCGGGGAAGCCGGACAGTCCGTCGTCATCTCCGCGG
GGCTGCCGTTCCTGGTGGCGGGAACCACCAACCTGCTGCGGATTGCGCAGATCCAATGA

Upstream 100 bases:

>100_bases
GACATGACCAGCAAGTTCCCGAGCATCGTCGTGCAGAACACCTGACCAGGGTGAGCGATTTACGCGCGGCGCCCGTCGCG
CGCTGACATGGAGCAATGGC

Downstream 100 bases:

>100_bases
GGGGGTAGGGACACCGCCGAACAGGTACGACCCGGGGCATTGCCGCAGCGTTGCACGGTCCCCGCTGACAGAGGCCACTG
CCACCTCCGTCAGCGGGCCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MRRLRNAKIVATLGPASSTPELIDALFEAGADVFRLNFSHGTHEDHRQRFEAIRAVERKRGRPIGVLLDLQGPKLRIGTF
ADGPVQLAAGAGFRLDLDSAAPGSASRVCLPHPEIFAALSEGVELLLDDGKIRLRVEQCGAGFAETTVVNGGTLSDRKGV
NVPDAVLPLSAMTEKDRRDLDFGLALGADWIALSFVQRPEDIREIKAIVNGRAGIVAKLEKPAAIQSLDLIVAEADAIMV
ARGDLGVEMPAEQVPSLQKQIVRACRKAGKPVIVATQMLESMIAAPVPTRAEASDVATAIYDGADAVMLSAESASGRYPV
EAVRMMNSIISRTESDPLYRDAIEASHTPPRADAADAIGYAVRHIVGLLRVPATVAYTSSGYSALRMARERPQVPILGMT
PRMATARRLTLTWGVHAVLCHEVLDVPEMTDLASRTVLAEGFGEAGQSVVISAGLPFLVAGTTNLLRIAQIQ

Sequences:

>Translated_472_residues
MRRLRNAKIVATLGPASSTPELIDALFEAGADVFRLNFSHGTHEDHRQRFEAIRAVERKRGRPIGVLLDLQGPKLRIGTF
ADGPVQLAAGAGFRLDLDSAAPGSASRVCLPHPEIFAALSEGVELLLDDGKIRLRVEQCGAGFAETTVVNGGTLSDRKGV
NVPDAVLPLSAMTEKDRRDLDFGLALGADWIALSFVQRPEDIREIKAIVNGRAGIVAKLEKPAAIQSLDLIVAEADAIMV
ARGDLGVEMPAEQVPSLQKQIVRACRKAGKPVIVATQMLESMIAAPVPTRAEASDVATAIYDGADAVMLSAESASGRYPV
EAVRMMNSIISRTESDPLYRDAIEASHTPPRADAADAIGYAVRHIVGLLRVPATVAYTSSGYSALRMARERPQVPILGMT
PRMATARRLTLTWGVHAVLCHEVLDVPEMTDLASRTVLAEGFGEAGQSVVISAGLPFLVAGTTNLLRIAQIQ
>Mature_472_residues
MRRLRNAKIVATLGPASSTPELIDALFEAGADVFRLNFSHGTHEDHRQRFEAIRAVERKRGRPIGVLLDLQGPKLRIGTF
ADGPVQLAAGAGFRLDLDSAAPGSASRVCLPHPEIFAALSEGVELLLDDGKIRLRVEQCGAGFAETTVVNGGTLSDRKGV
NVPDAVLPLSAMTEKDRRDLDFGLALGADWIALSFVQRPEDIREIKAIVNGRAGIVAKLEKPAAIQSLDLIVAEADAIMV
ARGDLGVEMPAEQVPSLQKQIVRACRKAGKPVIVATQMLESMIAAPVPTRAEASDVATAIYDGADAVMLSAESASGRYPV
EAVRMMNSIISRTESDPLYRDAIEASHTPPRADAADAIGYAVRHIVGLLRVPATVAYTSSGYSALRMARERPQVPILGMT
PRMATARRLTLTWGVHAVLCHEVLDVPEMTDLASRTVLAEGFGEAGQSVVISAGLPFLVAGTTNLLRIAQIQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=38.1632653061225, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=38.1632653061225, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=38.0081300813008, Blast_Score=308, Evalue=9e-84,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=37.1717171717172, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=37.1717171717172, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=38.5416666666667, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=38.5416666666667, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.0210970464135, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.0210970464135, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=34.2592592592593, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=37.7682403433476, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=37.2141372141372, Blast_Score=278, Evalue=5e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=38.9690721649485, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=38.9690721649485, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=38.9690721649485, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=38.9690721649485, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=36.4389233954451, Blast_Score=283, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6319279, Length=416, Percent_Identity=35.3365384615385, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=33.3333333333333, Blast_Score=234, Evalue=3e-62,
Organism=Drosophila melanogaster, GI28571814, Length=495, Percent_Identity=36.3636363636364, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24648964, Length=495, Percent_Identity=36.3636363636364, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24648966, Length=441, Percent_Identity=35.3741496598639, Blast_Score=239, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24581235, Length=485, Percent_Identity=31.7525773195876, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24646914, Length=264, Percent_Identity=37.5, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50362; Mature: 50362

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLRNAKIVATLGPASSTPELIDALFEAGADVFRLNFSHGTHEDHRQRFEAIRAVERKR
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPIGVLLDLQGPKLRIGTFADGPVQLAAGAGFRLDLDSAAPGSASRVCLPHPEIFAALS
CCCEEEEEECCCCEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCEEECCCHHHHHHHH
EGVELLLDDGKIRLRVEQCGAGFAETTVVNGGTLSDRKGVNVPDAVLPLSAMTEKDRRDL
HCCEEEEECCEEEEEEEHHCCCCCCEEEECCCCCCCCCCCCCCHHHCCHHHHHCCHHCCC
DFGLALGADWIALSFVQRPEDIREIKAIVNGRAGIVAKLEKPAAIQSLDLIVAEADAIMV
CHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHEEEEECCEEEE
ARGDLGVEMPAEQVPSLQKQIVRACRKAGKPVIVATQMLESMIAAPVPTRAEASDVATAI
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHH
YDGADAVMLSAESASGRYPVEAVRMMNSIISRTESDPLYRDAIEASHTPPRADAADAIGY
HCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHH
AVRHIVGLLRVPATVAYTSSGYSALRMARERPQVPILGMTPRMATARRLTLTWGVHAVLC
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCHHHEEEEEEHHHHHHHH
HEVLDVPEMTDLASRTVLAEGFGEAGQSVVISAGLPFLVAGTTNLLRIAQIQ
HHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCEEEEEECC
>Mature Secondary Structure
MRRLRNAKIVATLGPASSTPELIDALFEAGADVFRLNFSHGTHEDHRQRFEAIRAVERKR
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRPIGVLLDLQGPKLRIGTFADGPVQLAAGAGFRLDLDSAAPGSASRVCLPHPEIFAALS
CCCEEEEEECCCCEEEEEECCCCCEEEECCCCEEEECCCCCCCCCCEEECCCHHHHHHHH
EGVELLLDDGKIRLRVEQCGAGFAETTVVNGGTLSDRKGVNVPDAVLPLSAMTEKDRRDL
HCCEEEEECCEEEEEEEHHCCCCCCEEEECCCCCCCCCCCCCCHHHCCHHHHHCCHHCCC
DFGLALGADWIALSFVQRPEDIREIKAIVNGRAGIVAKLEKPAAIQSLDLIVAEADAIMV
CHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHEEEEECCEEEE
ARGDLGVEMPAEQVPSLQKQIVRACRKAGKPVIVATQMLESMIAAPVPTRAEASDVATAI
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHH
YDGADAVMLSAESASGRYPVEAVRMMNSIISRTESDPLYRDAIEASHTPPRADAADAIGY
HCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHH
AVRHIVGLLRVPATVAYTSSGYSALRMARERPQVPILGMTPRMATARRLTLTWGVHAVLC
HHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCHHHEEEEEEHHHHHHHH
HEVLDVPEMTDLASRTVLAEGFGEAGQSVVISAGLPFLVAGTTNLLRIAQIQ
HHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA