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The map label for this gene is paaF [C]

Identifier: 116694372

GI number: 116694372

Start: 479588

End: 480364

Strand: Direct

Name: paaF [C]

Synonym: H16_B0420

Alternate gene names: 116694372

Gene position: 479588-480364 (Clockwise)

Preceding gene: 116694371

Following gene: 116694373

Centisome position: 16.47

GC content: 67.44

Gene sequence:

>777_bases
ATGAACGACTGGACCCAGTTGAAGTTGTCCGTGGATCGCCACATCGCGGTCGTTACGATGGATGCGCCGCCCGTCAATGC
GCTGACGCGCGGGCTCAACGACGAGCTCGTTGCCGTGCTCGACCAGCTTTCCGACACACCCGAGGTACGCGTCATTGTCC
TGACAGGCGCAGGCAAGGTGTTCTGCGCCGGCGCGGACATCAAGGGCCGTGGCGCGCTGGTCGATGGCCCCGGCGCCCTG
CGCGCCCATTCGCGCCGCACACGGGAGTGCTTCCATGCGATCCGTGAATGTGCCAAGCCCGTGGTGGCGGCGCTCAATGG
CCCCGCGCTGGGCGCGGGACTGGCCATTGCCGCTTCCGCAGACATCCTCGTGGCCAGCGAGCAGGCCAGCGCGGGTTTGC
CGGAAATCGACGTCGGGGCGCTTGGCGGCGGCCGGCATGCCATGCGCCTGTTCGGGCATTCGCGCGTGCGCCGCATGATG
CTGACCGGCTATCGCGTTCCCGCGCCCGAGCTGTATCGCCTCGGCATCGTCGAAGCGTGCGTGGCGCCCGATGCACTGAT
GCCGGCAGCGCTGGAGCTGGCGGAACAGGTCGCCTCCAAGAGTCCGGTGGTGTCCCAGCTTGCCAAGCACACCCTCAATA
CGATCGAGCACATGGGCTTGCGCGACGGCTATCGCTACGAACAGGACCAGACCGCCATCCTCTCGAAGACGGAAGACTGC
AGGGAGGCACAGGCGGCATTCCGCGAGAAGCGGGCGCCGGTCTTCGTCGGCCGCTGA

Upstream 100 bases:

>100_bases
ACGGCAGTCAGGCGATTCCTGGACAAGGAGCCGCCGCTGTTCTCGTGGCCGCGCCGGCGCGACTGAACCTCGACCAGCCA
ATCCACAACCAGGAGGAAAC

Downstream 100 bases:

>100_bases
GGGTGCTTCGGGCACGGCCGTGTAACAACTTCCCGCCTGCGTGTTGCCGATTCTGTCAAATGCGGGCAGGTGCCCGCCCA
TATAGTGATGACGCACAGGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNDWTQLKLSVDRHIAVVTMDAPPVNALTRGLNDELVAVLDQLSDTPEVRVIVLTGAGKVFCAGADIKGRGALVDGPGAL
RAHSRRTRECFHAIRECAKPVVAALNGPALGAGLAIAASADILVASEQASAGLPEIDVGALGGGRHAMRLFGHSRVRRMM
LTGYRVPAPELYRLGIVEACVAPDALMPAALELAEQVASKSPVVSQLAKHTLNTIEHMGLRDGYRYEQDQTAILSKTEDC
REAQAAFREKRAPVFVGR

Sequences:

>Translated_258_residues
MNDWTQLKLSVDRHIAVVTMDAPPVNALTRGLNDELVAVLDQLSDTPEVRVIVLTGAGKVFCAGADIKGRGALVDGPGAL
RAHSRRTRECFHAIRECAKPVVAALNGPALGAGLAIAASADILVASEQASAGLPEIDVGALGGGRHAMRLFGHSRVRRMM
LTGYRVPAPELYRLGIVEACVAPDALMPAALELAEQVASKSPVVSQLAKHTLNTIEHMGLRDGYRYEQDQTAILSKTEDC
REAQAAFREKRAPVFVGR
>Mature_258_residues
MNDWTQLKLSVDRHIAVVTMDAPPVNALTRGLNDELVAVLDQLSDTPEVRVIVLTGAGKVFCAGADIKGRGALVDGPGAL
RAHSRRTRECFHAIRECAKPVVAALNGPALGAGLAIAASADILVASEQASAGLPEIDVGALGGGRHAMRLFGHSRVRRMM
LTGYRVPAPELYRLGIVEACVAPDALMPAALELAEQVASKSPVVSQLAKHTLNTIEHMGLRDGYRYEQDQTAILSKTEDC
REAQAAFREKRAPVFVGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=33.8709677419355, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=27.906976744186, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI68989263, Length=207, Percent_Identity=29.4685990338164, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI37594469, Length=213, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI37594471, Length=213, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI20127408, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=29.8449612403101, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI31542718, Length=235, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=33.2046332046332, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=34.2105263157895, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI87082183, Length=247, Percent_Identity=26.7206477732794, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=27.8225806451613, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=27.9527559055118, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=32.3529411764706, Blast_Score=78, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=33.7301587301587, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=33.8709677419355, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=29.2792792792793, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=259, Percent_Identity=27.7992277992278, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17534483, Length=268, Percent_Identity=27.6119402985075, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=29.045643153527, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=189, Percent_Identity=33.8624338624339, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=189, Percent_Identity=33.8624338624339, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=189, Percent_Identity=33.8624338624339, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=177, Percent_Identity=31.0734463276836, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25.390625, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560910, Length=234, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=262, Percent_Identity=33.969465648855, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24653477, Length=262, Percent_Identity=33.969465648855, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=30.5555555555556, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19920382, Length=247, Percent_Identity=27.5303643724696, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922422, Length=239, Percent_Identity=25.1046025104602, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27518; Mature: 27518

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDWTQLKLSVDRHIAVVTMDAPPVNALTRGLNDELVAVLDQLSDTPEVRVIVLTGAGKV
CCCHHEEEEEECCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEE
FCAGADIKGRGALVDGPGALRAHSRRTRECFHAIRECAKPVVAALNGPALGAGLAIAASA
EEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
DILVASEQASAGLPEIDVGALGGGRHAMRLFGHSRVRRMMLTGYRVPAPELYRLGIVEAC
CEEEECCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAPDALMPAALELAEQVASKSPVVSQLAKHTLNTIEHMGLRDGYRYEQDQTAILSKTEDC
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHH
REAQAAFREKRAPVFVGR
HHHHHHHHHHCCCEECCC
>Mature Secondary Structure
MNDWTQLKLSVDRHIAVVTMDAPPVNALTRGLNDELVAVLDQLSDTPEVRVIVLTGAGKV
CCCHHEEEEEECCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCEE
FCAGADIKGRGALVDGPGALRAHSRRTRECFHAIRECAKPVVAALNGPALGAGLAIAASA
EEECCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
DILVASEQASAGLPEIDVGALGGGRHAMRLFGHSRVRRMMLTGYRVPAPELYRLGIVEAC
CEEEECCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VAPDALMPAALELAEQVASKSPVVSQLAKHTLNTIEHMGLRDGYRYEQDQTAILSKTEDC
HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHH
REAQAAFREKRAPVFVGR
HHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA