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The map label for this gene is nirA [H]

Identifier: 113474849

GI number: 113474849

Start: 1672964

End: 1674496

Strand: Direct

Name: nirA [H]

Synonym: Tery_1068

Alternate gene names: 113474849

Gene position: 1672964-1674496 (Clockwise)

Preceding gene: 113474847

Following gene: 113474850

Centisome position: 21.59

GC content: 40.44

Gene sequence:

>1533_bases
ATGACTGAGACTATACAGCGCAAAATGAAACTAAATAGTTTTGATAAATTTAAGGCAGAAAAAGATGGTCTAGCAGTAAA
AACAGAAATAGAACATTTTGCTAAAATAGGTTGGGAAGCAATAGACAAAACAGACCGCAACCACAGACTTAAATGGTTGG
GTGTATTCTTTAGACCTGTAACTCCAGGCAAATTTATGTTACGGATGCGTATGCCTAATGGAATGCTCTCAAGTCAACAA
ATGCGAGTCTTGGCAGGAATAGTCCAACGCTACGGCGATGATGGCAATGCCGACATTACCACCCGACAAAATTTACAACT
GCGAGGAATTGCAATTGAAGACATTCCAGACATTTTCAATAGACTTGAAAAAGTTGGCTTAACCAGTGTTCAATCTGGCA
TGGATAATGTGCGAAATATCACAGGTTCCCCAGTTGCTGGCATTGATGCTACCGAACTATACGATACCCGTGAGTTAATC
AAGCAAGTCCAAGACATGATTACTGACAACAATAAAGGCAACTTTGAGTTTACTAATTTACCAAGAAAATTCAATATAGC
GATCGCCGGATGTCGTGATAATTCAGTCCACGCCGAAATCAACGATATTGCATTTATTCCAGCCTACAAAAATGATAAAT
TTGGCTTCAACGTTCTGGTAGGTGGCTTCTTTTCCGCCAAACGGTGCGAAGCCGCTATACCTCTAAATGCTTGGGTAGAA
CCCAATCAAGTCATTGCCCTTTGTCGAGCTATCTTAGAAGTATATCGTGATTACGGACTCCGGGAAAATCGGCAAAAATC
AAGGTTAATGTGGCTTATTGACGAGTGGGGGCTCACAAAGTTCCGCTCACAAGTTGAGAAACAAATAGGGCTAACTCTAG
AAACAGAAGCCCTCAAAGATGAAATTCTCTGGGAAAAGCGTGACCACATTGGCATTTACAAACAAAAACAACCAGGGTTA
AACTACATTGGGTTAAATATTCCTGTTGGCAGATTATATGCTGTCGATATGTTTGACCTAGCTCGTTTGGCTGAAGTTTA
TGGCAATGGAGAAATCCGTTTGACGGTAGAACAAAATGTCATCATTCCTAATGTTCCTGATTCTCGGCTGGAAACATTTA
GAAGTGAACCCCTCCTGAAAAAATTTTCTTTTGAGCCTCAACCACTAACTAGAGCTGTAGTTTCTTGTACAGGATCACAA
TTTTGTAACTTTGCCTTGATAGAAACTAAAAATCGCGCCTTAGCAATAGCAAAAGAACTAGAAACGGAACTATCAATACC
AAAACCAGTACGCATTCATTGGACTGGTTGCCCCAACTCTTGCGGACAACCACAGGTTGCAGACATTGGTCTGATGGGCA
CTAAAGTTCGCAAAAACGGGAAAATGGTCGATGGTGTTAATCTATATATGGGTGGGAAAGTTGGTAAAGATGCTCAACTT
GGCAGTTGTGTTCAAAAAAGTATTCCTTGTGAAGACCTCAAGCCAATATTGCGGAGTTTGCTAATTAAAAATTTTGCTGC
TCAACCAAAATAA

Upstream 100 bases:

>100_bases
TAATCTTTTAAAAAAGTTTTTTCTTCTAGTAAAGATCTTAAAAAAACAAACAATTCACTAAAAATTAGACATAGAAAATA
TATAAATCAAGGAAAAGTAG

Downstream 100 bases:

>100_bases
GTCTGTGCTCAAAGTTATTGAACGAGTACACAGAGAGTTATTCAATTCTTACCCAGTAGTACCATCCTGCTTGATGTAAA
GGATAGGTTATGGCTAAGGC

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTETIQRKMKLNSFDKFKAEKDGLAVKTEIEHFAKIGWEAIDKTDRNHRLKWLGVFFRPVTPGKFMLRMRMPNGMLSSQQ
MRVLAGIVQRYGDDGNADITTRQNLQLRGIAIEDIPDIFNRLEKVGLTSVQSGMDNVRNITGSPVAGIDATELYDTRELI
KQVQDMITDNNKGNFEFTNLPRKFNIAIAGCRDNSVHAEINDIAFIPAYKNDKFGFNVLVGGFFSAKRCEAAIPLNAWVE
PNQVIALCRAILEVYRDYGLRENRQKSRLMWLIDEWGLTKFRSQVEKQIGLTLETEALKDEILWEKRDHIGIYKQKQPGL
NYIGLNIPVGRLYAVDMFDLARLAEVYGNGEIRLTVEQNVIIPNVPDSRLETFRSEPLLKKFSFEPQPLTRAVVSCTGSQ
FCNFALIETKNRALAIAKELETELSIPKPVRIHWTGCPNSCGQPQVADIGLMGTKVRKNGKMVDGVNLYMGGKVGKDAQL
GSCVQKSIPCEDLKPILRSLLIKNFAAQPK

Sequences:

>Translated_510_residues
MTETIQRKMKLNSFDKFKAEKDGLAVKTEIEHFAKIGWEAIDKTDRNHRLKWLGVFFRPVTPGKFMLRMRMPNGMLSSQQ
MRVLAGIVQRYGDDGNADITTRQNLQLRGIAIEDIPDIFNRLEKVGLTSVQSGMDNVRNITGSPVAGIDATELYDTRELI
KQVQDMITDNNKGNFEFTNLPRKFNIAIAGCRDNSVHAEINDIAFIPAYKNDKFGFNVLVGGFFSAKRCEAAIPLNAWVE
PNQVIALCRAILEVYRDYGLRENRQKSRLMWLIDEWGLTKFRSQVEKQIGLTLETEALKDEILWEKRDHIGIYKQKQPGL
NYIGLNIPVGRLYAVDMFDLARLAEVYGNGEIRLTVEQNVIIPNVPDSRLETFRSEPLLKKFSFEPQPLTRAVVSCTGSQ
FCNFALIETKNRALAIAKELETELSIPKPVRIHWTGCPNSCGQPQVADIGLMGTKVRKNGKMVDGVNLYMGGKVGKDAQL
GSCVQKSIPCEDLKPILRSLLIKNFAAQPK
>Mature_509_residues
TETIQRKMKLNSFDKFKAEKDGLAVKTEIEHFAKIGWEAIDKTDRNHRLKWLGVFFRPVTPGKFMLRMRMPNGMLSSQQM
RVLAGIVQRYGDDGNADITTRQNLQLRGIAIEDIPDIFNRLEKVGLTSVQSGMDNVRNITGSPVAGIDATELYDTRELIK
QVQDMITDNNKGNFEFTNLPRKFNIAIAGCRDNSVHAEINDIAFIPAYKNDKFGFNVLVGGFFSAKRCEAAIPLNAWVEP
NQVIALCRAILEVYRDYGLRENRQKSRLMWLIDEWGLTKFRSQVEKQIGLTLETEALKDEILWEKRDHIGIYKQKQPGLN
YIGLNIPVGRLYAVDMFDLARLAEVYGNGEIRLTVEQNVIIPNVPDSRLETFRSEPLLKKFSFEPQPLTRAVVSCTGSQF
CNFALIETKNRALAIAKELETELSIPKPVRIHWTGCPNSCGQPQVADIGLMGTKVRKNGKMVDGVNLYMGGKVGKDAQLG
SCVQKSIPCEDLKPILRSLLIKNFAAQPK

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=450, Percent_Identity=23.5555555555556, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322597, Length=484, Percent_Identity=25.2066115702479, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 57585; Mature: 57454

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETIQRKMKLNSFDKFKAEKDGLAVKTEIEHFAKIGWEAIDKTDRNHRLKWLGVFFRPV
CCHHHHHHHHCCCHHHHCCCCCCCEEEHHHHHHHHHCHHHHCCCCCCCEEEEEEEEEECC
TPGKFMLRMRMPNGMLSSQQMRVLAGIVQRYGDDGNADITTRQNLQLRGIAIEDIPDIFN
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEHHHHHHHHH
RLEKVGLTSVQSGMDNVRNITGSPVAGIDATELYDTRELIKQVQDMITDNNKGNFEFTNL
HHHHHCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
PRKFNIAIAGCRDNSVHAEINDIAFIPAYKNDKFGFNVLVGGFFSAKRCEAAIPLNAWVE
CCEEEEEEEECCCCCCEEEECCEEEEECCCCCCCCEEEEEECCCCHHHCCEECCCCCCCC
PNQVIALCRAILEVYRDYGLRENRQKSRLMWLIDEWGLTKFRSQVEKQIGLTLETEALKD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEHHHHHH
EILWEKRDHIGIYKQKQPGLNYIGLNIPVGRLYAVDMFDLARLAEVYGNGEIRLTVEQNV
HHHHHCCCCCCEEECCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCCEEEEEEECCE
IIPNVPDSRLETFRSEPLLKKFSFEPQPLTRAVVSCTGSQFCNFALIETKNRALAIAKEL
EECCCCHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCHHCCEEEEECCCCHHHHHHHH
ETELSIPKPVRIHWTGCPNSCGQPQVADIGLMGTKVRKNGKMVDGVNLYMGGKVGKDAQL
HHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEECCCCCEECCEEEEECCCCCCCCHH
GSCVQKSIPCEDLKPILRSLLIKNFAAQPK
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TETIQRKMKLNSFDKFKAEKDGLAVKTEIEHFAKIGWEAIDKTDRNHRLKWLGVFFRPV
CHHHHHHHHCCCHHHHCCCCCCCEEEHHHHHHHHHCHHHHCCCCCCCEEEEEEEEEECC
TPGKFMLRMRMPNGMLSSQQMRVLAGIVQRYGDDGNADITTRQNLQLRGIAIEDIPDIFN
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEHHHHHHHHH
RLEKVGLTSVQSGMDNVRNITGSPVAGIDATELYDTRELIKQVQDMITDNNKGNFEFTNL
HHHHHCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
PRKFNIAIAGCRDNSVHAEINDIAFIPAYKNDKFGFNVLVGGFFSAKRCEAAIPLNAWVE
CCEEEEEEEECCCCCCEEEECCEEEEECCCCCCCCEEEEEECCCCHHHCCEECCCCCCCC
PNQVIALCRAILEVYRDYGLRENRQKSRLMWLIDEWGLTKFRSQVEKQIGLTLETEALKD
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCEEEHHHHHH
EILWEKRDHIGIYKQKQPGLNYIGLNIPVGRLYAVDMFDLARLAEVYGNGEIRLTVEQNV
HHHHHCCCCCCEEECCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCCEEEEEEECCE
IIPNVPDSRLETFRSEPLLKKFSFEPQPLTRAVVSCTGSQFCNFALIETKNRALAIAKEL
EECCCCHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHCCCHHCCEEEEECCCCHHHHHHHH
ETELSIPKPVRIHWTGCPNSCGQPQVADIGLMGTKVRKNGKMVDGVNLYMGGKVGKDAQL
HHHCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEECCCCCEECCEEEEECCCCCCCCHH
GSCVQKSIPCEDLKPILRSLLIKNFAAQPK
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766873 [H]