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The map label for this gene is sbcD [H]

Identifier: 116694150

GI number: 116694150

Start: 219506

End: 220693

Strand: Direct

Name: sbcD [H]

Synonym: H16_B0195

Alternate gene names: 116694150

Gene position: 219506-220693 (Clockwise)

Preceding gene: 116694148

Following gene: 116694151

Centisome position: 7.54

GC content: 70.03

Gene sequence:

>1188_bases
ATGATGCGGGTTTCGCCTTTGCATCTATCGGATCCCGGATTGCGTTTCCTTCACACCGCCGACTGGCATCTCGGCCGGCT
CTTCCATGCGCGCAGCCTGCTGGAAGACCAGGCCCATATCCTCGACCAGTTTGTCGAACTGGTCCGCACCGAACGCCCCG
ATGCCGTGCTGATCGCCGGCGACGTCTACGACCGCGCCGTGCCGCCGCCTGAAGCAGTGGCCTTGCTCGACGACGTGCTG
GGCCGCATCGTCATCGACACCGGAGTGCCGGTGGTGATGATCGCCGGCAACCATGACAGCGCGCAGCGGCTGGGATTCGG
CGCGCGGCTGATGCGGGCGCAGGGCCTGCACGTGGCCGGCCGCACGCTGGCCGCGGCGGCCTGCGTGACGCTGCACGACA
CCCACGGCGAGGTGCGCATCTACGCGCTGCCGTACGCGGAACCGGCGGTGGTGCGCGATGCCATGGGCACCGACCTGCCA
TCGCATGAAGCCGCGCTGCGCGCGCAGCTCGATGCCATCCGCGCGGTGCATCCGCCGGGCGTGCGTGCCGTGGTGGTGGG
CCATGCCTTCGTGGTGGGCGGCGCGGCAAGCGAATCGGAACGGCCGCTGTCGGTGGGCGGCAGCGGCGCGGTGGCCGCGG
ACCTGTTCGCCGGCTTTGACCTGGTGGCGCTGGGCCACCTGCACCGGCCCCAGACGCTGGGCGGCGGGCGCGTTCACTAT
GCGGGCTCGCTGCTGAAGTATTCGCTGTCAGAGTGCGCGCACGACAAGTCGGTGTCGCGCATCGAACTGGGCGCGGACGG
CGCGGTCGCCATCACGCCGGTGCCGCTGCAGGCGCTGCGCGATGTGCGCGTGGTCGAAGGCGAACTGGCCGCGGTGCTGG
CGGCGGGTGTCGATGATCCGCAACGCGATGACTACATCCACGCGCGGCTCACCGATACGGGCGCGCTGCTGGACCCGATG
GCCAGGCTGCGGCAGGTCTATCCCAATGCGCTCGCGATCGAACGCACGGTGCTGGCGCGCACCGGCACGGCTTCGGAGGC
CGGGCGCAAGTTGCGGCAACTGGGTACCGGCGAACTCTTTGCCAACTTCTTCCGCGAAGTGGCGGATGCCGAGCTGGAAC
CGGACCAGCGTGCCGTGCTCGACCAGGTGCTGACCGGGATCGCCGCCGCGGACAGGGAGTCGGCATGA

Upstream 100 bases:

>100_bases
ATGTGCGTTCTCCGAAAATTTCAAATTTGGGGCGACTGCATGCATGATGCGCGCGCCCGCCTGAACCGTTGCTGAACGGG
CAGTCAGGTCGCAACGCATT

Downstream 100 bases:

>100_bases
GACCGCTGCATTTGACGCTGCAGGCCTTCGGTCCCTTTGCCGCGACCGAGCAGGTCGATTTCACGCGGCTGGGCGAGCAG
GCCTTCGTACTGATCCATGG

Product: DNA repair exonuclease, SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MMRVSPLHLSDPGLRFLHTADWHLGRLFHARSLLEDQAHILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAVALLDDVL
GRIVIDTGVPVVMIAGNHDSAQRLGFGARLMRAQGLHVAGRTLAAAACVTLHDTHGEVRIYALPYAEPAVVRDAMGTDLP
SHEAALRAQLDAIRAVHPPGVRAVVVGHAFVVGGAASESERPLSVGGSGAVAADLFAGFDLVALGHLHRPQTLGGGRVHY
AGSLLKYSLSECAHDKSVSRIELGADGAVAITPVPLQALRDVRVVEGELAAVLAAGVDDPQRDDYIHARLTDTGALLDPM
ARLRQVYPNALAIERTVLARTGTASEAGRKLRQLGTGELFANFFREVADAELEPDQRAVLDQVLTGIAAADRESA

