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The map label for this gene is livJ [C]

Identifier: 116694021

GI number: 116694021

Start: 70092

End: 71228

Strand: Direct

Name: livJ [C]

Synonym: H16_B0061

Alternate gene names: 116694021

Gene position: 70092-71228 (Clockwise)

Preceding gene: 116694020

Following gene: 116694022

Centisome position: 2.41

GC content: 68.07

Gene sequence:

>1137_bases
ATGAAAGCGAAATGGATCGCGTGCGGCATGCTGGCCGCCACGCTGGGATGGGCCGCACAGACGGCCCTGGCGGAACCGGG
CGTGACCCGCAACACCATCCGCATCGGCCAGTCGGCCGGCGTCACCGGTCCGGTGGCGGGTTCGGTCAAGGAGCAGATCG
CCGGCGCGCAGGTGTACCTGCGCACCATCAACGCCAGCGGCGGCGTGGCCGGGCGGCGCATCGAGCTGGTGACCCACGAT
GACGGCTTCGATGCCAAGCGCACGCCCGACAATGTGCGCAAGCTGATCGACGAGGACAAGGTGTTCGCGCTCTTCATGGT
GCGCGGCACGCCGCAGAACGAAAGCATCCTGCCCATCATCGCCGCGCAGAAGGTGCCGCTGGTGGCGCCGCTGACCGGCG
CCATCACGCTGCACCGGCCGGTCAACCGCTACGTGTTCAATGTGCGCGCCAAGTACCAGGACGAGGTGGCGCGCGCGATC
AACCACCTGGCCACCTCGGGCATGAGCCGCATCGGCATCTTCTACGCCAACGACGGTTTTGGCCGGGACGTGCTCGAAGG
CTACAGCGCCGCGCTGCAGGCGCGCGGGGTGCAGCCGGCGGCGACGGCAAGCTTTGCCCGGCCCATGGGCGATATCACCC
AGAGCGTGGCCGCCATGAACAAGGCCAACCCGCAGGCGGTACTGGTGATCGGCTCGGGCTCCGAGGCGGCGCGCATCATC
CGCGAACTGCGCCGCGCAGGCAGCGAGGCACAGTTCGTCACGCTGTCCAACAACGCCGCCGATTCCTTTATCCGTGAACT
GGGCGACGATGCGCGCGGGCTGATCATCACGCAGGTGGTGCCGGGCACCAACTCCAGCCAGATGACGCTGGCCAGCGAAT
ACCGCAGCCTGGCCAAGCAGCAGGGCGTGGAGCCGTCCAACGCCGGCATGGAGGGCTTCATGTCGGCCAAGGTGCTGGTC
GAAGGGCTGCGCCGCGCCGGCCCCGACCTGACGCGCGAGCGCCTGGTCACGGCGCTGGAAGGCATGCGCGACTACGACCT
TGGCGGCATCCTGATCAGCTACAGCGCCGAGCGCCATACCGGCTCGTCGTTCGTGGAAATGTCGATCGTCTCGTCCACCG
GCAAGCTGATCCGCTGA

Upstream 100 bases:

>100_bases
ATGAGACGCATGCGGTAACTTGCGTAACGGCGCGCGGCCGTGCATGCCGCGCCGCCATACACAATGAAGGACAGCCGCAC
AGGCGCAAGGGGTCGAGACA

Downstream 100 bases:

>100_bases
AAGCCGCGGATAGGGGGGCTGCGGTATAGTCCCCCGCATGGCTACTCCTACCCTCTCCCCCGATTCCCGTACCCCGTTCT
CGCTGCACGGCCGCGTGGCG

Product: ABC-type transporter, periplasmic component

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKAKWIACGMLAATLGWAAQTALAEPGVTRNTIRIGQSAGVTGPVAGSVKEQIAGAQVYLRTINASGGVAGRRIELVTHD
DGFDAKRTPDNVRKLIDEDKVFALFMVRGTPQNESILPIIAAQKVPLVAPLTGAITLHRPVNRYVFNVRAKYQDEVARAI
NHLATSGMSRIGIFYANDGFGRDVLEGYSAALQARGVQPAATASFARPMGDITQSVAAMNKANPQAVLVIGSGSEAARII
RELRRAGSEAQFVTLSNNAADSFIRELGDDARGLIITQVVPGTNSSQMTLASEYRSLAKQQGVEPSNAGMEGFMSAKVLV
EGLRRAGPDLTRERLVTALEGMRDYDLGGILISYSAERHTGSSFVEMSIVSSTGKLIR

Sequences:

>Translated_378_residues
MKAKWIACGMLAATLGWAAQTALAEPGVTRNTIRIGQSAGVTGPVAGSVKEQIAGAQVYLRTINASGGVAGRRIELVTHD
DGFDAKRTPDNVRKLIDEDKVFALFMVRGTPQNESILPIIAAQKVPLVAPLTGAITLHRPVNRYVFNVRAKYQDEVARAI
NHLATSGMSRIGIFYANDGFGRDVLEGYSAALQARGVQPAATASFARPMGDITQSVAAMNKANPQAVLVIGSGSEAARII
RELRRAGSEAQFVTLSNNAADSFIRELGDDARGLIITQVVPGTNSSQMTLASEYRSLAKQQGVEPSNAGMEGFMSAKVLV
EGLRRAGPDLTRERLVTALEGMRDYDLGGILISYSAERHTGSSFVEMSIVSSTGKLIR
>Mature_378_residues
MKAKWIACGMLAATLGWAAQTALAEPGVTRNTIRIGQSAGVTGPVAGSVKEQIAGAQVYLRTINASGGVAGRRIELVTHD
DGFDAKRTPDNVRKLIDEDKVFALFMVRGTPQNESILPIIAAQKVPLVAPLTGAITLHRPVNRYVFNVRAKYQDEVARAI
NHLATSGMSRIGIFYANDGFGRDVLEGYSAALQARGVQPAATASFARPMGDITQSVAAMNKANPQAVLVIGSGSEAARII
RELRRAGSEAQFVTLSNNAADSFIRELGDDARGLIITQVVPGTNSSQMTLASEYRSLAKQQGVEPSNAGMEGFMSAKVLV
EGLRRAGPDLTRERLVTALEGMRDYDLGGILISYSAERHTGSSFVEMSIVSSTGKLIR

Specific function: This Protein Is A Component Of The Leucine, Isoleucine, Valine, (Threonine) Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40261; Mature: 40261

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKWIACGMLAATLGWAAQTALAEPGVTRNTIRIGQSAGVTGPVAGSVKEQIAGAQVYL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCEEEE
RTINASGGVAGRRIELVTHDDGFDAKRTPDNVRKLIDEDKVFALFMVRGTPQNESILPII
EEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEE
AAQKVPLVAPLTGAITLHRPVNRYVFNVRAKYQDEVARAINHLATSGMSRIGIFYANDGF
ECCCCCEEECCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GRDVLEGYSAALQARGVQPAATASFARPMGDITQSVAAMNKANPQAVLVIGSGSEAARII
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
RELRRAGSEAQFVTLSNNAADSFIRELGDDARGLIITQVVPGTNSSQMTLASEYRSLAKQ
HHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHH
QGVEPSNAGMEGFMSAKVLVEGLRRAGPDLTRERLVTALEGMRDYDLGGILISYSAERHT
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
GSSFVEMSIVSSTGKLIR
CCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKAKWIACGMLAATLGWAAQTALAEPGVTRNTIRIGQSAGVTGPVAGSVKEQIAGAQVYL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHCCCEEEE
RTINASGGVAGRRIELVTHDDGFDAKRTPDNVRKLIDEDKVFALFMVRGTPQNESILPII
EEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEE
AAQKVPLVAPLTGAITLHRPVNRYVFNVRAKYQDEVARAINHLATSGMSRIGIFYANDGF
ECCCCCEEECCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GRDVLEGYSAALQARGVQPAATASFARPMGDITQSVAAMNKANPQAVLVIGSGSEAARII
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHH
RELRRAGSEAQFVTLSNNAADSFIRELGDDARGLIITQVVPGTNSSQMTLASEYRSLAKQ
HHHHHCCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEHHHHHHHHHHH
QGVEPSNAGMEGFMSAKVLVEGLRRAGPDLTRERLVTALEGMRDYDLGGILISYSAERHT
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
GSSFVEMSIVSSTGKLIR
CCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA