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The map label for this gene is hsdM [H]

Identifier: 113476047

GI number: 113476047

Start: 3732458

End: 3733939

Strand: Direct

Name: hsdM [H]

Synonym: Tery_2418

Alternate gene names: 113476047

Gene position: 3732458-3733939 (Clockwise)

Preceding gene: 113476046

Following gene: 113476048

Centisome position: 48.16

GC content: 38.12

Gene sequence:

>1482_bases
ATGATTACAGGTGAACTAAAATCAAAAGTAGATAAGTTATGGTCGACGTTCTGGAGCAATGGTATTAGTAACCCATTGTC
AGTTATTGAACAAATATCTTACCTATTATTTATTAAACAACTTGATCAGCAGGAAGCAGTTAAGGAGAAAAAAGCGCGAC
GGCTAAAAAAGGTAAAAGAAAAACTAATATTTTCAGAAGCATCACAAAGCTGTCGATGGTCTCACTTCAAGGAATTAGCA
ACAGATAAGATGTTTGAGGCTGTAAGGGATGAGGCTTTTCCTTTTATCAAAACTTTGGGGGGAACTTTAGAAAATAATGC
CTACTCTCGACACATGAAGGATGCTGTATTTATGATAGGCAGCCCGGCATTATTGGCAAATGTGGTGCAACAAATAGATA
GTTTACCCCTCGATGACCGAGATACTAAGGGGGACCTCTATGAATATATGCTTTCTAAGCTGAATACTGCGGGGCAAAAT
GGACAGTTTAGGACACCGCGCCACATTATTAAGATGATAGTGGACTTGATGACACCCCAACCTAATGATGTCGTTTGTGA
CCCTGCTTTTGGTACGGCTGGGTTTTTGGTCGCCGTAGCAGAACATTTACAACAACTCAAGGATGAGAATGGTTCCTTAG
TTTTGAATGCACCAGGAAACAAAGAGCATTTTTATCAGCATATGTTTCACGGTTTTGACTTTGATGCCACAATGTTGCGT
ATTGGTAGTATGAATTTAATGCAGCATGGTATTGAAAATGCTCAAATTGAAGCGAGGGATGCTTTATCGGAAGACCACGC
GGGGGTTGAGGAAATGTTTACTTTGGTTTTGGCTAACCCACCGTTTAAGGGGTCGATACAAAAGTCAAGTATTGCTAAAG
ATTTAACTAAAATTGTTAATACTACTAAAACAGAGTTGTTATTTTTGGCTTTATTTTTAAGGTTATTAAAAACGGGGGGT
CGGGCGGCGGTTATTGTGCCTGATGGGGTTTTATTTGGTTCTTCTAAAGCCCATAAAGATGTTCGTAAGATGTTAGTTGA
AGAGCATAAGTTAGATGGAGTTATTTCTATGCCATCGGGGGTTTTTAAACCTTATGCGGGGGTATCAACAGCGATTTTAA
TGTTTACAAAAACGGGGGCGGGTGGTACTGATTTTGTTTGGTTTTGTGATATGCAGGCGGATGGTTTTTCGTTGGATGAT
AAACGGCAACCTGTAGAAGAAAATGACATTTCTAATATTACTAAAAGTTGGCAAAAACGGAATCCTAAAAAAGATAAGGA
CCGGAATAGTAAGACGTTTTTTATACCGAAGGATGAGATAAAAGATAATGGTTATGATCTTTCTATTAATAGATATAAAG
AGGTGGCATACGAGGAGATAGAATATGAGCATCCTTTAGTAATTTTAGGTAAGTTACGAGAATTAGAGGATGAAATTAAT
CAAGATTTGGATGAGTTGGGGCAGTTATTAAATGGAGATTAA

Upstream 100 bases:

>100_bases
TTATACTAAAATTAGTGTCTTTGGGTAAGCTCTGTTAGAGTTATAATTGTCAAAAGTTTTCTTTTCTTAAGGCTAGGAAT
TAATAAACTAACAAAACTAA

Downstream 100 bases:

>100_bases
TCTGCAAAAGGAGGAGTTTAGTTATGCTTATATTTGAGGGGTTGCTACTGTGGCGGGTTGTGTTTTTCAAGGAACTACTC
CCCCTTTAGATGGGTTGGGA

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MITGELKSKVDKLWSTFWSNGISNPLSVIEQISYLLFIKQLDQQEAVKEKKARRLKKVKEKLIFSEASQSCRWSHFKELA
TDKMFEAVRDEAFPFIKTLGGTLENNAYSRHMKDAVFMIGSPALLANVVQQIDSLPLDDRDTKGDLYEYMLSKLNTAGQN
GQFRTPRHIIKMIVDLMTPQPNDVVCDPAFGTAGFLVAVAEHLQQLKDENGSLVLNAPGNKEHFYQHMFHGFDFDATMLR
IGSMNLMQHGIENAQIEARDALSEDHAGVEEMFTLVLANPPFKGSIQKSSIAKDLTKIVNTTKTELLFLALFLRLLKTGG
RAAVIVPDGVLFGSSKAHKDVRKMLVEEHKLDGVISMPSGVFKPYAGVSTAILMFTKTGAGGTDFVWFCDMQADGFSLDD
KRQPVEENDISNITKSWQKRNPKKDKDRNSKTFFIPKDEIKDNGYDLSINRYKEVAYEEIEYEHPLVILGKLRELEDEIN
QDLDELGQLLNGD

Sequences:

>Translated_493_residues
MITGELKSKVDKLWSTFWSNGISNPLSVIEQISYLLFIKQLDQQEAVKEKKARRLKKVKEKLIFSEASQSCRWSHFKELA
TDKMFEAVRDEAFPFIKTLGGTLENNAYSRHMKDAVFMIGSPALLANVVQQIDSLPLDDRDTKGDLYEYMLSKLNTAGQN
GQFRTPRHIIKMIVDLMTPQPNDVVCDPAFGTAGFLVAVAEHLQQLKDENGSLVLNAPGNKEHFYQHMFHGFDFDATMLR
IGSMNLMQHGIENAQIEARDALSEDHAGVEEMFTLVLANPPFKGSIQKSSIAKDLTKIVNTTKTELLFLALFLRLLKTGG
RAAVIVPDGVLFGSSKAHKDVRKMLVEEHKLDGVISMPSGVFKPYAGVSTAILMFTKTGAGGTDFVWFCDMQADGFSLDD
KRQPVEENDISNITKSWQKRNPKKDKDRNSKTFFIPKDEIKDNGYDLSINRYKEVAYEEIEYEHPLVILGKLRELEDEIN
QDLDELGQLLNGD
>Mature_493_residues
MITGELKSKVDKLWSTFWSNGISNPLSVIEQISYLLFIKQLDQQEAVKEKKARRLKKVKEKLIFSEASQSCRWSHFKELA
TDKMFEAVRDEAFPFIKTLGGTLENNAYSRHMKDAVFMIGSPALLANVVQQIDSLPLDDRDTKGDLYEYMLSKLNTAGQN
GQFRTPRHIIKMIVDLMTPQPNDVVCDPAFGTAGFLVAVAEHLQQLKDENGSLVLNAPGNKEHFYQHMFHGFDFDATMLR
IGSMNLMQHGIENAQIEARDALSEDHAGVEEMFTLVLANPPFKGSIQKSSIAKDLTKIVNTTKTELLFLALFLRLLKTGG
RAAVIVPDGVLFGSSKAHKDVRKMLVEEHKLDGVISMPSGVFKPYAGVSTAILMFTKTGAGGTDFVWFCDMQADGFSLDD
KRQPVEENDISNITKSWQKRNPKKDKDRNSKTFFIPKDEIKDNGYDLSINRYKEVAYEEIEYEHPLVILGKLRELEDEIN
QDLDELGQLLNGD

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=308, Percent_Identity=33.7662337662338, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55609; Mature: 55609

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]