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The map label for this gene is fadI [H]

Identifier: 110835313

GI number: 110835313

Start: 2775330

End: 2776631

Strand: Direct

Name: fadI [H]

Synonym: ABO_2452

Alternate gene names: 110835313

Gene position: 2775330-2776631 (Clockwise)

Preceding gene: 110835311

Following gene: 110835314

Centisome position: 88.95

GC content: 60.52

Gene sequence:

>1302_bases
ATGCCTGTTCGCAAAATGAAGCTGGCTGGCCGTGGCGGCCGCGTCGCCATCGTAGATGGTCTGCGCACCCCGTTTGCTCG
CATCGCCACCCATTACCGAGATTTGAATGCCATCGATCTAGGCGCAATGGCCGTCAAGGAGCTGATGGCCCGCAATAACC
TGAGCCAAAATGAAATTGAGCAGCTGGTGTTCGGGATGACGGTGATGATTCCCGAAGCCCCCTTTATTGCCCGCGAGATC
GCTCTGGCGCTGGGCATGGACACGGTGGATGCCTACTCCATTACCCGTGCCTGTGCCACCAGTTTCCAGACCACCGCCAG
CGTGGCGGACAACATCGTTGCCGGTTACACCGACATGGCCATTGCTGGCGGGACTGACTCCACCAGCTGCGCTCGAATTC
CCTTGTCTGCCAAGCTAAGCGCGGTACTGCGCGATGTGAGCTTCGCCAAAACCCTAAAAGATCGCATCAAGCTGCTTAGC
CAGCTCAAAGCCCGCGACCTGCTTCCCCAAGCCCCGTCTATCACCGAATACTCAGTGGGGGAAACCATGGGCGAGAGCAC
CGAGAAAATGGTGAAAAAATGGGGGGTTAGCCGTCAAGAACAAGACGATCTAGCCCATGCTTCCCACAGCAATGCAGCCC
GCGCCTGGGCCGAAGGCCGTCTGGATCGCCAGGTGATGACTGCCCATTTACCCGATGGCAGCGCCTTGAAAGATGACAAC
CTGGTGCGCAAACAATCCGAACGTGAGAGCTACAGCAAACTCAATCCGGTGTTTGACCGGGAGACCGGTTCCGTTACCGC
CGGCAATAGCAGCCCCCTCACCGATGGTGCCGGCGCCCTGTTGATGATGAGCGAGAAAAAGGCCGCCGCACTGGGTTACA
CCCCGCTAGGTTATATTCGCTCCTATGCCTTTGCCGCCCGCACGCCCCAGGACGACCTATTAATGGGCCCGGTACTGGCC
GCCCCCGCCGCCATGACCCGCGCCGGGCTCACCTTGAGCGACCTGACCCTGGTAGACATGCACGAAGCGTTTGCCGGCCA
AGTAGCCTGCAACCTGCGTGGCTTGGCCGATGACGACTATGTACGCAGCCAGACTGGGCTCGCGGCTCTCGGCGAAGTAG
ATCGCAGCAAGCTCAACGTCAACGGCGGCTCCATTGCCTACGGCCACCCCTTTGGGGCCACCGGTGCCCGAGTCATCAGC
CAGACCTTACACGAACTGCAACACCGCGGCGGCGGGCTGGCGTTAACCACTGCCTGTGCCGCCGGCGGTATCGGCGCCGC
CATGGTGCTGGAAACCGAATAA

Upstream 100 bases:

>100_bases
CAGTGCAGCCTTCTAGGCTCCACCCACGTTATCACCAATGAATGTCACATTAGGTGTAAAACAGATTACACCGTGACCGG
CACCCTGCAAGGAGTCTGCA

Downstream 100 bases:

>100_bases
CCCGCGTTAGCCACATTACTTAGCGCACCACACAACCACCCACAGGAACAGACCCATGTTTAACCTACTGATGAATTGGA
TGCGGGATAACAAGATCCTG

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MPVRKMKLAGRGGRVAIVDGLRTPFARIATHYRDLNAIDLGAMAVKELMARNNLSQNEIEQLVFGMTVMIPEAPFIAREI
ALALGMDTVDAYSITRACATSFQTTASVADNIVAGYTDMAIAGGTDSTSCARIPLSAKLSAVLRDVSFAKTLKDRIKLLS
QLKARDLLPQAPSITEYSVGETMGESTEKMVKKWGVSRQEQDDLAHASHSNAARAWAEGRLDRQVMTAHLPDGSALKDDN
LVRKQSERESYSKLNPVFDRETGSVTAGNSSPLTDGAGALLMMSEKKAAALGYTPLGYIRSYAFAARTPQDDLLMGPVLA
APAAMTRAGLTLSDLTLVDMHEAFAGQVACNLRGLADDDYVRSQTGLAALGEVDRSKLNVNGGSIAYGHPFGATGARVIS
QTLHELQHRGGGLALTTACAAGGIGAAMVLETE

Sequences:

>Translated_433_residues
MPVRKMKLAGRGGRVAIVDGLRTPFARIATHYRDLNAIDLGAMAVKELMARNNLSQNEIEQLVFGMTVMIPEAPFIAREI
ALALGMDTVDAYSITRACATSFQTTASVADNIVAGYTDMAIAGGTDSTSCARIPLSAKLSAVLRDVSFAKTLKDRIKLLS
QLKARDLLPQAPSITEYSVGETMGESTEKMVKKWGVSRQEQDDLAHASHSNAARAWAEGRLDRQVMTAHLPDGSALKDDN
LVRKQSERESYSKLNPVFDRETGSVTAGNSSPLTDGAGALLMMSEKKAAALGYTPLGYIRSYAFAARTPQDDLLMGPVLA
APAAMTRAGLTLSDLTLVDMHEAFAGQVACNLRGLADDDYVRSQTGLAALGEVDRSKLNVNGGSIAYGHPFGATGARVIS
QTLHELQHRGGGLALTTACAAGGIGAAMVLETE
>Mature_432_residues
PVRKMKLAGRGGRVAIVDGLRTPFARIATHYRDLNAIDLGAMAVKELMARNNLSQNEIEQLVFGMTVMIPEAPFIAREIA
LALGMDTVDAYSITRACATSFQTTASVADNIVAGYTDMAIAGGTDSTSCARIPLSAKLSAVLRDVSFAKTLKDRIKLLSQ
LKARDLLPQAPSITEYSVGETMGESTEKMVKKWGVSRQEQDDLAHASHSNAARAWAEGRLDRQVMTAHLPDGSALKDDNL
VRKQSERESYSKLNPVFDRETGSVTAGNSSPLTDGAGALLMMSEKKAAALGYTPLGYIRSYAFAARTPQDDLLMGPVLAA
PAAMTRAGLTLSDLTLVDMHEAFAGQVACNLRGLADDDYVRSQTGLAALGEVDRSKLNVNGGSIAYGHPFGATGARVISQ
TLHELQHRGGGLALTTACAAGGIGAAMVLETE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=419, Percent_Identity=37.708830548926, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI167614485, Length=423, Percent_Identity=30.9692671394799, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=31.8604651162791, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI4557237, Length=424, Percent_Identity=27.8301886792453, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI148539872, Length=424, Percent_Identity=27.122641509434, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=55.868544600939, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1788554, Length=419, Percent_Identity=32.9355608591885, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87082165, Length=425, Percent_Identity=31.5294117647059, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787663, Length=425, Percent_Identity=32.4705882352941, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI48994986, Length=429, Percent_Identity=31.9347319347319, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=36.9668246445498, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI133906874, Length=424, Percent_Identity=28.5377358490566, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17535921, Length=424, Percent_Identity=27.8301886792453, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=26.1904761904762, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17535917, Length=423, Percent_Identity=22.6950354609929, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6325229, Length=431, Percent_Identity=29.4663573085847, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=421, Percent_Identity=26.3657957244656, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17137578, Length=419, Percent_Identity=37.9474940334129, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24655093, Length=421, Percent_Identity=28.5035629453682, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=27.6995305164319, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17648125, Length=423, Percent_Identity=27.6595744680851, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 45947; Mature: 45816

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVRKMKLAGRGGRVAIVDGLRTPFARIATHYRDLNAIDLGAMAVKELMARNNLSQNEIE
CCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGMTVMIPEAPFIAREIALALGMDTVDAYSITRACATSFQTTASVADNIVAGYTDMA
HHHHHHHEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IAGGTDSTSCARIPLSAKLSAVLRDVSFAKTLKDRIKLLSQLKARDLLPQAPSITEYSVG
EECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ETMGESTEKMVKKWGVSRQEQDDLAHASHSNAARAWAEGRLDRQVMTAHLPDGSALKDDN
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHEEECCCCCCCCCCH
LVRKQSERESYSKLNPVFDRETGSVTAGNSSPLTDGAGALLMMSEKKAAALGYTPLGYIR
HHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCHHHCCCCHHHHHH
SYAFAARTPQDDLLMGPVLAAPAAMTRAGLTLSDLTLVDMHEAFAGQVACNLRGLADDDY
HHHHHCCCCCCCHHHCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VRSQTGLAALGEVDRSKLNVNGGSIAYGHPFGATGARVISQTLHELQHRGGGLALTTACA
HHHHCCHHHHHCCCHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
AGGIGAAMVLETE
CCCCCEEEEEECC
>Mature Secondary Structure 
PVRKMKLAGRGGRVAIVDGLRTPFARIATHYRDLNAIDLGAMAVKELMARNNLSQNEIE
CCCCEEECCCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
QLVFGMTVMIPEAPFIAREIALALGMDTVDAYSITRACATSFQTTASVADNIVAGYTDMA
HHHHHHHEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IAGGTDSTSCARIPLSAKLSAVLRDVSFAKTLKDRIKLLSQLKARDLLPQAPSITEYSVG
EECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ETMGESTEKMVKKWGVSRQEQDDLAHASHSNAARAWAEGRLDRQVMTAHLPDGSALKDDN
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHEEECCCCCCCCCCH
LVRKQSERESYSKLNPVFDRETGSVTAGNSSPLTDGAGALLMMSEKKAAALGYTPLGYIR
HHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECCCHHHCCCCHHHHHH
SYAFAARTPQDDLLMGPVLAAPAAMTRAGLTLSDLTLVDMHEAFAGQVACNLRGLADDDY
HHHHHCCCCCCCHHHCHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VRSQTGLAALGEVDRSKLNVNGGSIAYGHPFGATGARVISQTLHELQHRGGGLALTTACA
HHHHCCHHHHHCCCHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHH
AGGIGAAMVLETE
CCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA