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The map label for this gene is echA8 [H]

Identifier: 116694610

GI number: 116694610

Start: 733379

End: 734170

Strand: Direct

Name: echA8 [H]

Synonym: H16_B0659

Alternate gene names: 116694610

Gene position: 733379-734170 (Clockwise)

Preceding gene: 116694609

Following gene: 116694611

Centisome position: 25.18

GC content: 68.81

Gene sequence:

>792_bases
ATGCAAACGAACAAAAAGACCGGGCCGTTCCGCATCGACACCGCCGATGGCATCGCCGAACTGGTGATCGACCATGCGCC
AGTCAATGCGCTCGACTGCGCCGGCTGGCATGCGCTGGCCGCCGCGATCGACCAGCTGGGCACCGATCCCGCCGTGCGCG
TGATCGTGCTGCGCGGCGAAGGCCGCGGCTTCTGCGCCGGCGTCGACATCAAGGAGCTGGCCGCGCACCCGCAGCGCATC
GTCGATGTCAACGCCGGTAACTACGCCACCTTCCGCGCCGTGCACCGCAATCCCAAGCCGGTCATCGTGGCCGTGCACGG
CTTCGTGCTGGGCGGCGGCATCGGCATCTGCGGCGCGGCCGACATCATCGTGGCCGCAGATTGTGCGCGCTTTGGCGTGC
CGGAGATCGACCGCGGCGCCATGGGCGGCGGCGCCCACCTGCAGCGCATGTTCGGCGTGCAGAAGGTCAGGGCGATGTAC
TTCACCGGCGAGATGATCGATGCCGCCGAGGCGTACCGGCTGGGCGCGGTCGAGCGCGTGGTGCCGCGCGCAGAGTTGCG
CGAGGCCGCCATGACGCTGGCCCGCCAGATCGCCGCCAAGAGCCCGGCCATGCTGCAGCTGGCCAAGGAAGCGCTCAACG
GCGTGGAAGACGGCAACTTGGAGGACAAATACCGCTGGGAACAGGGCTTCACGCTGCAGGCCTACATGAGCGCCGATTCC
GGCGAGGCGCGCGCCGCCTTTGTCGAAGGCCGCGAAGCCCGCTTTGCGCAGGGAGAGCGCGATGCAGCTTGA

Upstream 100 bases:

>100_bases
CAGATCGTGGCCGGCATGGCCGCGCAGGCACAGGCGCGCCTGGCGGCGCTGGCTGGCGCCGGACTGGCTGCAGCGGCCTG
ACCCACAGACAGAGGAAACC

Downstream 100 bases:

>100_bases
ATACACCGACGCCCAGCGCGCCTTCCGCGCCGAGGTGCGCGACTGGATGGCCGCGCACGTGCCGCGCACGCCGCTGGCGA
GCTTCGATACCGAAGCGGGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MQTNKKTGPFRIDTADGIAELVIDHAPVNALDCAGWHALAAAIDQLGTDPAVRVIVLRGEGRGFCAGVDIKELAAHPQRI
VDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAADIIVAADCARFGVPEIDRGAMGGGAHLQRMFGVQKVRAMY
FTGEMIDAAEAYRLGAVERVVPRAELREAAMTLARQIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSADS
GEARAAFVEGREARFAQGERDAA

Sequences:

>Translated_263_residues
MQTNKKTGPFRIDTADGIAELVIDHAPVNALDCAGWHALAAAIDQLGTDPAVRVIVLRGEGRGFCAGVDIKELAAHPQRI
VDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAADIIVAADCARFGVPEIDRGAMGGGAHLQRMFGVQKVRAMY
FTGEMIDAAEAYRLGAVERVVPRAELREAAMTLARQIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSADS
GEARAAFVEGREARFAQGERDAA
>Mature_263_residues
MQTNKKTGPFRIDTADGIAELVIDHAPVNALDCAGWHALAAAIDQLGTDPAVRVIVLRGEGRGFCAGVDIKELAAHPQRI
VDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAADIIVAADCARFGVPEIDRGAMGGGAHLQRMFGVQKVRAMY
FTGEMIDAAEAYRLGAVERVVPRAELREAAMTLARQIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSADS
GEARAAFVEGREARFAQGERDAA

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=233, Percent_Identity=33.9055793991416, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI70995211, Length=243, Percent_Identity=30.8641975308642, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=29.718875502008, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI4502327, Length=244, Percent_Identity=31.1475409836066, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI37594471, Length=220, Percent_Identity=28.6363636363636, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI37594469, Length=233, Percent_Identity=28.3261802575107, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI68989263, Length=177, Percent_Identity=27.683615819209, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI221142681, Length=243, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787659, Length=233, Percent_Identity=35.1931330472103, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=29.4339622641509, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87082183, Length=236, Percent_Identity=28.8135593220339, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787660, Length=226, Percent_Identity=30.5309734513274, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=34.5549738219895, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=31.413612565445, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=232, Percent_Identity=34.4827586206897, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17536985, Length=250, Percent_Identity=30, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17540714, Length=179, Percent_Identity=36.8715083798883, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25145438, Length=253, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=237, Percent_Identity=27.4261603375527, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=213, Percent_Identity=29.1079812206573, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=25.9109311740891, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=167, Percent_Identity=28.1437125748503, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=27.0718232044199, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=236, Percent_Identity=36.864406779661, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24653477, Length=236, Percent_Identity=36.864406779661, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19920382, Length=220, Percent_Identity=32.7272727272727, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653139, Length=227, Percent_Identity=32.5991189427313, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922422, Length=250, Percent_Identity=25.2, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650670, Length=218, Percent_Identity=31.651376146789, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21357171, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28013; Mature: 28013

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNKKTGPFRIDTADGIAELVIDHAPVNALDCAGWHALAAAIDQLGTDPAVRVIVLRGE
CCCCCCCCCEEEECHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHCCCCEEEEEEEECC
GRGFCAGVDIKELAAHPQRIVDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAA
CCEEEECCCHHHHHCCCCEEEEECCCCEEEEEEECCCCCCEEEEEECEEEECCCCCCCHH
DIIVAADCARFGVPEIDRGAMGGGAHLQRMFGVQKVRAMYFTGEMIDAAEAYRLGAVERV
HHHEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VPRAELREAAMTLARQIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSADS
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECCCC
GEARAAFVEGREARFAQGERDAA
CCCEEEECCCCHHHHCCCCCCCC
>Mature Secondary Structure
MQTNKKTGPFRIDTADGIAELVIDHAPVNALDCAGWHALAAAIDQLGTDPAVRVIVLRGE
CCCCCCCCCEEEECHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHCCCCEEEEEEEECC
GRGFCAGVDIKELAAHPQRIVDVNAGNYATFRAVHRNPKPVIVAVHGFVLGGGIGICGAA
CCEEEECCCHHHHHCCCCEEEEECCCCEEEEEEECCCCCCEEEEEECEEEECCCCCCCHH
DIIVAADCARFGVPEIDRGAMGGGAHLQRMFGVQKVRAMYFTGEMIDAAEAYRLGAVERV
HHHEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VPRAELREAAMTLARQIAAKSPAMLQLAKEALNGVEDGNLEDKYRWEQGFTLQAYMSADS
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEEECCCC
GEARAAFVEGREARFAQGERDAA
CCCEEEECCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]