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The map label for this gene is ytcB [H]

Identifier: 113476132

GI number: 113476132

Start: 3881068

End: 3882018

Strand: Direct

Name: ytcB [H]

Synonym: Tery_2514

Alternate gene names: 113476132

Gene position: 3881068-3882018 (Clockwise)

Preceding gene: 113476131

Following gene: 113476135

Centisome position: 50.08

GC content: 36.38

Gene sequence:

>951_bases
ATGAGTAATACTATAGTTACAGGAGTAGGTGGATTTATTGGATCTCATTTAGCAGAAACTCTATTAAATCAAGATAACAA
AGTAATAGGAATAGATGAGTTTAATAATTACTATAACCCTACTTTGAAGCGTCAGAATATTTCTCAATTTCAAGACCATC
CCGCTTTTCAATTAATAGAAAATGATATTCAGTCTTTAAATTGGTCAGAATTACTTGTAGATGTAGACATAATTTATCAT
CAAGCTGCTCAAGCTGGAGTTCGTGCTAGTTGGGGTGGAGGTTTTTTTGATTATACAGAACGTAATATAGATGCGACTCA
AATAATTTTAGAAGCTGCTAAAGATGCTCCTAATTTGAAAAGATTAGTTTATGCTTCTAGTTCTTCTGTATATGGTAATG
CTGAAACTTTCCCCACCCCAGAAACTGTTTGTCCTCAACCAGTATCTCCTTATGGAATTACTAAGTTAGCAGCAGAACAA
TTAGGCAAGTTATATCATCATAATTTTGGTGTGCCCTGCGTCTATTTACGCTACTTTACAGTATATGGTCCCCGGCAAAG
ACCTGACATGGCATTTCATAAATTTTTTAAGTGGATTTTACAAGATGAACCTATTTCTATTTATGGAGATGCTCAGCAAA
CTAGAGACTTTACCTTTATCAGCGATGCAGTCGCAGCTAACTTATTAGCCGCTACAGTACCATTAGCAGTAGGTGAGGTA
TTTAATATAGGTGGGGGTAGTCGGGTAGTATTAGCAGAAGTTATTAATATCATGGAGCAAATAGTAGGACGTCCAATTAA
AAAAAATTTTGTAGAAAAAGCTAGGGGAGATGCTCGTCATACTAGTGCCGATGTTTCTAAAGCACAGAAAATTTTGGGCT
ATCAACCCCAGATTTCTTTGGCCGAAGGTTTGAAGCGAGAGTGGGAGTGGATACGATCGCTTTATTCATAA

Upstream 100 bases:

>100_bases
TAGTCCTTGCTCAAATGTACTAAAAAAATTCTGTTAGTACCTAATTATAAGTTATGATCCATCTCAATGTGAATAAAAAT
AATAATATACCCAAACAAAA

Downstream 100 bases:

>100_bases
AAAAATTGCCAACTGCTACAACAAAAAGCAGGGCATCTCATCCTCGAACATAATCGAAAATTCCTTGCACAGATACTTTC
AGAGTTTGATAGGGAGAATA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSNTIVTGVGGFIGSHLAETLLNQDNKVIGIDEFNNYYNPTLKRQNISQFQDHPAFQLIENDIQSLNWSELLVDVDIIYH
QAAQAGVRASWGGGFFDYTERNIDATQIILEAAKDAPNLKRLVYASSSSVYGNAETFPTPETVCPQPVSPYGITKLAAEQ
LGKLYHHNFGVPCVYLRYFTVYGPRQRPDMAFHKFFKWILQDEPISIYGDAQQTRDFTFISDAVAANLLAATVPLAVGEV
FNIGGGSRVVLAEVINIMEQIVGRPIKKNFVEKARGDARHTSADVSKAQKILGYQPQISLAEGLKREWEWIRSLYS

Sequences:

>Translated_316_residues
MSNTIVTGVGGFIGSHLAETLLNQDNKVIGIDEFNNYYNPTLKRQNISQFQDHPAFQLIENDIQSLNWSELLVDVDIIYH
QAAQAGVRASWGGGFFDYTERNIDATQIILEAAKDAPNLKRLVYASSSSVYGNAETFPTPETVCPQPVSPYGITKLAAEQ
LGKLYHHNFGVPCVYLRYFTVYGPRQRPDMAFHKFFKWILQDEPISIYGDAQQTRDFTFISDAVAANLLAATVPLAVGEV
FNIGGGSRVVLAEVINIMEQIVGRPIKKNFVEKARGDARHTSADVSKAQKILGYQPQISLAEGLKREWEWIRSLYS
>Mature_315_residues
SNTIVTGVGGFIGSHLAETLLNQDNKVIGIDEFNNYYNPTLKRQNISQFQDHPAFQLIENDIQSLNWSELLVDVDIIYHQ
AAQAGVRASWGGGFFDYTERNIDATQIILEAAKDAPNLKRLVYASSSSVYGNAETFPTPETVCPQPVSPYGITKLAAEQL
GKLYHHNFGVPCVYLRYFTVYGPRQRPDMAFHKFFKWILQDEPISIYGDAQQTRDFTFISDAVAANLLAATVPLAVGEVF
NIGGGSRVVLAEVINIMEQIVGRPIKKNFVEKARGDARHTSADVSKAQKILGYQPQISLAEGLKREWEWIRSLYS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=319, Percent_Identity=30.0940438871473, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI42516563, Length=316, Percent_Identity=29.746835443038, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1788353, Length=341, Percent_Identity=26.099706744868, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786974, Length=333, Percent_Identity=27.9279279279279, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994969, Length=336, Percent_Identity=25.8928571428571, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790049, Length=321, Percent_Identity=24.9221183800623, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=27.8996865203762, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17568069, Length=329, Percent_Identity=27.0516717325228, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=338, Percent_Identity=27.810650887574, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=336, Percent_Identity=27.6785714285714, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=23.7082066869301, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=339, Percent_Identity=25.0737463126844, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356223, Length=316, Percent_Identity=28.4810126582279, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 35295; Mature: 35164

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTIVTGVGGFIGSHLAETLLNQDNKVIGIDEFNNYYNPTLKRQNISQFQDHPAFQLIE
CCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
NDIQSLNWSELLVDVDIIYHQAAQAGVRASWGGGFFDYTERNIDATQIILEAAKDAPNLK
HHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
RLVYASSSSVYGNAETFPTPETVCPQPVSPYGITKLAAEQLGKLYHHNFGVPCVYLRYFT
HHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VYGPRQRPDMAFHKFFKWILQDEPISIYGDAQQTRDFTFISDAVAANLLAATVPLAVGEV
HCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FNIGGGSRVVLAEVINIMEQIVGRPIKKNFVEKARGDARHTSADVSKAQKILGYQPQISL
HCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
AEGLKREWEWIRSLYS
HHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SNTIVTGVGGFIGSHLAETLLNQDNKVIGIDEFNNYYNPTLKRQNISQFQDHPAFQLIE
CCCEEEHHHHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
NDIQSLNWSELLVDVDIIYHQAAQAGVRASWGGGFFDYTERNIDATQIILEAAKDAPNLK
HHHHHCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
RLVYASSSSVYGNAETFPTPETVCPQPVSPYGITKLAAEQLGKLYHHNFGVPCVYLRYFT
HHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VYGPRQRPDMAFHKFFKWILQDEPISIYGDAQQTRDFTFISDAVAANLLAATVPLAVGEV
HCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FNIGGGSRVVLAEVINIMEQIVGRPIKKNFVEKARGDARHTSADVSKAQKILGYQPQISL
HCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
AEGLKREWEWIRSLYS
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]