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The map label for this gene is gatA [H]

Identifier: 116694829

GI number: 116694829

Start: 1000911

End: 1002341

Strand: Direct

Name: gatA [H]

Synonym: H16_B0883

Alternate gene names: 116694829

Gene position: 1000911-1002341 (Clockwise)

Preceding gene: 116694828

Following gene: 116694830

Centisome position: 34.37

GC content: 71.21

Gene sequence:

>1431_bases
ATGGATGAGCTTTGGAAACTGCCGGCACATGCGCTCACCGACGGCTACGCCACCGGCCGCTTCACGCCGCAACAGGCGCT
GGCGTCCTGCCTGGCGCGCACGGCGCACTGCAATCCGGCGCTGAACGCCGTGATCGCATTCGATACTGAAGGCGCCATGG
CGGCGGCACAGGAGAGCACCGCGCGCTGGCGCCGTGGCGAGCCGCTGGGTGCGCTCGATGGCGTGCCGGTCACCGTCAAG
GACAACCTGCACGTGCGCGGCCTGCCCACGCATTGGGGCAGCCGCGCGCTGGCGGGCTTTGTCCCGGACCGCGACGAACT
ACCGGTGGCGCGCCTGCGCGCGGCCGGGGCGCTGATCTTCGGCAAGACCAATGTGCCGGAGTTCGCCATGCAGGGCTATA
CCGACAACCTCGTCTTCGGCACAACCGGCAATCCCTGGCAGCCGGCCCTGACGCCGGGCGGGTCTTCCGGCGGCGCGGTG
GCGGCGGTGGCATCGGGCTGCTGCCCGCTGGCGCTGGGCACCGACGGCGGCGGCTCGATCCGGCGCCCGGCCTCGCATAC
CGGGCTGGTGGGCTTCAAGCCGTCGCCCGGCCGGCTGCCGCGCGGCGATGGGCTGCCAGAGATCTTCATGCGCTATGAAG
TGGTCGGCCCGCTGGCCCGCTGCGTGGCCGATGCGATGGCCATGGCCGAGGCGCTGGGCCAGCCCGACCCGCGCGATCCC
GATTCCCGGCGCTTTGCCGGCGTGCCGCTGCAGATGCCGCCGCGGCCATGCCGGGTCTTGCACGTGCCCCGGTTCGGCGA
TCATCCGGTGGATGCGCGCATCGCTGCCTTGACCGGGCAGGCAGCGCAAGCGCTGCGTGAACTTGGCCACACGGTGGATA
CCGCGCAGGCGTTCGATCTGGCTGAAGCGACCAATGCCAAATGGTCGCTGTTGTCGCAGGCCGGCCTGGCCTGGCTGGTG
GAGCAGGGCGGCGCGCAACTGCCGTTCTGCCCGCCGCTCGATGCGGGCACGTTCGGTGCTGCCACGCAGGCCAATGCATC
GGCTGGCGCAGGCCTGCGTGCCGTGCAGCTGTTTGATCTGCTGATGGCCGTGGAGCGTCTGCGCAGCGAGCTTTCCTTGC
TGTTCGCACGCTACGACCTGCTGCTGACGCCCGCCGCAGCGGCATTGCCATGGCCGGCCGCACAGTCGCACCCGCCTGTG
ATCGAAGGCCGCGAGGCCGGGCCGCGTGGCCATGCGGTGTTCACCGCATTCGCCAATGCTGCCGGCCTGCCCGCGATCTC
GTTGCCATGCGGCGAGGCAGAGGGCTTGCCGGTTGGCCTCCAGCTGGTGGCGGCGCCGGCCGAGGATGCGTTGCTGCTGG
CAATCGCGCGCCAGTTCGAGCAGGCCTTCCCGAGGGCGCCGGGATTCCCGCTCGACGGGCAGGGCCGATGA

Upstream 100 bases:

>100_bases
GGTCTGCGACAAGTCTTACCCGCAATGCTCGGCCATCTGGAAGCAGACCATCGGGCCGCTGGTCGGGGTCAAGTGAACAC
TGAGGACGGCGGAGACGACG

Downstream 100 bases:

