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The map label for this gene is lpdA [H]

Identifier: 113476711

GI number: 113476711

Start: 4839782

End: 4841212

Strand: Reverse

Name: lpdA [H]

Synonym: Tery_3171

Alternate gene names: 113476711

Gene position: 4841212-4839782 (Counterclockwise)

Preceding gene: 113476712

Following gene: 113476710

Centisome position: 62.47

GC content: 38.23

Gene sequence:

>1431_bases
ATGACTCAAGAATTTGATTACGACTTAATAATTATTGGTGCAGGTGTCGGTGGACATGGTGCAGCATTACACGCTACCAG
TTGTGGCCTAAAAACAGCTATTGTAGAAGTAGCAGAAATGGGAGGTACTTGTGTTAACCGAGGTTGTATACCATCTAAGG
CACTTCTCGCAGCATCAGGTAAAGTTCGAGAGTTACGAAATGCTCACCACTTAAAAACTTTGGGAATTGAGTTGGATAAT
GTTTCTTATGACAGACAAGTAATGGCCACTCATGCAAGTAACATTGTGACCAAAATTAGAGGTGACATGAGCAAAAGCCT
TAAACGTCTGAGTGTAGATATTATTACAGGGTGGGCTCAGGTAGCAGGAAAACAAAAAGTTACGGTTAAGACGGAAAAGG
GAGAAGAAAACTTTACTGCCAAAGATATTATACTTGCTCCTGGTTCAGTACCTTTTGTTCCTCCTGGAATAGAATTGGAT
GGTAAAACAGTATTTACCAGTGATGATGCTCTTAAACTAGACTGGTTACCACCTTGGGTTGCAATTATTGGTAGTGGTTA
TATAGGACTAGAATTTTCTGATATTTACACTGCCCTTGGATCTGAAATTACGATGATTGAGGCATTAGATAAGTTAATGC
CTACTTTCGATCCAGATATAGCTAAGATTGCACAAAGAGTTCTAATTCAGTCAAGAGATATTGAAGTAAAAGTAGGGAAG
TTGGCTATAAAGGTAGTTCCTGGATCTCCGGTAATTATTGAACTTGCCGATGCCAAGACTAAAGAAGTAGAAGAAATTAT
AGAGGTTGATGCTTGTCTAGTTGCCACAGGTCGCATTCCCTATACAAAAGATTTAGGACTAGATTCTGTAGCAGTAGAAA
CTGATAAATATGGATTTATTCCAGTAAATAGCAAAATGGCAGTTTTGTCAAGTGGTGAACCAGTACCTAATTTATGGGCA
ATTGGTGATGCAACAGGAAAAATGATGTTGGCTCATGCAGCATCTGCCCAAGGAATAACAGTGGTAGAAAATATATGTGG
TCGTGATCGAGAACCAGATTATCTTAGTATTCCGGCGGCAGCTTTTACTCATCCAGAAATTAGCTATGTTGGTATGACAG
AACCAGCAGCAAAAGATTTAGGCCAAAAACAGGGGTTTGAAGTGGCAAGTGTCAGAACTTATTTTAAGGGTAATTCTAAG
GCGATAGCTGAAGATGAAACAGATGGTATTGCTAAAGTAATTTATCGTCAAGATACAGGAGAATTATTAGGAGTACATAT
TATTGGTCTTCATGCCTCTGACTTAATTCAAGAAGCAGCAAATGCTATAGCTAAAAAACAATCTGTTAATGAGTTATCTT
TTAATGTACATACTCATCCTACTTTATCAGAAGTTTTGGATGAAGCATTTAAACGAGCCACTGTTCACTAG

Upstream 100 bases:

>100_bases
CTGTGAGTCAAATAAAACTCTAATAATATTTAGCTGCATTCCTAAGAAACCAAAAAAATATAGTTTTTGCAAACATACCT
AGAAGTTTTAGAAAACAGAA

Downstream 100 bases:

>100_bases
CTATTAGTATTAAGGAGATTAGTGGAATGGTAGATTCACTAAAATTAATACTATTAAAGCCCTAAAAATATAAGGTTAAA
ATAAAATAATAAATTACTAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTQEFDYDLIIIGAGVGGHGAALHATSCGLKTAIVEVAEMGGTCVNRGCIPSKALLAASGKVRELRNAHHLKTLGIELDN
VSYDRQVMATHASNIVTKIRGDMSKSLKRLSVDIITGWAQVAGKQKVTVKTEKGEENFTAKDIILAPGSVPFVPPGIELD
GKTVFTSDDALKLDWLPPWVAIIGSGYIGLEFSDIYTALGSEITMIEALDKLMPTFDPDIAKIAQRVLIQSRDIEVKVGK
LAIKVVPGSPVIIELADAKTKEVEEIIEVDACLVATGRIPYTKDLGLDSVAVETDKYGFIPVNSKMAVLSSGEPVPNLWA
IGDATGKMMLAHAASAQGITVVENICGRDREPDYLSIPAAAFTHPEISYVGMTEPAAKDLGQKQGFEVASVRTYFKGNSK
AIAEDETDGIAKVIYRQDTGELLGVHIIGLHASDLIQEAANAIAKKQSVNELSFNVHTHPTLSEVLDEAFKRATVH

Sequences:

>Translated_476_residues
MTQEFDYDLIIIGAGVGGHGAALHATSCGLKTAIVEVAEMGGTCVNRGCIPSKALLAASGKVRELRNAHHLKTLGIELDN
VSYDRQVMATHASNIVTKIRGDMSKSLKRLSVDIITGWAQVAGKQKVTVKTEKGEENFTAKDIILAPGSVPFVPPGIELD
GKTVFTSDDALKLDWLPPWVAIIGSGYIGLEFSDIYTALGSEITMIEALDKLMPTFDPDIAKIAQRVLIQSRDIEVKVGK
LAIKVVPGSPVIIELADAKTKEVEEIIEVDACLVATGRIPYTKDLGLDSVAVETDKYGFIPVNSKMAVLSSGEPVPNLWA
IGDATGKMMLAHAASAQGITVVENICGRDREPDYLSIPAAAFTHPEISYVGMTEPAAKDLGQKQGFEVASVRTYFKGNSK
AIAEDETDGIAKVIYRQDTGELLGVHIIGLHASDLIQEAANAIAKKQSVNELSFNVHTHPTLSEVLDEAFKRATVH
>Mature_475_residues
TQEFDYDLIIIGAGVGGHGAALHATSCGLKTAIVEVAEMGGTCVNRGCIPSKALLAASGKVRELRNAHHLKTLGIELDNV
SYDRQVMATHASNIVTKIRGDMSKSLKRLSVDIITGWAQVAGKQKVTVKTEKGEENFTAKDIILAPGSVPFVPPGIELDG
KTVFTSDDALKLDWLPPWVAIIGSGYIGLEFSDIYTALGSEITMIEALDKLMPTFDPDIAKIAQRVLIQSRDIEVKVGKL
AIKVVPGSPVIIELADAKTKEVEEIIEVDACLVATGRIPYTKDLGLDSVAVETDKYGFIPVNSKMAVLSSGEPVPNLWAI
GDATGKMMLAHAASAQGITVVENICGRDREPDYLSIPAAAFTHPEISYVGMTEPAAKDLGQKQGFEVASVRTYFKGNSKA
IAEDETDGIAKVIYRQDTGELLGVHIIGLHASDLIQEAANAIAKKQSVNELSFNVHTHPTLSEVLDEAFKRATVH

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=34.8837209302326, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI50301238, Length=474, Percent_Identity=28.4810126582279, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI291045266, Length=443, Percent_Identity=27.5395033860045, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI148277065, Length=489, Percent_Identity=27.4028629856851, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI148277071, Length=489, Percent_Identity=27.4028629856851, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519430, Length=489, Percent_Identity=27.4028629856851, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI33519428, Length=489, Percent_Identity=27.4028629856851, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI33519426, Length=489, Percent_Identity=27.4028629856851, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI22035672, Length=482, Percent_Identity=27.1784232365145, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI291045268, Length=441, Percent_Identity=25.1700680272109, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786307, Length=466, Percent_Identity=31.9742489270386, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082354, Length=471, Percent_Identity=27.8131634819533, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789915, Length=449, Percent_Identity=27.1714922048998, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87081717, Length=474, Percent_Identity=25.5274261603376, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=35.8208955223881, Blast_Score=254, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17557007, Length=483, Percent_Identity=26.2939958592132, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=474, Percent_Identity=27.0042194092827, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=474, Percent_Identity=27.0042194092827, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=505, Percent_Identity=25.7425742574257, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=481, Percent_Identity=32.016632016632, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6325240, Length=484, Percent_Identity=28.5123966942149, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=476, Percent_Identity=26.890756302521, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=37.4468085106383, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=28.4536082474227, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=28.4536082474227, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640551, Length=485, Percent_Identity=28.4536082474227, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17737741, Length=490, Percent_Identity=25.9183673469388, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50980; Mature: 50849

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEFDYDLIIIGAGVGGHGAALHATSCGLKTAIVEVAEMGGTCVNRGCIPSKALLAASG
CCCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHCCC
KVRELRNAHHLKTLGIELDNVSYDRQVMATHASNIVTKIRGDMSKSLKRLSVDIITGWAQ
CHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHH
VAGKQKVTVKTEKGEENFTAKDIILAPGSVPFVPPGIELDGKTVFTSDDALKLDWLPPWV
HCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCEECCCEEEECCCCEEEECCCHHH
AIIGSGYIGLEFSDIYTALGSEITMIEALDKLMPTFDPDIAKIAQRVLIQSRDIEVKVGK
HHHCCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEE
LAIKVVPGSPVIIELADAKTKEVEEIIEVDACLVATGRIPYTKDLGLDSVAVETDKYGFI
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCCEEEE
PVNSKMAVLSSGEPVPNLWAIGDATGKMMLAHAASAQGITVVENICGRDREPDYLSIPAA
ECCCCEEEEECCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCEEECCHH
AFTHPEISYVGMTEPAAKDLGQKQGFEVASVRTYFKGNSKAIAEDETDGIAKVIYRQDTG
HCCCCCEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCCCEECCCCCCCCEEEEEECCCC
ELLGVHIIGLHASDLIQEAANAIAKKQSVNELSFNVHTHPTLSEVLDEAFKRATVH
CEEEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQEFDYDLIIIGAGVGGHGAALHATSCGLKTAIVEVAEMGGTCVNRGCIPSKALLAASG
CCCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCEECCCCCCCHHHHHCCC
KVRELRNAHHLKTLGIELDNVSYDRQVMATHASNIVTKIRGDMSKSLKRLSVDIITGWAQ
CHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHH
VAGKQKVTVKTEKGEENFTAKDIILAPGSVPFVPPGIELDGKTVFTSDDALKLDWLPPWV
HCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCEECCCEEEECCCCEEEECCCHHH
AIIGSGYIGLEFSDIYTALGSEITMIEALDKLMPTFDPDIAKIAQRVLIQSRDIEVKVGK
HHHCCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEE
LAIKVVPGSPVIIELADAKTKEVEEIIEVDACLVATGRIPYTKDLGLDSVAVETDKYGFI
EEEEEECCCCEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCCEEEE
PVNSKMAVLSSGEPVPNLWAIGDATGKMMLAHAASAQGITVVENICGRDREPDYLSIPAA
ECCCCEEEEECCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCEEECCHH
AFTHPEISYVGMTEPAAKDLGQKQGFEVASVRTYFKGNSKAIAEDETDGIAKVIYRQDTG
HCCCCCEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCCCEECCCCCCCCEEEEEECCCC
ELLGVHIIGLHASDLIQEAANAIAKKQSVNELSFNVHTHPTLSEVLDEAFKRATVH
CEEEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]