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The map label for this gene is cpaF2 [C]

Identifier: 116694142

GI number: 116694142

Start: 211419

End: 212735

Strand: Direct

Name: cpaF2 [C]

Synonym: H16_B0187

Alternate gene names: 116694142

Gene position: 211419-212735 (Clockwise)

Preceding gene: 116694141

Following gene: 116694143

Centisome position: 7.26

GC content: 66.97

Gene sequence:

>1317_bases
ATGACGCAAGCGATTGAATTCTCCGACAACGCCCCCGCACCGTTTCCGGTCTCGCAGGAATTCCAGAACATCAAGGAAGC
GGCGCACGAGCACCTGCTGACCCGCATCGAAGAGCTCGGCGCAGAGTTCGGCCGCTGGTCGCGTACCGCGATCCAGCGCT
TTGTCGACCTGGAACTGGAGAGCTTTACCCGGTTGCGCCGCATCCCCATCAACGAGGCCGAGCTGCGCCAGATTGCCGAG
GCGCTGACCAAGGAGCTGGCCGGCTTCGGCCCGATCGAAGACCTGCTCAACGACCCCGCGGTGGAAGACATCCTGGTCAA
CGGCCACCTTGACGTCTACGTGTCGCGCCACGGCGTGCTGGAGCGCATCCCGGTGCGCTTTACCGACAGCGGCCACCTGC
TGCGCATCGTGCGCCGCATCCTGGCGCCGATCGGCAGGCGGCTGGACGAGTCCAACCCGATGGTCGATGCGCGCCTGCCC
GACGGCGGCCGCATCAACGTGGTGATCCCGCCGCTGGCGCTGGAGGGTCCAGTGGTGTCGATCCGCAAGTTCCGCAAGGA
CCCGATGACGCCTGCTGACCTGCAGGGGCTGGGCACCATGAGCCCCGAGATCTGCGACCTGCTGCAGGCCGCGGTGCAGG
CGCGCTGCAATATCCTGGTGAGCGGCGGCACCAGCTCGGGCAAGACTTCGCTGCTCAATGCGCTGGCCACCTTCGTGCCG
ACCACCGAGCGCGTCATCACCATCGAGGACACCGCCGAGCTGGCGCTGAACCACCCGCACGTGGTGCGGCTGGAAAGCCG
TCCCGGCGGTTTCGAGGGCACCGGCGTGGTGTCGATCCGCGACCTGCTGCGCAACAGCCTGCGCATGCGCCCGGACCGCA
TCATCGTGGGTGAAGTGCGCGGCGGCGAGGTGCTGGAGATGCTCCAGGCGATGAGCACCGGCCACGATGGTTCGATGGGC
ACCATCCACGCCAGCAGCCCGCGCGAATGCCTGTACCGGCTGGAGATGCTGGCCGGCTTTGCCGGCTTCCAGGGCAGCGA
GGCGAGCCTGCGCCGGCAGATCGCCAACGCCATCGACTTTATCGTGCAGATCGCGCGGCTTTCCAACGGCAAGCGCCGCA
TCGTCTCGATCACCGAGATCACCGGGCTGGGCGACAACATCATTTCCACCCAGGAGCTGTACCGTCACGAGCCCTACGTG
AACCCGGACGGTACCGAGACCGACCGCTGGCTCTCGCTCGGCCTGCTGCCGCACTCGCCCAAGCTGGCACGCATGCGCGG
ACCGGGCTTCATGCTGGACGACCGCTTCGATGTCTAG

Upstream 100 bases:

>100_bases
CGCGCGGCGCAGGCCAGGGAAACCAGCCTGCTGGCGCGGATGAAGGACAAACTGCCTGAGGCGCTGCGCGCGGTGCGCGT
GGCACGCACTGGAACCTGAC

Downstream 100 bases:

>100_bases
CGCCACGCTGCTGCTCGCCGCCACGGCCCTGGTCATCACCGGCCTGGGCCTGCTGCTGCTGGCCAGCGCGCAGGCGCGTG
CGCGCCGCGAGCAGCAGCAG

Product: flp pilus assembly ATPase CpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTQAIEFSDNAPAPFPVSQEFQNIKEAAHEHLLTRIEELGAEFGRWSRTAIQRFVDLELESFTRLRRIPINEAELRQIAE
ALTKELAGFGPIEDLLNDPAVEDILVNGHLDVYVSRHGVLERIPVRFTDSGHLLRIVRRILAPIGRRLDESNPMVDARLP
DGGRINVVIPPLALEGPVVSIRKFRKDPMTPADLQGLGTMSPEICDLLQAAVQARCNILVSGGTSSGKTSLLNALATFVP
TTERVITIEDTAELALNHPHVVRLESRPGGFEGTGVVSIRDLLRNSLRMRPDRIIVGEVRGGEVLEMLQAMSTGHDGSMG
TIHASSPRECLYRLEMLAGFAGFQGSEASLRRQIANAIDFIVQIARLSNGKRRIVSITEITGLGDNIISTQELYRHEPYV
NPDGTETDRWLSLGLLPHSPKLARMRGPGFMLDDRFDV