>100_bases
AGACCCTGGTTGCGCCAGGTACGCCGGCGGCGCTCGCGCAGATGCTCTGCGTCGCGCGTACGCGCGGCGAGCGCGTCGAT
CCGGCACTGTTGCACCCGCT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MDELWKLPAHALTDGYATGRFTPQQALASCLARTAHCNPALNAVIAFDTEGAMAAAQESTARWRRGEPLGALDGVPVTVK
DNLHVRGLPTHWGSRALAGFVPDRDELPVARLRAAGALIFGKTNVPEFAMQGYTDNLVFGTTGNPWQPALTPGGSSGGAV
AAVASGCCPLALGTDGGGSIRRPASHTGLVGFKPSPGRLPRGDGLPEIFMRYEVVGPLARCVADAMAMAEALGQPDPRDP
DSRRFAGVPLQMPPRPCRVLHVPRFGDHPVDARIAALTGQAAQALRELGHTVDTAQAFDLAEATNAKWSLLSQAGLAWLV
EQGGAQLPFCPPLDAGTFGAATQANASAGAGLRAVQLFDLLMAVERLRSELSLLFARYDLLLTPAAAALPWPAAQSHPPV
IEGREAGPRGHAVFTAFANAAGLPAISLPCGEAEGLPVGLQLVAAPAEDALLLAIARQFEQAFPRAPGFPLDGQGR

Sequences:

>Translated_476_residues
MDELWKLPAHALTDGYATGRFTPQQALASCLARTAHCNPALNAVIAFDTEGAMAAAQESTARWRRGEPLGALDGVPVTVK
DNLHVRGLPTHWGSRALAGFVPDRDELPVARLRAAGALIFGKTNVPEFAMQGYTDNLVFGTTGNPWQPALTPGGSSGGAV
AAVASGCCPLALGTDGGGSIRRPASHTGLVGFKPSPGRLPRGDGLPEIFMRYEVVGPLARCVADAMAMAEALGQPDPRDP
DSRRFAGVPLQMPPRPCRVLHVPRFGDHPVDARIAALTGQAAQALRELGHTVDTAQAFDLAEATNAKWSLLSQAGLAWLV
EQGGAQLPFCPPLDAGTFGAATQANASAGAGLRAVQLFDLLMAVERLRSELSLLFARYDLLLTPAAAALPWPAAQSHPPV
IEGREAGPRGHAVFTAFANAAGLPAISLPCGEAEGLPVGLQLVAAPAEDALLLAIARQFEQAFPRAPGFPLDGQGR
>Mature_476_residues
MDELWKLPAHALTDGYATGRFTPQQALASCLARTAHCNPALNAVIAFDTEGAMAAAQESTARWRRGEPLGALDGVPVTVK
DNLHVRGLPTHWGSRALAGFVPDRDELPVARLRAAGALIFGKTNVPEFAMQGYTDNLVFGTTGNPWQPALTPGGSSGGAV
AAVASGCCPLALGTDGGGSIRRPASHTGLVGFKPSPGRLPRGDGLPEIFMRYEVVGPLARCVADAMAMAEALGQPDPRDP
DSRRFAGVPLQMPPRPCRVLHVPRFGDHPVDARIAALTGQAAQALRELGHTVDTAQAFDLAEATNAKWSLLSQAGLAWLV
EQGGAQLPFCPPLDAGTFGAATQANASAGAGLRAVQLFDLLMAVERLRSELSLLFARYDLLLTPAAAALPWPAAQSHPPV
IEGREAGPRGHAVFTAFANAAGLPAISLPCGEAEGLPVGLQLVAAPAEDALLLAIARQFEQAFPRAPGFPLDGQGR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=488, Percent_Identity=31.1475409836066, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI195972892, Length=468, Percent_Identity=27.1367521367521, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI166795287, Length=205, Percent_Identity=34.6341463414634, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=24.750499001996, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556264, Length=202, Percent_Identity=33.6633663366337, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=223, Percent_Identity=32.2869955156951, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=206, Percent_Identity=33.0097087378641, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=170, Percent_Identity=36.4705882352941, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=204, Percent_Identity=32.3529411764706, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=204, Percent_Identity=32.3529411764706, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=197, Percent_Identity=31.9796954314721, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=402, Percent_Identity=29.1044776119403, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=26.6509433962264, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320448, Length=184, Percent_Identity=35.8695652173913, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648113, Length=487, Percent_Identity=30.1848049281314, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24648435, Length=494, Percent_Identity=26.1133603238866, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648437, Length=494, Percent_Identity=26.1133603238866, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550774, Length=494, Percent_Identity=26.1133603238866, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648441, Length=422, Percent_Identity=25.8293838862559, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648439, Length=422, Percent_Identity=25.8293838862559, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24652985, Length=480, Percent_Identity=27.2916666666667, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19922090, Length=480, Percent_Identity=27.2916666666667, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24652981, Length=480, Percent_Identity=27.2916666666667, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24652983, Length=480, Percent_Identity=27.2916666666667, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24644968, Length=499, Percent_Identity=25.6513026052104, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21356731, Length=471, Percent_Identity=25.0530785562633, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078093, Length=495, Percent_Identity=25.0505050505051, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49585; Mature: 49585

