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The map label for this gene is fadA [C]

Identifier: 116694542

GI number: 116694542

Start: 664061

End: 665233

Strand: Direct

Name: fadA [C]

Synonym: H16_B0591

Alternate gene names: 116694542

Gene position: 664061-665233 (Clockwise)

Preceding gene: 116694541

Following gene: 116694551

Centisome position: 22.8

GC content: 65.64

Gene sequence:

>1173_bases
ATGAGTTTGTCGAATTTGCGTGGCAGCGTCGCCATCGTCGGGGTCGGCCAGGCGGGCCTGGGCGAGGCCCACGGCAAGAC
TGAAATGGAAATCCTGGTGGAGGCCGCCAAGGCCGCCGTCAGGGATGCGGGGCTGTCGATGCGCGACATCGACGGCATCG
CCACCGCCAGCGTCGGCGCCAGCATGTGGGTGATGCCGGTGGCCGAGTACCTTGGCATCAAGCCCAAGTTTGTCGACAGC
ACCATGATCGGAGGCTCCAGCTTTGTCTCGCACATGCTGCCGGCGATGATGGCGCTGGAATCGGGGCAGTGCGATGCGGT
GCTGGTCTGCTACGGCAGCAACCAGCGCACCTCCACGGTTGGCCGCGCCGGCATCTCCAAGGTGCGCGCGGTGCTGGACC
CGCAGCCGTATGAGCACCCGTACAGCCCGCTGCAGCCGATCACGTCATATGCTCTCGCCGCGGCGCGCCATATGCACCAG
TACGGCACCACGCGCGAGCACCTGGCCGAGGTCGCCGTGGCCGCGCGCAAGTGGGCGCAGCTGAATCCGGAAGCGATGAT
GCGAGACCCGCTCAGCATCGATGATGTGCTCAATGCGCGCATGGTGTCGGACCCGCTGTCCGTGCGTGACTGCTGCCTGG
TCACCGATGGCGCCGGCGCCTATGTGATGGTGCGCGCGGACCGCGCCAGGGACCTGGCCAAGCCGCCGGTCTATGTGCTG
GGCAATGCCACTGCCACGTGGCACCGGCAGATCTCGTGCATGCCGGACCTGACCGTCACGGCTGCCAAGGAATCCGGCGA
GCGCGCCTTTGCCATGGCGGGGCTCAAGCCGTCGGATGTCGATGTGGCCGAGCTCTATGATGCTTTCACCATCAACACGC
TGCTGTTCCTGGAAGACCTGGGTTTCTGCGCCAAGGGTGAGGGCGGCCAGTTTGTCAGCGGTGGCGGCATTGCGCCGGGC
GGGCGGCTGCCGGTGAATACCAATGGCGGTGGCTTGTCATGCGTGCATCCTGGGATGTATGGGATGTTCATCACCATTGA
GGCGGTGCGGCAGTTGCGTGGGGAATGTGGGGAGCGGCAGGTACGGGATGCCGAGGTTGCCGTGGTGCATGGGAATGGGG
GGACGTTGTCGAGTCAGGCTACGGCGATTCTGGGGACTGGGGCGACGGTTTAA

Upstream 100 bases:

>100_bases
GCCAGCGTGCGCATCGGCATGCGTGTGCAGGCACGCGTGGCGCAGGGCAAGGAAGGCGAGGCCGCTCTCGTCGTGTTCGA
TCCGGTGGAGGGCTGAGGCC

Downstream 100 bases:

>100_bases
GGCGGGAGCGGGGGGGCTATTGGCTTGGCGCCATGCGGGCCTGTCGCCCCCACCCCAACGCAGTTCAATCCCCCAACTCA
GGCCACCCCTGCCCGCCGCC

Product: thiolase

Products: CoA; oxoacyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSLSNLRGSVAIVGVGQAGLGEAHGKTEMEILVEAAKAAVRDAGLSMRDIDGIATASVGASMWVMPVAEYLGIKPKFVDS
TMIGGSSFVSHMLPAMMALESGQCDAVLVCYGSNQRTSTVGRAGISKVRAVLDPQPYEHPYSPLQPITSYALAAARHMHQ
YGTTREHLAEVAVAARKWAQLNPEAMMRDPLSIDDVLNARMVSDPLSVRDCCLVTDGAGAYVMVRADRARDLAKPPVYVL
GNATATWHRQISCMPDLTVTAAKESGERAFAMAGLKPSDVDVAELYDAFTINTLLFLEDLGFCAKGEGGQFVSGGGIAPG
GRLPVNTNGGGLSCVHPGMYGMFITIEAVRQLRGECGERQVRDAEVAVVHGNGGTLSSQATAILGTGATV

Sequences:

