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The map label for this gene is rfaI [H]

Identifier: 113474875

GI number: 113474875

Start: 1726892

End: 1727728

Strand: Reverse

Name: rfaI [H]

Synonym: Tery_1098

Alternate gene names: 113474875

Gene position: 1727728-1726892 (Counterclockwise)

Preceding gene: 113474877

Following gene: 113474871

Centisome position: 22.29

GC content: 27.0

Gene sequence:

>837_bases
ATGATGAATCTATTATTTTGTTTTGATCAAAACTATCAACAACACTTCGGTGTAGCAATCACTTCTGTATTATTAAATAA
TTTATCTAGTCATTTTGATGTACATATAATCACAAATTTTATGGAAGAAAAACTAAAACAAAAATTAGATACGCTGAGTA
AAAATTATAAATGTTCATTTCATTTATATATAATCAATAACCTTGATAAAATTAGCAAATTAAAAGTATCTGATCATGTT
TCCAATGCAACTTATTATAGATTAATAATGGCTGAAATTCTCCCTAAGCATATAGATAAAGTTCTTTATTTAGATTCTGA
TGTTGTTGTAATTTCTCCCTTAGAAGAACTATATAATATTGATCTAGAAAATTACTTTATTGCTGCTTCAGGATTTTCTG
GCACTTTGGTAAAAAGTAAAGGTTTTAATAGTGGTGTTATGGTTGTTAACTTAGAAAAATGGAGAAATGAACAAATTTCA
ACAAAAGTAATTGATTTTGCAACTAAAAATAGAGATAAGCTGCCATACCACGACCAAAGCGCTTTAAATCGTGTAATCAA
ACAGAATTACTTAATAATTGATCGGAAATGGAATTTTCAAGTGGACTTAAGCCCAAGAAAAATTCAAAAACCTGATGACA
ATATCGCTTTGAAAAATGCTAGAATTATACATTATATTGGTTCTTCAAAACCTTGGTATTTTTGGATATCTGATCAACGG
AAAAATATATATGAATTATATTTAAAAAAATCATTGTGGTCTACATCCAAATTACAGATGATTTTCCAGCAAACTGTATA
TTTTCGAAAAGCCTTACAGAGAAAACTTAAGAAATAA

Upstream 100 bases:

>100_bases
TATTGTTCCTTCTGCCTCCTTGCTCCTACCTTAAAACCGCCTGAATATTTTATTATTATGATAAGATGATTATAGAAATA
TCAGGGCATAAAAGAGAAGA

Downstream 100 bases:

>100_bases
GTTTTAATTCATCTTTACAAGTAAGCTGAAAAAAACTGTTATCTAAAATCAGGTTATACAATTCTAGTATTTGCTTAAAA
CCATAAAGATCGACCTTATG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MMNLLFCFDQNYQQHFGVAITSVLLNNLSSHFDVHIITNFMEEKLKQKLDTLSKNYKCSFHLYIINNLDKISKLKVSDHV
SNATYYRLIMAEILPKHIDKVLYLDSDVVVISPLEELYNIDLENYFIAASGFSGTLVKSKGFNSGVMVVNLEKWRNEQIS
TKVIDFATKNRDKLPYHDQSALNRVIKQNYLIIDRKWNFQVDLSPRKIQKPDDNIALKNARIIHYIGSSKPWYFWISDQR
KNIYELYLKKSLWSTSKLQMIFQQTVYFRKALQRKLKK

Sequences:

>Translated_278_residues
MMNLLFCFDQNYQQHFGVAITSVLLNNLSSHFDVHIITNFMEEKLKQKLDTLSKNYKCSFHLYIINNLDKISKLKVSDHV
SNATYYRLIMAEILPKHIDKVLYLDSDVVVISPLEELYNIDLENYFIAASGFSGTLVKSKGFNSGVMVVNLEKWRNEQIS
TKVIDFATKNRDKLPYHDQSALNRVIKQNYLIIDRKWNFQVDLSPRKIQKPDDNIALKNARIIHYIGSSKPWYFWISDQR
KNIYELYLKKSLWSTSKLQMIFQQTVYFRKALQRKLKK
>Mature_278_residues
MMNLLFCFDQNYQQHFGVAITSVLLNNLSSHFDVHIITNFMEEKLKQKLDTLSKNYKCSFHLYIINNLDKISKLKVSDHV
SNATYYRLIMAEILPKHIDKVLYLDSDVVVISPLEELYNIDLENYFIAASGFSGTLVKSKGFNSGVMVVNLEKWRNEQIS
TKVIDFATKNRDKLPYHDQSALNRVIKQNYLIIDRKWNFQVDLSPRKIQKPDDNIALKNARIIHYIGSSKPWYFWISDQR
KNIYELYLKKSLWSTSKLQMIFQQTVYFRKALQRKLKK

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=253, Percent_Identity=26.8774703557312, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790056, Length=267, Percent_Identity=28.0898876404494, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 32835; Mature: 32835

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNLLFCFDQNYQQHFGVAITSVLLNNLSSHFDVHIITNFMEEKLKQKLDTLSKNYKCSF
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
HLYIINNLDKISKLKVSDHVSNATYYRLIMAEILPKHIDKVLYLDSDVVVISPLEELYNI
EEEEECCCCHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECHHHHHCC
DLENYFIAASGFSGTLVKSKGFNSGVMVVNLEKWRNEQISTKVIDFATKNRDKLPYHDQS
CCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHCCCCHHHHHEEHHCCCCCCCCCCCHH
ALNRVIKQNYLIIDRKWNFQVDLSPRKIQKPDDNIALKNARIIHYIGSSKPWYFWISDQR
HHHHHHHCCEEEEEECCCEEEECCHHHCCCCCCCEEEECCEEEEEECCCCCEEEEEECCC
KNIYELYLKKSLWSTSKLQMIFQQTVYFRKALQRKLKK
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMNLLFCFDQNYQQHFGVAITSVLLNNLSSHFDVHIITNFMEEKLKQKLDTLSKNYKCSF
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
HLYIINNLDKISKLKVSDHVSNATYYRLIMAEILPKHIDKVLYLDSDVVVISPLEELYNI
EEEEECCCCHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCEEEEECHHHHHCC
DLENYFIAASGFSGTLVKSKGFNSGVMVVNLEKWRNEQISTKVIDFATKNRDKLPYHDQS
CCCCEEEEECCCCCEEEECCCCCCCEEEEEEHHHCCCCHHHHHEEHHCCCCCCCCCCCHH
ALNRVIKQNYLIIDRKWNFQVDLSPRKIQKPDDNIALKNARIIHYIGSSKPWYFWISDQR
HHHHHHHCCEEEEEECCCEEEECCHHHCCCCCCCEEEECCEEEEEECCCCCEEEEEECCC
KNIYELYLKKSLWSTSKLQMIFQQTVYFRKALQRKLKK
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]