| Definition | Ralstonia eutropha H16 chromosome 2, complete sequence. |
|---|---|
| Accession | NC_008314 |
| Length | 2,912,490 |
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The map label for this gene is yohF [C]
Identifier: 116694600
GI number: 116694600
Start: 723691
End: 724449
Strand: Direct
Name: yohF [C]
Synonym: H16_B0649
Alternate gene names: 116694600
Gene position: 723691-724449 (Clockwise)
Preceding gene: 116694599
Following gene: 116694603
Centisome position: 24.85
GC content: 66.67
Gene sequence:
>759_bases ATGCATCAAGTCAGGGACAAGGTTGCGCTGGTCACCGGCGCGGCCAGCGGGGTAGGGCGCGCCGACGCGCTGATGCTGGC GGCCGAAGGCGCGCGCGTGGTCATCAGCGATGTCAACCAGGAGGCCGGGCAGGCACTGGCCCGCGAGATCGGGGATGCGG CCATGTTCGTGCGCCACGACATTGCCAGCGAGGAGGACTGGCGCAAAGCCATGGCCGCCACGCAGGACCGCTTCGGCCGG CTCGACGTGCTGGTCAACAATGCGGCCATCTGTCCGCTCGGGTCAATCGAGGAAACCTCGCTCGACAGCTGGCGGCAGGT CATGCGCGTCAATGCCGACGGCTATTTCCTGGGCTGCAGGTTCGGTGTGCAGGCAATGAAGGGCAACCCCGCCGGGGGCT CCATCGTGGTGATGTCGTCGGTGGCGGCGCTGGGCGGGCTGCCCATGATGTGCGCATACAGCGGCGCCAAGGGCGCCGTT ACCGCGCTGACGCGCAGCGTGGCCGTGCACTGCAAGCAGAACGGCTACCGGATCCGCTGCAACTCGATCCATCCCGATGG CGTCTGGACGCCGATGACGCAGGCGCTGGTGCCGGACCTGGATCCGGCCACGCTTGGTATCGGTACGGACCCGATGGCAC GCATGTGCCTGCCGGAGGATGTGGCCAACCTGGTCCTGTTCCTGGCTTCGGACGCGTCGCGCTTTATCAATGGCGCGGAA TTGCGCATCGATAACGCGCAGTTGATCTCGGGGCTGTAA
Upstream 100 bases:
>100_bases GAAACCGTGGCTGCGGTGTATGGCGAAGCCGCGCTGCAGGCCGTGCTGGCCGGCAGGATTCCCGAAGCCGGCTGAAGCCG GCAACTCAAGGAGAGAAACG
Downstream 100 bases:
>100_bases GGCCCGCTGCGGCGGCCCGCGCAAGCAGGCTGCCGCAGCGCCGGGCTAACTGTACTGCGTCACGATCTCGCCGACCGTGC GCAGGATGTCGCGGCGGCGA
Product: 3-Beta-hydroxysteroid dehydrogenase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 252; Mature: 252
Protein sequence:
>252_residues MHQVRDKVALVTGAASGVGRADALMLAAEGARVVISDVNQEAGQALAREIGDAAMFVRHDIASEEDWRKAMAATQDRFGR LDVLVNNAAICPLGSIEETSLDSWRQVMRVNADGYFLGCRFGVQAMKGNPAGGSIVVMSSVAALGGLPMMCAYSGAKGAV TALTRSVAVHCKQNGYRIRCNSIHPDGVWTPMTQALVPDLDPATLGIGTDPMARMCLPEDVANLVLFLASDASRFINGAE LRIDNAQLISGL
Sequences:
>Translated_252_residues MHQVRDKVALVTGAASGVGRADALMLAAEGARVVISDVNQEAGQALAREIGDAAMFVRHDIASEEDWRKAMAATQDRFGR LDVLVNNAAICPLGSIEETSLDSWRQVMRVNADGYFLGCRFGVQAMKGNPAGGSIVVMSSVAALGGLPMMCAYSGAKGAV TALTRSVAVHCKQNGYRIRCNSIHPDGVWTPMTQALVPDLDPATLGIGTDPMARMCLPEDVANLVLFLASDASRFINGAE LRIDNAQLISGL >Mature_252_residues MHQVRDKVALVTGAASGVGRADALMLAAEGARVVISDVNQEAGQALAREIGDAAMFVRHDIASEEDWRKAMAATQDRFGR LDVLVNNAAICPLGSIEETSLDSWRQVMRVNADGYFLGCRFGVQAMKGNPAGGSIVVMSSVAALGGLPMMCAYSGAKGAV TALTRSVAVHCKQNGYRIRCNSIHPDGVWTPMTQALVPDLDPATLGIGTDPMARMCLPEDVANLVLFLASDASRFINGAE LRIDNAQLISGL
Specific function: Unknown
COG id: COG1028
COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI59889578, Length=220, Percent_Identity=35.4545454545455, Blast_Score=116, Evalue=2e-26, Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=35.15625, Blast_Score=113, Evalue=2e-25, Organism=Homo sapiens, GI4758504, Length=256, Percent_Identity=31.640625, Blast_Score=99, Evalue=5e-21, Organism=Homo sapiens, GI31542939, Length=261, Percent_Identity=30.6513409961686, Blast_Score=98, Evalue=9e-21, Organism=Homo sapiens, GI32483357, Length=238, Percent_Identity=31.9327731092437, Blast_Score=97, Evalue=1e-20, Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=30.2040816326531, Blast_Score=94, Evalue=8e-20, Organism=Homo sapiens, GI83715985, Length=255, Percent_Identity=30.5882352941176, Blast_Score=92, Evalue=4e-19, Organism=Homo sapiens, GI19923817, Length=251, Percent_Identity=28.2868525896414, Blast_Score=90, Evalue=2e-18, Organism=Homo sapiens, GI224922801, Length=175, Percent_Identity=35.4285714285714, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI4504505, Length=235, Percent_Identity=31.4893617021277, Blast_Score=89, Evalue=4e-18, Organism=Homo sapiens, GI66933014, Length=250, Percent_Identity=31.6, Blast_Score=87, Evalue=1e-17, Organism=Homo sapiens, GI109715829, Length=239, Percent_Identity=29.7071129707113, Blast_Score=86, Evalue=3e-17, Organism=Homo sapiens, GI7705925, Length=236, Percent_Identity=30.0847457627119, Blast_Score=83, Evalue=3e-16, Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=28.8065843621399, Blast_Score=77, Evalue=1e-14, Organism=Homo sapiens, GI10190704, Length=250, Percent_Identity=28.8, Blast_Score=75, Evalue=5e-14, Organism=Homo sapiens, GI4503301, Length=261, Percent_Identity=26.0536398467433, Blast_Score=75, Evalue=8e-14, Organism=Homo sapiens, GI126723191, Length=186, Percent_Identity=31.7204301075269, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI126723750, Length=241, Percent_Identity=27.3858921161826, Blast_Score=74, Evalue=1e-13, Organism=Homo sapiens, GI33667109, Length=180, Percent_Identity=32.2222222222222, Blast_Score=68, Evalue=6e-12, Organism=Homo sapiens, GI20149619, Length=199, Percent_Identity=28.643216080402, Blast_Score=68, Evalue=7e-12, Organism=Homo sapiens, GI146231950, Length=216, Percent_Identity=29.6296296296296, Blast_Score=65, Evalue=4e-11, Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=29.3650793650794, Blast_Score=112, Evalue=3e-26, Organism=Escherichia coli, GI87082100, Length=254, Percent_Identity=33.0708661417323, Blast_Score=104, Evalue=5e-24, Organism=Escherichia coli, GI2367175, Length=242, Percent_Identity=32.6446280991736, Blast_Score=98, Evalue=4e-22, Organism=Escherichia coli, GI87082160, Length=252, Percent_Identity=30.1587301587302, Blast_Score=98, Evalue=5e-22, Organism=Escherichia coli, GI1790717, Length=247, Percent_Identity=30.3643724696356, Blast_Score=97, Evalue=8e-22, Organism=Escherichia coli, GI1787335, Length=238, Percent_Identity=28.9915966386555, Blast_Score=97, Evalue=1e-21, Organism=Escherichia coli, GI1787905, Length=244, Percent_Identity=29.9180327868852, Blast_Score=91, Evalue=1e-19, Organism=Escherichia coli, GI1789378, Length=248, Percent_Identity=27.8225806451613, Blast_Score=88, Evalue=5e-19, Organism=Escherichia coli, GI1789057, Length=260, Percent_Identity=28.4615384615385, Blast_Score=87, Evalue=1e-18, Organism=Escherichia coli, GI1789208, Length=248, Percent_Identity=29.0322580645161, Blast_Score=87, Evalue=2e-18, Organism=Escherichia coli, GI1786812, Length=252, Percent_Identity=31.3492063492063, Blast_Score=85, Evalue=4e-18, Organism=Escherichia coli, GI1787526, Length=245, Percent_Identity=28.1632653061224, Blast_Score=79, Evalue=3e-16, Organism=Escherichia coli, GI2367365, Length=245, Percent_Identity=28.1632653061224, Blast_Score=68, Evalue=5e-13, Organism=Caenorhabditis elegans, GI17538182, Length=257, Percent_Identity=33.852140077821, Blast_Score=112, Evalue=1e-25, Organism=Caenorhabditis elegans, GI17561402, Length=240, Percent_Identity=33.75, Blast_Score=106, Evalue=9e-24, Organism=Caenorhabditis elegans, GI17560676, Length=244, Percent_Identity=31.9672131147541, Blast_Score=101, Evalue=4e-22, Organism=Caenorhabditis elegans, GI17555706, Length=241, Percent_Identity=30.2904564315353, Blast_Score=100, Evalue=5e-22, Organism=Caenorhabditis elegans, GI25147288, Length=243, Percent_Identity=33.7448559670782, Blast_Score=100, Evalue=1e-21, Organism=Caenorhabditis elegans, GI17531453, Length=268, Percent_Identity=32.089552238806, Blast_Score=97, Evalue=7e-21, Organism=Caenorhabditis elegans, GI17559104, Length=272, Percent_Identity=30.5147058823529, Blast_Score=96, Evalue=2e-20, Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=30.188679245283, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17562910, Length=276, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=3e-19, Organism=Caenorhabditis elegans, GI71994604, Length=247, Percent_Identity=29.1497975708502, Blast_Score=91, Evalue=8e-19, Organism=Caenorhabditis elegans, GI193204405, Length=269, Percent_Identity=29.7397769516729, Blast_Score=87, Evalue=8e-18, Organism=Caenorhabditis elegans, GI17562908, Length=265, Percent_Identity=29.811320754717, Blast_Score=87, Evalue=1e-17, Organism=Caenorhabditis elegans, GI17551412, Length=263, Percent_Identity=29.6577946768061, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=28.2352941176471, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17560332, Length=270, Percent_Identity=30.7407407407407, Blast_Score=85, Evalue=3e-17, Organism=Caenorhabditis elegans, GI17567345, Length=193, Percent_Identity=31.0880829015544, Blast_Score=84, Evalue=7e-17, Organism=Caenorhabditis elegans, GI17562904, Length=270, Percent_Identity=28.8888888888889, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17536025, Length=255, Percent_Identity=27.843137254902, Blast_Score=82, Evalue=3e-16, Organism=Caenorhabditis elegans, GI17538480, Length=260, Percent_Identity=29.2307692307692, Blast_Score=81, Evalue=5e-16, Organism=Caenorhabditis elegans, GI71994600, Length=250, Percent_Identity=25.6, Blast_Score=79, Evalue=2e-15, Organism=Caenorhabditis elegans, GI17560220, Length=274, Percent_Identity=27.7372262773723, Blast_Score=78, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17565030, Length=269, Percent_Identity=30.1115241635688, Blast_Score=78, Evalue=4e-15, Organism=Caenorhabditis elegans, GI115534694, Length=252, Percent_Identity=30.5555555555556, Blast_Score=77, Evalue=7e-15, Organism=Caenorhabditis elegans, GI17563726, Length=254, Percent_Identity=26.7716535433071, Blast_Score=75, Evalue=2e-14, Organism=Caenorhabditis elegans, GI17560150, Length=266, Percent_Identity=27.4436090225564, Blast_Score=74, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17568967, Length=210, Percent_Identity=30.952380952381, Blast_Score=73, Evalue=1e-13, Organism=Caenorhabditis elegans, GI17544670, Length=268, Percent_Identity=29.1044776119403, Blast_Score=72, Evalue=3e-13, Organism=Caenorhabditis elegans, GI17564282, Length=241, Percent_Identity=27.8008298755187, Blast_Score=71, Evalue=5e-13, Organism=Caenorhabditis elegans, GI17538486, Length=209, Percent_Identity=30.1435406698565, Blast_Score=70, Evalue=1e-12, Organism=Caenorhabditis elegans, GI72000259, Length=276, Percent_Identity=28.6231884057971, Blast_Score=69, Evalue=2e-12, Organism=Caenorhabditis elegans, GI17562990, Length=247, Percent_Identity=27.9352226720648, Blast_Score=68, Evalue=4e-12, Organism=Saccharomyces cerevisiae, GI6322861, Length=243, Percent_Identity=28.3950617283951, Blast_Score=76, Evalue=6e-15, Organism=Saccharomyces cerevisiae, GI6324126, Length=250, Percent_Identity=23.6, Blast_Score=65, Evalue=1e-11, Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=35.546875, Blast_Score=118, Evalue=4e-27, Organism=Drosophila melanogaster, GI17737361, Length=254, Percent_Identity=34.6456692913386, Blast_Score=115, Evalue=3e-26, Organism=Drosophila melanogaster, GI28571526, Length=255, Percent_Identity=30.9803921568627, Blast_Score=103, Evalue=9e-23, Organism=Drosophila melanogaster, GI21355319, Length=245, Percent_Identity=29.7959183673469, Blast_Score=103, Evalue=9e-23, Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=32.8, Blast_Score=103, Evalue=9e-23, Organism=Drosophila melanogaster, GI24639444, Length=241, Percent_Identity=32.7800829875519, Blast_Score=101, Evalue=4e-22, Organism=Drosophila melanogaster, GI23397609, Length=247, Percent_Identity=30.3643724696356, Blast_Score=86, Evalue=2e-17, Organism=Drosophila melanogaster, GI18859837, Length=203, Percent_Identity=32.512315270936, Blast_Score=84, Evalue=8e-17, Organism=Drosophila melanogaster, GI24644337, Length=252, Percent_Identity=30.952380952381, Blast_Score=82, Evalue=4e-16, Organism=Drosophila melanogaster, GI24643142, Length=246, Percent_Identity=29.6747967479675, Blast_Score=78, Evalue=6e-15, Organism=Drosophila melanogaster, GI21355631, Length=168, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=6e-13, Organism=Drosophila melanogaster, GI28571387, Length=242, Percent_Identity=26.8595041322314, Blast_Score=70, Evalue=1e-12, Organism=Drosophila melanogaster, GI24641232, Length=238, Percent_Identity=29.4117647058824, Blast_Score=69, Evalue=2e-12, Organism=Drosophila melanogaster, GI24640492, Length=188, Percent_Identity=28.1914893617021, Blast_Score=69, Evalue=2e-12, Organism=Drosophila melanogaster, GI281364892, Length=193, Percent_Identity=28.4974093264249, Blast_Score=68, Evalue=6e-12, Organism=Drosophila melanogaster, GI24580925, Length=225, Percent_Identity=25.7777777777778, Blast_Score=67, Evalue=9e-12, Organism=Drosophila melanogaster, GI116007236, Length=234, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=5e-11, Organism=Drosophila melanogaster, GI19921754, Length=207, Percent_Identity=29.951690821256, Blast_Score=65, Evalue=6e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: =1.1.1.51 [H]
Molecular weight: Translated: 26617; Mature: 26617
Theoretical pI: Translated: 5.67; Mature: 5.67
Prosite motif: PS00061 ADH_SHORT
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.4 %Cys (Translated Protein) 4.8 %Met (Translated Protein) 7.1 %Cys+Met (Translated Protein) 2.4 %Cys (Mature Protein) 4.8 %Met (Mature Protein) 7.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MHQVRDKVALVTGAASGVGRADALMLAAEGARVVISDVNQEAGQALAREIGDAAMFVRHD CCCHHHHEEEEECCCCCCCCHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH IASEEDWRKAMAATQDRFGRLDVLVNNAAICPLGSIEETSLDSWRQVMRVNADGYFLGCR CCCCHHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCCHHHHHHHHEECCCCEEEEEE FGVQAMKGNPAGGSIVVMSSVAALGGLPMMCAYSGAKGAVTALTRSVAVHCKQNGYRIRC ECCEEECCCCCCCEEEEEEHHHHHCCCCCEEEECCCCHHHHHHHHHHHEEECCCCEEEEE NSIHPDGVWTPMTQALVPDLDPATLGIGTDPMARMCLPEDVANLVLFLASDASRFINGAE CCCCCCCCCCCHHHHHCCCCCCCEECCCCCHHHHHCCHHHHHHHHHHHHCCHHHHCCCCE LRIDNAQLISGL EEECCHHHHCCC >Mature Secondary Structure MHQVRDKVALVTGAASGVGRADALMLAAEGARVVISDVNQEAGQALAREIGDAAMFVRHD CCCHHHHEEEEECCCCCCCCHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH IASEEDWRKAMAATQDRFGRLDVLVNNAAICPLGSIEETSLDSWRQVMRVNADGYFLGCR CCCCHHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCCHHHHHHHHEECCCCEEEEEE FGVQAMKGNPAGGSIVVMSSVAALGGLPMMCAYSGAKGAVTALTRSVAVHCKQNGYRIRC ECCEEECCCCCCCEEEEEEHHHHHCCCCCEEEECCCCHHHHHHHHHHHEEECCCCEEEEE NSIHPDGVWTPMTQALVPDLDPATLGIGTDPMARMCLPEDVANLVLFLASDASRFINGAE CCCCCCCCCCCHHHHHCCCCCCCEECCCCCHHHHHCCHHHHHHHHHHHHCCHHHHCCCCE LRIDNAQLISGL EEECCHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 8382516; 2026158; 8617258 [H]