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The map label for this gene is dnaB [H]

Identifier: 110835048

GI number: 110835048

Start: 2497161

End: 2498555

Strand: Reverse

Name: dnaB [H]

Synonym: ABO_2187

Alternate gene names: 110835048

Gene position: 2498555-2497161 (Counterclockwise)

Preceding gene: 110835049

Following gene: 110835047

Centisome position: 80.08

GC content: 55.13

Gene sequence:

>1395_bases
ATGAATGAACCCATGTCGCTGGACAAACATCTGCCTGAAAACCTGCGTATTCCCCCCAACTCTGTGGAGGCGGAGCAGGC
GGTGCTGGGTGGTTTGCTGCTCAATAACAGCGCCTGGGATGATGTGGCCGAGCGCGTTGGCGCGAGGGACTTTTACCGTA
AGGAGCATCGCCAGATTTTCGAGGTGATTGCCCAGCTGGTGGAAGAAGAAAACCCCAGTGACCTGGTTACCGTCTCCCAG
GCGCTGAATAACCTGGGCCAGTTGGAATCCATCGGCGGCATGACCTACCTGTCTGAGTTGGCCCGCAACACTCCCAGTGC
GGCCAACATTACTGCCTATTCGGATATTGTCCGTGAGCGCAGCATCCTGCGGCAGTTGATCAATGTGTCCCACAAGGTGG
CAGACAGCGCGTTTAATACAGAGGGCCGCAAGTCATTGGAAATTCTTGATGAAGCGGAATCCGCCATCTTCAAGATTGCT
GAGCAGCAGAAAAAAGGCGATGGCCCGCAGAACATCAAATCAGTGCTTAAAAAAGCAGTGGACCGGATTGATGAGCTGTA
CAAAACGAAGAGTTCACTTACCGGCCTGACGTCTGGTTTTGAAGAGTTGGATAAAATGACCGGGGGCCTGCAGCCGGCGG
ACATGATCGTGGTTGCGGGCCGGCCTTCCATGGGTAAGACCACCTTTGCCATGAACCTGTGTGAAAACGTGGCGATCAAG
AGTAACAAGCCCGTGCTGGTGTTCTCCATGGAAATGCCGGCGGAGTCCATCGTAATGCGGATGTTGTCCTCGCTGGGCCG
CATCAATCAGGGGGCAATTCGTTCCGGTAATCTGGAAAAGGATGATTGGCCGCGCATTACTTCTGCCATTCACATGCTCA
GTGAGCAACGCTTTTTTATTGATGATACCCCGGCACTGAGCCCGCTGGAGATGCGTGCCCGTGCTCGTCGGGTAGCGCGC
GAATGTGGCGGTGAGCTGGGCCTGATCATGGTCGATTACCTGCAGCTGATGCAGGTGCCTGGGGTGGAAAACCGGGTGAA
CGAAATCTCGGAAATTTCCCGCTCCCTAAAAGGGATCGCTAAGGAGCTGAATTGTCCGGTGCTGGCGCTCTCCCAGTTGA
ACCGCTCGCTGGAACAACGCCCCAACAAACGCCCTGTGATGTCGGATCTACGTGAATCCGGGGCGATTGAGCAGGATGCG
GATTTGATCGTCTTCCTGTACCGGGACGAGGTGTATAACAAGGACAGCAATGAGCGCGGTGTCGCCGAGATTATTATCGG
CAAACAGCGTAATGGTCCTATCGGTACCGTACGCCTAGCCTTCCGCGGTGAGTTCACCCGCTTCGATGATCTGGCCCCGG
AATATTATGCGCAGTTGGCCGCAGGGGATGAATAA

Upstream 100 bases:

>100_bases
GCCTAGCGTTTTGGCATGGATAAGGCCTTTCAAATGATTATGCCTTCTTTCTTGTTATGGTTTGTAGCTTGTAGCTCAAA
ACTTGGCTGTAGTTATTGCT

Downstream 100 bases:

>100_bases
AACGCAGAATGAAGGCGGTTGGATGCTGAATGCGCAGCCCTCTTTTTTAAAGGTTAACCGTCAGCCACTAGGCGCTGGAC
TGCAATTTTTATGAGAGGCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNEPMSLDKHLPENLRIPPNSVEAEQAVLGGLLLNNSAWDDVAERVGARDFYRKEHRQIFEVIAQLVEEENPSDLVTVSQ
ALNNLGQLESIGGMTYLSELARNTPSAANITAYSDIVRERSILRQLINVSHKVADSAFNTEGRKSLEILDEAESAIFKIA
EQQKKGDGPQNIKSVLKKAVDRIDELYKTKSSLTGLTSGFEELDKMTGGLQPADMIVVAGRPSMGKTTFAMNLCENVAIK
SNKPVLVFSMEMPAESIVMRMLSSLGRINQGAIRSGNLEKDDWPRITSAIHMLSEQRFFIDDTPALSPLEMRARARRVAR
ECGGELGLIMVDYLQLMQVPGVENRVNEISEISRSLKGIAKELNCPVLALSQLNRSLEQRPNKRPVMSDLRESGAIEQDA
DLIVFLYRDEVYNKDSNERGVAEIIIGKQRNGPIGTVRLAFRGEFTRFDDLAPEYYAQLAAGDE

Sequences:

>Translated_464_residues
MNEPMSLDKHLPENLRIPPNSVEAEQAVLGGLLLNNSAWDDVAERVGARDFYRKEHRQIFEVIAQLVEEENPSDLVTVSQ
ALNNLGQLESIGGMTYLSELARNTPSAANITAYSDIVRERSILRQLINVSHKVADSAFNTEGRKSLEILDEAESAIFKIA
EQQKKGDGPQNIKSVLKKAVDRIDELYKTKSSLTGLTSGFEELDKMTGGLQPADMIVVAGRPSMGKTTFAMNLCENVAIK
SNKPVLVFSMEMPAESIVMRMLSSLGRINQGAIRSGNLEKDDWPRITSAIHMLSEQRFFIDDTPALSPLEMRARARRVAR
ECGGELGLIMVDYLQLMQVPGVENRVNEISEISRSLKGIAKELNCPVLALSQLNRSLEQRPNKRPVMSDLRESGAIEQDA
DLIVFLYRDEVYNKDSNERGVAEIIIGKQRNGPIGTVRLAFRGEFTRFDDLAPEYYAQLAAGDE
>Mature_464_residues
MNEPMSLDKHLPENLRIPPNSVEAEQAVLGGLLLNNSAWDDVAERVGARDFYRKEHRQIFEVIAQLVEEENPSDLVTVSQ
ALNNLGQLESIGGMTYLSELARNTPSAANITAYSDIVRERSILRQLINVSHKVADSAFNTEGRKSLEILDEAESAIFKIA
EQQKKGDGPQNIKSVLKKAVDRIDELYKTKSSLTGLTSGFEELDKMTGGLQPADMIVVAGRPSMGKTTFAMNLCENVAIK
SNKPVLVFSMEMPAESIVMRMLSSLGRINQGAIRSGNLEKDDWPRITSAIHMLSEQRFFIDDTPALSPLEMRARARRVAR
ECGGELGLIMVDYLQLMQVPGVENRVNEISEISRSLKGIAKELNCPVLALSQLNRSLEQRPNKRPVMSDLRESGAIEQDA
DLIVFLYRDEVYNKDSNERGVAEIIIGKQRNGPIGTVRLAFRGEFTRFDDLAPEYYAQLAAGDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=458, Percent_Identity=58.5152838427948, Blast_Score=553, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51597; Mature: 51597

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEPMSLDKHLPENLRIPPNSVEAEQAVLGGLLLNNSAWDDVAERVGARDFYRKEHRQIF
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
EVIAQLVEEENPSDLVTVSQALNNLGQLESIGGMTYLSELARNTPSAANITAYSDIVRER
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SILRQLINVSHKVADSAFNTEGRKSLEILDEAESAIFKIAEQQKKGDGPQNIKSVLKKAV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DRIDELYKTKSSLTGLTSGFEELDKMTGGLQPADMIVVAGRPSMGKTTFAMNLCENVAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
SNKPVLVFSMEMPAESIVMRMLSSLGRINQGAIRSGNLEKDDWPRITSAIHMLSEQRFFI
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
DDTPALSPLEMRARARRVARECGGELGLIMVDYLQLMQVPGVENRVNEISEISRSLKGIA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KELNCPVLALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADLIVFLYRDEVYNKDSNERG
HHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCCCC
VAEIIIGKQRNGPIGTVRLAFRGEFTRFDDLAPEYYAQLAAGDE
HHHHEEECCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MNEPMSLDKHLPENLRIPPNSVEAEQAVLGGLLLNNSAWDDVAERVGARDFYRKEHRQIF
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
EVIAQLVEEENPSDLVTVSQALNNLGQLESIGGMTYLSELARNTPSAANITAYSDIVRER
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SILRQLINVSHKVADSAFNTEGRKSLEILDEAESAIFKIAEQQKKGDGPQNIKSVLKKAV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
DRIDELYKTKSSLTGLTSGFEELDKMTGGLQPADMIVVAGRPSMGKTTFAMNLCENVAIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCC
SNKPVLVFSMEMPAESIVMRMLSSLGRINQGAIRSGNLEKDDWPRITSAIHMLSEQRFFI
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
DDTPALSPLEMRARARRVARECGGELGLIMVDYLQLMQVPGVENRVNEISEISRSLKGIA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KELNCPVLALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADLIVFLYRDEVYNKDSNERG
HHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCCCCCC
VAEIIIGKQRNGPIGTVRLAFRGEFTRFDDLAPEYYAQLAAGDE
HHHHEEECCCCCCCEEEEEEECCCCCHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]