Sequences:

>Translated_438_residues
MTQAIEFSDNAPAPFPVSQEFQNIKEAAHEHLLTRIEELGAEFGRWSRTAIQRFVDLELESFTRLRRIPINEAELRQIAE
ALTKELAGFGPIEDLLNDPAVEDILVNGHLDVYVSRHGVLERIPVRFTDSGHLLRIVRRILAPIGRRLDESNPMVDARLP
DGGRINVVIPPLALEGPVVSIRKFRKDPMTPADLQGLGTMSPEICDLLQAAVQARCNILVSGGTSSGKTSLLNALATFVP
TTERVITIEDTAELALNHPHVVRLESRPGGFEGTGVVSIRDLLRNSLRMRPDRIIVGEVRGGEVLEMLQAMSTGHDGSMG
TIHASSPRECLYRLEMLAGFAGFQGSEASLRRQIANAIDFIVQIARLSNGKRRIVSITEITGLGDNIISTQELYRHEPYV
NPDGTETDRWLSLGLLPHSPKLARMRGPGFMLDDRFDV
>Mature_437_residues
TQAIEFSDNAPAPFPVSQEFQNIKEAAHEHLLTRIEELGAEFGRWSRTAIQRFVDLELESFTRLRRIPINEAELRQIAEA
LTKELAGFGPIEDLLNDPAVEDILVNGHLDVYVSRHGVLERIPVRFTDSGHLLRIVRRILAPIGRRLDESNPMVDARLPD
GGRINVVIPPLALEGPVVSIRKFRKDPMTPADLQGLGTMSPEICDLLQAAVQARCNILVSGGTSSGKTSLLNALATFVPT
TERVITIEDTAELALNHPHVVRLESRPGGFEGTGVVSIRDLLRNSLRMRPDRIIVGEVRGGEVLEMLQAMSTGHDGSMGT
IHASSPRECLYRLEMLAGFAGFQGSEASLRRQIANAIDFIVQIARLSNGKRRIVSITEITGLGDNIISTQELYRHEPYVN
PDGTETDRWLSLGLLPHSPKLARMRGPGFMLDDRFDV

Specific function: Unknown

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=247, Percent_Identity=28.3400809716599, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 48443; Mature: 48312

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAIEFSDNAPAPFPVSQEFQNIKEAAHEHLLTRIEELGAEFGRWSRTAIQRFVDLELE
CCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFTRLRRIPINEAELRQIAEALTKELAGFGPIEDLLNDPAVEDILVNGHLDVYVSRHGVL
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHEEECCCEEEEEECCCCH
ERIPVRFTDSGHLLRIVRRILAPIGRRLDESNPMVDARLPDGGRINVVIPPLALEGPVVS
HHCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHH
IRKFRKDPMTPADLQGLGTMSPEICDLLQAAVQARCNILVSGGTSSGKTSLLNALATFVP
HHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCC
TTERVITIEDTAELALNHPHVVRLESRPGGFEGTGVVSIRDLLRNSLRMRPDRIIVGEVR
CCCEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
GGEVLEMLQAMSTGHDGSMGTIHASSPRECLYRLEMLAGFAGFQGSEASLRRQIANAIDF
CCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IVQIARLSNGKRRIVSITEITGLGDNIISTQELYRHEPYVNPDGTETDRWLSLGLLPHSP
HHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
KLARMRGPGFMLDDRFDV
CHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQAIEFSDNAPAPFPVSQEFQNIKEAAHEHLLTRIEELGAEFGRWSRTAIQRFVDLELE
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFTRLRRIPINEAELRQIAEALTKELAGFGPIEDLLNDPAVEDILVNGHLDVYVSRHGVL
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHEEECCCEEEEEECCCCH
ERIPVRFTDSGHLLRIVRRILAPIGRRLDESNPMVDARLPDGGRINVVIPPLALEGPVVS
HHCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHH
IRKFRKDPMTPADLQGLGTMSPEICDLLQAAVQARCNILVSGGTSSGKTSLLNALATFVP
HHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHCC
TTERVITIEDTAELALNHPHVVRLESRPGGFEGTGVVSIRDLLRNSLRMRPDRIIVGEVR
CCCEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
GGEVLEMLQAMSTGHDGSMGTIHASSPRECLYRLEMLAGFAGFQGSEASLRRQIANAIDF
CCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IVQIARLSNGKRRIVSITEITGLGDNIISTQELYRHEPYVNPDGTETDRWLSLGLLPHSP
HHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
KLARMRGPGFMLDDRFDV
CHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]