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The map label for this gene is alsR [H]

Identifier: 116694659

GI number: 116694659

Start: 797144

End: 798034

Strand: Direct

Name: alsR [H]

Synonym: H16_B0708

Alternate gene names: 116694659

Gene position: 797144-798034 (Clockwise)

Preceding gene: 116694653

Following gene: 116694661

Centisome position: 27.37

GC content: 70.26

Gene sequence:

>891_bases
ATGGACTTCCGCCAGCTGCAGCAGTTCGTCGCCCTGGCCGAGACGGGCAACTTCCACCGCGCCGCTGAACGCCTGCACAT
GGCGCAGCCGCCGCTTTCCATTTCCATCCGCAAGCTCGAGGACCAGCTTGGCACGGCGCTGTTCGTGCGCACCTCGCGCG
GGGTGAGGCTGACGCAGGCGGGCGAGGCTGCGCTGCACGATGCGCGCCGTGCACTGTTCCATGCGCAGCAGGCACGTGCC
GCCGCGATCGCCGCGGGGCAGGGCGAGCGCGGCGCGCTGCGCATCGGCTTTATCGGCTCGGCGACGTATGCGCTGCTGCC
CAAGCTGATCCCGGCGTTCCGCGCGGCGCATCCCGGCATCGAGCTGATCCTGCATGAATCCACCACCGCCGCGATCCTGG
ACCGCCTGGAAAAGCACCAGATCGACGCCGGCCTGGTGCGCTTTCCGATCCTGAACACCGGCAACTATTCGCTGACGCCG
CTGGAAAACGACGTCTTTGTCGCGGCAGTCCCGGCTGACAGTCCGCTGGCAGGCGCAGACGCCATCCCGCTGCGCGCGCT
TGCGGACGAGCCCTTCATCATGTATCCGGCGGCGCAGGTGCCCAACCTGCACGCGGTGGCAATGCTGCTGTGCCAGCAGG
CGGGCTTTGTGCCGCGTGTCACGCAGGAGGCGGTGCAGGTGCAGACCCAGGTCAGCCTGGTCGAGAGCGGGCTTGGCGTG
GCCCTGGTGCCGAGCGTGGCGGCGCGCTACGCCAACCGGCGCGTGCGCTTCCTGCCATTGTCCAGCCCGCGGCCGGCCGG
GCGCATCGGCATTGCGCTGGCGGCGCGGCCCGATGACGGCGACCGCCAGGTGCAGCGCTTCCTGGCGGCGGCGCAGGCGG
CAGTGAACTGA

Upstream 100 bases:

>100_bases
TCCGATCTTGTTGACTGTGGGGCACAGGGTAGATCAGCAGGCGAGGCTTGGTCTAATATTAGTTTTTTAGAAATCGATAT
TCACAGGATATTAACGAGCC

Downstream 100 bases:

>100_bases
TGCGGGGTGGTGCGGCGCCTCAGGCGGCGCGCGGGCGCGGCGCGCGCGGCCGCACAGCCGGGGTCACGCTGGCGCGCTCC
AGGTTCACGCCCGGCAGCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Als operon regulatory protein [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDFRQLQQFVALAETGNFHRAAERLHMAQPPLSISIRKLEDQLGTALFVRTSRGVRLTQAGEAALHDARRALFHAQQARA
AAIAAGQGERGALRIGFIGSATYALLPKLIPAFRAAHPGIELILHESTTAAILDRLEKHQIDAGLVRFPILNTGNYSLTP
LENDVFVAAVPADSPLAGADAIPLRALADEPFIMYPAAQVPNLHAVAMLLCQQAGFVPRVTQEAVQVQTQVSLVESGLGV
ALVPSVAARYANRRVRFLPLSSPRPAGRIGIALAARPDDGDRQVQRFLAAAQAAVN

Sequences:

>Translated_296_residues
MDFRQLQQFVALAETGNFHRAAERLHMAQPPLSISIRKLEDQLGTALFVRTSRGVRLTQAGEAALHDARRALFHAQQARA
AAIAAGQGERGALRIGFIGSATYALLPKLIPAFRAAHPGIELILHESTTAAILDRLEKHQIDAGLVRFPILNTGNYSLTP
LENDVFVAAVPADSPLAGADAIPLRALADEPFIMYPAAQVPNLHAVAMLLCQQAGFVPRVTQEAVQVQTQVSLVESGLGV
ALVPSVAARYANRRVRFLPLSSPRPAGRIGIALAARPDDGDRQVQRFLAAAQAAVN
>Mature_296_residues
MDFRQLQQFVALAETGNFHRAAERLHMAQPPLSISIRKLEDQLGTALFVRTSRGVRLTQAGEAALHDARRALFHAQQARA
AAIAAGQGERGALRIGFIGSATYALLPKLIPAFRAAHPGIELILHESTTAAILDRLEKHQIDAGLVRFPILNTGNYSLTP
LENDVFVAAVPADSPLAGADAIPLRALADEPFIMYPAAQVPNLHAVAMLLCQQAGFVPRVTQEAVQVQTQVSLVESGLGV
ALVPSVAARYANRRVRFLPLSSPRPAGRIGIALAARPDDGDRQVQRFLAAAQAAVN

Specific function: Regulates the expression of the alsSD operon for acetoin biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=35.0943396226415, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790399, Length=279, Percent_Identity=34.0501792114695, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=32.8767123287671, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787879, Length=269, Percent_Identity=35.6877323420074, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788297, Length=256, Percent_Identity=31.640625, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI157672245, Length=253, Percent_Identity=32.0158102766798, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693105, Length=253, Percent_Identity=28.8537549407115, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790208, Length=257, Percent_Identity=31.5175097276265, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=25.9109311740891, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=26.1044176706827, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081904, Length=280, Percent_Identity=29.6428571428571, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=34.7517730496454, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787806, Length=250, Percent_Identity=25.2, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788296, Length=252, Percent_Identity=29.7619047619048, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786713, Length=250, Percent_Identity=26.4, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786401, Length=159, Percent_Identity=32.0754716981132, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994962, Length=145, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=121, Percent_Identity=35.5371900826446, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31711; Mature: 31711

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRQLQQFVALAETGNFHRAAERLHMAQPPLSISIRKLEDQLGTALFVRTSRGVRLTQA
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCEEEEEECCCEEEECC
GEAALHDARRALFHAQQARAAAIAAGQGERGALRIGFIGSATYALLPKLIPAFRAAHPGI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCE
ELILHESTTAAILDRLEKHQIDAGLVRFPILNTGNYSLTPLENDVFVAAVPADSPLAGAD
EEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCEEEEEECCCCCCCCCC
AIPLRALADEPFIMYPAAQVPNLHAVAMLLCQQAGFVPRVTQEAVQVQTQVSLVESGLGV
CCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
ALVPSVAARYANRRVRFLPLSSPRPAGRIGIALAARPDDGDRQVQRFLAAAQAAVN
EEHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFRQLQQFVALAETGNFHRAAERLHMAQPPLSISIRKLEDQLGTALFVRTSRGVRLTQA
CCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCEEEEEECCCEEEECC
GEAALHDARRALFHAQQARAAAIAAGQGERGALRIGFIGSATYALLPKLIPAFRAAHPGI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCE
ELILHESTTAAILDRLEKHQIDAGLVRFPILNTGNYSLTPLENDVFVAAVPADSPLAGAD
EEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCCCEEEEEECCCCCCCCCC
AIPLRALADEPFIMYPAAQVPNLHAVAMLLCQQAGFVPRVTQEAVQVQTQVSLVESGLGV
CCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCE
ALVPSVAARYANRRVRFLPLSSPRPAGRIGIALAARPDDGDRQVQRFLAAAQAAVN
EEHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377 [H]