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELWKLPAHALTDGYATGRFTPQQALASCLARTAHCNPALNAVIAFDTEGAMAAAQEST
CCHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
ARWRRGEPLGALDGVPVTVKDNLHVRGLPTHWGSRALAGFVPDRDELPVARLRAAGALIF
HHHHCCCCCCCCCCCCEEEECCEEEEECCCCCCCCHHCCCCCCCCCCCHHHHHHCCEEEE
GKTNVPEFAMQGYTDNLVFGTTGNPWQPALTPGGSSGGAVAAVASGCCPLALGTDGGGSI
CCCCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCC
RRPASHTGLVGFKPSPGRLPRGDGLPEIFMRYEVVGPLARCVADAMAMAEALGQPDPRDP
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DSRRFAGVPLQMPPRPCRVLHVPRFGDHPVDARIAALTGQAAQALRELGHTVDTAQAFDL
CCCCEECCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
AEATNAKWSLLSQAGLAWLVEQGGAQLPFCPPLDAGTFGAATQANASAGAGLRAVQLFDL
HHHCCCHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMAVERLRSELSLLFARYDLLLTPAAAALPWPAAQSHPPVIEGREAGPRGHAVFTAFANA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCC
AGLPAISLPCGEAEGLPVGLQLVAAPAEDALLLAIARQFEQAFPRAPGFPLDGQGR
CCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDELWKLPAHALTDGYATGRFTPQQALASCLARTAHCNPALNAVIAFDTEGAMAAAQEST
CCHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
ARWRRGEPLGALDGVPVTVKDNLHVRGLPTHWGSRALAGFVPDRDELPVARLRAAGALIF
HHHHCCCCCCCCCCCCEEEECCEEEEECCCCCCCCHHCCCCCCCCCCCHHHHHHCCEEEE
GKTNVPEFAMQGYTDNLVFGTTGNPWQPALTPGGSSGGAVAAVASGCCPLALGTDGGGSI
CCCCCCHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCC
RRPASHTGLVGFKPSPGRLPRGDGLPEIFMRYEVVGPLARCVADAMAMAEALGQPDPRDP
CCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DSRRFAGVPLQMPPRPCRVLHVPRFGDHPVDARIAALTGQAAQALRELGHTVDTAQAFDL
CCCCEECCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
AEATNAKWSLLSQAGLAWLVEQGGAQLPFCPPLDAGTFGAATQANASAGAGLRAVQLFDL
HHHCCCHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LMAVERLRSELSLLFARYDLLLTPAAAALPWPAAQSHPPVIEGREAGPRGHAVFTAFANA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCC
AGLPAISLPCGEAEGLPVGLQLVAAPAEDALLLAIARQFEQAFPRAPGFPLDGQGR
CCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA