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The map label for this gene is budB [H]

Identifier: 113475595

GI number: 113475595

Start: 2987968

End: 2989605

Strand: Direct

Name: budB [H]

Synonym: Tery_1928

Alternate gene names: 113475595

Gene position: 2987968-2989605 (Clockwise)

Preceding gene: 113475593

Following gene: 113475600

Centisome position: 38.55

GC content: 39.01

Gene sequence:

>1638_bases
ATGAACACTGCAGAATTATTAATCAAATGCCTCGAAAACGAAGGGGTAAAATATATATTTGGACTTCCTGGAGAAGAGAA
TTTGAACATTTTAGAAGCTCTGAAAAATTCTTCAATCCAATTTATTACTACCCGACATGAACAAGGTGCTGCTTTTATGG
CAGATGTCTACGGTCGTCTAACAGGAAAAGCAGGAGTTTGTTTATCAACATTAGGTCCTGGTGCTACTAATTTAATGACT
GGTGTTGCTGATGCTAATTTGGACGGCGCACCTTTAGTAGCAATTACAGGTCAAGTTGGTACTGATAGAATGCACATAGA
ATCTCACCAATATTTAGATTTGGTGGCTATGTTTTCTCCAGTAACTAAATGGAATGCTCAAATTCTTCGCCCTAGTAATA
CACCAGAAATTGTTCGCAAAGCATTTAAAGTAGCTCAACAAGAAAAACCTGGTGCGATTCATATCGACCTACCAGAAAAC
ATTGCAGCGATGTCTGTAGAAGGGAAACCCTTGAAGCAGGACAAGCGGGAAAAATCTTATTCTGCTTATCAGAGTATGAC
CGAAGCAGCAGTGGCAATTTCTAAAGCCAAAAACCCGATGATTTTGGTAGGAAATGGAGCTCTTCGAGGGAATGCAAGTG
AAATTGTAACGGAGTTTGCTACACGATTAAATATTCCTGTTGCTAATACTTTTATGGGTAAAGGGGTGATTCCTTATACT
CATCCTCTGTCTTTATGGACAACAGGATTACAACAACGGGATTTTATTACTTGTGCTTTTGAGCAAACAGATTTGGTGAT
TGCAGTTGGTTACGACTTGATTGAATATTCACCAAAAAAATGGAATCCTCAAGGTAAAATTCCAGTTATTCATGTTGGGG
CAAAATATGCAGAGATTGACAGTAGTTATATTCCTATCGCTGAAATAGTAGGAGATATTTCTGATTCTTTGAATGAAATT
TTGCGACGCTCCGAGCGCATAAATCAATTAAGCCCAACTGTATCAGAGATTAGAAAAAGTATGCGCTATGAATATGAGGG
ACATGGCAAGGATGATGGGTTTCCAATTAAGCCTCAAAAACTAATTTATGATTTACGTCAGGTTATGGGTCCGGAGGATA
TTGTCATCTCTGATGTTGGTGCCCATAAAATGTGGATTGCTAGGCATTATCATTGTGAACGTCCTAATACTTGTTTGATT
TCTAACGGATTTGCGGCGATGGGTATTGCGATTCCAGGAGCGATCGCGGCGAAGTTAGTTTATCCTGAGCGTAAGGTTGT
GGCAGCTACGGGGGATGGTGGGTTTATGATGAATTGTCAAGAGTTGGAAACTGCTTTACGAGTGGGTACTAATTTTGTGA
CTTTGATTTTTAATGATGGTGGTTATGGTTTGATTGAGTGGAAACAGGAAAACCATTTTGGGGAGTCATCTTTTATTAAG
TTCGGCAATCCAGATTTTGTTAAGTTTGCTGAAAGTTTTGGTTTGAAAGGTTATCGAGTTGAGTCTGCTGCTGATTTAAT
TCCTACTCTGAAAGAGGCTCTTGCTCAAGATGTTCCTGCTGTGATAGACTGCCCTATTGATTACCGAGAAAATTCTCGTT
TTAGTCAAAAATCAGGTGGTTTAAATTGTTCTGTTTAG

Upstream 100 bases:

>100_bases
TTCAGATGTTTAAACTGCAATTAGTGTTTTTGCGTAAGTCCTGATTTTTATAATGTTGATTAAGTTTTTAAATACCTTTT
ATGAGAGGAGAACAATAAAA

Downstream 100 bases:

>100_bases
GCTTTGCTGAAAAACTTTTTGGTGAAGGGAAGCAATTGTTGACTTGACAAAAGCTTTTTGAGGTTTAACAGCAGTAAAAT
TTTTACGAGTTCTAGTTTCC

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNTAELLIKCLENEGVKYIFGLPGEENLNILEALKNSSIQFITTRHEQGAAFMADVYGRLTGKAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFSPVTKWNAQILRPSNTPEIVRKAFKVAQQEKPGAIHIDLPEN
IAAMSVEGKPLKQDKREKSYSAYQSMTEAAVAISKAKNPMILVGNGALRGNASEIVTEFATRLNIPVANTFMGKGVIPYT
HPLSLWTTGLQQRDFITCAFEQTDLVIAVGYDLIEYSPKKWNPQGKIPVIHVGAKYAEIDSSYIPIAEIVGDISDSLNEI
LRRSERINQLSPTVSEIRKSMRYEYEGHGKDDGFPIKPQKLIYDLRQVMGPEDIVISDVGAHKMWIARHYHCERPNTCLI
SNGFAAMGIAIPGAIAAKLVYPERKVVAATGDGGFMMNCQELETALRVGTNFVTLIFNDGGYGLIEWKQENHFGESSFIK
FGNPDFVKFAESFGLKGYRVESAADLIPTLKEALAQDVPAVIDCPIDYRENSRFSQKSGGLNCSV

Sequences:

>Translated_545_residues
MNTAELLIKCLENEGVKYIFGLPGEENLNILEALKNSSIQFITTRHEQGAAFMADVYGRLTGKAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFSPVTKWNAQILRPSNTPEIVRKAFKVAQQEKPGAIHIDLPEN
IAAMSVEGKPLKQDKREKSYSAYQSMTEAAVAISKAKNPMILVGNGALRGNASEIVTEFATRLNIPVANTFMGKGVIPYT
HPLSLWTTGLQQRDFITCAFEQTDLVIAVGYDLIEYSPKKWNPQGKIPVIHVGAKYAEIDSSYIPIAEIVGDISDSLNEI
LRRSERINQLSPTVSEIRKSMRYEYEGHGKDDGFPIKPQKLIYDLRQVMGPEDIVISDVGAHKMWIARHYHCERPNTCLI
SNGFAAMGIAIPGAIAAKLVYPERKVVAATGDGGFMMNCQELETALRVGTNFVTLIFNDGGYGLIEWKQENHFGESSFIK
FGNPDFVKFAESFGLKGYRVESAADLIPTLKEALAQDVPAVIDCPIDYRENSRFSQKSGGLNCSV
>Mature_545_residues
MNTAELLIKCLENEGVKYIFGLPGEENLNILEALKNSSIQFITTRHEQGAAFMADVYGRLTGKAGVCLSTLGPGATNLMT
GVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFSPVTKWNAQILRPSNTPEIVRKAFKVAQQEKPGAIHIDLPEN
IAAMSVEGKPLKQDKREKSYSAYQSMTEAAVAISKAKNPMILVGNGALRGNASEIVTEFATRLNIPVANTFMGKGVIPYT
HPLSLWTTGLQQRDFITCAFEQTDLVIAVGYDLIEYSPKKWNPQGKIPVIHVGAKYAEIDSSYIPIAEIVGDISDSLNEI
LRRSERINQLSPTVSEIRKSMRYEYEGHGKDDGFPIKPQKLIYDLRQVMGPEDIVISDVGAHKMWIARHYHCERPNTCLI
SNGFAAMGIAIPGAIAAKLVYPERKVVAATGDGGFMMNCQELETALRVGTNFVTLIFNDGGYGLIEWKQENHFGESSFIK
FGNPDFVKFAESFGLKGYRVESAADLIPTLKEALAQDVPAVIDCPIDYRENSRFSQKSGGLNCSV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=25.3597122302158, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI21361361, Length=579, Percent_Identity=23.8341968911917, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1790104, Length=535, Percent_Identity=32.1495327102804, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI87081685, Length=564, Percent_Identity=30.3191489361702, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1786717, Length=563, Percent_Identity=26.2877442273535, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=23.8007380073801, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788716, Length=544, Percent_Identity=26.2867647058824, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=556, Percent_Identity=25.3597122302158, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=556, Percent_Identity=25.3597122302158, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=586, Percent_Identity=24.4027303754266, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=31.2387791741472, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6320816, Length=535, Percent_Identity=26.3551401869159, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6321524, Length=523, Percent_Identity=22.5621414913958, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=554, Percent_Identity=22.0216606498195, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=521, Percent_Identity=21.4971209213052, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922626, Length=559, Percent_Identity=25.5813953488372, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59772; Mature: 59772

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAELLIKCLENEGVKYIFGLPGEENLNILEALKNSSIQFITTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
TGKAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFSP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHCC
VTKWNAQILRPSNTPEIVRKAFKVAQQEKPGAIHIDLPENIAAMSVEGKPLKQDKREKSY
CCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHH
SAYQSMTEAAVAISKAKNPMILVGNGALRGNASEIVTEFATRLNIPVANTFMGKGVIPYT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
HPLSLWTTGLQQRDFITCAFEQTDLVIAVGYDLIEYSPKKWNPQGKIPVIHVGAKYAEID
CCHHHHHCCCCCCCEEEEEECCCCEEEEECCHHHHCCCCCCCCCCCEEEEEECCCCEECC
SSYIPIAEIVGDISDSLNEILRRSERINQLSPTVSEIRKSMRYEYEGHGKDDGFPIKPQK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LIYDLRQVMGPEDIVISDVGAHKMWIARHYHCERPNTCLISNGFAAMGIAIPGAIAAKLV
HHHHHHHHCCCCCEEEECCCCCHHHHHHHEECCCCCEEEEECCCEEEEEECCCHHHHEEE
YPERKVVAATGDGGFMMNCQELETALRVGTNFVTLIFNDGGYGLIEWKQENHFGESSFIK
CCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEE
FGNPDFVKFAESFGLKGYRVESAADLIPTLKEALAQDVPAVIDCPIDYRENSRFSQKSGG
CCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHCCC
LNCSV
CCCCC
>Mature Secondary Structure
MNTAELLIKCLENEGVKYIFGLPGEENLNILEALKNSSIQFITTRHEQGAAFMADVYGRL
CCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
TGKAGVCLSTLGPGATNLMTGVADANLDGAPLVAITGQVGTDRMHIESHQYLDLVAMFSP
CCCCCEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCEEEECCHHHHHHHHHHCC
VTKWNAQILRPSNTPEIVRKAFKVAQQEKPGAIHIDLPENIAAMSVEGKPLKQDKREKSY
CCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHH
SAYQSMTEAAVAISKAKNPMILVGNGALRGNASEIVTEFATRLNIPVANTFMGKGVIPYT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
HPLSLWTTGLQQRDFITCAFEQTDLVIAVGYDLIEYSPKKWNPQGKIPVIHVGAKYAEID
CCHHHHHCCCCCCCEEEEEECCCCEEEEECCHHHHCCCCCCCCCCCEEEEEECCCCEECC
SSYIPIAEIVGDISDSLNEILRRSERINQLSPTVSEIRKSMRYEYEGHGKDDGFPIKPQK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LIYDLRQVMGPEDIVISDVGAHKMWIARHYHCERPNTCLISNGFAAMGIAIPGAIAAKLV
HHHHHHHHCCCCCEEEECCCCCHHHHHHHEECCCCCEEEEECCCEEEEEECCCHHHHEEE
YPERKVVAATGDGGFMMNCQELETALRVGTNFVTLIFNDGGYGLIEWKQENHFGESSFIK
CCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEE
FGNPDFVKFAESFGLKGYRVESAADLIPTLKEALAQDVPAVIDCPIDYRENSRFSQKSGG
CCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHCCC
LNCSV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644303 [H]