BacMap

Definition	Ralstonia eutropha H16 chromosome 2, complete sequence.
Accession	NC_008314
Length	2,912,490

Click here to switch to the map view.

The map label for this gene is crt [H]

Identifier: 116694371

GI number: 116694371

Start: 478750

End: 479553

Strand: Direct

Name: crt [H]

Synonym: H16_B0419

Alternate gene names: 116694371

Gene position: 478750-479553 (Clockwise)

Preceding gene: 116694370

Following gene: 116694372

Centisome position: 16.44

GC content: 66.67

Gene sequence:

>804_bases
ATGTACGAAGCTATCCAGTACGAGAACGAAGATGGCGTGGGCGTGCTCACGCTGGAGCGCCCGCAAACCAAGAATGCCGT
GGACGCCGTCATGCGCCGCGAACTGCCACAGTTGATGGGCGCCCTGAACACCGACCGCACGCTCAAGGTACTCATCGTCA
CTGGCGCCGGCGGCAATTTCTGTTCGGGTGGCGACCTGCGCGCGGTGCGCGACAGCGCGGGCAGCGAAGGCTCGGCGGAG
GGGCGCCGCCAGCGCATCCTGGACGGGCAGGCTACCCCCGGCGCGTTCCTGAATTTCGACCGCCCTGTCATCGCGGTGGT
GGAGGGCGTTGCCTATGGGGCTGGCTGCTCGCTGGCACTGACCGCTGACATGGTCCTCGCCGCGCGCGACGCGCGCTTTT
GCCTGTCGTTCGGCAGGATCGGCGCGGTGCCGGACTACGGCGCCTTCTATACGCTGCCGCGCATCGTCGGATTGCAACGG
GCGAAGGAACTCGTTTTCTCCGCGCGCGAATTCGGGGCGGCCGAGGCACGCGATCTCGGCATCGTGATGGAGGTGCACGA
GCCCGGTACCGTGCTCGACCGGGCGCGGGAGATTGCCCGCAGCATGGCGCAAGCGTCGCCCCTCGCGCTCAGCGTATCCA
AGCGCGCGTTGAATACCTCTCTCCATTCCGATCTGCCGACCATGCTGATGCACGAAGCAGACGGGCAGGGTATTGCGATG
AGCACCGACTATCACCTGACGGCAGTCAGGCGATTCCTGGACAAGGAGCCGCCGCTGTTCTCGTGGCCGCGCCGGCGCGA
CTGA

Upstream 100 bases:

>100_bases
AGTTCGGCAGCTTCATCGTCTCCGAGCGGGTCAAATGGAAGGCGGTGATCGAACGCACCGGCGCACGGATCGAATAACGC
ATCACCACGAGGAGATCACC

Downstream 100 bases:

>100_bases
ACCTCGACCAGCCAATCCACAACCAGGAGGAAACATGAACGACTGGACCCAGTTGAAGTTGTCCGTGGATCGCCACATCG
CGGTCGTTACGATGGATGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MYEAIQYENEDGVGVLTLERPQTKNAVDAVMRRELPQLMGALNTDRTLKVLIVTGAGGNFCSGGDLRAVRDSAGSEGSAE
GRRQRILDGQATPGAFLNFDRPVIAVVEGVAYGAGCSLALTADMVLAARDARFCLSFGRIGAVPDYGAFYTLPRIVGLQR
AKELVFSAREFGAAEARDLGIVMEVHEPGTVLDRAREIARSMAQASPLALSVSKRALNTSLHSDLPTMLMHEADGQGIAM
STDYHLTAVRRFLDKEPPLFSWPRRRD

Sequences:

>Translated_267_residues
MYEAIQYENEDGVGVLTLERPQTKNAVDAVMRRELPQLMGALNTDRTLKVLIVTGAGGNFCSGGDLRAVRDSAGSEGSAE
GRRQRILDGQATPGAFLNFDRPVIAVVEGVAYGAGCSLALTADMVLAARDARFCLSFGRIGAVPDYGAFYTLPRIVGLQR
AKELVFSAREFGAAEARDLGIVMEVHEPGTVLDRAREIARSMAQASPLALSVSKRALNTSLHSDLPTMLMHEADGQGIAM
STDYHLTAVRRFLDKEPPLFSWPRRRD
>Mature_267_residues
MYEAIQYENEDGVGVLTLERPQTKNAVDAVMRRELPQLMGALNTDRTLKVLIVTGAGGNFCSGGDLRAVRDSAGSEGSAE
GRRQRILDGQATPGAFLNFDRPVIAVVEGVAYGAGCSLALTADMVLAARDARFCLSFGRIGAVPDYGAFYTLPRIVGLQR
AKELVFSAREFGAAEARDLGIVMEVHEPGTVLDRAREIARSMAQASPLALSVSKRALNTSLHSDLPTMLMHEADGQGIAM
STDYHLTAVRRFLDKEPPLFSWPRRRD

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=259, Percent_Identity=28.1853281853282, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI45643119, Length=259, Percent_Identity=28.1853281853282, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI260275230, Length=259, Percent_Identity=28.1853281853282, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=30, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI194097323, Length=215, Percent_Identity=25.5813953488372, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI221136756, Length=265, Percent_Identity=25.6603773584906, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI221136753, Length=265, Percent_Identity=25.6603773584906, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI157694516, Length=252, Percent_Identity=26.984126984127, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI213417737, Length=252, Percent_Identity=26.984126984127, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221307494, Length=265, Percent_Identity=25.6603773584906, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI70995211, Length=210, Percent_Identity=30.4761904761905, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI116235487, Length=213, Percent_Identity=28.6384976525822, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4757966, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI51538581, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI25453481, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI51538573, Length=233, Percent_Identity=26.1802575107296, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI37594471, Length=161, Percent_Identity=29.8136645962733, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4757968, Length=233, Percent_Identity=25.7510729613734, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI49355787, Length=233, Percent_Identity=25.7510729613734, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI37594469, Length=161, Percent_Identity=29.8136645962733, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI31542718, Length=259, Percent_Identity=26.2548262548263, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=222, Percent_Identity=28.3783783783784, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI221142681, Length=234, Percent_Identity=35.4700854700855, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=35.9848484848485, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=33.0645161290323, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788597, Length=220, Percent_Identity=33.6363636363636, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082183, Length=217, Percent_Identity=23.0414746543779, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=26.0416666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=248, Percent_Identity=24.5967741935484, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=30.5309734513274, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17535521, Length=233, Percent_Identity=27.8969957081545, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=29.7297297297297, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=250, Percent_Identity=26.4, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=28.0542986425339, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=27.6264591439689, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=240, Percent_Identity=27.0833333333333, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=215, Percent_Identity=28.3720930232558, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=23.7837837837838, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=192, Percent_Identity=25, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=231, Percent_Identity=24.6753246753247, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=231, Percent_Identity=24.6753246753247, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=28.125, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=28.125, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24653139, Length=255, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21357171, Length=234, Percent_Identity=29.9145299145299, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19920382, Length=215, Percent_Identity=29.7674418604651, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24654903, Length=186, Percent_Identity=25.8064516129032, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28571730, Length=145, Percent_Identity=28.9655172413793, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571729, Length=145, Percent_Identity=28.9655172413793, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24650670, Length=265, Percent_Identity=26.4150943396226, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28926; Mature: 28926

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEAIQYENEDGVGVLTLERPQTKNAVDAVMRRELPQLMGALNTDRTLKVLIVTGAGGNF
CCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
CSGGDLRAVRDSAGSEGSAEGRRQRILDGQATPGAFLNFDRPVIAVVEGVAYGAGCSLAL
CCCCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEH
TADMVLAARDARFCLSFGRIGAVPDYGAFYTLPRIVGLQRAKELVFSAREFGAAEARDLG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IVMEVHEPGTVLDRAREIARSMAQASPLALSVSKRALNTSLHSDLPTMLMHEADGQGIAM
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCHHHHHCCCCCCEEEE
STDYHLTAVRRFLDKEPPLFSWPRRRD
ECCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MYEAIQYENEDGVGVLTLERPQTKNAVDAVMRRELPQLMGALNTDRTLKVLIVTGAGGNF
CCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
CSGGDLRAVRDSAGSEGSAEGRRQRILDGQATPGAFLNFDRPVIAVVEGVAYGAGCSLAL
CCCCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEH
TADMVLAARDARFCLSFGRIGAVPDYGAFYTLPRIVGLQRAKELVFSAREFGAAEARDLG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IVMEVHEPGTVLDRAREIARSMAQASPLALSVSKRALNTSLHSDLPTMLMHEADGQGIAM
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCHHHHHCCCCCCEEEE
STDYHLTAVRRFLDKEPPLFSWPRRRD
ECCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]