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The map label for this gene is epsE [H]

Identifier: 110835164

GI number: 110835164

Start: 2627311

End: 2629122

Strand: Reverse

Name: epsE [H]

Synonym: ABO_2303

Alternate gene names: 110835164

Gene position: 2629122-2627311 (Counterclockwise)

Preceding gene: 110835168

Following gene: 110835163

Centisome position: 84.26

GC content: 56.29

Gene sequence:

>1812_bases
GTGAAGAAAGTGACGGTTAGCGCTCCTGGGGATGCCCCCAAGCTGGACGGCCTTCTGGTTGATGTGCGCTGGTTGATGCG
TGAATTGCTGGATGAAGGGCGCGTCAGTCAGGAGGACTTTAACGTGATCTCCACGACGCCGCGGGAAAAGAAAGAATTGA
ATTGGCACCCTATTCAGGTGCTGGCTAAGTACCGCCTTACCGATCGCACCAATACCCACCAGCTTTTGGATGTGGACTTT
CTGACTCATTGGCTGTCAGAAAAAAGCGGCTTGCCGGTGTACAGCATTGATCCGCTTAAAGTGCATGTGGATCAGGTGAC
CAATGTAATGAGTTACGCCTTCGCTGAACGCCACGGTATTGTGGCGGTGGAGGTGCATCGCGATAAGGTGGTGGTGGCCT
GCGACCAGCCGTTCGTGCGCGGCTGGCAGCCGCATTTGCAACAGGTGCTGCGCGATCGTGAGCTGGAAGTGGTGTTGGTT
AATCCGGAGCAAATGCGCCGCTACCGGATTGAGTTCTATAACCTGAGCCGCTCCATTGCGGGGGCGACCGGCAACACCGG
GCAGGAAATGTCGGGCATTACCAACTTCGAGCAGCTACTCGAAGTGGGCAAAGGGCACCACGATGCGGACGACCAGCATA
TCGTTAACATTGTGGACTGGATTCTGCAGTATGCGTTTTCCCAGCGCGCCAGCGATATCCACCTGGAGCCACGCCGGGAA
ACCGGCAAGATGCGCTTTCGCATTGATGGTGTGCTGCATGATGTGTATGAATTGCCGGCGGCCATTATGGCGGCGGTGAC
TAGCCGGCTAAAGATTCTTAGCCGTTTAAATGTGGCGGAAAAGCGTAAACCCCAGGATGGTCGCTTAAAGACCAAATCGC
CGGAAGGGGATGAGATTGAGCTGCGGATTTCTACTCTGCCCACCGCGTTCGGTGAAAAGATGGTGATGCGGGTATTCGAC
CCGGATGTGTTGGTGCGTAGCTTCGAGCAATTGGGTTTTAGCAAGGAGGATCACGATACCTGGCACCAGATGACCAGCCA
CCCCCATGGCATTATTTTTGTCACCGGCCCCACCGGGTCGGGGAAAACCACCACGCTGTATTCTACGCTCAAGCAGTTGG
CCACCAGCGAAGTGAATGTTTGCACCATCGAAGACCCCATCGAAATGGTGGAGTCCAGTTTTAACCAGATGCAAATTCAG
GCCAATATTGATGTGGACTTTGCCCAAGGCGTGAAGGCCATGCTGCGCCAAGACCCGGATATCATCATGATTGGTGAGAT
TCGCGACTTGCAGACTGCGGACATGGCCATTCAGGCTGCCCTCACCGGCCACCTGGTGCTGTCGACCCTGCATACCAATG
ACGCGCCATCGTCCGTCACCCGGTTGATCGACCTAGGCGTGGCGCCTTATATGATTAGCGCCACGGTGCTGGGTGTGATG
GCGCAACGGCTGGTGCGAACGCTGTGCCCGAACTGCAAGACGGCCACCGAAACCGACCCGCAAGCCTGGGAGGAGCTGGT
TCGTCCTTTCAAGATGAAGATGCCGGCAACGCTGTATCAGCCGGTGGGTTGCTTGGAATGTCGAGGGACTGGGTATCGAG
GCCGGATGGGGGTGTATGAAACCATGCCCCTGCGCACTACCCTGAAAAACCACATCACCCGTGGCGCCGACCTGGAAACC
CTGACCCGGGCCTCACTCAAAGAAGGCATGAAACCCCTGCGAATCAGTGGCGCCATAAAAGTGGCCAAGGGGCTGACGAC
CATTGAAGAGGTTATGCGGGTGACGCCCTCCCACGATATTTTGATGGGGTAG

Upstream 100 bases:

>100_bases
CCTACGCCAGAATAATGAGATGGCTCGGCTTGCCACAGATGGTGTTTTGCCGTGTGGCGCAGGCCAATAGAGCCCCGGGT
AGAGTTGGAGAGTATAGAAA

Downstream 100 bases:

>100_bases
CGCTGCATCAATCGCGGCAGCAATAAGGAGAGCGCGCGGGCGAGAATGTTCTGGGTTTGCCCTGAGGGGCCCAAGTTCCA
GCGCTGGGCTCCGTTGGTTG

Product: type II secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MKKVTVSAPGDAPKLDGLLVDVRWLMRELLDEGRVSQEDFNVISTTPREKKELNWHPIQVLAKYRLTDRTNTHQLLDVDF
LTHWLSEKSGLPVYSIDPLKVHVDQVTNVMSYAFAERHGIVAVEVHRDKVVVACDQPFVRGWQPHLQQVLRDRELEVVLV
NPEQMRRYRIEFYNLSRSIAGATGNTGQEMSGITNFEQLLEVGKGHHDADDQHIVNIVDWILQYAFSQRASDIHLEPRRE
TGKMRFRIDGVLHDVYELPAAIMAAVTSRLKILSRLNVAEKRKPQDGRLKTKSPEGDEIELRISTLPTAFGEKMVMRVFD
PDVLVRSFEQLGFSKEDHDTWHQMTSHPHGIIFVTGPTGSGKTTTLYSTLKQLATSEVNVCTIEDPIEMVESSFNQMQIQ
ANIDVDFAQGVKAMLRQDPDIIMIGEIRDLQTADMAIQAALTGHLVLSTLHTNDAPSSVTRLIDLGVAPYMISATVLGVM
AQRLVRTLCPNCKTATETDPQAWEELVRPFKMKMPATLYQPVGCLECRGTGYRGRMGVYETMPLRTTLKNHITRGADLET
LTRASLKEGMKPLRISGAIKVAKGLTTIEEVMRVTPSHDILMG

Sequences:

>Translated_603_residues
MKKVTVSAPGDAPKLDGLLVDVRWLMRELLDEGRVSQEDFNVISTTPREKKELNWHPIQVLAKYRLTDRTNTHQLLDVDF
LTHWLSEKSGLPVYSIDPLKVHVDQVTNVMSYAFAERHGIVAVEVHRDKVVVACDQPFVRGWQPHLQQVLRDRELEVVLV
NPEQMRRYRIEFYNLSRSIAGATGNTGQEMSGITNFEQLLEVGKGHHDADDQHIVNIVDWILQYAFSQRASDIHLEPRRE
TGKMRFRIDGVLHDVYELPAAIMAAVTSRLKILSRLNVAEKRKPQDGRLKTKSPEGDEIELRISTLPTAFGEKMVMRVFD
PDVLVRSFEQLGFSKEDHDTWHQMTSHPHGIIFVTGPTGSGKTTTLYSTLKQLATSEVNVCTIEDPIEMVESSFNQMQIQ
ANIDVDFAQGVKAMLRQDPDIIMIGEIRDLQTADMAIQAALTGHLVLSTLHTNDAPSSVTRLIDLGVAPYMISATVLGVM
AQRLVRTLCPNCKTATETDPQAWEELVRPFKMKMPATLYQPVGCLECRGTGYRGRMGVYETMPLRTTLKNHITRGADLET
LTRASLKEGMKPLRISGAIKVAKGLTTIEEVMRVTPSHDILMG
>Mature_603_residues
MKKVTVSAPGDAPKLDGLLVDVRWLMRELLDEGRVSQEDFNVISTTPREKKELNWHPIQVLAKYRLTDRTNTHQLLDVDF
LTHWLSEKSGLPVYSIDPLKVHVDQVTNVMSYAFAERHGIVAVEVHRDKVVVACDQPFVRGWQPHLQQVLRDRELEVVLV
NPEQMRRYRIEFYNLSRSIAGATGNTGQEMSGITNFEQLLEVGKGHHDADDQHIVNIVDWILQYAFSQRASDIHLEPRRE
TGKMRFRIDGVLHDVYELPAAIMAAVTSRLKILSRLNVAEKRKPQDGRLKTKSPEGDEIELRISTLPTAFGEKMVMRVFD
PDVLVRSFEQLGFSKEDHDTWHQMTSHPHGIIFVTGPTGSGKTTTLYSTLKQLATSEVNVCTIEDPIEMVESSFNQMQIQ
ANIDVDFAQGVKAMLRQDPDIIMIGEIRDLQTADMAIQAALTGHLVLSTLHTNDAPSSVTRLIDLGVAPYMISATVLGVM
AQRLVRTLCPNCKTATETDPQAWEELVRPFKMKMPATLYQPVGCLECRGTGYRGRMGVYETMPLRTTLKNHITRGADLET
LTRASLKEGMKPLRISGAIKVAKGLTTIEEVMRVTPSHDILMG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=386, Percent_Identity=43.7823834196891, Blast_Score=332, Evalue=5e-92,
Organism=Escherichia coli, GI1786296, Length=373, Percent_Identity=42.627345844504, Blast_Score=299, Evalue=4e-82,
Organism=Escherichia coli, GI87082188, Length=184, Percent_Identity=35.8695652173913, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 67875; Mature: 67875

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVTVSAPGDAPKLDGLLVDVRWLMRELLDEGRVSQEDFNVISTTPREKKELNWHPIQV
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCEECCCCCHHHCCCCCHHHH
LAKYRLTDRTNTHQLLDVDFLTHWLSEKSGLPVYSIDPLKVHVDQVTNVMSYAFAERHGI
HHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCEEECCHHEEEHHHHHHHHHHHHHHHCCE
VAVEVHRDKVVVACDQPFVRGWQPHLQQVLRDRELEVVLVNPEQMRRYRIEFYNLSRSIA
EEEEEECCEEEEEECCHHHCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHC
GATGNTGQEMSGITNFEQLLEVGKGHHDADDQHIVNIVDWILQYAFSQRASDIHLEPRRE
CCCCCCCHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TGKMRFRIDGVLHDVYELPAAIMAAVTSRLKILSRLNVAEKRKPQDGRLKTKSPEGDEIE
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCEEE
LRISTLPTAFGEKMVMRVFDPDVLVRSFEQLGFSKEDHDTWHQMTSHPHGIIFVTGPTGS
EEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCC
GKTTTLYSTLKQLATSEVNVCTIEDPIEMVESSFNQMQIQANIDVDFAQGVKAMLRQDPD
CCCHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCC
IIMIGEIRDLQTADMAIQAALTGHLVLSTLHTNDAPSSVTRLIDLGVAPYMISATVLGVM
EEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
AQRLVRTLCPNCKTATETDPQAWEELVRPFKMKMPATLYQPVGCLECRGTGYRGRMGVYE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCCCCCCCCCCC
TMPLRTTLKNHITRGADLETLTRASLKEGMKPLRISGAIKVAKGLTTIEEVMRVTPSHDI
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCC
LMG
CCC
>Mature Secondary Structure
MKKVTVSAPGDAPKLDGLLVDVRWLMRELLDEGRVSQEDFNVISTTPREKKELNWHPIQV
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCEECCCCCHHHCCCCCHHHH
LAKYRLTDRTNTHQLLDVDFLTHWLSEKSGLPVYSIDPLKVHVDQVTNVMSYAFAERHGI
HHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCEEECCHHEEEHHHHHHHHHHHHHHHCCE
VAVEVHRDKVVVACDQPFVRGWQPHLQQVLRDRELEVVLVNPEQMRRYRIEFYNLSRSIA
EEEEEECCEEEEEECCHHHCCCHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHC
GATGNTGQEMSGITNFEQLLEVGKGHHDADDQHIVNIVDWILQYAFSQRASDIHLEPRRE
CCCCCCCHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TGKMRFRIDGVLHDVYELPAAIMAAVTSRLKILSRLNVAEKRKPQDGRLKTKSPEGDEIE
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCCEEE
LRISTLPTAFGEKMVMRVFDPDVLVRSFEQLGFSKEDHDTWHQMTSHPHGIIFVTGPTGS
EEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCC
GKTTTLYSTLKQLATSEVNVCTIEDPIEMVESSFNQMQIQANIDVDFAQGVKAMLRQDPD
CCCHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHCCCC
IIMIGEIRDLQTADMAIQAALTGHLVLSTLHTNDAPSSVTRLIDLGVAPYMISATVLGVM
EEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
AQRLVRTLCPNCKTATETDPQAWEELVRPFKMKMPATLYQPVGCLECRGTGYRGRMGVYE
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCCCCCCCCCCC
TMPLRTTLKNHITRGADLETLTRASLKEGMKPLRISGAIKVAKGLTTIEEVMRVTPSHDI
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCC
LMG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]