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The map label for this gene is glnG [H]

Identifier: 116694039

GI number: 116694039

Start: 90201

End: 91094

Strand: Direct

Name: glnG [H]

Synonym: H16_B0079

Alternate gene names: 116694039

Gene position: 90201-91094 (Clockwise)

Preceding gene: 116694038

Following gene: 116694042

Centisome position: 3.1

GC content: 68.68

Gene sequence:

>894_bases
ATGCATCCCGAACCTGACACCTTTCGCCCGTTGCCGACGATCGAGACCCGCGTACTGGTGGTCGACGACGATGCCGACGC
GTGCGGCTTCCTGACGCGCGCGCTGGGTGGCGAAGCCATGCTGGTCCACACCGCCGCGAACGGCAACGCGGCACTGGATG
CGCTGACGTCCTTCGCGCCGGATGCGGTGCTGCTGGACGTGGACATGCCGGGCATGGACGGCTTTGCCGCCTGCACGGCG
CTGCTGGACCGCCACGCGGACCTGCCGGTGATCTTCATGACCGGACTTGGCGATACCGGTCATATCGTGCGCGGTTTCGA
AGTCGGCGCATGCGACTACGTGACCAAACCGGTGGCGATTGCCGAGGTGGTGGCGCGCGTGCGGTCTCATGCGGGCAAGG
CACGGCTGGCGCGCAGCGCACGGGAGGCCGTATTCGGCAGCACGGTGCCGATGCTCGCGGCCGGCATCGACGGCCGGCTC
CTGTGGATAAACGGCAGCGCGTCAGCCTTGCTGCAGGCCCAGGGATGGCCGGTAGCGATTGCCGGCGCGGTACTGCCTCC
CGCGCTGCGCGACGCGGTGTTCCGCCTCTGCAGCGAAGCGGAAACTGGCGCTGATAGTCTTCACCTGCGCCGGTTGACAG
GCGAGGCGGGCGACGTGGTCGTGGTGGAACTGTCGTGCAGCGGTCTGGCCCCGGCCGGCCCGGCCCTGACACTGCGCGAG
GCCGAGGTGATGGCCTGGGTGGCGCGCGGCAAGACCAATCGCGATATCGCCGACATCCTCGGCATGAGCCCGCGCACGGT
GAACAAGCACCTGGAACACATCTACGAGAAGCTCGGCGTGGAAACGCGAACGGCGGCAGTCGCCGCCACCCGGCGCAACT
TGGGGACGGCGTGA

Upstream 100 bases:

>100_bases
GCGGAAGGCGGCGGCGCCGTGGCCACGTGGGCACTGCAGATGCTGGCGCTCGCGCGGACCGATGGCACTGCCCTGACCGC
ATCACTTGGCGAGGCGCTCC

Downstream 100 bases:

>100_bases
CGTGCCGTGCGCCAAGGCAAGAAGGGCCGGCGTTGCCGGCCCTTCTTGCCAGTCGGATCCCGATCAGAAGCGGTAACCCA
CCCCCATGCTGAACAGCCAC

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MHPEPDTFRPLPTIETRVLVVDDDADACGFLTRALGGEAMLVHTAANGNAALDALTSFAPDAVLLDVDMPGMDGFAACTA
LLDRHADLPVIFMTGLGDTGHIVRGFEVGACDYVTKPVAIAEVVARVRSHAGKARLARSAREAVFGSTVPMLAAGIDGRL
LWINGSASALLQAQGWPVAIAGAVLPPALRDAVFRLCSEAETGADSLHLRRLTGEAGDVVVVELSCSGLAPAGPALTLRE
AEVMAWVARGKTNRDIADILGMSPRTVNKHLEHIYEKLGVETRTAAVAATRRNLGTA

Sequences:

>Translated_297_residues
MHPEPDTFRPLPTIETRVLVVDDDADACGFLTRALGGEAMLVHTAANGNAALDALTSFAPDAVLLDVDMPGMDGFAACTA
LLDRHADLPVIFMTGLGDTGHIVRGFEVGACDYVTKPVAIAEVVARVRSHAGKARLARSAREAVFGSTVPMLAAGIDGRL
LWINGSASALLQAQGWPVAIAGAVLPPALRDAVFRLCSEAETGADSLHLRRLTGEAGDVVVVELSCSGLAPAGPALTLRE
AEVMAWVARGKTNRDIADILGMSPRTVNKHLEHIYEKLGVETRTAAVAATRRNLGTA
>Mature_297_residues
MHPEPDTFRPLPTIETRVLVVDDDADACGFLTRALGGEAMLVHTAANGNAALDALTSFAPDAVLLDVDMPGMDGFAACTA
LLDRHADLPVIFMTGLGDTGHIVRGFEVGACDYVTKPVAIAEVVARVRSHAGKARLARSAREAVFGSTVPMLAAGIDGRL
LWINGSASALLQAQGWPVAIAGAVLPPALRDAVFRLCSEAETGADSLHLRRLTGEAGDVVVVELSCSGLAPAGPALTLRE
AEVMAWVARGKTNRDIADILGMSPRTVNKHLEHIYEKLGVETRTAAVAATRRNLGTA

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=41.7322834645669, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=35.1851851851852, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790860, Length=112, Percent_Identity=37.5, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789809, Length=113, Percent_Identity=33.6283185840708, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786784, Length=113, Percent_Identity=32.7433628318584, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=29.1338582677165, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=33.9130434782609, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787229, Length=124, Percent_Identity=30.6451612903226, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788192, Length=135, Percent_Identity=35.5555555555556, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 31101; Mature: 31101

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPEPDTFRPLPTIETRVLVVDDDADACGFLTRALGGEAMLVHTAANGNAALDALTSFAP
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCC
DAVLLDVDMPGMDGFAACTALLDRHADLPVIFMTGLGDTGHIVRGFEVGACDYVTKPVAI
CEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEECEECCCHHHHHCHHHH
AEVVARVRSHAGKARLARSAREAVFGSTVPMLAAGIDGRLLWINGSASALLQAQGWPVAI
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEEECCCCEEE
AGAVLPPALRDAVFRLCSEAETGADSLHLRRLTGEAGDVVVVELSCSGLAPAGPALTLRE
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCEEEEH
AEVMAWVARGKTNRDIADILGMSPRTVNKHLEHIYEKLGVETRTAAVAATRRNLGTA
HHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHPEPDTFRPLPTIETRVLVVDDDADACGFLTRALGGEAMLVHTAANGNAALDALTSFAP
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCC
DAVLLDVDMPGMDGFAACTALLDRHADLPVIFMTGLGDTGHIVRGFEVGACDYVTKPVAI
CEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEECEECCCHHHHHCHHHH
AEVVARVRSHAGKARLARSAREAVFGSTVPMLAAGIDGRLLWINGSASALLQAQGWPVAI
HHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEEECCCCEEE
AGAVLPPALRDAVFRLCSEAETGADSLHLRRLTGEAGDVVVVELSCSGLAPAGPALTLRE
ECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCCEEEEH
AEVMAWVARGKTNRDIADILGMSPRTVNKHLEHIYEKLGVETRTAAVAATRRNLGTA
HHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]