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The map label for this gene is trmE

Identifier: 113478139

GI number: 113478139

Start: 7336544

End: 7337947

Strand: Reverse

Name: trmE

Synonym: Tery_4774

Alternate gene names: 113478139

Gene position: 7337947-7336544 (Counterclockwise)

Preceding gene: 113478142

Following gene: 113478138

Centisome position: 94.68

GC content: 39.96

Gene sequence:

>1404_bases
ATGGTGATAACTGAAATGACTCCTAGAAATACTATTGCCGCGATCGCTACTGCTATTGTTCCCCAACAAGGAAGTGTGGG
GATAGTCCGAATGTCTGGTTCTGAGGCGATGAAAATTGCTCAAACCCTATTTCATGCACCGGGGAAGCAAGTTTGGGAGA
CTCACCATATTCTTTATGGCTATATCCGTCATCCCCACACTGGGCAATTAGTAGATGAAACTTTATTATTGATTATGAAA
GCTCCTCGTTCTTATACCCGTGAGGATGTGGTAGAATTCCATTGTCATGGTGGAATTATTGCGATACAACAAGTATTACA
ATTATGTATTGAGGCAGGGGCAGAGCTAGCTCAACCAGGAGAGTTTACTTTACGGGCTTTCTTAAATGGACGATTGGATC
TGACTCAAGCTGAAAGTATTGCAGAACTCGTAGGTTCTCAATCTCCCGCTGCTGCTCAAGTTGCTTTAGCTGGTTTGCAG
GGTAAGTTAGCTAGTCCAATTCGTCACTTAAGAGCTTGTTGTCTAGATATTTTAGCAGAAATAGAGGCACGCATTGATTT
TGAGGAAGATTTACCTCCTTTGGATGAATCTGAAATTAGTCAAAAACTGGATGATATTTTGGTGGATTTGTCAATGGTAT
TAGCTACAGCAAGTCGGGGAGAGTTATTACGTACTGGTTTAAAGGTCGCTATTATCGGACGTCCGAATGTGGGCAAGTCG
AGTTTGTTAAATGCTTGGAGTCATAGCGATCGCGCTATTGTTACCGATCTTCCTGGTACAACCAGGGATGTAGTTGAATC
TCAGTTGGTGGTAGGTGGTATTCCGGTTCAGGTTTTAGATACAGCCGGTATTCGGGAAACTGAGGATCGGGTAGAAAAAA
TTGGGGTAGAGCGATCGTGTCAGGCAGCTGAGTCTGCTGACATTGTTTTATTGACGATTGACGCTCAGGTTGGTTGGACG
GAGTTAGATGAAGTTATTTATCAACAGGTCAAACATCGTTTTTTAATTTTGATAATTAATAAAGTTGATCAAGTTGATTT
GGTTCATTCAGAATTAATTAGGTCAATTTTTTACCCAGAAACTATTAAAAATGTTGTGGCTACAGCCGCTATTAATAATC
AAGGAATTGAGGAACTGGAAGCAGCAATTCTTAATGCTGTAAATTTAGATAATGTGCAGCCAGAAAATTTAGATTTTGCA
GTTAATCAAAGGCAAGCAGCAGCTTTAACTAGAGCTAAGATAGCTTTGGAGCAATGTTTAAATACTATTAAAAATAACTT
ACCTTTAGATTTTTGGACAATCGACTTAAGGGGAAGCATTTATGCTTTGGGAGAGGTTACGGGAGAGGATGTAACGGAAT
CTGTTTTGGATATAATTTTTAGTCGCTTTTGTATTGGGAAGTAA

Upstream 100 bases:

>100_bases
CAGAATTATAAGCCCCCCCCTTTTGAAAGAATCAAAAAAATCAAATTCAGTATTCTAAGCCGGAAGCTTCAGCGGTTGGT
ACTGTAATGGTGATAACTGT

Downstream 100 bases:

>100_bases
TTTTTTTTTGATAGTTAAGTAGGGATGTTGCATCGAAGGTCCATATTTTTCGATAATTATTTTAGAACATTTGAGAACAG
TAGTAAGAGCATCAAGCTCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVITEMTPRNTIAAIATAIVPQQGSVGIVRMSGSEAMKIAQTLFHAPGKQVWETHHILYGYIRHPHTGQLVDETLLLIMK
APRSYTREDVVEFHCHGGIIAIQQVLQLCIEAGAELAQPGEFTLRAFLNGRLDLTQAESIAELVGSQSPAAAQVALAGLQ
GKLASPIRHLRACCLDILAEIEARIDFEEDLPPLDESEISQKLDDILVDLSMVLATASRGELLRTGLKVAIIGRPNVGKS
SLLNAWSHSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETEDRVEKIGVERSCQAAESADIVLLTIDAQVGWT
ELDEVIYQQVKHRFLILIINKVDQVDLVHSELIRSIFYPETIKNVVATAAINNQGIEELEAAILNAVNLDNVQPENLDFA
VNQRQAAALTRAKIALEQCLNTIKNNLPLDFWTIDLRGSIYALGEVTGEDVTESVLDIIFSRFCIGK

Sequences:

>Translated_467_residues
MVITEMTPRNTIAAIATAIVPQQGSVGIVRMSGSEAMKIAQTLFHAPGKQVWETHHILYGYIRHPHTGQLVDETLLLIMK
APRSYTREDVVEFHCHGGIIAIQQVLQLCIEAGAELAQPGEFTLRAFLNGRLDLTQAESIAELVGSQSPAAAQVALAGLQ
GKLASPIRHLRACCLDILAEIEARIDFEEDLPPLDESEISQKLDDILVDLSMVLATASRGELLRTGLKVAIIGRPNVGKS
SLLNAWSHSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETEDRVEKIGVERSCQAAESADIVLLTIDAQVGWT
ELDEVIYQQVKHRFLILIINKVDQVDLVHSELIRSIFYPETIKNVVATAAINNQGIEELEAAILNAVNLDNVQPENLDFA
VNQRQAAALTRAKIALEQCLNTIKNNLPLDFWTIDLRGSIYALGEVTGEDVTESVLDIIFSRFCIGK
>Mature_467_residues
MVITEMTPRNTIAAIATAIVPQQGSVGIVRMSGSEAMKIAQTLFHAPGKQVWETHHILYGYIRHPHTGQLVDETLLLIMK
APRSYTREDVVEFHCHGGIIAIQQVLQLCIEAGAELAQPGEFTLRAFLNGRLDLTQAESIAELVGSQSPAAAQVALAGLQ
GKLASPIRHLRACCLDILAEIEARIDFEEDLPPLDESEISQKLDDILVDLSMVLATASRGELLRTGLKVAIIGRPNVGKS
SLLNAWSHSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETEDRVEKIGVERSCQAAESADIVLLTIDAQVGWT
ELDEVIYQQVKHRFLILIINKVDQVDLVHSELIRSIFYPETIKNVVATAAINNQGIEELEAAILNAVNLDNVQPENLDFA
VNQRQAAALTRAKIALEQCLNTIKNNLPLDFWTIDLRGSIYALGEVTGEDVTESVLDIIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=484, Percent_Identity=33.2644628099174, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI193082993, Length=473, Percent_Identity=34.0380549682875, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI193082988, Length=516, Percent_Identity=31.2015503875969, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI2367268, Length=472, Percent_Identity=35.1694915254237, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI87082120, Length=158, Percent_Identity=36.0759493670886, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=30.0865800865801, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=509, Percent_Identity=27.8978388998035, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45550813, Length=494, Percent_Identity=30.3643724696356, Blast_Score=181, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_TRIEI (Q10VJ7)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_724200.1
- ProteinModelPortal:   Q10VJ7
- SMR:   Q10VJ7
- STRING:   Q10VJ7
- GeneID:   4246428
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_4774
- NMPDR:   fig|203124.1.peg.154
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q10VJ7
- ProtClustDB:   PRK05291
- BioCyc:   TERY203124:TERY_4774-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51044; Mature: 51044

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 92-92 BINDING 131-131 BINDING 467-467

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVITEMTPRNTIAAIATAIVPQQGSVGIVRMSGSEAMKIAQTLFHAPGKQVWETHHILYG
CEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHH
YIRHPHTGQLVDETLLLIMKAPRSYTREDVVEFHCHGGIIAIQQVLQLCIEAGAELAQPG
HHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
EFTLRAFLNGRLDLTQAESIAELVGSQSPAAAQVALAGLQGKLASPIRHLRACCLDILAE
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IEARIDFEEDLPPLDESEISQKLDDILVDLSMVLATASRGELLRTGLKVAIIGRPNVGKS
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCHH
SLLNAWSHSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETEDRVEKIGVERSC
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHH
QAAESADIVLLTIDAQVGWTELDEVIYQQVKHRFLILIINKVDQVDLVHSELIRSIFYPE
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCHH
TIKNVVATAAINNQGIEELEAAILNAVNLDNVQPENLDFAVNQRQAAALTRAKIALEQCL
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
NTIKNNLPLDFWTIDLRGSIYALGEVTGEDVTESVLDIIFSRFCIGK
HHHHHCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVITEMTPRNTIAAIATAIVPQQGSVGIVRMSGSEAMKIAQTLFHAPGKQVWETHHILYG
CEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHH
YIRHPHTGQLVDETLLLIMKAPRSYTREDVVEFHCHGGIIAIQQVLQLCIEAGAELAQPG
HHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCC
EFTLRAFLNGRLDLTQAESIAELVGSQSPAAAQVALAGLQGKLASPIRHLRACCLDILAE
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IEARIDFEEDLPPLDESEISQKLDDILVDLSMVLATASRGELLRTGLKVAIIGRPNVGKS
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCCHH
SLLNAWSHSDRAIVTDLPGTTRDVVESQLVVGGIPVQVLDTAGIRETEDRVEKIGVERSC
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHH
QAAESADIVLLTIDAQVGWTELDEVIYQQVKHRFLILIINKVDQVDLVHSELIRSIFYPE
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHCHH
TIKNVVATAAINNQGIEELEAAILNAVNLDNVQPENLDFAVNQRQAAALTRAKIALEQCL
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHH
NTIKNNLPLDFWTIDLRGSIYALGEVTGEDVTESVLDIIFSRFCIGK
HHHHHCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA