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The map label for this gene is fadA [H]

Identifier: 116694710

GI number: 116694710

Start: 857318

End: 858493

Strand: Direct

Name: fadA [H]

Synonym: H16_B0759

Alternate gene names: 116694710

Gene position: 857318-858493 (Clockwise)

Preceding gene: 116694709

Following gene: 116694715

Centisome position: 29.44

GC content: 68.62

Gene sequence:

>1176_bases
ATGGCAGAGGCATATATCGTCGCGGCGGTCCGTACCGCCGGCGGCCGCAAGGGCGGCAAGCTGTCGGGCTGGCATCCGGC
CGACCTGGCCGCACAGGTGCTGGACGCACTGGTGGAACGCACCGGCGCCGACCCGGCGCTGGTCGAAGACGTCATCATGG
GCTGCGTGAGCCAGGTCGGCGAGCAGGCCGGCAACGTGGCGCGCAATGCCATCCTGGCCTCGCGCCTGCCTGAAAGCGTG
CCCGGCACCTCGGTGGACCGCCAGTGCGGTTCGTCGCAGCAGGCACTGCACTTTGCCGCGCAGGCAGTGATGTCGGGCGC
GATGGACATCGTCATCGCCGCCGGCGTGGAAAGCATGACGCGCGTGCCGATGGGCCTGTCGTCGCAGCTGCCGGCCAAGA
ACGGTTTCGGTGTGCCCAAGAGCCCCGGCATCGAGGCGCGCTATCCCGGCGTGCAGTTCAGCCAGTTCACCGGCGCCGAA
ATGATTGCGCGCAAGTACGACCTGTCGCGCGAGCAGCTGGATGCCTACGCCCTGCAGAGCCACCAGCGCGCCATCGCCGC
CACCAAGTCGGGCCGCTTCACCGCCGAAATCCTGCCGGTGGAAGTGCGCACGGCCGACGGTGCCAACGGTGAAATGCACA
CCACTGACGAAGGCGTCCGCTATGACGCCACGCTGGAAAGCATCGGCAGCGTCAAGCTGATCGCCGAAGGCGGCCGCGTG
ACTGCCGCTTCCGCCAGCCAGATCTGCGACGGCGCCGCCGGCCTGATGGTGGTCAACGAGGCCGGACTGAAGAAGCTTGG
CGTCAAGCCGCTGGCGCGCGTGCACGCCATGACGGTGATCGGCCATGATCCGGTCGTGATGCTGGAAGCGCCGCTGCCGG
CCACGGAAGTCGCGCTGAAGAAGGCGGGCCTGCGCATCGGCGATATCGACCTGTTCGAAGTCAACGAAGCCTTCGCCCCG
GTGCCGCTGGCCTGGCTGAAGGCCACCGGCGCCGATCCGGCGCGCCTGAACGTGCATGGCGGCGCGATCGCGCTGGGCCA
TCCGCTGGGCGGCTCGGGCGCCAAGCTGATGACCACGCTGGTACATGCGCTGCATACGCACGGCAAGCGCTACGGCCTGC
AGACGATGTGCGAAGGCGGCGGGCTGGCCAACGTGACCATCGTCGAGCGCCTGTAA

Upstream 100 bases:

>100_bases
GCGGCGCAACAATGCAAAGCGACACTGCCGGCGCCAGATGCCGGCACTCCGGACAACACCATGGCTGCCCCGGCAGCCTG
CACCGAACCAAGGAGATCAC

Downstream 100 bases:

>100_bases
AGCTGTGAGAGTCGCCGCGTGCCGCACAGGCACGCAGCGCATGGATCGCCGCAGCAAGGAGTAGTAAAGGGAGAAGGACC
GGCGGCGCGGGCATGTCAGA

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAEAYIVAAVRTAGGRKGGKLSGWHPADLAAQVLDALVERTGADPALVEDVIMGCVSQVGEQAGNVARNAILASRLPESV
PGTSVDRQCGSSQQALHFAAQAVMSGAMDIVIAAGVESMTRVPMGLSSQLPAKNGFGVPKSPGIEARYPGVQFSQFTGAE
MIARKYDLSREQLDAYALQSHQRAIAATKSGRFTAEILPVEVRTADGANGEMHTTDEGVRYDATLESIGSVKLIAEGGRV
TAASASQICDGAAGLMVVNEAGLKKLGVKPLARVHAMTVIGHDPVVMLEAPLPATEVALKKAGLRIGDIDLFEVNEAFAP
VPLAWLKATGADPARLNVHGGAIALGHPLGGSGAKLMTTLVHALHTHGKRYGLQTMCEGGGLANVTIVERL

Sequences:

>Translated_391_residues
MAEAYIVAAVRTAGGRKGGKLSGWHPADLAAQVLDALVERTGADPALVEDVIMGCVSQVGEQAGNVARNAILASRLPESV
PGTSVDRQCGSSQQALHFAAQAVMSGAMDIVIAAGVESMTRVPMGLSSQLPAKNGFGVPKSPGIEARYPGVQFSQFTGAE
MIARKYDLSREQLDAYALQSHQRAIAATKSGRFTAEILPVEVRTADGANGEMHTTDEGVRYDATLESIGSVKLIAEGGRV
TAASASQICDGAAGLMVVNEAGLKKLGVKPLARVHAMTVIGHDPVVMLEAPLPATEVALKKAGLRIGDIDLFEVNEAFAP
VPLAWLKATGADPARLNVHGGAIALGHPLGGSGAKLMTTLVHALHTHGKRYGLQTMCEGGGLANVTIVERL
>Mature_390_residues
AEAYIVAAVRTAGGRKGGKLSGWHPADLAAQVLDALVERTGADPALVEDVIMGCVSQVGEQAGNVARNAILASRLPESVP
GTSVDRQCGSSQQALHFAAQAVMSGAMDIVIAAGVESMTRVPMGLSSQLPAKNGFGVPKSPGIEARYPGVQFSQFTGAEM
IARKYDLSREQLDAYALQSHQRAIAATKSGRFTAEILPVEVRTADGANGEMHTTDEGVRYDATLESIGSVKLIAEGGRVT
AASASQICDGAAGLMVVNEAGLKKLGVKPLARVHAMTVIGHDPVVMLEAPLPATEVALKKAGLRIGDIDLFEVNEAFAPV
PLAWLKATGADPARLNVHGGAIALGHPLGGSGAKLMTTLVHALHTHGKRYGLQTMCEGGGLANVTIVERL

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=37.5321336760925, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=35.0125944584383, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=36.5728900255754, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=34.9246231155779, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4504327, Length=432, Percent_Identity=30.5555555555556, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=29.0076335877863, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=41.4572864321608, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=41.5422885572139, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=39.5, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=40.25, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1788683, Length=415, Percent_Identity=30.6024096385542, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=37.9134860050891, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17535921, Length=409, Percent_Identity=33.0073349633252, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI25147385, Length=396, Percent_Identity=33.5858585858586, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=29.9065420560748, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=394, Percent_Identity=29.1878172588832, Blast_Score=144, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=35.2357320099256, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6322031, Length=393, Percent_Identity=34.0966921119593, Blast_Score=178, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24655093, Length=394, Percent_Identity=37.3096446700508, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI17648125, Length=384, Percent_Identity=36.1979166666667, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24640423, Length=395, Percent_Identity=34.4303797468354, Blast_Score=205, Evalue=4e-53,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=28.169014084507, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40516; Mature: 40385

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYIVAAVRTAGGRKGGKLSGWHPADLAAQVLDALVERTGADPALVEDVIMGCVSQVG
CCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EQAGNVARNAILASRLPESVPGTSVDRQCGSSQQALHFAAQAVMSGAMDIVIAAGVESMT
HHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCHHEEEECCHHHHH
RVPMGLSSQLPAKNGFGVPKSPGIEARYPGVQFSQFTGAEMIARKYDLSREQLDAYALQS
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHCCCHHHHHHHHHHH
HQRAIAATKSGRFTAEILPVEVRTADGANGEMHTTDEGVRYDATLESIGSVKLIAEGGRV
HHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEECHHHHCCCCEEEEECCCEE
TAASASQICDGAAGLMVVNEAGLKKLGVKPLARVHAMTVIGHDPVVMLEAPLPATEVALK
EECCHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHEEEEECCCCEEEEECCCCHHHHHHH
KAGLRIGDIDLFEVNEAFAPVPLAWLKATGADPARLNVHGGAIALGHPLGGSGAKLMTTL
HCCCEECCEEEEECCCCCCCCCHHHHHCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHH
VHALHTHGKRYGLQTMCEGGGLANVTIVERL
HHHHHHCCHHCCHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure 
AEAYIVAAVRTAGGRKGGKLSGWHPADLAAQVLDALVERTGADPALVEDVIMGCVSQVG
CCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EQAGNVARNAILASRLPESVPGTSVDRQCGSSQQALHFAAQAVMSGAMDIVIAAGVESMT
HHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCHHEEEECCHHHHH
RVPMGLSSQLPAKNGFGVPKSPGIEARYPGVQFSQFTGAEMIARKYDLSREQLDAYALQS
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHHHCCCHHHHHHHHHHH
HQRAIAATKSGRFTAEILPVEVRTADGANGEMHTTDEGVRYDATLESIGSVKLIAEGGRV
HHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEECHHHHCCCCEEEEECCCEE
TAASASQICDGAAGLMVVNEAGLKKLGVKPLARVHAMTVIGHDPVVMLEAPLPATEVALK
EECCHHHHHCCCCCEEEEECCCHHHHCCCHHHHHHEEEEECCCCEEEEECCCCHHHHHHH
KAGLRIGDIDLFEVNEAFAPVPLAWLKATGADPARLNVHGGAIALGHPLGGSGAKLMTTL
HCCCEECCEEEEECCCCCCCCCHHHHHCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHH
VHALHTHGKRYGLQTMCEGGGLANVTIVERL
HHHHHHCCHHCCHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]