BacMap

Definition	Ralstonia eutropha H16 chromosome 2, complete sequence.
Accession	NC_008314
Length	2,912,490

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The map label for this gene is ykvO [H]

Identifier: 116694599

GI number: 116694599

Start: 722808

End: 723665

Strand: Direct

Name: ykvO [H]

Synonym: H16_B0648

Alternate gene names: 116694599

Gene position: 722808-723665 (Clockwise)

Preceding gene: 116694598

Following gene: 116694600

Centisome position: 24.82

GC content: 71.68

Gene sequence:

>858_bases
ATGATGACGACGACCCGTGTACTGGAAGGCAAGACAGCCCTAGTCACCGGCGGCGCCGGCGGCATCGGTGCGGCCAGCGC
GCTGGCGCTGCTGCGCGACGGCGCGACGGTGGTCCTCATGGGGCGGCGGGGCGAAGCGCTGGAAGCGGCACGGCGCAGCC
TGTGCGCGCAGGTGGCCGGCGCCACCGTGGCAATCAGCGTCGGCGACGCGTGCCGCGAGGATGACGTGCAGGCCGCGCTG
CAGCAAGCCTGGGAGCTGCAGCGGCGCCTCGATATCGTGGTCGCGACCGTTGGTGGGGGCGGCTTCCGTCCGCTGCTGAT
GCATGACGCGGCCTCGCTGATGGCGGAGCTGGAGCTGAACATCGCCAGCGCATTCCTGGCGGTGCGCCACGCGTCGCCGC
TGATGGCACCGCATGGCGGCACCATCGTCTGTATCTCGTCGAACGCGGCGCGCATGACCTGCCGGTGGCTGTCGGCGTAC
TGCGCCGCCAAGGCGGGGCTGGAAGCGTTCGTGCGCGCCGCGGCCGAGGAGCTGGCGGGATGCGGCATCCGCGTCAATGC
GGTGCGGCCGGGGCTAACCCGCTCCGGCGCGACCGGGCCGATGTTCGACAACCCGGCCATCCTCGACAAGTTCGTCGCGC
AGATGCCGCTGGGGCGCGCCGGCGAACCGGACGACATTGCCAGTGCGGTGCGCTACCTGGCGGGGCCGGAATCGGGCTGG
GTGACCGGCCAGAGCATCGCCGTGGACGGTGGCAGCGAGCTGCGCGGCAATCCCATCCTCGATGAAACCGTGGCTGCGGT
GTATGGCGAAGCCGCGCTGCAGGCCGTGCTGGCCGGCAGGATTCCCGAAGCCGGCTGA

Upstream 100 bases:

>100_bases
TCCGGTACCAGGACCGCTTCGTGCGGCGCGACGGCCAGTGGCGCATTGCCGCGCGCGAGCTGGTGGTGGACTGGACGCAG
GACCTGCCGCTGCGAGGGGC

Downstream 100 bases:

>100_bases
AGCCGGCAACTCAAGGAGAGAAACGATGCATCAAGTCAGGGACAAGGTTGCGCTGGTCACCGGCGCGGCCAGCGGGGTAG
GGCGCGCCGACGCGCTGATG

Product: 7-Alpha-hydroxysteroid dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MMTTTRVLEGKTALVTGGAGGIGAASALALLRDGATVVLMGRRGEALEAARRSLCAQVAGATVAISVGDACREDDVQAAL
QQAWELQRRLDIVVATVGGGGFRPLLMHDAASLMAELELNIASAFLAVRHASPLMAPHGGTIVCISSNAARMTCRWLSAY
CAAKAGLEAFVRAAAEELAGCGIRVNAVRPGLTRSGATGPMFDNPAILDKFVAQMPLGRAGEPDDIASAVRYLAGPESGW
VTGQSIAVDGGSELRGNPILDETVAAVYGEAALQAVLAGRIPEAG

Sequences:

>Translated_285_residues
MMTTTRVLEGKTALVTGGAGGIGAASALALLRDGATVVLMGRRGEALEAARRSLCAQVAGATVAISVGDACREDDVQAAL
QQAWELQRRLDIVVATVGGGGFRPLLMHDAASLMAELELNIASAFLAVRHASPLMAPHGGTIVCISSNAARMTCRWLSAY
CAAKAGLEAFVRAAAEELAGCGIRVNAVRPGLTRSGATGPMFDNPAILDKFVAQMPLGRAGEPDDIASAVRYLAGPESGW
VTGQSIAVDGGSELRGNPILDETVAAVYGEAALQAVLAGRIPEAG
>Mature_285_residues
MMTTTRVLEGKTALVTGGAGGIGAASALALLRDGATVVLMGRRGEALEAARRSLCAQVAGATVAISVGDACREDDVQAAL
QQAWELQRRLDIVVATVGGGGFRPLLMHDAASLMAELELNIASAFLAVRHASPLMAPHGGTIVCISSNAARMTCRWLSAY
CAAKAGLEAFVRAAAEELAGCGIRVNAVRPGLTRSGATGPMFDNPAILDKFVAQMPLGRAGEPDDIASAVRYLAGPESGW
VTGQSIAVDGGSELRGNPILDETVAAVYGEAALQAVLAGRIPEAG

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=255, Percent_Identity=30.5882352941176, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI5031737, Length=250, Percent_Identity=31.6, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI32483357, Length=256, Percent_Identity=29.296875, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40254992, Length=245, Percent_Identity=30.6122448979592, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI15277342, Length=259, Percent_Identity=30.8880308880309, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI31542939, Length=232, Percent_Identity=29.7413793103448, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI7705925, Length=253, Percent_Identity=26.0869565217391, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI10190704, Length=256, Percent_Identity=29.6875, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI126723750, Length=259, Percent_Identity=27.4131274131274, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4503301, Length=251, Percent_Identity=25.8964143426295, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4758504, Length=266, Percent_Identity=28.1954887218045, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI59889578, Length=247, Percent_Identity=27.9352226720648, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI33667109, Length=193, Percent_Identity=31.6062176165803, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87082160, Length=252, Percent_Identity=30.1587301587302, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI2367365, Length=247, Percent_Identity=33.6032388663968, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789378, Length=257, Percent_Identity=32.295719844358, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=30.5019305019305, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787335, Length=245, Percent_Identity=31.8367346938775, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786812, Length=259, Percent_Identity=30.1158301158301, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367175, Length=257, Percent_Identity=31.1284046692607, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789208, Length=265, Percent_Identity=28.6792452830189, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790717, Length=251, Percent_Identity=23.9043824701195, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788459, Length=257, Percent_Identity=29.5719844357977, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789057, Length=258, Percent_Identity=27.1317829457364, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=273, Percent_Identity=31.8681318681319, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17562904, Length=262, Percent_Identity=30.1526717557252, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560332, Length=259, Percent_Identity=32.4324324324324, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17531453, Length=262, Percent_Identity=30.1526717557252, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI72000259, Length=259, Percent_Identity=30.1158301158301, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538480, Length=262, Percent_Identity=31.2977099236641, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17562990, Length=265, Percent_Identity=29.811320754717, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17551412, Length=263, Percent_Identity=30.7984790874525, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560220, Length=261, Percent_Identity=29.1187739463602, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17563726, Length=260, Percent_Identity=29.6153846153846, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17560676, Length=254, Percent_Identity=29.1338582677165, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17561402, Length=267, Percent_Identity=29.9625468164794, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17562906, Length=262, Percent_Identity=30.5343511450382, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=29.3893129770992, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562908, Length=262, Percent_Identity=30.1526717557252, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI193204405, Length=265, Percent_Identity=29.811320754717, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17538486, Length=261, Percent_Identity=30.6513409961686, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25147288, Length=248, Percent_Identity=32.6612903225806, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17555706, Length=255, Percent_Identity=31.3725490196078, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17544670, Length=277, Percent_Identity=27.0758122743682, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560150, Length=268, Percent_Identity=27.9850746268657, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=246, Percent_Identity=29.2682926829268, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565030, Length=259, Percent_Identity=28.957528957529, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=248, Percent_Identity=26.6129032258064, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17538182, Length=271, Percent_Identity=27.3062730627306, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=248, Percent_Identity=27.8225806451613, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536651, Length=255, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193207185, Length=147, Percent_Identity=32.6530612244898, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=247, Percent_Identity=27.5303643724696, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323882, Length=198, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI23397609, Length=254, Percent_Identity=31.8897637795276, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21355319, Length=258, Percent_Identity=30.2325581395349, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=31.0756972111554, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21357041, Length=254, Percent_Identity=29.9212598425197, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28571526, Length=251, Percent_Identity=30.2788844621514, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643142, Length=246, Percent_Identity=30.0813008130081, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24644337, Length=255, Percent_Identity=30.9803921568627, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17737361, Length=260, Percent_Identity=29.2307692307692, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 29121; Mature: 29121

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTTRVLEGKTALVTGGAGGIGAASALALLRDGATVVLMGRRGEALEAARRSLCAQVAG
CCCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCC
ATVAISVGDACREDDVQAALQQAWELQRRLDIVVATVGGGGFRPLLMHDAASLMAELELN
CEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
IASAFLAVRHASPLMAPHGGTIVCISSNAARMTCRWLSAYCAAKAGLEAFVRAAAEELAG
HHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CGIRVNAVRPGLTRSGATGPMFDNPAILDKFVAQMPLGRAGEPDDIASAVRYLAGPESGW
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
VTGQSIAVDGGSELRGNPILDETVAAVYGEAALQAVLAGRIPEAG
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMTTTRVLEGKTALVTGGAGGIGAASALALLRDGATVVLMGRRGEALEAARRSLCAQVAG
CCCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCC
ATVAISVGDACREDDVQAALQQAWELQRRLDIVVATVGGGGFRPLLMHDAASLMAELELN
CEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
IASAFLAVRHASPLMAPHGGTIVCISSNAARMTCRWLSAYCAAKAGLEAFVRAAAEELAG
HHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
CGIRVNAVRPGLTRSGATGPMFDNPAILDKFVAQMPLGRAGEPDDIASAVRYLAGPESGW
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
VTGQSIAVDGGSELRGNPILDETVAAVYGEAALQAVLAGRIPEAG
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]