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The map label for this gene is mnhD [H]

Identifier: 110835517

GI number: 110835517

Start: 3000146

End: 3001627

Strand: Reverse

Name: mnhD [H]

Synonym: ABO_2656

Alternate gene names: 110835517

Gene position: 3001627-3000146 (Counterclockwise)

Preceding gene: 110835518

Following gene: 110835516

Centisome position: 96.2

GC content: 58.64

Gene sequence:

>1482_bases
ATGAGCCACTGGATTATTGCCCCGATACTGATACCGGCGCTGGCGGGTATGTTGCTGTTGCTGGAGGTGCGCGAGCGCCA
CCGGTTGCGGCGGATCACCGGCCTGCTGTCCTGCTCGGCGCTGCTGGCGGCGGCTATTTTTCTGGGCAATCAGGCCTGGG
GCGGGGACATCACCGTTTACGCGCTGGGTAACTGGCAGGCGCCGTTCGGTATTGTGCTGGTGCTGGATAAGCTCAGTGCC
TTGATGGTACTGCTGACGGCAGTATTGGCGTTCCCTGCGCTGCTGTACGCTAGCTGCGGTGATGACCGCAAAGGCGCGAA
TTTCCATGCCTTGTTTCAATTTCAGCTGATGGGTATCAACGGCGCCTTCCTCACCGGAGACTTGTTCAATCTGTTCGTAT
TTTTCGAAGTGCTGCTGATTGCCTCTTATGGCTTGGCTCTGCACGGCCGAGGCCCGGAGCGAGTAAAGGCAGGCTTGCAT
TATGTGGTGCTTAATTTGGTGGGCTCGGCGCTGTTCTTAATCGCGTTGGGGTTGATTTACGGAACCACCGGCACGCTTAA
CATGGCGGACTTTGCGGTGAAGGCCCAGGCGCTTACCGGCACCCATGCCGTCTTGTTAAAGGTAGCGGTGTTGATGCTGT
GGGTGGTGTTTGGGCTCAAGGCGGCATTGTTTCCATTGTATTTCTGGCTGCCAGCGGCCTATGCCGGTGCCACCGCCGGG
GTGGCCGCATTGTTTGCAATTATGACCAAGGTGGGCTTTTACGCCATGCTGCGGGTTTATGCGCTGGCGTTTGCCGATGG
CCCCTTTGCTGGACTGGGGCAGGCGTCACTTTGGTGGTTGTCGTTGGTGACATTGGCATTGGCTTGCATTGGCGTGCTGG
GCGCTAAACGGCTGGGCACACTGGTGTCGTACCTGGTAGTGATGTCTGTGGGTACATTAGTGGCGGCCTTGGCCTTCGGT
GAGCTACAGGCGGTGGGCCCGGCGCTGTACTACCTGCTGCATTCCACCCTGATTTGCGGTGGCTTGTTTCTGCTTACGGA
CTTGATTGCCCGTGGTCGAGGCGACGTGGCGGATTACCTGCAGATTGGCCCGGCGATTCTTAATGGCGGTCGGCTGTCAC
TGTTGTTTTTTGTGGCGGCGATTGCGGTGGTGGGTTTGCCGCCTCTCTCTGGTTTTGTCGGTAAAGTGGCGTTACTGCGC
AGCGTACCGGATCCGACTTATTGGGTGTTGCTGCTGCTGGCGGGGCTGTGTGGTTTGATTGCCCTTAGCCGGGCAGGTTC
TACCTTGTTCTGGCGCACCACCGGGGAGGGCCGCGGCCTACGCCTGGGCCACCGGCGTTTGTTTCCCGTAGTGGCGTTGC
TGATGGCGACCCCGCTGATGGCCGTGTATGCGGAACTTTTGTTGCAACTAACCGCTGCGGTTGCGGAGCAGCTGCGTAAT
CCGGACTTATATTTGTCAGCAGTATTGGGAGGTGGGGCATGA

Upstream 100 bases:

>100_bases
GCTTCGCTATGACGGCGTTTTTGGTAGTGCTGGCCTTGCGTGGCCAGGGAGAGCTGCACAGTGATCATGTGGATGGCAAG
CCGGATGAAGGGGGCCGTTC

Downstream 100 bases:

>100_bases
AGTTACTGTATCGCGTGCTGCCTTACCCCAGCCTGAGCCTGATTCTGTGGTTCGCCTGGATGCTGCTTAGCGGTTTCACT
GCCGGGCATGCGGTGCTGGG

Product: multisubunit Na+/H+ antiporter, MnhD subunit

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSHWIIAPILIPALAGMLLLLEVRERHRLRRITGLLSCSALLAAAIFLGNQAWGGDITVYALGNWQAPFGIVLVLDKLSA
LMVLLTAVLAFPALLYASCGDDRKGANFHALFQFQLMGINGAFLTGDLFNLFVFFEVLLIASYGLALHGRGPERVKAGLH
YVVLNLVGSALFLIALGLIYGTTGTLNMADFAVKAQALTGTHAVLLKVAVLMLWVVFGLKAALFPLYFWLPAAYAGATAG
VAALFAIMTKVGFYAMLRVYALAFADGPFAGLGQASLWWLSLVTLALACIGVLGAKRLGTLVSYLVVMSVGTLVAALAFG
ELQAVGPALYYLLHSTLICGGLFLLTDLIARGRGDVADYLQIGPAILNGGRLSLLFFVAAIAVVGLPPLSGFVGKVALLR
SVPDPTYWVLLLLAGLCGLIALSRAGSTLFWRTTGEGRGLRLGHRRLFPVVALLMATPLMAVYAELLLQLTAAVAEQLRN
PDLYLSAVLGGGA

Sequences:

>Translated_493_residues
MSHWIIAPILIPALAGMLLLLEVRERHRLRRITGLLSCSALLAAAIFLGNQAWGGDITVYALGNWQAPFGIVLVLDKLSA
LMVLLTAVLAFPALLYASCGDDRKGANFHALFQFQLMGINGAFLTGDLFNLFVFFEVLLIASYGLALHGRGPERVKAGLH
YVVLNLVGSALFLIALGLIYGTTGTLNMADFAVKAQALTGTHAVLLKVAVLMLWVVFGLKAALFPLYFWLPAAYAGATAG
VAALFAIMTKVGFYAMLRVYALAFADGPFAGLGQASLWWLSLVTLALACIGVLGAKRLGTLVSYLVVMSVGTLVAALAFG
ELQAVGPALYYLLHSTLICGGLFLLTDLIARGRGDVADYLQIGPAILNGGRLSLLFFVAAIAVVGLPPLSGFVGKVALLR
SVPDPTYWVLLLLAGLCGLIALSRAGSTLFWRTTGEGRGLRLGHRRLFPVVALLMATPLMAVYAELLLQLTAAVAEQLRN
PDLYLSAVLGGGA
>Mature_492_residues
SHWIIAPILIPALAGMLLLLEVRERHRLRRITGLLSCSALLAAAIFLGNQAWGGDITVYALGNWQAPFGIVLVLDKLSAL
MVLLTAVLAFPALLYASCGDDRKGANFHALFQFQLMGINGAFLTGDLFNLFVFFEVLLIASYGLALHGRGPERVKAGLHY
VVLNLVGSALFLIALGLIYGTTGTLNMADFAVKAQALTGTHAVLLKVAVLMLWVVFGLKAALFPLYFWLPAAYAGATAGV
AALFAIMTKVGFYAMLRVYALAFADGPFAGLGQASLWWLSLVTLALACIGVLGAKRLGTLVSYLVVMSVGTLVAALAFGE
LQAVGPALYYLLHSTLICGGLFLLTDLIARGRGDVADYLQIGPAILNGGRLSLLFFVAAIAVVGLPPLSGFVGKVALLRS
VPDPTYWVLLLLAGLCGLIALSRAGSTLFWRTTGEGRGLRLGHRRLFPVVALLMATPLMAVYAELLLQLTAAVAEQLRNP
DLYLSAVLGGGA

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=161, Percent_Identity=34.1614906832298, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788613, Length=416, Percent_Identity=27.8846153846154, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788827, Length=353, Percent_Identity=26.9121813031161, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 52214; Mature: 52083

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHWIIAPILIPALAGMLLLLEVRERHRLRRITGLLSCSALLAAAIFLGNQAWGGDITVY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
ALGNWQAPFGIVLVLDKLSALMVLLTAVLAFPALLYASCGDDRKGANFHALFQFQLMGIN
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCC
GAFLTGDLFNLFVFFEVLLIASYGLALHGRGPERVKAGLHYVVLNLVGSALFLIALGLIY
CCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTGTLNMADFAVKAQALTGTHAVLLKVAVLMLWVVFGLKAALFPLYFWLPAAYAGATAG
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VAALFAIMTKVGFYAMLRVYALAFADGPFAGLGQASLWWLSLVTLALACIGVLGAKRLGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVSYLVVMSVGTLVAALAFGELQAVGPALYYLLHSTLICGGLFLLTDLIARGRGDVADYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
QIGPAILNGGRLSLLFFVAAIAVVGLPPLSGFVGKVALLRSVPDPTYWVLLLLAGLCGLI
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALSRAGSTLFWRTTGEGRGLRLGHRRLFPVVALLMATPLMAVYAELLLQLTAAVAEQLRN
HHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDLYLSAVLGGGA
CCEEEEEECCCCC
>Mature Secondary Structure 
SHWIIAPILIPALAGMLLLLEVRERHRLRRITGLLSCSALLAAAIFLGNQAWGGDITVY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE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CCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]