>Translated_390_residues
MSLSNLRGSVAIVGVGQAGLGEAHGKTEMEILVEAAKAAVRDAGLSMRDIDGIATASVGASMWVMPVAEYLGIKPKFVDS
TMIGGSSFVSHMLPAMMALESGQCDAVLVCYGSNQRTSTVGRAGISKVRAVLDPQPYEHPYSPLQPITSYALAAARHMHQ
YGTTREHLAEVAVAARKWAQLNPEAMMRDPLSIDDVLNARMVSDPLSVRDCCLVTDGAGAYVMVRADRARDLAKPPVYVL
GNATATWHRQISCMPDLTVTAAKESGERAFAMAGLKPSDVDVAELYDAFTINTLLFLEDLGFCAKGEGGQFVSGGGIAPG
GRLPVNTNGGGLSCVHPGMYGMFITIEAVRQLRGECGERQVRDAEVAVVHGNGGTLSSQATAILGTGATV
>Mature_389_residues
SLSNLRGSVAIVGVGQAGLGEAHGKTEMEILVEAAKAAVRDAGLSMRDIDGIATASVGASMWVMPVAEYLGIKPKFVDST
MIGGSSFVSHMLPAMMALESGQCDAVLVCYGSNQRTSTVGRAGISKVRAVLDPQPYEHPYSPLQPITSYALAAARHMHQY
GTTREHLAEVAVAARKWAQLNPEAMMRDPLSIDDVLNARMVSDPLSVRDCCLVTDGAGAYVMVRADRARDLAKPPVYVLG
NATATWHRQISCMPDLTVTAAKESGERAFAMAGLKPSDVDVAELYDAFTINTLLFLEDLGFCAKGEGGQFVSGGGIAPGG
RLPVNTNGGGLSCVHPGMYGMFITIEAVRQLRGECGERQVRDAEVAVVHGNGGTLSSQATAILGTGATV

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI302344767, Length=242, Percent_Identity=33.4710743801653, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI302344760, Length=242, Percent_Identity=33.4710743801653, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI19923233, Length=242, Percent_Identity=33.4710743801653, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI302344762, Length=242, Percent_Identity=33.4710743801653, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI55956775, Length=205, Percent_Identity=31.219512195122, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17537653, Length=235, Percent_Identity=31.9148936170213, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19921506, Length=232, Percent_Identity=33.1896551724138, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24585051, Length=232, Percent_Identity=32.7586206896552, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16 [C]

Molecular weight: Translated: 40973; Mature: 40842

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSNLRGSVAIVGVGQAGLGEAHGKTEMEILVEAAKAAVRDAGLSMRDIDGIATASVGA
CCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHCCC
SMWVMPVAEYLGIKPKFVDSTMIGGSSFVSHMLPAMMALESGQCDAVLVCYGSNQRTSTV
HHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
GRAGISKVRAVLDPQPYEHPYSPLQPITSYALAAARHMHQYGTTREHLAEVAVAARKWAQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
LNPEAMMRDPLSIDDVLNARMVSDPLSVRDCCLVTDGAGAYVMVRADRARDLAKPPVYVL
CCHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCEEEE
GNATATWHRQISCMPDLTVTAAKESGERAFAMAGLKPSDVDVAELYDAFTINTLLFLEDL
ECCCEEEHEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHC
GFCAKGEGGQFVSGGGIAPGGRLPVNTNGGGLSCVHPGMYGMFITIEAVRQLRGECGERQ
CCCCCCCCCCEEECCCCCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCC
VRDAEVAVVHGNGGTLSSQATAILGTGATV
CCCEEEEEEECCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SLSNLRGSVAIVGVGQAGLGEAHGKTEMEILVEAAKAAVRDAGLSMRDIDGIATASVGA
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHCCC
SMWVMPVAEYLGIKPKFVDSTMIGGSSFVSHMLPAMMALESGQCDAVLVCYGSNQRTSTV
HHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHH
GRAGISKVRAVLDPQPYEHPYSPLQPITSYALAAARHMHQYGTTREHLAEVAVAARKWAQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
LNPEAMMRDPLSIDDVLNARMVSDPLSVRDCCLVTDGAGAYVMVRADRARDLAKPPVYVL
CCHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCEEEE
GNATATWHRQISCMPDLTVTAAKESGERAFAMAGLKPSDVDVAELYDAFTINTLLFLEDL
ECCCEEEHEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHC
GFCAKGEGGQFVSGGGIAPGGRLPVNTNGGGLSCVHPGMYGMFITIEAVRQLRGECGERQ
CCCCCCCCCCEEECCCCCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCC
VRDAEVAVVHGNGGTLSSQATAILGTGATV
CCCEEEEEEECCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acyl-CoA; acetyl-CoA [C]

Specific reaction: Acyl-CoA + acetyl-CoA = CoA + oxoacyl-CoA [C]

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]