BacMap

Definition	Ralstonia eutropha H16 chromosome 2, complete sequence.
Accession	NC_008314
Length	2,912,490

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The map label for this gene is braG [H]

Identifier: 116694453

GI number: 116694453

Start: 566978

End: 567715

Strand: Direct

Name: braG [H]

Synonym: H16_B0502

Alternate gene names: 116694453

Gene position: 566978-567715 (Clockwise)

Preceding gene: 116694452

Following gene: 116694454

Centisome position: 19.47

GC content: 63.55

Gene sequence:

>738_bases
ATGACTTCATTGAACACACCCGCCAACACACCCGCGCTCGAGATCAAGGACCTGCACGCCTGGTACGGCGAATCGCACAT
CCTTCACGGCGTGGATTTGACCGTGAACCGCGGCGAAGTGGTCACGCTGCTGGGCCGCAACGGCGCTGGCCGCACCACCA
CGCTGCGCGCGATCATGGGACTGACCGGCGCGCGCAAGGGCTCGATCAAGGTCAATGGCCACGAGACCATCAGCCTGCCC
ACGCACAAGATCGCGCACTACGGCATTGGCTATTGCCCCGAAGAGCGGGCGATCTTCTCCAGCCTCTCATGCGAAGAGAA
CCTGATGCTGCCGCCGGTGCTGAAGGGCGCCGACACCAGCAAGGGCATGAGCGAAACCGATATCTACGAGATGTTCCCGA
ACCTGAAGGAGCGCCGCCAGAGCCAGGGCACGCGTCTTTCCGGCGGCGAGCAGCAGATGCTGGCGGTCGGGCGCATCCTG
CGCACCGGCGCCAACCTGCTGCTGCTCGATGAAATCTCGGAAGGGTTGGCACCGGTGATCGTGCAGGCGCTTGCGCGCAT
GATCCTGATGCTCAAGCAGAAGGGGTACACGGTGGTGATGGTGGAGCAGAACTTCCGCTTTGCCGCGCCGCTGGCGGACC
GCTTCTACGTGATGGAGCACGGCAGCATCGTCGAGCGCTTCGCGGCCAATGAGCTGGAGGCCAGGATGCCGGTGCTGCAT
GAACTGCTGGGCGTATAA

Upstream 100 bases:

>100_bases
CGTATGCCGAAGTGTCCAGGGATCCGCGCGTGATGGAGGCCTACATGGGCACCGTCGACGCGGAACTGCAGGGCGCGCAC
TGAGAGGGCCGGGGAACGAT

Downstream 100 bases:

>100_bases
GGAGCGACTCATGAATCTGTTTGAAATCCCGCTCCCGGCGCTGCTGTCGCAGTTGTTGCTCGGGCTCGTCAACGGCGCCT
TCTACGCCATGCTGAGCCTG

Product: ABC transporter ATPase

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTSLNTPANTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGARKGSIKVNGHETISLP
THKIAHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADTSKGMSETDIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRIL
RTGANLLLLDEISEGLAPVIVQALARMILMLKQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIVERFAANELEARMPVLH
ELLGV

Sequences:

>Translated_245_residues
MTSLNTPANTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGARKGSIKVNGHETISLP
THKIAHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADTSKGMSETDIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRIL
RTGANLLLLDEISEGLAPVIVQALARMILMLKQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIVERFAANELEARMPVLH
ELLGV
>Mature_244_residues
TSLNTPANTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGARKGSIKVNGHETISLPT
HKIAHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADTSKGMSETDIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILR
TGANLLLLDEISEGLAPVIVQALARMILMLKQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIVERFAANELEARMPVLHE
LLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI47078218, Length=196, Percent_Identity=30.6122448979592, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI45446740, Length=196, Percent_Identity=30.6122448979592, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI31657092, Length=205, Percent_Identity=25.3658536585366, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI116734710, Length=193, Percent_Identity=30.0518134715026, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI21536376, Length=198, Percent_Identity=26.7676767676768, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI150417984, Length=220, Percent_Identity=27.7272727272727, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI27881501, Length=204, Percent_Identity=27.9411764705882, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI30795238, Length=204, Percent_Identity=27.9411764705882, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI105990541, Length=193, Percent_Identity=25.9067357512953, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI9961252, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4505771, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI9961250, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI27262624, Length=200, Percent_Identity=27, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27262626, Length=200, Percent_Identity=27, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI153792144, Length=211, Percent_Identity=26.5402843601896, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI42741659, Length=244, Percent_Identity=25.4098360655738, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI9955963, Length=221, Percent_Identity=26.6968325791855, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI27477115, Length=218, Percent_Identity=28.4403669724771, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27436953, Length=196, Percent_Identity=27.0408163265306, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI148612844, Length=234, Percent_Identity=26.0683760683761, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI255708477, Length=234, Percent_Identity=26.0683760683761, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87082268, Length=232, Percent_Identity=40.948275862069, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1789593, Length=216, Percent_Identity=34.7222222222222, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789672, Length=230, Percent_Identity=29.1304347826087, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788225, Length=232, Percent_Identity=33.6206896551724, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787029, Length=218, Percent_Identity=29.3577981651376, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788897, Length=213, Percent_Identity=31.4553990610329, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787792, Length=226, Percent_Identity=29.646017699115, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787105, Length=222, Percent_Identity=26.5765765765766, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=28.7671232876712, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI87081791, Length=221, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788761, Length=229, Percent_Identity=27.9475982532751, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788644, Length=235, Percent_Identity=30.2127659574468, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787089, Length=221, Percent_Identity=26.2443438914027, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790525, Length=226, Percent_Identity=28.7610619469027, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786253, Length=237, Percent_Identity=30.379746835443, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI48995001, Length=225, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=23.9043824701195, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787370, Length=224, Percent_Identity=27.2321428571429, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787112, Length=242, Percent_Identity=30.5785123966942, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790190, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786703, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789991, Length=238, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787712, Length=229, Percent_Identity=25.764192139738, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786319, Length=228, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786398, Length=210, Percent_Identity=25.2380952380952, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081782, Length=234, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790467, Length=215, Percent_Identity=26.046511627907, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081709, Length=222, Percent_Identity=27.4774774774775, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786872, Length=216, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1788210, Length=235, Percent_Identity=28.5106382978723, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788165, Length=217, Percent_Identity=27.1889400921659, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994943, Length=231, Percent_Identity=22.9437229437229, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789873, Length=206, Percent_Identity=23.3009708737864, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786698, Length=168, Percent_Identity=25.5952380952381, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082267, Length=215, Percent_Identity=26.5116279069767, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=199, Percent_Identity=29.6482412060301, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=218, Percent_Identity=28.8990825688073, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=202, Percent_Identity=28.7128712871287, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983510, Length=202, Percent_Identity=28.7128712871287, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983523, Length=202, Percent_Identity=28.7128712871287, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17565586, Length=218, Percent_Identity=27.5229357798165, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25146777, Length=204, Percent_Identity=24.5098039215686, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI193211017, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71996809, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193211015, Length=225, Percent_Identity=25.7777777777778, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193202349, Length=241, Percent_Identity=25.3112033195021, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996550, Length=232, Percent_Identity=25.4310344827586, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=208, Percent_Identity=27.4038461538462, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71984940, Length=233, Percent_Identity=26.6094420600858, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=231, Percent_Identity=25.974025974026, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17569145, Length=226, Percent_Identity=25.2212389380531, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24648314, Length=212, Percent_Identity=27.8301886792453, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24666092, Length=211, Percent_Identity=28.9099526066351, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI221512771, Length=213, Percent_Identity=27.6995305164319, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28574150, Length=222, Percent_Identity=28.3783783783784, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI116007328, Length=222, Percent_Identity=28.3783783783784, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24643674, Length=215, Percent_Identity=27.906976744186, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573573, Length=230, Percent_Identity=25.2173913043478, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28573571, Length=230, Percent_Identity=25.2173913043478, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24650855, Length=232, Percent_Identity=26.2931034482759, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650853, Length=232, Percent_Identity=26.2931034482759, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281362751, Length=234, Percent_Identity=25.6410256410256, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI85725262, Length=203, Percent_Identity=28.0788177339901, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357907, Length=223, Percent_Identity=28.6995515695067, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550390, Length=233, Percent_Identity=24.0343347639485, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363262, Length=226, Percent_Identity=24.3362831858407, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24653245, Length=226, Percent_Identity=24.3362831858407, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26910; Mature: 26778

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLNTPANTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMG
CCCCCCCCCCCCEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHH
LTGARKGSIKVNGHETISLPTHKIAHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADTS
CCCCCCCCEEECCCEEEECCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
KGMSETDIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPVI
CCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHH
VQALARMILMLKQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIVERFAANELEARMPVLH
HHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
ELLGV
HHHCC
>Mature Secondary Structure 
TSLNTPANTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMG
CCCCCCCCCCCEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHH
LTGARKGSIKVNGHETISLPTHKIAHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADTS
CCCCCCCCEEECCCEEEECCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
KGMSETDIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPVI
CCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHH
VQALARMILMLKQKGYTVVMVEQNFRFAAPLADRFYVMEHGSIVERFAANELEARMPVLH
HHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
ELLGV
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]