Click here to switch to the map view.

The map label for this gene is acoC

Identifier: 116694106

GI number: 116694106

Start: 170163

End: 171287

Strand: Direct

Name: acoC

Synonym: H16_B0146

Alternate gene names: 116694106

Gene position: 170163-171287 (Clockwise)

Preceding gene: 116694105

Following gene: 116694107

Centisome position: 5.84

GC content: 67.73

Gene sequence:

>1125_bases
ATGGCAACCGAAATTTCCCCCACGATTATCCCGATCGTGATGCCCAAGTGGGGCCTGTCGATGAAGGAAGGCACGGTCAA
TGCGTGGCTGGTCGACGAAGGCACCGAGATTACCGTGGGCCTGCCGATCCTGGATGTGGAAACCGACAAGATCGCCAATG
CGGTGGAGGCGCCCGACGCCGGCACGCTGCGCCGCAAGGTGGCGCAGGCCGGCGACGTGCTGCCGGTGAAGGCGCTGCTG
GGCGTGCTGGCACCTGCGGAGGTGAGCGATGCGCAAATCGATGACTATGTCGCGGCTTATGAAACGCCGGCGGACGACGC
TGGCGAAGAGGACGCCGCTGCCGCGTACCAGTTTGCCGACGTCGACGGCATCCGGGTCCGCTATGCCCGCAAGGGTGGCG
GCGCCGAAACCGTGCTCTTCATCCACGGATTTGGCGGAGACCTGGACAACTGGCTGTTCAACCTCGATCCCCTGGCCGAC
GCGTACACCGTGGTGGCGCTCGACCTGCCCGGCCACGGACAGTCGTCGCCGCGGCTCGCGGGCACGACGCTGGCGCAGAT
GGCCGGCTTTGTAGCGCGCTTCATGGACGAGACCGGGATCGAGGCGGCGCATGTGGTCGGCCATTCGATGGGCGGCGGCG
TGGCGGCGCAACTGGCCGTGGATGCACCGCAGCGGGTGCTGTCGGTGGCGCTGGTGTCGCCGGTGGGCTTTGGCGACGCC
GTCAACAGCGGCTATACCGAAGGCTTCGTCAGTGCCCAGTCCCGGCGCGAGCTCAAGCCCGTGGTCGAACTGCTGTTCGC
CGACGCGGGGCTGGTCAGCCGCCAGATGCTGGACGACCTGCTGCGCTACAAGCGGCTGGACGGCGTCACCGAGGCGCTGA
CGGCGCTGGGGCAGGGACTCTTCGGCGGCGGCCGCCAGAGCGAGCAGCCTGGCCAGCGGCTGGCCAACAGCGGCAAGCGC
GTGCTGGTGGTGTGGGGCGGTCAGGACCAGATCATCCCGGCCGCGCACGCTGAAGCCGCGCCGCCGGGAGCAACCGTCAA
GGTCTTTGCCGATGCTGGCCACATGAGCCAGATGGAGAAGGCCAATGACTTCAACGCGCTGCTGAAGAAGCATCTGGGCG
GCTGA

Upstream 100 bases:

>100_bases
CGCCGGTGCCGTTCTCGCCGACGCTGGAGGACCTGTACATCCCCAGCGCCGCGCAGATCGCGGCCGCCGCGCGCAAGACC
ATGAAGGGAGGCAAGCACTG

Downstream 100 bases:

>100_bases
TGCCGCGCGCGGATGGCGCCGCCCAGGGCGCCATCCGCTCGGCCAGCTCGCCTTGTTCCAATTCAAGACAGGAGACCTCA
TGACCACAGACCTGAAGACC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system; Fast-migrating protein; FMP

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MATEISPTIIPIVMPKWGLSMKEGTVNAWLVDEGTEITVGLPILDVETDKIANAVEAPDAGTLRRKVAQAGDVLPVKALL
GVLAPAEVSDAQIDDYVAAYETPADDAGEEDAAAAYQFADVDGIRVRYARKGGGAETVLFIHGFGGDLDNWLFNLDPLAD
AYTVVALDLPGHGQSSPRLAGTTLAQMAGFVARFMDETGIEAAHVVGHSMGGGVAAQLAVDAPQRVLSVALVSPVGFGDA
VNSGYTEGFVSAQSRRELKPVVELLFADAGLVSRQMLDDLLRYKRLDGVTEALTALGQGLFGGGRQSEQPGQRLANSGKR
VLVVWGGQDQIIPAAHAEAAPPGATVKVFADAGHMSQMEKANDFNALLKKHLGG

Sequences:

>Translated_374_residues
MATEISPTIIPIVMPKWGLSMKEGTVNAWLVDEGTEITVGLPILDVETDKIANAVEAPDAGTLRRKVAQAGDVLPVKALL
GVLAPAEVSDAQIDDYVAAYETPADDAGEEDAAAAYQFADVDGIRVRYARKGGGAETVLFIHGFGGDLDNWLFNLDPLAD
AYTVVALDLPGHGQSSPRLAGTTLAQMAGFVARFMDETGIEAAHVVGHSMGGGVAAQLAVDAPQRVLSVALVSPVGFGDA
VNSGYTEGFVSAQSRRELKPVVELLFADAGLVSRQMLDDLLRYKRLDGVTEALTALGQGLFGGGRQSEQPGQRLANSGKR
VLVVWGGQDQIIPAAHAEAAPPGATVKVFADAGHMSQMEKANDFNALLKKHLGG
>Mature_373_residues
ATEISPTIIPIVMPKWGLSMKEGTVNAWLVDEGTEITVGLPILDVETDKIANAVEAPDAGTLRRKVAQAGDVLPVKALLG
VLAPAEVSDAQIDDYVAAYETPADDAGEEDAAAAYQFADVDGIRVRYARKGGGAETVLFIHGFGGDLDNWLFNLDPLADA
YTVVALDLPGHGQSSPRLAGTTLAQMAGFVARFMDETGIEAAHVVGHSMGGGVAAQLAVDAPQRVLSVALVSPVGFGDAV
NSGYTEGFVSAQSRRELKPVVELLFADAGLVSRQMLDDLLRYKRLDGVTEALTALGQGLFGGGRQSEQPGQRLANSGKRV
LVVWGGQDQIIPAAHAEAAPPGATVKVFADAGHMSQMEKANDFNALLKKHLGG

Specific function: Dihydrolipoamide acetyltransferase involved in acetoin catabolism

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Escherichia coli, GI87081721, Length=281, Percent_Identity=27.7580071174377, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32566936, Length=106, Percent_Identity=35.8490566037736, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17558492, Length=106, Percent_Identity=35.8490566037736, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACOC_CUPNH (P27747)

Other databases:

- EMBL:   M66060
- EMBL:   AM260480
- RefSeq:   YP_728317.1
- ProteinModelPortal:   P27747
- SMR:   P27747
- STRING:   P27747
- MEROPS:   S33.010
- GeneID:   4454951
- GenomeReviews:   AM260480_GR
- KEGG:   reh:H16_B0146
- eggNOG:   COG0596
- HOGENOM:   HBG334443
- OMA:   CEVAPNR
- ProtClustDB:   PRK14875
- BioCyc:   REUT381666:H16_B0146-MONOMER
- InterPro:   IPR003016
- InterPro:   IPR000073
- InterPro:   IPR000089
- InterPro:   IPR011053
- PRINTS:   PR00111

Pfam domain/function: PF00561 Abhydrolase_1; PF00364 Biotin_lipoyl; SSF51230 Hybrid_motif

EC number: =2.3.1.12

Molecular weight: Translated: 39136; Mature: 39005

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50968 BIOTINYL_LIPOYL; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEISPTIIPIVMPKWGLSMKEGTVNAWLVDEGTEITVGLPILDVETDKIANAVEAPDA
CCCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCEEEEECEEEECCHHHHHHHHCCCCC
GTLRRKVAQAGDVLPVKALLGVLAPAEVSDAQIDDYVAAYETPADDAGEEDAAAAYQFAD
HHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHEEEEC
VDGIRVRYARKGGGAETVLFIHGFGGDLDNWLFNLDPLADAYTVVALDLPGHGQSSPRLA
CCCEEEEEEECCCCCEEEEEEEECCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHH
GTTLAQMAGFVARFMDETGIEAAHVVGHSMGGGVAAQLAVDAPQRVLSVALVSPVGFGDA
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHH
VNSGYTEGFVSAQSRRELKPVVELLFADAGLVSRQMLDDLLRYKRLDGVTEALTALGQGL
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
FGGGRQSEQPGQRLANSGKRVLVVWGGQDQIIPAAHAEAAPPGATVKVFADAGHMSQMEK
CCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
ANDFNALLKKHLGG
HHHHHHHHHHHHCC
>Mature Secondary Structure 
ATEISPTIIPIVMPKWGLSMKEGTVNAWLVDEGTEITVGLPILDVETDKIANAVEAPDA
CCCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCEEEEECEEEECCHHHHHHHHCCCCC
GTLRRKVAQAGDVLPVKALLGVLAPAEVSDAQIDDYVAAYETPADDAGEEDAAAAYQFAD
HHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHEEEEC
VDGIRVRYARKGGGAETVLFIHGFGGDLDNWLFNLDPLADAYTVVALDLPGHGQSSPRLA
CCCEEEEEEECCCCCEEEEEEEECCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHH
GTTLAQMAGFVARFMDETGIEAAHVVGHSMGGGVAAQLAVDAPQRVLSVALVSPVGFGDA
HHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCHH
VNSGYTEGFVSAQSRRELKPVVELLFADAGLVSRQMLDDLLRYKRLDGVTEALTALGQGL
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
FGGGRQSEQPGQRLANSGKRVLVVWGGQDQIIPAAHAEAAPPGATVKVFADAGHMSQMEK
CCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
ANDFNALLKKHLGG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286