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The map label for this gene is catB4 [H]

Identifier: 116694487

GI number: 116694487

Start: 606622

End: 607764

Strand: Direct

Name: catB4 [H]

Synonym: H16_B0536

Alternate gene names: 116694487

Gene position: 606622-607764 (Clockwise)

Preceding gene: 116694484

Following gene: 116694488

Centisome position: 20.83

GC content: 70.34

Gene sequence:

>1143_bases
ATGATCAATTCGCATCGACAAGGTATGACCACGACTATCACTTCGGTGGAAGCCATCCTGGTGGACCTGCCCACCATCCG
GGCACACCAGCTGGCCATGGCCACCATGCAGCAGCAGACGCTGGTGATCGTGCGGCTGCGCTGCAGCGACGGCGTGGAGG
GCATCGGCGAGGCCACCACCATCGGCGGCCTGTCCTATGGCGACGAAAGTCCGGAAGGCATCAAGCTGACCATCGACACC
TACCTGGCCCCCGCGCTGGCTGGCCAGGATGCCACCAACGTGCATGGCGCGATGGCGCGCCTGGGCAAGGTCGCGCGCGG
CAACCGCTTCGCCAAGTCAGCGCTGGAGACCGCGTTGCTGGATGCGCAGGGCAAGCGCCTGGGCGTGCCGCTGGCCACGC
TGCTGGGGGGCGCGGTGCGCTCAACCCTGCCGGTGCTGTGGACGCTTGCCAGCGGCGACACCGCCCGCGACATCGCCGAG
GCCGAGCAACTGCTGGCCGAGCGCCGCCACCATACCTTCAAGCTGAAGATCGGCCGGCGCAGCGTGCGCGACGACGTGGC
CCACGTGGCCGCGATCAAGCGCGCGCTGGGCGAGCGGGCGCGCGTCACCGTCGACGTCAACCAGGCCTGGAACGAGGCCG
ACGCCGCCACCGGCATCGCCATGCTGGAAGCCGCCGGCATCGACCTGATCGAGCAGCCCACTCCGCGTGAGCAGCGCATG
GCGCTGGCCCGGCTGGCCGCGCGCTTCGTGGTGCCGATCATGGCCGATGAAGCGGTGTGCGGCCCGGAGGACGCGATGGA
ACTGGCGCGCATCGGCGGCGCCGACGTGTTCGCGCTGAAGATCGCCAAGGCCGGCGGCATCTTCGGCATGCTGCGCACCG
CCGCGGTGGGCGACGCCGCCGGCATCGCGCTGTACGGCGGCACCATGCTGGAGGGCAGCGTCGGCACCATCGCCGCGGCG
CATGGCTTCTGCACGCTGCCGCAGCTGGCCTGGGGCACCGAGCTGTTCGGACCGCTGCTGCTCAAGGACGATATCGTGCA
GCAGCGCCCCGAGTACCGCGACTTCAGCCTGCACCTGCCTGAAGGCCCCGGCCTGGGGCTGGCGCTGGACGAAGACAAGC
TGGCGCACTACCGGCGCGGCTGA

Upstream 100 bases:

>100_bases
CCGGAGTTCCATTGGTGTCCTGCTCCCATACCTGTAGGGTATCGATTGAGACATCATCGGTCTTGGACGCCGGCAAAGTC
AAGCCGCATACTTGGCTTCA

Downstream 100 bases:

>100_bases
ACTGGCAGGCAGCGCACCCCCCGCATGGCACACCGGTGCCTGCACGCATCAAGTGAGGAGACCAATGAAGACGTTATTCC
AGCGGCTGGCAAGCACCGGC

Product: muconate cyclo-isomerase

Products: NA

Alternate protein names: Cis,cis-muconate lactonizing enzyme I; MLE; Muconate cycloisomerase I [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MINSHRQGMTTTITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGVEGIGEATTIGGLSYGDESPEGIKLTIDT
YLAPALAGQDATNVHGAMARLGKVARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRSTLPVLWTLASGDTARDIAE
AEQLLAERRHHTFKLKIGRRSVRDDVAHVAAIKRALGERARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRM
ALARLAARFVVPIMADEAVCGPEDAMELARIGGADVFALKIAKAGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAA
HGFCTLPQLAWGTELFGPLLLKDDIVQQRPEYRDFSLHLPEGPGLGLALDEDKLAHYRRG

Sequences:

>Translated_380_residues
MINSHRQGMTTTITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGVEGIGEATTIGGLSYGDESPEGIKLTIDT
YLAPALAGQDATNVHGAMARLGKVARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRSTLPVLWTLASGDTARDIAE
AEQLLAERRHHTFKLKIGRRSVRDDVAHVAAIKRALGERARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRM
ALARLAARFVVPIMADEAVCGPEDAMELARIGGADVFALKIAKAGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAA
HGFCTLPQLAWGTELFGPLLLKDDIVQQRPEYRDFSLHLPEGPGLGLALDEDKLAHYRRG
>Mature_380_residues
MINSHRQGMTTTITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGVEGIGEATTIGGLSYGDESPEGIKLTIDT
YLAPALAGQDATNVHGAMARLGKVARGNRFAKSALETALLDAQGKRLGVPLATLLGGAVRSTLPVLWTLASGDTARDIAE
AEQLLAERRHHTFKLKIGRRSVRDDVAHVAAIKRALGERARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRM
ALARLAARFVVPIMADEAVCGPEDAMELARIGGADVFALKIAKAGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAA
HGFCTLPQLAWGTELFGPLLLKDDIVQQRPEYRDFSLHLPEGPGLGLALDEDKLAHYRRG

Specific function: Catalyzes a syn cycloisomerization [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=350, Percent_Identity=27.4285714285714, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.1 [H]

Molecular weight: Translated: 40290; Mature: 40290

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSHRQGMTTTITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGVEGIGEATT
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEE
IGGLSYGDESPEGIKLTIDTYLAPALAGQDATNVHGAMARLGKVARGNRFAKSALETALL
ECCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DAQGKRLGVPLATLLGGAVRSTLPVLWTLASGDTARDIAEAEQLLAERRHHTFKLKIGRR
HCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCH
SVRDDVAHVAAIKRALGERARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRM
HHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
ALARLAARFVVPIMADEAVCGPEDAMELARIGGADVFALKIAKAGGIFGMLRTAAVGDAA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
GIALYGGTMLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLLKDDIVQQRPEYRDFSLHLP
CEEEECCEEECCCCCHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECC
EGPGLGLALDEDKLAHYRRG
CCCCCCEEECHHHHHHHCCC
>Mature Secondary Structure
MINSHRQGMTTTITSVEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGVEGIGEATT
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCEE
IGGLSYGDESPEGIKLTIDTYLAPALAGQDATNVHGAMARLGKVARGNRFAKSALETALL
ECCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DAQGKRLGVPLATLLGGAVRSTLPVLWTLASGDTARDIAEAEQLLAERRHHTFKLKIGRR
HCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCH
SVRDDVAHVAAIKRALGERARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRM
HHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCHHHHH
ALARLAARFVVPIMADEAVCGPEDAMELARIGGADVFALKIAKAGGIFGMLRTAAVGDAA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
GIALYGGTMLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLLKDDIVQQRPEYRDFSLHLP
CEEEECCEEECCCCCHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECC
EGPGLGLALDEDKLAHYRRG
CCCCCCEEECHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3609743; 2449420; 2215699; 3612800; 7500361 [H]