Sequences:

>Translated_395_residues
MMRVSPLHLSDPGLRFLHTADWHLGRLFHARSLLEDQAHILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAVALLDDVL
GRIVIDTGVPVVMIAGNHDSAQRLGFGARLMRAQGLHVAGRTLAAAACVTLHDTHGEVRIYALPYAEPAVVRDAMGTDLP
SHEAALRAQLDAIRAVHPPGVRAVVVGHAFVVGGAASESERPLSVGGSGAVAADLFAGFDLVALGHLHRPQTLGGGRVHY
AGSLLKYSLSECAHDKSVSRIELGADGAVAITPVPLQALRDVRVVEGELAAVLAAGVDDPQRDDYIHARLTDTGALLDPM
ARLRQVYPNALAIERTVLARTGTASEAGRKLRQLGTGELFANFFREVADAELEPDQRAVLDQVLTGIAAADRESA
>Mature_395_residues
MMRVSPLHLSDPGLRFLHTADWHLGRLFHARSLLEDQAHILDQFVELVRTERPDAVLIAGDVYDRAVPPPEAVALLDDVL
GRIVIDTGVPVVMIAGNHDSAQRLGFGARLMRAQGLHVAGRTLAAAACVTLHDTHGEVRIYALPYAEPAVVRDAMGTDLP
SHEAALRAQLDAIRAVHPPGVRAVVVGHAFVVGGAASESERPLSVGGSGAVAADLFAGFDLVALGHLHRPQTLGGGRVHY
AGSLLKYSLSECAHDKSVSRIELGADGAVAITPVPLQALRDVRVVEGELAAVLAAGVDDPQRDDYIHARLTDTGALLDPM
ARLRQVYPNALAIERTVLARTGTASEAGRKLRQLGTGELFANFFREVADAELEPDQRAVLDQVLTGIAAADRESA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=396, Percent_Identity=26.7676767676768, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 42006; Mature: 42006

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRVSPLHLSDPGLRFLHTADWHLGRLFHARSLLEDQAHILDQFVELVRTERPDAVLIAG
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
DVYDRAVPPPEAVALLDDVLGRIVIDTGVPVVMIAGNHDSAQRLGFGARLMRAQGLHVAG
CHHHCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHH
RTLAAAACVTLHDTHGEVRIYALPYAEPAVVRDAMGTDLPSHEAALRAQLDAIRAVHPPG
HHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
VRAVVVGHAFVVGGAASESERPLSVGGSGAVAADLFAGFDLVALGHLHRPQTLGGGRVHY
CEEEEEECEEEECCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCEEEH
AGSLLKYSLSECAHDKSVSRIELGADGAVAITPVPLQALRDVRVVEGELAAVLAAGVDDP
HHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
QRDDYIHARLTDTGALLDPMARLRQVYPNALAIERTVLARTGTASEAGRKLRQLGTGELF
CCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
ANFFREVADAELEPDQRAVLDQVLTGIAAADRESA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMRVSPLHLSDPGLRFLHTADWHLGRLFHARSLLEDQAHILDQFVELVRTERPDAVLIAG
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
DVYDRAVPPPEAVALLDDVLGRIVIDTGVPVVMIAGNHDSAQRLGFGARLMRAQGLHVAG
CHHHCCCCCHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHH
RTLAAAACVTLHDTHGEVRIYALPYAEPAVVRDAMGTDLPSHEAALRAQLDAIRAVHPPG
HHHHHHHEEEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
VRAVVVGHAFVVGGAASESERPLSVGGSGAVAADLFAGFDLVALGHLHRPQTLGGGRVHY
CEEEEEECEEEECCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCEEEH
AGSLLKYSLSECAHDKSVSRIELGADGAVAITPVPLQALRDVRVVEGELAAVLAAGVDDP
HHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCC
QRDDYIHARLTDTGALLDPMARLRQVYPNALAIERTVLARTGTASEAGRKLRQLGTGELF
CCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHH
ANFFREVADAELEPDQRAVLDQVLTGIAAADRESA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA