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The map label for this gene is gcvH

Identifier: 113478356

GI number: 113478356

Start: 7691370

End: 7691765

Strand: Reverse

Name: gcvH

Synonym: Tery_5032

Alternate gene names: 113478356

Gene position: 7691765-7691370 (Counterclockwise)

Preceding gene: 113478358

Following gene: 113478355

Centisome position: 99.25

GC content: 38.13

Gene sequence:

>396_bases
ATGACACTAGAATTTCCAGATGACCTTAAATATTCTGACTCCCATGAATATGTGAGGATGGATGGGGAAATTGCCACTGT
TGGTATTAGTGCCTATGCTATCGACCAGTTGGGGGATATTGTATTTATCGAGCTTCCAGAAGTGGGAGACAGTATAGAAA
AAGAAGAACCATTTGGCACTATTGAATCTGTGAAAGCAGTGGAAGATCTTAAAGCTCCTGTCACTGGTACAGTAATAGAG
CGTAATGAAGATATATTAGATGAGCATGAGAAATTATCAGAAGACCCATACATAGAAGGTTGGTTATTGAAAGTGCGTGT
TAAAGACCCTGATGAATTAAATGGAGATGATATTTACTCTGCTGATGATTATCGTAAACTTGTAGAAGGAGAGTAA

Upstream 100 bases:

>100_bases
TTACCTTATATTATTCTTTATGGAATAATTTTTAATCCAAGTCCAGTCTTGATCTGACTGGCATAATTCATATTATTGTA
GTGAGTAGTGAGGAATATAA

Downstream 100 bases:

>100_bases
TTAACAAGAAGACCTATACATATAAGGTTGGTTATTGAAAGTGTGTGTTAAAGACCCTAATGAATTAGATGGAGATGATG
TTTTCTCTGCTGACAAGTAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MTLEFPDDLKYSDSHEYVRMDGEIATVGISAYAIDQLGDIVFIELPEVGDSIEKEEPFGTIESVKAVEDLKAPVTGTVIE
RNEDILDEHEKLSEDPYIEGWLLKVRVKDPDELNGDDIYSADDYRKLVEGE

Sequences:

>Translated_131_residues
MTLEFPDDLKYSDSHEYVRMDGEIATVGISAYAIDQLGDIVFIELPEVGDSIEKEEPFGTIESVKAVEDLKAPVTGTVIE
RNEDILDEHEKLSEDPYIEGWLLKVRVKDPDELNGDDIYSADDYRKLVEGE
>Mature_130_residues
TLEFPDDLKYSDSHEYVRMDGEIATVGISAYAIDQLGDIVFIELPEVGDSIEKEEPFGTIESVKAVEDLKAPVTGTVIER
NEDILDEHEKLSEDPYIEGWLLKVRVKDPDELNGDDIYSADDYRKLVEGE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=39.1666666666667, Blast_Score=99, Evalue=1e-21,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=38.3333333333333, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=38.5826771653543, Blast_Score=93, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=41.025641025641, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=117, Percent_Identity=39.3162393162393, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=103, Percent_Identity=39.8058252427184, Blast_Score=84, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17865652, Length=129, Percent_Identity=37.2093023255814, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_TRIEI (Q10UY0)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_724417.1
- ProteinModelPortal:   Q10UY0
- SMR:   Q10UY0
- STRING:   Q10UY0
- GeneID:   4246687
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_5032
- NMPDR:   fig|203124.1.peg.3970
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   TERY203124:TERY_5032-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14795; Mature: 14664

Theoretical pI: Translated: 3.83; Mature: 3.83

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEFPDDLKYSDSHEYVRMDGEIATVGISAYAIDQLGDIVFIELPEVGDSIEKEEPFGT
CCCCCCCCCCCCCCCCEEEECCCEEEEEEHHEEHHCCCCEEEEECCCCCCCCCCCCCCCH
IESVKAVEDLKAPVTGTVIERNEDILDEHEKLSEDPYIEGWLLKVRVKDPDELNGDDIYS
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCC
ADDYRKLVEGE
CHHHHHHHCCC
>Mature Secondary Structure 
TLEFPDDLKYSDSHEYVRMDGEIATVGISAYAIDQLGDIVFIELPEVGDSIEKEEPFGT
CCCCCCCCCCCCCCCEEEECCCEEEEEEHHEEHHCCCCEEEEECCCCCCCCCCCCCCCH
IESVKAVEDLKAPVTGTVIERNEDILDEHEKLSEDPYIEGWLLKVRVKDPDELNGDDIYS
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCC
ADDYRKLVEGE
CHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA