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The map label for this gene is pcaR [H]

Identifier: 116694698

GI number: 116694698

Start: 842221

End: 843072

Strand: Direct

Name: pcaR [H]

Synonym: H16_B0747

Alternate gene names: 116694698

Gene position: 842221-843072 (Clockwise)

Preceding gene: 116694697

Following gene: 116694699

Centisome position: 28.92

GC content: 68.9

Gene sequence:

>852_bases
ATGACGACCGATTCGCCGCAGGAAGACACGCAGGAAAGCGGCCAGGAGGGCGCCCCGGAGAGCGCCCAGGAGAAGTACAT
CGTGCCGGGGCTGGAGCGCGGCCTGCGGCTGCTGGCCGAATTCAGCAGCCGTGAGCGCACCTTGTCGGCGGCCGAGCTGG
CGCGCCGGCTCAAGGTGCCGCGTTCGACCGTGTTCCGGCTGCTGGCCACGCTGGAAATGATGGGCTTTGTCGAGCGCACC
GACGGCGGGCGCGAATTCCGCCTGGGCATGGCGGTGCTGCGGCTGGGCTTTGACTACCTGGCCTCGCTGGAGCTGACCGA
GCTGGGCCGCCCGCTGCTGGACCGGCTGCGCGACGAGATCCACTATCCGTGCAACCTGGTGGTGCGCGACGGGCGCTCCA
TCGTCTATGTGGCCAAGTCGGTGGCCTCGCGCCCGTTTGCCAGCACCGTCAACGTTGGCACCCGGCTGCCGGCGCATGCG
ACCGTGCTGGGCCGCGTGCTGCTGGAAGACCTGTCGCTGGCGGAACTGCGCACGCTGTATCCGGAAGAGCGCCTGGAGGT
GTATTCCGAAAGCACGCCGCGCACTGTCGAAGAGCTGTACGACATGGTCCAGCGCGACCGCCAGCGCGGCTATGTGCTGC
ATGAAGGGTTCTTCGAGGCCAGCATCTCCACCATCGCGGCGCCGGTGCGCGACCGCAGCGGCAAGGTTGTGGCGGCGCTG
GGCGCGACCATCCCGGCCTCGCGCATCGATCCGGACCAGCTCGACAATGTGGTGGAACAGGTCAGGCGGGCGGCGGCCGA
GCTGTCGAGCCTGCTCGACTACCGGCCGGAGGTGCCGAAACCGTTCGCGTGA

Upstream 100 bases:

>100_bases
GGAGCCAGGGCGGGGTCTGCCGGCAATACGGGTACCCTACGGGGTGCAGGCGTGATATATGAGAGCGTTACGCCAAGACC
GAATATTGCAGAGACAGACC

Downstream 100 bases:

>100_bases
CGGCAGCGCGCGCCGCCAGCCATCCATTGGGAAGCAATATGCAGCAACGCAAGACATACAACAGGCCGCCGCGCCGGCGG
CAATGGCTGGCCGGCACGGC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTTDSPQEDTQESGQEGAPESAQEKYIVPGLERGLRLLAEFSSRERTLSAAELARRLKVPRSTVFRLLATLEMMGFVERT
DGGREFRLGMAVLRLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGRSIVYVAKSVASRPFASTVNVGTRLPAHA
TVLGRVLLEDLSLAELRTLYPEERLEVYSESTPRTVEELYDMVQRDRQRGYVLHEGFFEASISTIAAPVRDRSGKVVAAL
GATIPASRIDPDQLDNVVEQVRRAAAELSSLLDYRPEVPKPFA

Sequences:

>Translated_283_residues
MTTDSPQEDTQESGQEGAPESAQEKYIVPGLERGLRLLAEFSSRERTLSAAELARRLKVPRSTVFRLLATLEMMGFVERT
DGGREFRLGMAVLRLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGRSIVYVAKSVASRPFASTVNVGTRLPAHA
TVLGRVLLEDLSLAELRTLYPEERLEVYSESTPRTVEELYDMVQRDRQRGYVLHEGFFEASISTIAAPVRDRSGKVVAAL
GATIPASRIDPDQLDNVVEQVRRAAAELSSLLDYRPEVPKPFA
>Mature_282_residues
TTDSPQEDTQESGQEGAPESAQEKYIVPGLERGLRLLAEFSSRERTLSAAELARRLKVPRSTVFRLLATLEMMGFVERTD
GGREFRLGMAVLRLGFDYLASLELTELGRPLLDRLRDEIHYPCNLVVRDGRSIVYVAKSVASRPFASTVNVGTRLPAHAT
VLGRVLLEDLSLAELRTLYPEERLEVYSESTPRTVEELYDMVQRDRQRGYVLHEGFFEASISTIAAPVRDRSGKVVAALG
ATIPASRIDPDQLDNVVEQVRRAAAELSSLLDYRPEVPKPFA

Specific function: Positive regulator of all genes within the pca regulon, pcaBDC, pcaIJ and pcaF. Also required for the chemotactic response to aromatic compounds [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=28.6290322580645, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786715, Length=262, Percent_Identity=25.9541984732824, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082359, Length=254, Percent_Identity=26.3779527559055, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788131, Length=237, Percent_Identity=29.1139240506329, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788581, Length=258, Percent_Identity=26.7441860465116, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789999, Length=262, Percent_Identity=24.4274809160305, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012794
- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 31605; Mature: 31474

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDSPQEDTQESGQEGAPESAQEKYIVPGLERGLRLLAEFSSRERTLSAAELARRLKVP
CCCCCCCHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RSTVFRLLATLEMMGFVERTDGGREFRLGMAVLRLGFDYLASLELTELGRPLLDRLRDEI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
HYPCNLVVRDGRSIVYVAKSVASRPFASTVNVGTRLPAHATVLGRVLLEDLSLAELRTLY
CCCCEEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PEERLEVYSESTPRTVEELYDMVQRDRQRGYVLHEGFFEASISTIAAPVRDRSGKVVAAL
CHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GATIPASRIDPDQLDNVVEQVRRAAAELSSLLDYRPEVPKPFA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTDSPQEDTQESGQEGAPESAQEKYIVPGLERGLRLLAEFSSRERTLSAAELARRLKVP
CCCCCCHHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RSTVFRLLATLEMMGFVERTDGGREFRLGMAVLRLGFDYLASLELTELGRPLLDRLRDEI
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
HYPCNLVVRDGRSIVYVAKSVASRPFASTVNVGTRLPAHATVLGRVLLEDLSLAELRTLY
CCCCEEEEECCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PEERLEVYSESTPRTVEELYDMVQRDRQRGYVLHEGFFEASISTIAAPVRDRSGKVVAAL
CHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GATIPASRIDPDQLDNVVEQVRRAAAELSSLLDYRPEVPKPFA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8083169 [H]